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Semen liquefier, its preparing method and semen liquefying method

A technology for liquefying agent and semen, applied in the field of in vitro diagnostic reagents, can solve the problems of inconvenient use of dry powder reagents, difficult to widely use clinically, unstable and the like, and achieve the effects of easy and wide application, good stability and simple operation.

Inactive Publication Date: 2005-02-23
深圳华康生物医学工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of the enzymatic digestion method are: the dry powder reagent is inconvenient to use, and it is difficult to be widely used in clinical practice; the protein injection is in powder form and needs to be dissolved before use, but it is extremely unstable after dissolution
The mechanical mixing method is to destroy the reticular fibers through mechanical vibration to achieve the effect of promoting liquefaction, but this method is very easy to destroy the sperm or other cells in the semen, and at the same time cause the release of sperm or intracellular substances to the seminal plasma, causing unpredictable effects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1, a semen liquefaction agent is an enzyme preservation solution containing bromelain 2-10 mg / ml, and the enzyme preservation solution is 10 g / L of sucrose, 1.755 g / L of NaCl, 22 g / L of glycine, 2 g / L bovine serum albumin (BSA) pH 7.4 in 0.2M / L phosphate (PB) buffer.

[0016] The preparation method of above-mentioned semen liquefaction agent, comprises the steps:

[0017] 1) Weigh 1.0 grams of sucrose, 0.1755 grams of NaCl, 2.2 grams of glycine, and 0.2 grams of BSA, and place them in a 100ml volumetric flask;

[0018] 2) Add 95ml of pH7.4 20mM PB buffer solution to fully dissolve the substances described in step 1) in a 100ml volumetric flask. Measure the pH of the solution with a pH meter, and correct the pH to 7.4 with NaOH or HCl. Finally, add PB buffer solution with pH 7.420mM to a total volume of 100ml to prepare an enzyme preservation solution and store it at 4°C;

[0019] 3) Weigh 10 to 50 mg of carbodiimide and dissolve it in 10 ml of 0.02M PB bu...

Embodiment 2

[0026] Embodiment 2, a semen liquefaction agent is an enzyme preservation solution containing 2-10 g / l of bromelain, and the enzyme preservation solution is glucose containing 5-15 g / L, NaCl of 1-5 g / L, 0.5-1.5 g / L L gelatin, 1~5g / L BSA, pH7.4 0.2M / L PB buffer.

[0027] The preparation method of above-mentioned semen liquefaction agent, comprises the steps:

[0028] 1) Weigh 0.5-1.5 grams of glucose, 0.1-0.5 grams of NaCl, 0.05-0.15 grams of gelatin, and 0.1-0.5 grams of BSA, and place them in a 100ml volumetric flask;

[0029] 2) Add 95ml pH7.4 15-50mM PB buffer solution to fully dissolve the substance in step 1) in a 100ml volumetric flask. Measure the pH of the solution with a pH meter, and correct the pH to 7.4 with NaOH or HCl. Finally, add PB buffer solution with a pH of 7.415-25mM to a total volume of 100ml to prepare an enzyme preservation solution and store it at 4°C;

[0030] 3) Weigh 10-50mg of carbodiimide and dissolve it in 10ml of 0.02M PB buffer solution, add...

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Abstract

A sperm liquefying agent is an enzyme preserving fluid. Its preparing method includes adding weighed protein hydrlytic enzyme and carbodiimide into enzyme preserving fluid for reaction of 4-12 hour at 4 degree C, adding 2-aminothanol for reaction of 1-4 hour at room temperature, adding regulatig fluid concenration to be 2-10g / l. A liquefying method is also disclosed.

Description

technical field [0001] The invention relates to an in vitro diagnostic reagent, which is particularly suitable for the liquefaction of abnormal semen. Background technique [0002] Abnormally liquefied semen samples are too viscous. When performing immunobiological and chemical tests, it is difficult to mix and add samples accurately due to the uneven distribution of the substances to be tested in the semen, and it is often difficult to obtain accurate test results. Therefore, adding liquefaction agents to digest semen The detection of specimens is an essential pretreatment procedure for further detection and analysis of liquefied abnormal semen. [0003] Commonly used methods for processing non-liquefied samples include enzymatic digestion and mechanical mixing. The enzymatic digestion method mainly uses protease dry powder reagent or protease injection to hydrolyze the fibrin in the semen to liquefy the semen. The disadvantages of the enzymatic digestion method are: the ...

Claims

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Application Information

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IPC IPC(8): G01N33/531
Inventor 傅剑华刘瑜胡家纯何林何小红
Owner 深圳华康生物医学工程有限公司
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