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Anti-interference film and liver function combined test strip

A technology of combined testing and liver function, applied in the direction of chemical reaction of materials, material analysis by observing the influence of chemical indicators, etc., can solve the problem of uneven de-interference effect, slow heat conduction speed of stuck shell, and deviation of test results. It can improve the anti-interference ability, good anti-interference performance of biological detection and accurate results.

Active Publication Date: 2019-06-18
复星诊断科技(长沙)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The effect of de-interference varies, and AST / ALT indicators are detected separately, which wastes time and resources. However, AST / ALT is tested in different environments or at different time points, or with different sample types (venous, arterial, peripheral blood) of the same person. The ratio of AST / ALT has uncertain influencing factors, which affect clinical judgment
[0004] In addition, most of the multiple blood test cards on the market are stuck and fixed. Since the alanine aminotransferase and aspartate aminotransferase have high requirements for the detection temperature, and the heat conduction speed is slow due to the stuck case, the external environment or the temperature of the test strip causes a large deviation in the test results.
Therefore, there is no traditional jamming method for fixing, and it is difficult to assemble the joint test strip of alanine aminotransferase and aspartate aminotransferase

Method used

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  • Anti-interference film and liver function combined test strip
  • Anti-interference film and liver function combined test strip
  • Anti-interference film and liver function combined test strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Such as figure 1 As shown, a combined liver function test strip includes a diffusion membrane 1, an anti-interference membrane 2, a blood filter membrane 3, a reaction membrane, a chromogenic membrane and a bottom plate 4 stacked in sequence; the reaction membrane includes an ALT reaction membrane 5 and an AST reaction membrane 6. The color-developing film includes an ALT color-developing film 7 and an AST color-developing film 8, between the ALT reaction film 5 and the AST reaction film 6, and between the ALT color-development film 7 and the AST color-development film 8 through an isolation layer 9, ALT The reaction film 5 is set corresponding to the ALT chromogenic film 7, and the AST reaction film 6 is set corresponding to the AST chromogenic film 8; the bottom plate is provided with a first test hole 10 and a second test hole 11, and the ALT chromogenic film 7 covers the first test hole 10. The AST color-developing film 8 covers the second test hole 11 .

[0051] The...

Embodiment 2

[0054] A combined test strip for liver function, comprising a diffusion membrane 1, an anti-interference membrane 2, a blood filter membrane 3, a reaction membrane, a chromogenic membrane and a bottom plate 4 stacked in sequence; the reaction membrane includes an ALT reaction membrane 5 and an AST reaction membrane 6, and The color film includes an ALT color film 7 and an AST color film 8, between the ALT reaction film 5 and the AST reaction film 6, and between the ALT color film 7 and the AST color film 8 through the isolation layer 9, and the ALT reaction film 5 Corresponding to the ALT color-developing film 7, the AST reaction film 6 is set corresponding to the AST color-developing film 8; the bottom plate is provided with a first test hole 10 and a second test hole 11, and the ALT color-developing film 7 covers the first test hole 10, and the AST The color developing film 8 covers the second test hole 11 .

[0055] Wherein, the ALT reaction membrane is obtained by soaking ...

Embodiment 3

[0064] The difference with embodiment 2 is:

[0065] The ALT reaction membrane is obtained by infiltrating the ALT reaction membrane treatment solution. The ALT reaction membrane treatment solution includes: 100mM L-alanine, 5mM α-ketoglutarate, 20g / L protective agent trehalose, 0.5g / L Preservatives and 0.5M PB buffer.

[0066] ALT chromogenic membrane is obtained by treating with ALT chromogenic membrane treatment solution. ALT chromogenic membrane treatment solution includes: 200KU / L pyruvate oxidase, 0.1mM ammonium pyrophosphate, 5mM flavin adenine dinucleotide disodium Salt, 10KU / L peroxidase, 20mM chromogen, 20g / L protective agent BSA, 0.5g / L preservative and 0.5M PB buffer.

[0067] The AST reaction membrane is obtained by infiltrating the AST reaction membrane treatment solution. The AST reaction membrane treatment solution includes: 100mM L-sodium aspartate, 5mM α-ketoglutarate, 20g / L protective agent BSA, 0.5g / L Preservatives and 0.5M PB buffer;

[0068] AST chromo...

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Abstract

The invention provides an anti-interference film and a liver function combined test strip. The anti-interference film is obtained by wetting and treating a substrate with anti-interference treatment fluid. The anti-interference treatment fluid comprises 10-100KU / L of pyruvic oxidase, 100-500KU / L of catalase, 3-30 micronM of cupric salt and 0.1-0.3g / L of erythrocyte antibody. The liver function combined test strip comprises a diffusion film, the anti-interference film, a blood filter film, a reaction film, a color development film and a bottom board, which are stacked up in sequence. The liverfunction combined test strip prepared by the anti-interference film can detect ALT and AST two substances simultaneously and can obtain ALT, AST and ALT / AST three indicators, thereby providing more reliable guidance for clinical diagnosis; and the test strip is simple and fast, adopts smaller matched instruments and is flexible and wide in application.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to an anti-interference membrane and a combined test strip for liver function. Background technique [0002] Alanine aminotransferase (ALT or GPT) and aspartate aminotransferase (AST or GOT) are the most commonly used clinical enzymes to detect liver diseases, and they are also the first choice indicators for clinical detection of liver function impairment. The AST / ALT ratio has certain clinical guiding significance for judging the degree of liver fibrosis. Medical research results show that the AST / ALT ratio gradually increases with the aggravation of liver fibrosis, and when the AST / ALT ratio is ≥1, it has strong guiding significance for diagnosing early liver cirrhosis. [0003] There are two main methods for clinical determination of alanine aminotransferase and aspartate aminotransferase, including kinetic method and chromogenic method. The former is mainly used in the dete...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 张小娜彭勇张梓诺
Owner 复星诊断科技(长沙)有限公司
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