25 results about "Antibiotic misuse" patented technology

The invention relates to the field of medical detection, in particular to a fluorescent sensing composition, a fluorescent sensing film and application thereof. According to the invention, by screening a plurality of fluorescent indicators and combinations thereof, an indicator formula with simple components and low cost is finally obtained. Based on the formula, by optimizing the preparation process, the high-efficiency fluorescent sensing film resistant to damp-heat sterilization is developed. After a microorganism culture bottle prepared on the basis of the membrane is inoculated with Gram-negative bacilli, positive cocci and yeast fungi representative quality control strains, experiments of full-automatic microorganism culture system fluorescence curve determination, Gram staining, microorganism mass spectrum identification and the like prove that the fluorescence sensing membrane is excellent in detection performance. The fluorescent sensing film provided by the research is applied to clinical microorganism detection, can provide technical support for blood flow infection diagnosis, and is of great significance in suppressing antibiotic abuse and improving prognosis of blood flow infection patients.

The invention discloses pediococcus pentosaceus TRM54998 with broad-spectrum antibacterial activity and application of the pediococcus pentosaceus TRM54998, and belongs to the field of microorganisms. The strain is preserved in the China Center for Type Culture Collection (CCTCC) on June 04, 2024, and the preservation number is CCTCC NO: M 20241142. It is proved that the strain has good acid resistance and bile salt resistance and can grow in the environment with the bovine bile salt concentration being 0.1-0.2% (w / v) or in the pH value range being 3-7; the fermentation supernatant has an inhibition effect on nine pathogenic bacteria such as salmonella and the like, and the antibacterial effect is stable; meanwhile, experiments prove that the pediococcus pentosaceus TRM54998 has no wide drug resistance, can relieve the problems of aggravated drug resistance, damage to microbial ecological balance and the like caused by antibiotic abuse, provides a strain resource for preparing a biological bacteriostatic agent or an intestinal probiotic agent, and has a wide application prospect.

The invention discloses application of triptolide ketone in preparation of a medicine for treating sepsis lung injury, and belongs to the technical field of biological medicine. Aiming at the problems that traditional chemical synthetic drugs may cause a large amount of sequelae and antibiotic abuse, and triptolide may cause liver and kidney injury when treating sepsis, research finds that triptolide can be used for treating sepsis lung injury, and can be used for treating sepsis lung injury. According to the application, the protein expression level of HIF-1alpha in proinflammatory activated macrophages is lowered, so that aerobic glycolysis is inhibited, and the effect of treating sepsis lung injury is achieved. Compared with the triptolide alcohol, the triptolide ketone does not cause liver and kidney injury while playing a role in treating the sepsis lung injury, and the curative effect of the triptolide ketone is equivalent to that of the existing medicine 2ME2 for treating the sepsis lung injury, so that the triptolide ketone has important application potential clinically, and can be used for preparing medicines for treating the sepsis lung injury. And a theoretical basis is provided for clinically developing medicines for treating sepsis lung injury.

The invention relates to the technical field of microorganisms, in particular to a probiotic product for inhibiting salmonella in a chicken body and a screening method, the probiotic product comprises Chencity bacillus sachariensis, and the colony count of the Chencity bacillus sachariensis in the probiotic product is 107-1010 CFU / mL. Based on the screening method of the probiotics for inhibiting the salmonella in the chicken body, the screened city bacillus sachariensis is prepared into the probiotic product for inhibiting the salmonella in the chicken body, so that the salmonella infection condition of the chicken can be effectively improved, the weight of the chicken with positive salmonella infection is increased, and the salmonella in the chicken body is inhibited. The salmonella loading quantity in chicken bodies is obviously reduced, the salmonella loading quantity has the characteristics of low residual quantity and environment friendliness, and the problems of antibiotic residues, bacterial drug resistance increase and the like caused by antibiotic abuse can be avoided. By adopting the screening method of the probiotics for inhibiting the salmonella in the chicken body, provided by the invention, the dominant probiotics capable of inhibiting the salmonella can be simply, conveniently and efficiently determined.

