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Application of verbascoside in preparation of pneumonia treatment drug

A technology of verbascoside and pneumonia, applied in the field of medicine, can solve problems such as treatment failure

Inactive Publication Date: 2016-03-23
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current drugs for the treatment of pneumococcal pneumonia usually use β-lactam antibiotics, but the emergence of penicillin-resistant and vancomycin-resistant pneumococci often leads to treatment failure

Method used

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  • Application of verbascoside in preparation of pneumonia treatment drug
  • Application of verbascoside in preparation of pneumonia treatment drug
  • Application of verbascoside in preparation of pneumonia treatment drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0007] 1. Hemolysis test

[0008] The purified pneumolysin ply was serially diluted twice in a 96-well plate, and 10 7 Sheep red blood cells were incubated at 37°C for 10 min and then centrifuged. The highest dilution factor that lysed red blood cells was regarded as the hemolytic titer (Hemolysistitre).

[0009] The hemolytic titers of hemolysin ply without adding verbascoside and adding different concentrations of verbascoside are as follows: Table 1:

[0010] Table 1. Inhibitory effect of verbascoside on the hemolytic activity of pneumolysin

[0011]

[0012] 2. Protection test of human lung epithelial cells (A549) injury

[0013] A549 cells were cultured in DEMED medium (containing 10% fetal bovine serum) at 37°C with 5% CO 2 Cultured to 1.5 x 10 4 Cells / well were plated in a 96-well cell culture plate, 100 μl per well. After the cells adhere to the wall, add 0.5 μl of purified pneumolysin protein, and add different concentrations of verbascoside, and place in a c...

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Abstract

The invention relates to application of verbascoside in preparation of a pneumonia treatment drug. The treatment effect of the verbascoside to streptococcus pneumoniae infection is proved through sheep red blood cell hemolysis test, protection experiment of the human pulmonary epithelial cell (A549) damage and a mouse streptococcus pneumoniae pneumonia pneumonia model. Compared with antibiotic treatment, when the verbascoside is used for treatment, so that the pneumonia treatment drug has the advantages that no drug resistance exists and the curing rate is high.

Description

technical field [0001] The invention relates to the application of verbascoside in the preparation of medicines for treating pneumonia, and belongs to the field of medicine. 1. Background Technology [0002] Verbascoside is a phenylethanol glycoside compound isolated from Cistanche deserticola and Zizhu leaves. Studies have shown that the drug has pharmacological properties such as immune regulation, neuroprotection, liver protection, kidney tonifying and impotence, anti-inflammation and anti-platelet aggregation. effect. But so far there are no reports of verbascoside being used in the treatment of bacterial pneumonia at home and abroad. [0003] Bacterial pneumonia is a disease caused by infections such as Streptococcus pneumoniae, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pyogenes, Legionella, etc. The mortality rate of this disease is extremely high in children, the elderly and immunocompromised patients. In recent years, the extensive use of antibio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7034A61P11/00A61P31/04A61P31/10
CPCA61K31/7034
Inventor 邓旭明赵小然牛效迪李洪恩
Owner JILIN UNIV
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