63 results about "Serum albumin protein" patented technology

The invention discloses a kit for determining glycocholic acid in human blood. The kit comprises a reagent A, a reagent B and a calibrator for glycocholic acid with known concentration, wherein the mass ratio of the reagent A to the reagent B is (1-5):1; the reagent A comprises a microorganism inhibiting agent, EDTA (Ethylene Diamine Tetraacetic Acid), glycerol, albumin bovine serum, a surface active agent, a reactive reinforcing agent, sodium chloride and a Good's buffer solution with the pH of 6-8; and the reagent B comprises the microorganism inhibiting agent, the EDTA (Ethylene Diamine Tetraacetic Acid), the glycerol, the albumin bovine serum, the surface active agent, the reactive reinforcing agent, sodium chloride, the Good's buffer solution with the pH of 6-8 and nanoparticles crosslinked with a glycocholic acid monoclonal antibody. The kit is simple, convenient and fast in detection and operation, can be used for simultaneously and quickly determining a large amount of samples and has good detection accuracy and sensitivity.

This invention is related to an adhesive composition which may be used to bond or seal tissue in vivo. The adhesive composition is readily formed from a two component mixture which includes a first part of a protein, preferably a serum albumin protein, in an aqueous buffer having a pH in the range of about 8.0-11.0 and a second part of a water-compatible or water-soluble bifunctional crosslinking agent. When the two parts of the mixture are combined, the mixture is initially a liquid which cures in vivo on the surface of tissue in less than about one minute to give a strong, flexible, pliant substantive composition which bonds to the tissue and is absorbed in about four to sixty days. The adhesive composition may be used either to bond tissue, to seal tissue or to prevent tissue adhesions caused by surgery.

This invention is related to an adhesive composition which may be used to bond or seal tissue in vivo. The adhesive composition is readily formed from a two component mixture which includes a first part of a protein, preferably a serum albumin protein, in an aqueous buffer having a pH in the range of about 8.0-11.0 and a second part of a water-compatible or water-soluble bifunctional crosslinking agent. When the two parts of the mixture are combined, the mixture is initially a liquid which cures in vivo on the surface of tissue in less than about one minute to give a strong, flexible, pliant substantive composition which bonds to the tissue and is absorbed in about four to sixty days. The adhesive composition may be used either to bond tissue, to seal tissue or to prevent tissue adhesions caused by surgery.

The invention belongs to the technical field of functional nanometer materials, in particular to a water-phase preparation method of a near-infrared luminescent silver sulfide quantum dot. The preparation method is a normal-temperature and normal-pressure water-phase preparation method and comprises the following steps of: with silver nitrate, bovine serum albumin and sodium sulfide as raw materials, regulating and controlling the nucleation and growth of a nanometer material by using protein, and eliminating unreacted ions through dialysis via a semipermeable membrane to obtain an extra-small (smaller than 10 nanometers) silver sulfide quantum dot stably existing in a water solution. In the preparation process, water is used as a solvent, conditions are mild, operability is strong, an extra-small quantum dot with good luminescent window and better biocompatibility is obtained under extremely mild reaction conditions, and the quantum dot is hopeful to achieve broad application prospect in the aspect of living organism imaging.

The invention relates to an immobilization method of bovine serum albumin taking poly-dopamine/oxidized graphene as a second-order reaction platform in a micro-fluidic chip channel and chiral separation application of the bovine serum albumin, and belongs to the technical field of micro-fluidic chips. The immobilization method comprises the following steps of: pumping a dopamine and oxidized graphene mixed solution into a micro-fluidic chip separation channel by using a vacuum pump, wherein the dopamine fixes the oxidized graphene to the surface of the micro-fluidic chip separation channel to form a poly-dopamine/oxidized graphene film in a self-polymerization process; combining the bovine serum albumin with the surface of poly-dopamine/oxidized graphene under the action of Pi-Pi, a hydrogen bond, hydrophobicity, and the like between the poly-dopamine/oxidized graphene and the bovine serum albumin to obtain a poly-dopamine/oxidized graphene/ bovine serum albumin functionalized micro-fluidic chip channel. The bovine serum albumin has good stability, biocompatibility and hydrophilicity, can be used for enhancing the loading capacity of the bovine serum albumin in the micro-fluidic chip separation channel, keeping the biological activity of the bovine serum albumin and providing the universal platform for the high-efficiency separation of an amino acid antipode and a dipeptide antipode.

