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Bovine serum albumin nano microsphere preparation method

A technology of bovine serum albumin and nano-microspheres is applied in the field of preparing bovine serum albumin nano-microspheres based on protein dimerization, which can solve the problems of unsuitability for active protein delivery, denaturation of active protein drugs, and easy residues, etc. The effect of protecting biological activity, short treatment period and low preparation temperature

Inactive Publication Date: 2015-06-17
NANJING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the chemical cross-linking agent can make the nano-microspheres more stable in physical structure, it will cause the denaturation and inactivation of the embedded active protein drugs (such as cell growth factors, etc.), and is not suitable for the delivery of active proteins; at the same time, Chemical crosslinking agents are cytotoxic and tend to remain in the material

Method used

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  • Bovine serum albumin nano microsphere preparation method
  • Bovine serum albumin nano microsphere preparation method
  • Bovine serum albumin nano microsphere preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Prepare a sodium chloride aqueous solution with a concentration of 10mM, dissolve 100% bovine serum albumin in 5ml sodium chloride solution, see the schematic diagram for the preparation figure 1 , the relationship between the concentration of bovine serum albumin and the diameter of nanospheres is shown in figure 2 ;

[0041] (2) Dissolve 4 mg of double-ended maleimidated polyethylene glycol in 1 ml of sodium chloride solution, add it dropwise into the bovine serum albumin solution under the protection of argon, and stir at room temperature for 2 hours. The preparation diagram is shown in figure 1 , the relationship between the concentration of double-ended maleimidated polyethylene glycol and the diameter of nanospheres is shown in image 3 ;

[0042] (3) Add dropwise sodium hydroxide solution with a concentration of 1N under stirring at room temperature to adjust the pH value of the mixed solution to 7. See the relationship between the pH value and the diamete...

Embodiment 2

[0048] (1) Prepare a sodium chloride aqueous solution with a concentration of 10mM, and dissolve 150mg bovine serum albumin in 5ml sodium chloride solution;

[0049] (2) Dissolve 5 mg of double-ended maleimidated polyethylene glycol in 1 ml of sodium chloride solution, add dropwise to the bovine serum albumin solution under argon protection, and stir at room temperature for 2 hours;

[0050] (3) Add dropwise sodium hydroxide solution with a concentration of 1N under stirring at room temperature, and adjust the pH value of the mixed solution to 7;

[0051] (4) Add 6 times the volume of acetone or ethanol dropwise with stirring at room temperature until the solution is cloudy;

[0052] (5) High-speed centrifugation to obtain nanospheres, followed by washing with distilled water and absolute ethanol respectively, and finally freeze-drying to obtain bovine serum albumin nanospheres.

[0053]

Embodiment 3

[0055] (1) Prepare a sodium chloride aqueous solution with a concentration of 10mM, and dissolve 150mg bovine serum albumin in 5ml sodium chloride solution;

[0056] (2) Dissolve 6 mg of double-ended maleimidated polyethylene glycol in 1 ml of sodium chloride solution, add dropwise to the bovine serum albumin solution under the protection of argon, and stir at room temperature for 3 hours;

[0057] (3) Add dropwise sodium hydroxide solution with a concentration of 1N under stirring at room temperature, and adjust the pH value of the mixed solution to 8;

[0058] (4) Add 7 times the volume of acetone or ethanol dropwise with stirring at room temperature until the solution is cloudy;

[0059] (5) High-speed centrifugation to obtain nanospheres, followed by washing with distilled water and absolute ethanol respectively, and finally freeze-drying to obtain bovine serum albumin nanospheres.

[0060]

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Abstract

The invention discloses a bovine serum albumin nano microsphere preparation method, and provides a protein nano microsphere cross-linking method based on protein dimerization. According to the method, bovine serum albumin is used as a matrix material, and through the protein dimerization and inverse solution method, bovine serum albumin nano microsphere cross-linking can be realized to obtain a nano microsphere drug delivery material with structural stability. The method avoids the use of a toxic chemical crosslinking agent, and guarantees the use safety of the delivery system, and biological activity of the entrapped drugs is expected to retain, so that the the nano microsphere system biocompatibility can be significantly improved. The method is simple and easy in process and equipment and safe to operate, has the advantages of low preparation temperature, short processing cycle and the like, and has the application value in the fields of embedding and transfering of active drugs, proteins or gene.

Description

technical field [0001] The invention belongs to the field of biomedical polymer materials, and in particular relates to a method for preparing bovine serum albumin nano-microspheres based on protein dimerization. Background technique [0002] Nano-microsphere materials based on natural polymers have excellent biocompatibility and adjustable biodegradability, and can be used for the embedding and release of drugs, proteins or genes, and have been widely used in drug controlled release, tissue engineering and regeneration Research in fields such as medicine. Usually, drugs are metabolized quickly in the body, and frequent injections will bring great pain to patients. Selecting an appropriate nano drug-carrying system can realize the slow release of drugs in the body and achieve the desired therapeutic effect. In the field of tissue engineering and regenerative medicine, nanospheres are often used to embed cell growth factors and genes to improve the biological activity of ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/12C08J3/24C08L89/00A61K47/42
Inventor 谈华平肖超孙进晨高欣
Owner NANJING UNIV OF SCI & TECH
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