Goat pox reverse indirect blood coagulation diagnostic reagent, and preparation method, and detection method thereof

A diagnostic reagent and goat pox technology, which is applied in the field of goat pox reverse indirect hemagglutination diagnostic reagent and its preparation, can solve the problems of low sensitivity and slow detection speed, and achieve the effect of good specificity and simple detection

Inactive Publication Date: 2011-11-23
GUIZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to overcome the defects of low sensitivity and slow detection speed of the existing methods for detecting goat pox disease, and provide a reverse indirect hemagglutination diagnostic reagent for goat pox and its preparation method. , rapid, accurate and simple detection of goat pox virus antigen

Method used

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Experimental program
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Embodiment Construction

[0012] Goat pox reverse indirect hemagglutination diagnostic reagent is prepared according to the following ratio and method

[0013] 1. Preparation of aldylated red blood cells

[0014] Aseptically collect venous blood from healthy sheep, add 10 times the volume of PBS, centrifuge at 2000 rpm for 15 minutes, wash 5 times with PBS, and prepare 2.5% (volume percentage) red blood cell suspension with phosphate buffer (0.1M, pH 7.4). Then mix the erythrocyte suspension with 1% (volume percent) glutaraldehyde aqueous solution at a volume ratio of 9:1, stir slowly at room temperature for 1 hour, centrifuge at 2000 rpm for 15 minutes, and wash 5 times with phosphate buffer , to remake a 2.5% suspension, then mix the suspension with an equal volume of 1 / 200000 (volume ratio) fresh tannic acid solution, put it in a water bath at 37°C for 1 hour, take it out, and wash it with phosphate buffer for 3 Once, centrifuge at 2000 rpm for 15 minutes to obtain erythrocytes precipitated by alde...

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PUM

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Abstract

The invention discloses a goat pox reverse indirect blood coagulation diagnostic reagent and a preparation method thereof. The content of sensitized erythrocyte in the blood coagulation reagent is 0.8 to 1.0 percent; and the purified goat pox IgG content is 1.549mg/ml. Compared with the conventional common agar diffusion experiment, the preparation method has the advantages that: the goat pox reverse indirect blood coagulation diagnostic reagent is prepared by sensitizing hydroformylation sheep red blood cells by using the purified goat pox IgG, so that a goat poxvirus antibody can be detected quickly, accurately and simply; and the goat pox reverse indirect blood coagulation diagnostic reagent has higher specificity, sensitivity and repeatability and can be widely applied to quick veterinary clinical detection of the goat poxvirus antibody and provide a basis for disease prevention, diagnosis and control.

Description

technical field [0001] The invention relates to the technical field of reverse hemagglutination diagnostic reagents, in particular to a goat pox reverse indirect hemagglutination diagnostic reagent and a preparation method and a detection method thereof. Background technique [0002] Goat pox is an endemic highly contagious infectious disease caused by goat poxvirus (GTPV), which can occur all year round and is common in spring and autumn, regardless of gender, age, or species. The World Organization for Animal Health (OIE) listed goat pox as a major infectious disease of category A, and in 1999, the Ministry of Agriculture of the People's Republic of China announced goat pox as a national first-class animal disease. In recent years, more than 20 provinces and cities in my country have successively reported the occurrence of goat pox epidemic, which has caused great economic losses and posed a serious threat to the booming goat breeding industry in my country. The tradition...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N33/569
Inventor 刘忠伟周碧君程振涛文明王开功嵇辛勤岳筠汪德生
Owner GUIZHOU UNIV
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