The invention discloses a kit capable of quantitatively detecting soluble B7-H1, which comprises a horseradish peroxidase label, tetramethylbenzidine serving as a reaction substrate, bovine serum albumin, an elisa plate, washing liquor, stop solution, a coating antibody, a standard protein and a detection antibody, wherein the coating antibody is a mouse anti-human B7-H1 monoclonal antibody and the nucleotide sequence of the heavy chain of the coating antibody is represented by a nucleotide sequence SEQ ID No.3 in the sequence list, and the nucleotide sequence of the light chain of the coating antibody is represented by a nucleotide sequence SEQ ID No.4 in the sequence table. The kit capable of quantitatively detecting soluble B7-H1 has high specificity and can be used for accurately quantitative analysis on soluble B7-H1 protein factor concentration in liquid for human cell culture supernate, serum, plasma, hydrothorax and the like.

The invention discloses a conductive and heat-conducting glass fiber composite material coated with dopamine and modified by nano-silica, which is characterized in that a buffer solution is added to the nano-silica, ultrasonically dispersed, dopamine is added, reacted in a water bath, centrifuged, Washing and drying; adding KH570 and ethanol to micron aluminum nitride, adjusting pH, ultrasonic oscillation, heating and stirring, reflux reaction, vacuum filtration after cooling, washing with water, drying, grinding and crushing to obtain modified aluminum nitride; glass fiber After calcination, soak in acetone for cleaning treatment, add hydrochloric acid solution, hydroxylation treatment, wash with distilled water, dry, add bovine serum albumin solution, stir for reaction, wash with water, and dry to obtain bovine serum albumin modified glass fiber Adding modified glass fiber to the graphite oxide dispersion, stirring, pulling, washing, drying, steam treatment, heat treatment, and then mixing with the material obtained above to obtain a conductive and thermally conductive material.

The invention discloses an amperometric DNA (deoxyribonucleic acid) electrochemical sensor based on a protein controlled assembling interface. The amperometric DNA electrochemical sensor comprises an electrode, proteins, a capture probe DNA, a signal probe DNA and horse radish peroxidases, wherein the electrode is a gold electrode, the proteins are bovine serum albumins, the capture probe DNA is a 5' terminated mercapto modified single-strand DNA, the signal probe DNA is a 3' terminated biotin labeled single-strand DNA, and the horse radish peroxidases are labeled by avidin. The amperometric DNA electrochemical sensor is characterized in that the bovine serum albumins are formed into a sequentially-assembled bovine serum albumin molecule layer on the surface of the gold electrode, and the capture probe DNA is assembled by using sequential holes among bovine serum albumin molecules in the bovine serum albumin molecule layer. The amperometric DNA electrochemical sensor is used for carrying out detection on a target DNA, and the detection limit is 0.01pmol/L. Compared with similar methods, the amperometric DNA electrochemical sensor constructed by using the method is high in sensitivity and good in repeatability.

A synthesis method of an universal artificial antigen of alkylphenol medicines belongs to the technical field of biochemical engineering. The synthesis method is as follows: nonyl phenol (NP) is used as hapten, and the active ester method is adopted to couple the hapten and the carrier protein bovine serum albumin (BSA) to obtain the universal artificial antigen of alkylphenol medicines. Polyacrylamide gel electrophoresis is adopted to detect the coupling ratio of the conjugate. By adopting the method, the universal artificial antigen of alkylphenol medicines can be synthesized successfully; the synthesis step is concise and effective; and the method can be completely used in immunoassay, provide the necessary artificial antigen for the future research of people and meet the need of the domestic research on the artificial antigen.

The invention discloses a bovine serum albumin nano microsphere preparation method, and provides a protein nano microsphere cross-linking method based on protein dimerization. According to the method, bovine serum albumin is used as a matrix material, and through the protein dimerization and inverse solution method, bovine serum albumin nano microsphere cross-linking can be realized to obtain a nano microsphere drug delivery material with structural stability. The method avoids the use of a toxic chemical crosslinking agent, and guarantees the use safety of the delivery system, and biological activity of the entrapped drugs is expected to retain, so that the the nano microsphere system biocompatibility can be significantly improved. The method is simple and easy in process and equipment and safe to operate, has the advantages of low preparation temperature, short processing cycle and the like, and has the application value in the fields of embedding and transfering of active drugs, proteins or gene.

