The invention provides a loop-mediated isothermal amplification quick detecting kit for vibrio harveyi. The loop-mediated isothermal amplification quick detecting kit comprises one reactive liquid A, wherein the reactive liquid A comprises the following components: 10 * Lampbuffer, dNTP, a magnesium sulfate water solution, a glycine betaine water solution, a primer vh-F3, vh-B3, vh-FIP, vh-BIP, Bst DNA (Deoxyribose Nucleic Acid)enzyme, 10 * fluorochrome SYBRGreen I, a positive control sample, a negative control sample and ultrapure water. The invention also relates to a detecting method using the kit. The kit has the advantages of high specificity, high sensitivity, fast speed, low cost and simpler operation method, and is suitable for quickly detecting an aquaculture site. With adoption of the kit and the method, defects of long time, high workload, cross contamination, complex in operation and demands on complex instruments in the prior art can be solved; a new technical platform is provided for detecting diseases of aquatic livestock; and urgent needs on site detecting of diseases caused by the vibrio harveyi at present can be met better.