A new antibacterial peptide of Japanese eel A1 Cath2b 143‑174 and its application, belong to the field of biotechnology. The antibacterial peptide is a Cathelicidin family antibacterial peptide of Japanese eel A1 The 143-174 amino acid fragment of Cathelicidin 2b is named as Cath2b A1 Cath2b 143‑174 , and the amino acid sequence is shown as SEQ ID NO. 3. The antibacterial peptide has high inhibition / killing activity on various aquatic pathogenic bacteria, has excellent thermal stability and salt ion tolerance, and has no obvious toxicity to host cells; it plays a bactericidal role by destroying the bacterial cell membrane, and can also attract host immune cells to enhance immunity. The antibacterial peptide can be applied in the preparation of aquatic animal bacterial disease prevention and control drugs. It can solve the problems of drug resistance and drug residue caused by the abuse of traditional antibiotics, and is convenient to prepare, has strong adaptability, and is suitable for the prevention and control of bacterial diseases of aquatic animals.

The invention discloses a strain of leuconostoc mesenteroides V7, and the microbial preservation number is CGMCC (China General Microbiological Culture Collection Center) No.34824. The leuconostoc mesenteroides V7 disclosed by the invention is applied to products for inhibiting source avian pathogenic escherichia coli. The leuconostoc mesenteroides V7 disclosed by the invention has an inhibition effect on avian pathogenic escherichia coli, so that the leuconostoc mesenteroides V7 can be used for replacing antibiotics in poultry production and breeding to treat avian pathogenic colibacillosis. The invention provides a novel antibiotic substitute, which can obviously improve the infection condition of avian pathogenic escherichia coli in poultry bodies and avoid the problems of antibiotic abuse and bacterial drug resistance as much as possible.

Marine pollution caused by antibiotic abuse has become a global environmental challenge, wherein trace detection of quinolone antibiotics (such as fleroxacin FLE, norfloxacin NOR and enrofloxacin ENR) is particularly key. The invention develops a high-stability fluorescence sensor based on a lanthanide series metal organic framework (Ln-MOF (at) BTEC), and by introducing a hydrogen bond network strengthening strategy, the performance of the material in a seawater high-salt environment is remarkably improved. The fluorescence decay rate of the material in simulated seawater within 60 min is as low as 2.67%, which is 21.49% in 30 min compared with that of a base material, and the stability is obviously improved. In addition, the ratio proportional fluorescence sensing can effectively and instantaneously respond to FLE, NOR and ENR in the presence of numerous interferents in a seawater system, and the detection limits within the range of 0-10 [mu] M are 80.7 nM, 21.03 nM and 28.22 nM respectively. Meanwhile, an intelligent mobile phone platform is successfully integrated to realize visual field detection, and the standard addition recovery rate in Dalian coastal areas and seawater in culture areas reaches 83-115% (RSDlt; the research provides a novel solution with high stability and high sensitivity for marine antibiotic pollution monitoring.

The invention discloses an application of lactobacillus plantarum RT1 with an antibacterial effect. The lactobacillus plantarum RT1 disclosed by the invention has excellent acid-resistant and cholate-resistant characteristics and broad-spectrum and efficient antibacterial action, can be used as a novel efficient antibacterial probiotic candidate, and is applied to the development of food preservatives, functional foods (such as fermented milk and probiotic solid beverages), feed additives and health-care products related to intestinal health; and a new microbial resource is provided for solving the problem of intestinal flora imbalance caused by food spoilage and antibiotic abuse.

The invention relates to the technical field of microorganisms, in particular to a method for reducing the dosage of antibiotics for treating clostridium perfringens infection, a bactericidal composition, bacteriophage and application. According to the method, the clostridium perfringens bacteriophage RDP-CP-2310008 and one or more antibiotics are used in a combined mode, and the clostridium perfringens bacteriophage RDP-CP-2310008 and the one or more antibiotics are used in a combined mode. The clostridium perfringens bacteriophage RDP-CP-2310008 is preserved in the China General Microbiological Culture Collection Center on November 29, 2023, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.45778. The clostridium perfringens bacteriophage RDP-CP-2310008 can be used The bactericidal composition is prepared from the following effective components: the clostridium perfringens bacteriophage RDP-CP-2310008, and one or more antibiotics used for resisting the clostridium perfringens. The invention further discloses application of the clostridium perfringens phage RDP-CP-2310008 in preparation of a medicine for preventing and / or treating clostridium perfringens infection. The invention provides and verifies that the specific bacteriophage is combined with the antibiotics for the first time, so that the dosage of the antibiotics can be greatly reduced and can be up to 64 times, and a brand-new path is provided for solving the problem of abuse of the antibiotics in the breeding industry.

