Method for synchronously detecting multiple antibiotics in polluted water

A technology of simultaneous detection and antibiotics, applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as antibiotic pollution and farmland soil environmental pollution, and achieve the effect of low detection limit, high detection accuracy, and good practical application prospects.

Inactive Publication Date: 2018-01-09
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Therefore, for hospital sewage or urban domestic sewage containing antibiotics, whether it has been treated or not, as long as it is discharged, it may cause pollution to surface water, groundwater and farmland soil environment
In addition, a considerable amount of antibiotics are polluted every year due to expired discards

Method used

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  • Method for synchronously detecting multiple antibiotics in polluted water
  • Method for synchronously detecting multiple antibiotics in polluted water
  • Method for synchronously detecting multiple antibiotics in polluted water

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The mensuration of embodiment 1 standard curve, detection limit, limit of quantification and rate of recovery

[0039] (1) Preparation of antibiotic standard stock solution

[0040] Antibiotics to be tested include tetracycline (TC), 4-epitetracycline hydrochloride (E-TC), chlortetracycline (CTC), epitetracycline hydrochloride (E-CTC), oxytetracycline (OTC), 4- Epitropic oxytetracycline (E-OTC), penicillin (PCN), ampicillin (AMP), cefotaxime (CTX), neospiramycin (NSPM), spiramycin (SPM), clarithromycin ( CLR), Tylosin (TLY), Roxithromycin (NERY), Norfloxacin (NOR), Ciprofloxacin (CIP), Tilmicosin (TIL), Enrofloxacin (ENR) , ofloxacin (OFX), sulfamethazine (SMZ), sulfamethoxine (SMX), sulfamethizole (SML), sulfadiazine (SD) and sulfamethazine (SMN), a total of 24 kinds . Accurately weigh 0.010g of each drug, dissolve it with methanol and dilute it to a 10mL brown volumetric flask, mix well to obtain a stock solution with a mass concentration of 1000mg L-1, store it in...

Embodiment 2

[0064] The detection of 24 kinds of antibiotics in the livestock and poultry breeding waste water of embodiment 2

[0065] Detect a certain waste water body sample with the method and condition identical with embodiment 1, experimental procedure is as follows:

[0066] (1) Take 100mL sample of the measured pig farm wastewater at 5000r·min -1 Centrifuge for 10 min, take the supernatant and pass through a 0.45 μm filter membrane, and use 1mol L -1 hydrochloric acid to adjust the pH of the filtrate to 3.85, add 100 μL saturated Na 2 EDTA solution.

[0067] (2) SPE solid-phase extraction column (Oasis HLB column, 6mL / 500mg), pre-activated by rinsing with 5mL ethyl acetate, 5mL methanol, and 5ml pure water with pH=3.85. Turn on the vacuum pump and control the flow rate to about 3-5mL min -1 The membrane supernatant was applied to the column with a loading volume of 5 mL. After passing through the column, wash the small column with 5mL 5% methanol water and 5mL ultrapure water ...

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Abstract

The invention discloses a method for synchronously detecting multiple antibiotics in polluted water. The method comprises the following steps: (1) after carrying out solid-liquid separation on to-be-detected polluted water, taking supernatant, carrying out membrane filtration, and regulating the pH (Potential of Hydrogen) value of filtrate to be X, wherein X is an arbitrary value within 3.5 to 4;(2) enabling the filtrate of which the pH value is regulated to pass through an HLB (Hydrophile-Lipophile Balance) column for enrichment, and extracting after completing column chromatography isolation, thus obtaining an eluent of antibiotics; (3) detecting the content of the antibiotics by adopting HPLC (High Performance Liquid Chromatography)-MS (Mass Spectrometry) method. The method disclosed by the invention is low in detection limit and high in detection accuracy, is capable of synchronously detecting more than 20 kinds of antibiotics, and has a very good application prospect.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry detection, in particular to a method for synchronous detection of multiple antibiotics in polluted water, which can be applied to the fields of water quality detection, biomedicine and the like. Background technique [0002] Antibiotics have been used around the world since the early 1940s. The migration and transformation of antibiotics in water and soil in the environment has caused great pressure on the environment, and the resulting bacterial resistance has become an important problem threatening human health. In recent years, research on antibiotic pollution in the environment has gradually become a hot spot in the academic field. The research objects of the main pollution sources of antibiotics have developed from pharmaceutical wastewater and hospitals with large discharges to urban sewage treatment plants and livestock and poultry breeding (Heberer and Stan, 1997). Internati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/08G01N30/14
Inventor 陈梅雪柴玉峰王瑞柳蒙蒙魏源送
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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