The invention relates to synthesis and an application of an envelope antigen universal for pyrethriods pesticide, belonging to the technical field of biochemical engineering. The envelope antigen universal for pyrethriods pesticide is synthesized by taking 3-phenoxybenzoic acid and nitrobenzene alanine as raw material through two steps of chemical reaction, the envelope antigen and a carrier protein BSA (Bovine Serum Albumin) are coupled through diazotization, and the coupling ratio of a conjugate is identified and estimated through ultraviolet spectroscopy. The synthesized conjugate (envelope antigen) is used for enzyme linked immunoassay and preparation of a collaurum immunochromatography test strip. The enzyme linked immunoassay and the preparation of the collaurum immunochromatography test strip prove that the synthesis of the envelope antigen universal for pyrethriods pesticide is synthesized successfully, thus providing a basis for establishment of a immunoassay method of the pyrethriods pesticide, and meeting the domestic requirement of rapid detection on the pyrethriods pesticide.

The invention discloses a preparation method of heavy metal cadmium artificial antigen, which applies 2-S-(4- aminobenzene)-1, 4, 7, 10 tetraazacyclo cyclononane-1, 4, 7, 10-acetate tetrahydrate(p-NH2-Bn-DOTA, DOTA for short) as cheating agent, and couples cadmium ion chelating agent composite with carrier protein bovine serum albumin BSA or egg albumin OVA; thus the artificial antigen is prepared. The method adds the sodium borohydride processing step on the basis of traditional method, and improves the coupling efficiency and antiserum titre. The invention further discloses an application ofDOTA in preparation of the heavy metal cadmium artificial antigen.

The invention relates to the technical field of biological chemistry, and particularly discloses a preparation method of fluoroacetamide hapten and application of a monoclonal antibody. The fluoroacetamide hapten is synthesized by taking ethyl fluoroacetate and p-aminophenylacetic acid as main raw materials; the fluoroacetamide hapten and carrier protein bovine serum albumin (BSA) or keyhole limpet hemocyanin (KLH) are coupled through an active lipid method, and thus a conjugate is prepared. Through experimental verification, it shows that the prepared conjugate enables a living body to generate the antibody against fluoroacetamide, that is to say, the prepared conjugate is fluoroacetamide artificial antigen. The prepared fluoroacetamide artificial antigen is suitable for enzyme-linked immunological analysis of fluoroacetamide.

The invention relates to an acidified albumin and a preparation method thereof, and an application of the acidified albumin in inhibiting beta-amyloid protein aggregation. According to the invention, diglycolic anhydride is modified on the amino group on the surface of serum albumin, such that a product with isoelectric point of 3.8-4.3 and with diglycolic anhydride average modification number between 3 and 14.5 is obtained. The product is the acidified albumin. Diglycolic anhydride powder is added into a solution of serum albumin dissolved in deionized water and with a concentration of 1-20mg/mL; a sodium hydroxide solution is dropped in at a same time, such that the pH value of the reaction liquid is stably maintained at 6.0-7.0; a stirring reaction is carried out for 0.5-5h under room temperature; and free diglycolic anhydride is removed, such that acidified serum albumin with a stable molecular structure is obtained. According to the acidified serum albumin provided by the invention, serum albumin surface negative charge amount is improved, such that the interaction of serum albumin and Abeta is promoted, and thus Abeta aggregation is inhibited, and beta-amyloid protein aggregate cytotoxicity is reduced. The acidified albumin is used in medicines used for treating Alzheimer's disease and beta-amyloid protein aggregation-related diseases.

