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Virus delivery culture medium

A culture medium and virus technology, applied in the field of virus culture medium, can solve the problems of easy breeding of bacteria, short storage time of virus preservation reagents, virus destruction, etc., to reduce the risk of drug resistance of pathogenic bacteria, superior compatibility and stability, Beneficial effect on antigenic activity

Pending Publication Date: 2021-05-11
苏州赛普生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems in the prior art that the storage time of virus preservation reagents is short, the virus is easily destroyed, and bacteria are easy to breed, the present invention provides a virus transport medium

Method used

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Examples

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Effect test

Embodiment 1

[0055] An embodiment of the present invention provides a virus delivery medium, the virus delivery medium contains components at the following concentrations:

[0056] Hank'S balance solution, amino acid 1.3g / L, glycerin 20% (v / v), ProClin preservative 0.11% (v / v), bovine serum albumin 5g / L, phenol red 0.1g / L.

[0057] In the Hank'S balance solution, the following concentration components are included:

[0058] 4-Hydroxyethylpiperazineethanesulfonic acid 5g / L, NaCl: 8.22g / L, KCl: 2g / L, glucose 1g / L, disodium hydrogen phosphate 0.05g / L, CaCl 2 : 0.06g / L, MgCl 2 : 0.05g / L.

[0059] Contain the components of following concentration in the described ProClin antiseptic:

[0060] ProClin300 with a volume fraction of 0.1%, and ProClin500 with a volume fraction of 0.01%.

[0061] In addition, an embodiment of the present invention also provides a method for preparing the above-mentioned virus delivery medium, including the following steps:

[0062] Take solid state 4-hydroxyethyl...

Embodiment 2

[0064] In the embodiment of the present invention, the antigen detection performance verification of the virus delivery medium in Example 1 is carried out, specifically,

[0065] Take 9 parts of the virus delivery medium of the concentration components in Example 1, add the same concentration of lentivirus to each copy of the virus delivery medium, divide it into three groups of A, B, and C, and each group has 3 copies, and each copy of the virus delivery medium can be For testing more than 6 times. Groups A, B, and C were stored under different temperature conditions for virus delivery media. Group A was stored at room temperature, group B was stored at -20°C, and group C was stored at -80°C. Lentivirus detection was performed on day 0, day 2, day 3, day 7, day 14, and day 30, respectively. The lentivirus titer ELISA detection kit was used for detection, the OD value was measured by a microplate reader, the average value was taken, and the lentivirus titer was calculated usi...

Embodiment 3

[0072] This embodiment verifies the nucleic acid detection performance of the virus transport medium of Example 1. Specifically, take 10 parts of the virus transport medium (C) of Example 1 of the present invention, add RNA pseudovirus, so that the concentration of each sample is 2000copies / mL. The culture medium samples added with RNA pseudoviruses were placed at room temperature for storage, and nucleic acid extraction and qPCR detection were performed on the 0th, 1st, 3rd, 7th, and 14th days of storage respectively.

[0073] The test results are shown in Table 2:

[0074] Table 2 Example 1 The qPCR detection results of the virus delivery medium at different times

[0075]

[0076] Analysis of the experimental results in Table 2 shows that the RNA pseudoviruses preserved in the virus delivery medium of Example 1 of the present invention were stored at room temperature for 3 days, and the CT value had no significant change, and were stored at room temperature for one wee...

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Abstract

The invention relates to a virus delivery culture medium. The virus delivery culture medium contains the following components in concentration: Hank'S equilibrium liquid, 0.2-2 g / L of amino acid, 5-50% of glycerol, 0.02-0.2% of a ProClin preservative and 2-15 g / L of bovine serum albumin. The virus delivery culture medium has the beneficial effects that the virus delivery culture medium is based on the Hank's equilibrium liquid, amino acid is added as a virus protein shell protection component, glycerol is added, the protection of the complete form of a virus in a long-term low-temperature stored virus sample is facilitated, the antigen activity of a virus shell is facilitated, and the virus delivery culture medium is suitable for antigen detection and nucleic acid detection of the virus; and meanwhile, the preservative is added, so that the virus delivery culture medium is suitable for virus transportation and long-term preservation. The virus delivery culture medium has no interference on the detection of an in-vitro diagnostic reagent, avoids the abuse of antibiotics, and reduces the drug resistance risk of pathogenic bacteria.

Description

technical field [0001] The invention belongs to the technical field of virus culture medium, and in particular relates to a virus transport culture medium. Background technique [0002] Viruses are simple in structure, generally composed of nucleic acid (DNA or RNA) and a protein shell that wraps nucleic acid. They are non-cellular life forms that must parasiticly proliferate in living cells. Most viruses are pathogenic microorganisms that can cause disease in the host and can be transmitted between organisms to be infectious. In the prior art, in vitro diagnosis can be performed by collecting samples from patients to detect whether they are infected with a certain virus. The common detection method is to perform antigen antibody detection or nucleic acid detection on patient samples, but the virus will generally be inactivated soon after leaving the host cell, the integrity of the virus will be destroyed, the virus shell will disintegrate, and the nucleic acid will be degr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04C12R1/93
CPCC12N1/04
Inventor 张胜有叶竹青
Owner 苏州赛普生物科技股份有限公司
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