Method for diagnosing avian influenza virus by means of RT-PCR (reverse transcription-polymerase chain reaction) and ELISA (enzyme-linked immunosorbent assay) and kit using method

A bird flu virus and diagnostic method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as blanks, achieve the effects of eliminating pollution, facilitating automation, and strong specificity
CN102495208AInactive Publication Date: 2012-06-13INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
3 Cites 7 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
Publication Date
2012-06-13
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention provides a special prime designed according to conservative M genes of influenza virus. By the aid of the principle of specific binding of biotin and streptavidin, biotin-labeled PCR (polymerase chain reaction) products are bound on a microtiter plate, anti-digoxin peroxidase is added, and finally TMB (tetramethylbenzidine) color development solution is added, so that positive products can develop colors. On the basis, a special kit is successfully prepared. The method combines PCR with a high-sensitivity digoxin detection system, the sensitivity of the method is 100 times higher than that of a conventional agarose gel electrophoresis detection method, and the method is high in specificity and overcomes difficulty in detecting AIV (avian influenza virus) of a low content in a tissue sample. Compared with a virus isolation method, the method has the advantages that coincidence rate can reach more than 95%, pollution of chemical agents (such as ethidium bromide) is eliminated, automation is benefited, the method is suitable for detecting a large number of samples, and a new approach is provided for early diagnosis of avian influenza and molecular epidemiological investigation.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the field of animal virology, in particular to diagnostic technology in the field of biotechnology, and more specifically to a high-throughput rapid diagnosis of avian influenza virus using RT-PCR ELISA technology and a kit prepared by applying the diagnostic method. Background technique

[0002] Avian influenza is an acute contact infectious disease caused by type A influenza virus, which can infect many animals including humans, poultry, pigs, and horses. Avian influenza not only brings catastrophic damage to the livestock industry, but also poses a serious threat to public health. Avian influenza virus (AIV) mutates rapidly. Although it has not yet acquired the ability to effectively spread among humans, its increasingly frequent human infection events indicate that AIV virus is undergoing a complex process of mutation to adapt to the human body. Once again, the human A flu outbreak is already brewing. Long-term epidemiolo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More