The invention discloses a tin/protein nano hybrid membrane and applications thereof, wherein Sn<2+> reduces protein disulfide bonds to induce protein self-assembly so as to form a tin/protein composite nano film, ie., the hybrid membrane, and the protein is lysozyme, bovine serum albumin, insulin or beta-lactoglobulin. According to the invention, the preparation method of the hybrid membrane is simple, rapid and easy to realize large-area preparation; and the hybrid membrane has good biocompatibility, no obvious toxicity to cells, good chemical stability, mechanical stability and optical transparency, and good adhesion and flexibility, can be adhered to the surfaces of various base materials, has good inhibition effect on escherichia coli, staphylococcus aureus and candida albicans, further has good catalytic performance, can be used as a photocatalytic material for photocatalytic decomposition of water to produce hydrogen under visible light irradiation, and can also be used as an electrocatalytic material for preparation of a conductive polymer membrane.

The invention relates to a virus delivery culture medium. The virus delivery culture medium contains the following components in concentration: Hank'S equilibrium liquid, 0.2-2 g/L of amino acid, 5-50% of glycerol, 0.02-0.2% of a ProClin preservative and 2-15 g/L of bovine serum albumin. The virus delivery culture medium has the beneficial effects that the virus delivery culture medium is based on the Hank's equilibrium liquid, amino acid is added as a virus protein shell protection component, glycerol is added, the protection of the complete form of a virus in a long-term low-temperature stored virus sample is facilitated, the antigen activity of a virus shell is facilitated, and the virus delivery culture medium is suitable for antigen detection and nucleic acid detection of the virus; and meanwhile, the preservative is added, so that the virus delivery culture medium is suitable for virus transportation and long-term preservation. The virus delivery culture medium has no interference on the detection of an in-vitro diagnostic reagent, avoids the abuse of antibiotics, and reduces the drug resistance risk of pathogenic bacteria.

The present invention relates to one new kind of long-acting thymulin and its preparation and medical use, as well as DNA sequence coding the protein and host cell carrying the DNA sequence. The long-acting thymulin protein includes one first area with at least 85 % sequence homology with serum albumin, one second area connected to the C-end of serum albumin and with at least 85 % sequence homology with thymulin and one third area connected to the N-end of serum albumin and with at least 85 % sequence homology with thymulin. It may have one or several joining peptides. Experiments show that the long-acting thymulin has the bioactivity similar to that of thymulin as well as long half life, and may be used in treating various kinds of immunodeficiency diseases and various kinds of adaptive diseases.

The invention provides a preparation method of human serum albumin. The preparation method comprises the following steps: inoculating pichia pastoris for producing human serum albumin into a culture medium, performing fermenting, and obtaining the human serum albumin from fermentation liquor. The culture medium is a BSM culture medium in which the concentrations of other components are reduced to1/4 at most except glycerol and pichia pastoris microelements 1, and preferably, the concentrations of other components are reduced to 1/4-1/2. The invention also provides a purification method of thehuman serum albumin. The yield of the human serum albumin prepared by the preparation method and the proportion of the human serum albumin in total protein are remarkably higher than those in the prior art, cost of a culture medium used in the preparation method is remarkably reduced, and the risk of animal-derived virus pollution is avoided, so that cost of the preparation method is remarkably reduced. According to the purification method, the recovery rate of purification is remarkably increased, and the purity of a final product is remarkably and further improved.

The invention discloses a quantitative method for a dimensional electrophoresis protein sample. The method includes the steps of: preparing a dimensional electrophoresis sample; preparing a 1-2mg / mL bovine serum albumin (BSA) solution by using the a dimensional electrophoresis cell lysate, taking 8 centrifuge tubes, adding 0-200 muL of bovine serum albumin solution respectively, and supplementing ultrapure water to 1 mL to reach a final concentration of standard protein of 0-0.2mg /ml, then adding 5 mL of a 0.01% Coomassie brilliant blue G-250 or R-250 dye solution into each centrifuge tube, and fully mixing to obtain standard mixture solutions with different concentrations; preparing a dimensional electrophoresis sample mixed solution; adding the sample decrypting and framing fluorescence intensity; drawing a standard curve; and calculating the concentration of the dimensional electrophoresis protein sample. The invention has the advantages of simple operation, accuracy, fastness and cheapness.

