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Kit for determining glycocholic acid in human blood

A technology of glycocholic acid and a kit, which is applied in the field of kits for measuring glycocholic acid in human blood, can solve the problems of cumbersome operation, slow measurement speed, lack of reagents and the like, and achieves simple inspection operation, slow measurement speed and good sensitivity. Effect

Active Publication Date: 2013-03-06
浙江强盛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1. Elisa assay method: This method is generally used for semi-quantitative assays, and its accuracy, sensitivity, and precision are poor, and the operation is cumbersome, which is not conducive to Widely used in clinical
[0005]2. Chemiluminescence measurement method: This method has high sensitivity, but the measurement accuracy and reagent stability are poor, and special chemiluminescence measurement equipment is required, and the measurement speed is relatively high. Slow, with obvious limitations in clinical application
[0007] At present, there is a shortage of glycocholic acid determination kits suitable for clinical use in the market in my country, especially the lack of reagents that can be accurately, easily and quickly determined, and can simultaneously measure a large number of specimens

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A test kit for measuring glycocholic acid in human blood, comprising reagent A, reagent B and a known concentration of glycocholic acid calibrator, wherein the mass ratio of reagent A to reagent B is 5: 1;

[0036] Each component of described reagent A and content thereof are as follows:

[0037] Microbial inhibitor (sodium azide) 0.001g / L, EDTA 5.0 g / L, glycerol 0.1 g / L, bovine serum albumin 5.0g / L, surfactant (Tween20) 3.0g / L, reaction enhancer (poly Ethylene glycol 2000) 0.5g / L, sodium chloride 8.0g / L, pH 6 phosphate buffer 25mmol / L.

[0038] Each component and content thereof of described reagent B are as follows:

[0039] Microbial inhibitor (sodium azide) 0.001g / L, EDTA 5.0 g / L, glycerol 0.1 g / L, bovine serum albumin 5.0g / L, surfactant (Tween20) 3.0g / L, reaction enhancer (poly Ethylene glycol 2000) 0.5g / L, sodium chloride 8.0g / L, pH 6 phosphate buffer 25mmol / L, nano-microspheres cross-linked with glycocholic acid monoclonal antibody with a particle size of 50nm ...

Embodiment 2

[0050] A test kit for measuring glycocholic acid in human blood, comprising reagent A, reagent B and a known concentration of glycocholic acid calibrator, wherein the mass ratio of reagent A to reagent B is 4: 1;

[0051] Each component of described reagent A and content thereof are as follows:

[0052] Microbial inhibitor (ProClin 200) 0.5g / L, EDTA 2.0 g / L, glycerol 5 g / L, bovine serum albumin 2.0g / L, surfactant (Tween30) 1.0g / L, reaction enhancer (polyethylene Diol 4000) 2g / L, sodium chloride 3.0g / L, pH 7 glycine buffer 50mmol / L.

[0053] Each component and content thereof of described reagent B are as follows:

[0054] Microbial inhibitor (ProClin 200) 0.5g / L, EDTA 2.0 g / L, glycerol 5 g / L, bovine serum albumin 2.0g / L, surfactant (Tween30) 1.0g / L, reaction enhancer (polyethylene Glycol 4000) 2g / L, sodium chloride 3.0g / L, glycine buffer solution at pH 7 50mmol / L, and nano-microspheres cross-linked with glycocholic acid monoclonal antibody with a particle size of 80nm is 0.2...

Embodiment 3

[0064] A test kit for measuring glycocholic acid in human blood, comprising reagent A, reagent B and a known concentration of glycocholic acid calibrator, wherein the mass ratio of reagent A to reagent B is 1:1;

[0065] Each component of described reagent A and content thereof are as follows:

[0066] Microbial inhibitor (ProClin 300) 1.1g / L, EDTA 0.05 g / L, glycerin 10 g / L, bovine serum albumin 1.0g / L, surfactant (Tween80) 0.01g / L, reaction enhancer (polyethylene Diol 8000) 5.0g / L, sodium chloride 0.1g / L, pH 8 N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid buffer 100mmol / L.

[0067] Each component and content thereof of described reagent B are as follows:

[0068] Microbial inhibitor (ProClin 300) 1.1g / L, EDTA 0.05 g / L, glycerin 10 g / L, bovine serum albumin 1.0g / L, surfactant (Tween80) 0.01g / L, reaction enhancer (polyethylene Diol 8000) 5.0g / L, sodium chloride 0.1g / L, pH 8 N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid buffer 100mmol / L, and glycocholic aci...

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Abstract

The invention discloses a kit for determining glycocholic acid in human blood. The kit comprises a reagent A, a reagent B and a calibrator for glycocholic acid with known concentration, wherein the mass ratio of the reagent A to the reagent B is (1-5):1; the reagent A comprises a microorganism inhibiting agent, EDTA (Ethylene Diamine Tetraacetic Acid), glycerol, albumin bovine serum, a surface active agent, a reactive reinforcing agent, sodium chloride and a Good's buffer solution with the pH of 6-8; and the reagent B comprises the microorganism inhibiting agent, the EDTA (Ethylene Diamine Tetraacetic Acid), the glycerol, the albumin bovine serum, the surface active agent, the reactive reinforcing agent, sodium chloride, the Good's buffer solution with the pH of 6-8 and nanoparticles crosslinked with a glycocholic acid monoclonal antibody. The kit is simple, convenient and fast in detection and operation, can be used for simultaneously and quickly determining a large amount of samples and has good detection accuracy and sensitivity.

Description

technical field [0001] The invention relates to an immunoassay kit, in particular to a kit for measuring glycocholic acid in human blood. Background technique [0002] Cholyglycine (CG) is a conjugated bile acid formed by combining bile acid and glycine. Cholic acid (CA) and chenodeoxycholic acid (CDCA) are collectively referred to as bile acids. Among the biochemical indicators of liver injury, CG is more sensitive than alanine aminotransferase, bilirubin and other tests. In acute hepatitis, chronic active hepatitis, primary liver cancer, liver cirrhosis and chronic persistent hepatitis, the levels of serum CG were higher than those in the normal control group, and the positive rate increased in descending order. Especially in the clinical differentiation of chronic active hepatitis and chronic persistent hepatitis, CG can be used as a valuable indicator, and the amplitude and frequency of CG elevation in chronic active liver are higher than those in chronic migratory liv...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/531
Inventor 黄信用
Owner 浙江强盛生物科技有限公司
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