Tin/protein nano hybrid membrane and applications thereof

A protein and hybrid membrane technology, applied in the field of multifunctional tin-doped two-dimensional protein nanomembranes, can solve the problems of difficulty and low efficiency of metal/protein hybrid materials, and achieve good chemical stability and mechanical stability. , the preparation method is simple, the effect of good catalytic performance

Active Publication Date: 2020-04-10
SHAANXI NORMAL UNIV
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This limitation often results in the difficulty and inefficien

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tin/protein nano hybrid membrane and applications thereof
  • Tin/protein nano hybrid membrane and applications thereof
  • Tin/protein nano hybrid membrane and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] At room temperature, add 100mg of lysozyme to a 10mL Petri dish filled with 10mL of 10mmol / L pH 7.4 4-hydroxyethylpiperazineethanesulfonic acid buffer solution, and then add 0.08g of SnCl 2 , mix evenly, and the pH of the resulting mixed solution is adjusted to be 1.8 with hydrochloric acid, and a layer of tin / lysozyme nano-hybrid film is clearly floating on the gas-liquid interface within 5min (see Figure 1~3 ). The obtained tin / lysozyme nano-hybrid film has a thickness of 50 nm, good flexibility, Young's modulus of elasticity of 0.2 GPa, and hardness of 0.02 GPa.

Embodiment 2

[0027] At room temperature, add 200mg of lysozyme to a 10mL Petri dish filled with 10mL of 10mmol / L pH 7.4 4-hydroxyethylpiperazineethanesulfonic acid buffer solution, and then add 0.08g of SnCl 2 , mixed evenly, the pH of the resulting mixture was adjusted to 1.8 with hydrochloric acid, and a tin / lysozyme nano-hybrid film was clearly formed at the gas-liquid interface within 5 minutes.

Embodiment 3

[0029] At room temperature, add 50mg of lysozyme to a 10mL Petri dish filled with 10mL of 10mmol / L pH 7.4 4-hydroxyethylpiperazineethanesulfonic acid buffer solution, and then add 0.08g of SnCl 2 , mixed evenly, the pH of the resulting mixture was adjusted to 1.8 with hydrochloric acid, and a tin / lysozyme nano-hybrid film was clearly formed at the gas-liquid interface within 5 minutes.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Thicknessaaaaaaaaaa
Young's modulus of elasticityaaaaaaaaaa
Hardnessaaaaaaaaaa
Login to view more

Abstract

The invention discloses a tin/protein nano hybrid membrane and applications thereof, wherein Sn<2+> reduces protein disulfide bonds to induce protein self-assembly so as to form a tin/protein composite nano film, ie., the hybrid membrane, and the protein is lysozyme, bovine serum albumin, insulin or beta-lactoglobulin. According to the invention, the preparation method of the hybrid membrane is simple, rapid and easy to realize large-area preparation; and the hybrid membrane has good biocompatibility, no obvious toxicity to cells, good chemical stability, mechanical stability and optical transparency, and good adhesion and flexibility, can be adhered to the surfaces of various base materials, has good inhibition effect on escherichia coli, staphylococcus aureus and candida albicans, further has good catalytic performance, can be used as a photocatalytic material for photocatalytic decomposition of water to produce hydrogen under visible light irradiation, and can also be used as an electrocatalytic material for preparation of a conductive polymer membrane.

Description

technical field [0001] The present invention relates to a multifunctional tin-doped two-dimensional protein nanofilm, and multiple uses of the film in micropatterning, antibacterial and photo / electrocatalysis. Background technique [0002] The self-assembly of peptides and proteins to create structurally defined and stable two-dimensional (2D) nanoscale assemblies has important biological and technological implications. Proteins have greater structural and chemical complexity than peptide-based systems, and thus, the assembly of proteins into supramolecular structures is often non-trivial and more challenging than the process for peptides. In particular, its assembly into a macroscopic protein 2D material with a larger scale and uniformity, compared with the general large-scale natural objects, remains a challenge. Furthermore, low assembly efficiency and speed hinder scalable production. [0003] Metal ions play a vital role in more than one-third of natural proteins in n...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C08J5/18C08L89/00C08K3/16G03F7/004C08G73/06A01N59/16A01N37/46A01P1/00A01P3/00B01J31/06C25B11/06C25B1/04C01B3/04
CPCC08J5/18G03F7/004C08G73/0611A01N59/16A01N37/46B01J31/069B01J35/004B01J35/0033C25B1/04C01B3/042C08J2389/00C08K3/16C25B1/55C25B11/095Y02E60/36
Inventor 杨鹏巴萨姆·赛义夫
Owner SHAANXI NORMAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap