114 results about "Glycocholic acid" patented technology

The invention relates to a glycocholic acid detecting reagent and a preparing method and a detecting method thereof, in particular to a glycocholic acid immunodetection reagent and a preparing method and a detecting method thereof. The glycocholic acid immunodetection reagent comprises a glycocholic acid specificity resisting antibody and an indicating reagent, wherein the indicating reagent is used for detecting the glycocholic acid specificity resisting antibody and a glycocholic acid composite; the glycocholic acid specificity resisting antibody is obtained from glycocholic acid immunogen immune animals. The glycocholic acid immunodetection reagent has the benefits that the glycocholic acid immunogen specificity is strong, the immunogenicity is high, and the prepared glycocholic acid specificity resisting antibody has strong specificity and high valence and does not have any cross reaction with 45 common drugs; a homogeneous enzyme immunodetection reagent containing the glycocholic acid specificity resisting antibody can conveniently, rapidly and accurately determine the content of glycocholic acid in a sample and can simultaneously test multiple samples on a fully-automatic biochemical analysis instrument; the high-throughout rapid measurement of the glycocholic acid is realized, the accuracy is high, the specificity is strong, and both the precision and the detection efficiency are greatly improved.

The invention relates to a kit of latex-enhanced immunoturbidimetry for detecting content of glycocholic acid in blood serum. Specifically, the provided glycocholic acid detection kit comprises a reagent R1, a reagent R2 and a calibrator, wherein the reagent R1 comprises a reaction promotion agent, a preservative, a surface active agent, a stabilizer, an electrolyte and a buffer solution; the reagent R2 comprises latex particles combined with a glycocholic acid antibody, a preservative, a surface active agent, a stabilizer, an electrolyte and a buffer solution; and the calibrator comprises a preservative, an electrolyte, a stabilizer, glycocholic acid pure products and a buffer solution. The kit for detecting the content of the glycocholic acid in the blood serum, disclosed by the invention, ensures the high sensitivity and wide linear range of a kit by utilizing a method of coating the latex particles by utilizing polyclonal antibodies, also has the advantages of high accuracy, good repeatability, strong specificity, simplicity in operation and the like, and can be applied to a clinical general full automatic biochemical analyzer.

The invention discloses a polyene phosphatidyl choline injection and the preparation method. The components and the ratio (portion) of the polyene phosphatidyl choline injection of the invention is: 465 portion of polyene phosphatidyl choline injection, 88 portion of benzyl zlcohol, 50 to 800 portion of glycocholic acid, cholic acid or tween-80, 50 to 80 portion of alcohol, propanediol or glycerin, 15 to 200 portion of sodium hydroxide or sodium carbonate, 0.5 to 5 portion of 2,6-D-itert-butyl-p-cresol, 0.8 to 8 portion of Tertiary butyl-4-hydroxyl anisole, 3 to 25 portion of Vitamin E by weight. The polyene phosphatidyl choline injection of the invention has good clarity, high stability, simple preparation process and easy operation.

The invention relates to a liposome lyophilized preparation prepared from twelve compound vitamins. The lyophilized preparation is characterized in that the lyophilized preparation is prepared from a liposome which consists of soybean lecithin and glycocholic acid and is encapsulated with twelve vitamins of retinyl palmitate, cocarboxylase tetrahydrate, riboflavin sodium phosphate, pyridoxine hydrochloride, cyanocobalamin, cholecalciferol, ascorbic acid, racemization-alpha tocopherol, D-biotin, niacinamide, folacin and dexpanthenol.

Disclosed is a prediction method of glomerular filtration rate (GFR) from urine samples after kidney transplantation to provide an information needed for predict renal function after the transplantation, more particularly to a prediction method of glomerular filtration rate (GFR) from urine samples after kidney transplantation, which comprises detecting metabolic profiles of five biomarkers, 5a-androst-3-en-17-one (AS), glycocholic acid (GC), sphingosine (SG), tryptophan (TR) and histidine (HT), from urine samples of patients. Glomerular filtration rate (GFR) after kidney transplantation can be predicted more rapidly and precisely to provide an information needed for predict renal function after the transplantation by using five metabolites as biomarkers. The method provides more specific, sensitive, and reliable biomarkers that monitor clinical outcomes and adverse renal events after kidney transplantation, such as rejection, drug toxicity, delayed graft function, and infection.