The application discloses an antagonistic bacterium of pathogenic shewanella of Apostichopus japonicus and application thereof, and belongs to the technical field of aquatic microorganisms. Gilvus japonicus The antagonistic bacterium is separated from the intestinal tract of healthy Apostichopus japonicus and is identified as Japanese Yellow Sea Oceanic Bacterium (Shewanella japonica), has no beta-hemolysis characteristic, is safe and non-toxic to Apostichopus japonicus, and can significantly antagonize the pathogenic bacterium Shewanella of Apostichopus japonicus skin rot syndrome. The application also discloses application of the antagonistic bacterium, which can be prepared into an aquatic microecological preparation for preventing and treating the skin rot syndrome of Apostichopus japonicus, and is applied through intraperitoneal injection or immersion, so that the survival rate of Apostichopus japonicus under exposure of the pathogenic bacterium is significantly improved. The strain has strong salt and alkali resistance, tolerances to various antibiotics and chemical drugs, and strong environmental adaptability, can be applied to the cultivation of Apostichopus japonicus to replace traditional antibiotics, effectively solves the problems of food safety and environmental pollution caused by the abuse of antibiotics, and has good industrial application prospect.

The invention relates to an anti-biofilm peptide and application thereof, and belongs to the technical field of antibacterial peptides. The invention relates to an anti-biofilm peptide VKLR. The amino acid sequence of the anti-biofilm peptide VKLR is as shown in SEQ ID NO: 1: LVKLRVKLRVKLR. The broad-spectrum antibacterial agent aims at bacteria including gram-negative bacteria and gram-positive bacteria. The anti-biofilm peptide VKLR disclosed by the invention has broad-spectrum antibacterial activity and has a remarkable antibacterial effect on various gram-negative bacteria and gram-positive bacteria such as escherichia coli, pseudomonas aeruginosa, staphylococcus aureus and staphylococcus epidermidis, and infection caused by a pathogen biofilm seriously threatens human and animal health; in the breeding process, abuse of traditional antibiotics is likely to generate drug resistance risks, and people pay attention to novel antibacterial drug substitutes. According to the present invention, the 14-element library of the AMPs is obtained, the peptide VKLR shows the huge potential in the antibacterial effect aspect, and is the ideal candidate for treating bacterial infection, such that the research provides the new insight for the future design of the biofilm removing peptide, and the wide application of the biofilm removing peptide in the medicine and the animal husbandry is promoted.

The invention discloses application of thiamine in improving the sugar utilization rate and growth performance of sturgeons, and belongs to the field of aquaculture. It is proved for the first time that by enhancing a glycolytic pathway, inhibiting a gluconeogenesis pathway and promoting glycogen decomposition, the thiamine effectively improves the sugar metabolism capacity of sturgeons and reduces hepatic glycogen deposition and fat accumulation, so that the risk of metabolic disorder is reduced. Meanwhile, thiamine also promotes muscle fiber growth, so that the growth speed of the sturgeons and the feed utilization rate are remarkably increased. Therefore, the thiamine can be used as a feed additive for preparing the feed for improving the sugar utilization rate and the growth performance of the sturgeons. A safe and efficient nutrition regulation and control scheme is provided for sturgeon culture, the feed utilization rate and culture benefits are improved, meanwhile, ecological risks such as antibiotic abuse are avoided, and remarkable economic benefits and application prospects are achieved.

The invention discloses a health-care pig feed containing Chinese herbal medicines and a preparation method thereof, the health-care pig feed comprises a basal feed and a Chinese herbal medicine composition, and the Chinese herbal medicine composition is prepared from the following raw materials in parts by weight: 10-20 parts of astragalus membranaceus, 5-10 parts of liquorice, 5-10 parts of honeysuckle, 10-20 parts of hawthorn, 10-15 parts of malt, 10-15 parts of medicated leaven, 5-10 parts of pericarpium citri reticulatae and 10-15 parts of poria cocos; the total weight of the Chinese herbal medicine composition accounts for 5-15% of the total weight of the health-care pig feed. The invention belongs to the technical field of feeds, and particularly provides a pig feed for solving the problems of low immunity caused by single nutrition of a traditional pig feed and drug residues and bacterial drug resistance caused by abuse of antibiotics.