The invention discloses a traditional Chinese medicine composition for treating chronic nephritis proteinuria and a preparation method and application thereof. The traditional Chinese medicine composition of the invention has both spleen tonifying and kidney reinforcing functions and both tonifying and astringency inducing effects, and is assisted by a small amount of dampness-excreting ingredients so as to exert spleen and kidney tonifying, qi benefiting, essence securing, pathogen eliminating, and body resistance strengthening effects. The traditional Chinese medicine composition is preparedfrom the following bulk drugs by weight: 30-50 parts of astragalus membranaceus, 20-30 parts of mantis egg-case, 15-20 parts of bighead atractylodes rhizome, 15-20 parts of semen cuscutae, 10-15 parts of schisandra chinensis, 10-15 parts of dogberry, 10-15 parts of poria cocos and 10-15 parts of honey-fried licorice roots. Clinical application proves that the traditional Chinese medicine composition can improve the clinical symptoms of patients with chronic nephritis; pharmacodynamic studies show that the composition can reduce 24-hour urine protein quantification of a cationized bovine serumalbumin (C-BSA) induced chronic nephritis model and reduce the content of serum urea nitrogen and creatinine in rats, is suitable for long-term administration of chronic nephritis patients with spleen-kidney qi deficiency syndromes, and has wide clinical application value and guiding significance.

The invention discloses a chromatographic method for separating and purifying recombinant human serum albumin-epidermal growth factor fusion protein from genetically engineered rice seeds. The methodcomprises the following specific steps: 1) extracting a crude extract containing recombinant human serum albumin-epidermal growth factor fusion from recombinant human serum albumin-epidermal growth factor fusion protein genetically engineered rice seeds; 2) performing cation exchange chromatography on the crude extract containing the human serum albumin-epidermal growth factor fusion protein to obtain a primary product I; and 3) carrying out anion exchange chromatography on the primary product I to obtain the purified human serum albumin-epidermal growth factor fusion protein target substance.According to the extraction and purification method disclosed by the invention, the recombinant human serum albumin-epidermal growth factor fusion protein of which the purity is greater than 95% canbe obtained, the protein activity is good, and the yield is high. The method is simple in process, economical, efficient and suitable for industrial production and application.

The invention provides a sample diluent for an ELISA detection kit. The sample diluent comprises serum albumin, animal IgG, a protein stabilizer, a metal ion exchanger, a salt buffer solution, Tween 20, Proclin300 and purified water. According to the invention, the protein stabilizer and the metal ion exchanger are added into the sample diluent, so that interference of a matrix effect can be effectively reduced, and non-specific adsorption can be removed; and healthy animal IgG is added, so that a to-be-detected sample can be protected, a serum environment can be simulated, interference of heterotropism antibodies and rheumatoid factors in a serum and plasma sample can be reduced, false positive is reduced, and the accuracy and repeatability of ELISA detection results are improved.

A sleep peptide fusion protein and application thereof, comprising a first region homologous to SEQ ID No: 1 serum albumin or at least 85% sequence homologous to serum albumin in the sequence table, and a sequence connected to the C-terminus of serum albumin The sleep peptide of SEQ ID No:2 in the list or the second region with at least 85% sequence homology of the sleep peptide, and the conductive peptide of SEQ ID No:3 in the sequence listing connected to the N-terminal of serum albumin or at least with the conductive peptide The third region with 85% sequence homology, the sleep peptide fusion protein can be formed by connecting the first region, the second region and the third region at the same time, or it can be composed of the first region, the second region and the third region The three regions are connected simultaneously, and the first region and the second region are formed by linking peptides; the application in the treatment of sleep disorders and insomnia.

The invention relates to a horseradish peroxidase composite gel photonic crystal sensor and a method. The horseradish peroxidase composite gel photonic crystal sensor is prepared by compounding horseradish peroxidase (HRP) for recognizing molecular protein and scaffold protein bovine serum albumin (BSA) for not interfering recognition; hRP (horseradish peroxidase) which is originally difficult to gelatinize can be cross-linked through a cross-linking agent glutaraldehyde GA to form composite protein hydrogel so as to be embedded into colloidal particles in the photonic crystal array; therefore, sensitive, label-free and visual detection of the hydrogen peroxide content in a detected object can be realized by using the gel volume (colloidal particle spacing) change caused by catalase-like reaction of horseradish peroxidase HRP and hydrogen peroxide and the Debye ring diameter change diffracted by the photonic crystal array caused by the colloidal particle spacing change.