The invention discloses a preparation method of a kit for measuring glycocholic acid content in the human body. The kit comprises a glycocholic acid R1 reagent, a glycocholic acid R2 reagent and a glycocholic acid calibrator. The glycocholic acid R1 reagent is obtained by sufficiently mixing glycocholic acid-protein conjugate with buffer liquid, the glycocholic acid R2 reagent is obtained by mixing latex particles wrapped by a glycocholic acid-protein antibody with suspension buffer liquid, and the glycocholic acid calibrator is composed of human-derived glycocholic acid and buffer liquid. The preparation method comprises the steps that human plasma containing glycocholic acid, a serum sample and the glycocholic acid-protein conjugate are competitively bound with the glycocholic acid-protein antibody latex particles, the glycocholic acid-protein antibody latex particles bound with the glycocholic acid do not produce turbidity, while, the glycocholic acid-protein antibody latex particles bound with the glycocholic acid-protein conjugate will produce turbidity changes, and quantitative analysis on the glycocholic acid is carried out by measuring the lowering degree of the system turbidity after the reaction. By means of the preparation method, the immunoreaction signal intensity is greatly improved, and even low-content matter can produce strong turbidity reaction during immune binding for detection.

The invention relates to a determination reagent for glycocholic acid and a preparation method of the determination reagent, and belongs to the technical field of biochemical means testing. The determination reagent comprises an R1 reagent, an R2 reagent and a glycocholic acid standard sample; a solution formed by dissolving a sulfo-oxidation type coenzyme into purified water added with a buffer solution is adopted as the R1 reagent; a solution formed by dissolving a reduction type coenzyme into purified water added with a buffer solution, glycocholic acid dehydrogenase and sodium azide is adopted as the R2 reagent; a solution formed by dissolving a sodium glycocholate hydrate, a buffer solution and sodium azide into purified water is adopted as the glycocholic acid standard sample. The determination reagent is applied to glycocholic acid determination and has the advantages of being quick, sensitive, good in accuracy, high in specificity, good in stability and the like.

The invention discloses a reagent kit for measuring glycocholic acid in human serum. The reagent kit comprises glycocholic acid reagents R1, glycocholic acid reagents R2 and glycocholic acid standard substances. The reagent kit has the advantages that competition processes are used in detection procedures, accordingly, variation of the turbidity of test solution can be controlled in wide ranges, the glycocholic acid can be detected by the aid of common analytical instruments, and the reagent kit is favorable for guaranteeing the accuracy of the instruments.

The invention discloses a kit. The kit comprises a reagent R1 and a reagent R2, the reagent R1 contains a trihydroxymethyl aminomethane (Tris-HCL) buffer solution, an anti-taurine antibody, an anti-glycocholic acid antibody and glucose-6-phosphate (G6P), and the reagent R2 contains the trihydroxymethyl aminomethane (Tris-HCL) buffer solution, a glycocholic acid-G-6-PD conjugate, NADP and BSA. The invention further discloses a preparation method of the kit, particularly discloses a preparation method of the anti-taurine antibody the anti-glycocholic acid antibody and further discloses a method for detecting peripheral blood glycocholic acid through the kit. Accordingly, interference of taurocholic acid can be avoided, the detection sensitivity reaches up to 0.1 microgram per milliliter, and the advantages of high specificity and detection repeatability, good stability and the like are achieved.

The invention discloses a diagnosis marker suitable for an early-stage esophageal squamous cell cancer diagnosis. The diagnosis marker is a composition of two or more than two kinds of materials from the following five kinds of serum metabolic markers including L-tyrosine, L-tryptophan, glycocholic acid, taurocholate and cortisol. When the diagnosis marker provided by the invention is adopted, a diagnosis model can be built. The diagnosis model has the advantages of good effect, high sensitivity and good specificity, is suitable for late-stage and early-stage esophagus cancer diagnoses, and has good clinic use and popularization value.