Kit (Latex-enhanced immunoturbidimetry) for detecting content of glycocholic acid in blood serum

An immunoturbidimetric and latex-enhanced technology, applied in the field of biochemistry, can solve the problems of expensive reagents, long detection time, complicated operation, etc., and achieve the effects of reliable detection results, shortened detection time, and wide linear range.
CN102944673AActive Publication Date: 2013-02-27CO HEALTH BEIJING LAB
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Applications(China)
Current Assignee / Owner
CO HEALTH BEIJING LAB
Publication Date
2013-02-27

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Abstract

The invention relates to a kit of latex-enhanced immunoturbidimetry for detecting content of glycocholic acid in blood serum. Specifically, the provided glycocholic acid detection kit comprises a reagent R1, a reagent R2 and a calibrator, wherein the reagent R1 comprises a reaction promotion agent, a preservative, a surface active agent, a stabilizer, an electrolyte and a buffer solution; the reagent R2 comprises latex particles combined with a glycocholic acid antibody, a preservative, a surface active agent, a stabilizer, an electrolyte and a buffer solution; and the calibrator comprises a preservative, an electrolyte, a stabilizer, glycocholic acid pure products and a buffer solution. The kit for detecting the content of the glycocholic acid in the blood serum, disclosed by the invention, ensures the high sensitivity and wide linear range of a kit by utilizing a method of coating the latex particles by utilizing polyclonal antibodies, also has the advantages of high accuracy, good repeatability, strong specificity, simplicity in operation and the like, and can be applied to a clinical general full automatic biochemical analyzer.
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Description

technical field

[0001] The invention relates to the technical field of biochemistry, in particular to a kit for measuring glycocholic acid (CG) content in human serum by using a latex-enhanced immune turbidimetric method. Background technique

[0002] Serum glycocholic acid (CG) is one of the conjugated bile acids in which bile acid and glycine are combined twice. In liver cells, cholesterol is transformed into primary bile acid through extremely complicated enzymatic reactions. Among them are cholic acid (CA) and chenodeoxycholic acid (CD-CA). There are three hydroxyl groups (C3, C7, C12) on the steroid core of cholic acid, and the hydroxyl group at the end of the side chain is combined with glycine through a peptide bond, with a molecular weight of 462U.

[0003] The normal metabolic pathway of CG is the entero-hepatic circulation. CG is synthesized by liver cells, discharged into the gallbladder through the capillaries and bile ducts, and enters the duodenum along with b...

Claims

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