The invention discloses application of parabacteroides dieldrii in preparation of a medicine for treating prostatic cancer, and belongs to the field of biological medicine. It is found for the first time that the abundance of parabacteroides dieldrii in intestinal flora of a prostate cancer patient is remarkably reduced, and the parabacteroides dieldrii is selected as potential probiotics for inhibiting growth of prostate cancer tumors. The result of a parabacteroides dieldrii single bacterium colonization experiment shows that the parabacteroides dieldrii and the metabolite gamma-linolenic acid (GLA) thereof can efficiently inhibit the growth of the prostatic cancer tumor, can also improve the condition that the treatment effect of docetaxel on the prostatic cancer is poor due to abuse of clinical antibiotics, and can be prepared into the medicine for treating or assisting in treating the prostatic cancer. The invention provides a new treatment strategy and medicine for the treatment of the prostatic cancer, and also provides a brand new medicine development path and a clinical application scheme for the micro-ecological treatment of the prostatic cancer.

The application provides a high positive charge self-assembled ruthenium complex targeting electron transport chain and a preparation method and application thereof, and belongs to the technical field of biological medicines.The ruthenium complex provided by the application has a structural formula I shown in formula I; wherein 1<=n<=15.The high positive charge self-assembled ruthenium complex targeting electron transport chain is synthesized for the first time, the complex shows high-efficiency anti-MRSA activity, and can eliminate bacteria without toxic side effects, thereby showing good high-efficiency low-toxicity antibacterial activity, and providing a novel high-efficiency high-biocompatibility antibacterial strategy for solving problems such as antibiotic abuse and antibiotic resistance of bacteria.

The present invention relates to an antimicrobial adjuvant containing a biphenyl derivative compound as an active ingredient, and a technology of various uses thereof. The compound of the present invention reduces the dosage of polymyxin antibiotics, which are administered to inhibit the proliferation of gram-negative bacteria, by enhancing the sensitivity of the gram-negative bacteria with respect to the polymyxin antibiotics, is concomitantly administered with the polymyxin antibiotics to show gram-negative bacteria growth inhibitory and killing effects, may notably reduce side effects such as nephrotoxicity, and may prevent or treat sepsis and septic shock due to antibiotic overuse.

The feed is prepared from more than 100 Chinese herbal medicines such as herba ephedrae, cassia twigs, radix astragali, honeysuckle flowers and the like, and the Chinese herbal medicines take effect synergistically according to a specific ratio. The preparation method comprises the following steps: cleaning the medicinal materials, cooking at 100 DEG C for 30 minutes, drying until the water content is 12-14%, crushing, stirring and mixing, granulating and sterilizing. The invention belongs to the technical field of feeds, and particularly provides a feed for solving the problems of low immunity caused by single nutrition of a traditional sheep feed, drug residue and bacterial drug resistance caused by abuse of antibiotics, insufficient efficacy of an existing Chinese herbal medicine feed and the like.

This invention belongs to the field of shrimp farming technology, specifically relating to a method for raising Litopenaeus vannamei larvae using bioflocs. The method includes the following steps: (1) preparing raceway-shaped bioflocs; (2) preparing functional bioflocs; and (3) raising mysids and larvae; and raising adult larvae. This invention provides an effective method for raising Litopenaeus vannamei larvae without the use of antibiotics, solving the problem of antibiotic abuse during the larval stage, and is of great significance for promoting the green and healthy development of the shrimp larval industry.

The present application relates to the field of medical detection, in particular to a fluorescence sensing composition, a fluorescence sensing film and application thereof. Through screening a variety of fluorescence indicators and combinations thereof, a simple and cost-effective indicator formula is finally obtained. Based on the formula, through optimizing the preparation process, a high-efficiency fluorescence sensing film resistant to moist heat sterilization is developed. After inoculating gram-negative bacilli, gram-positive cocci and yeast-type fungi representative quality control strains into the microorganism culture bottle prepared based on the film, through fluorescence curve determination of the full-automatic microorganism culture system, gram staining, microorganism mass spectrum identification and other experiments, it is proved that the fluorescence sensing film has excellent detection performance. The fluorescence sensing film proposed in the present application can be applied to the detection of clinical microorganisms, which can provide technical support for the diagnosis of bloodstream infections, and has important significance for curbing the abuse of antibiotics and improving the prognosis of patients with bloodstream infections.

This application discloses an intelligent monitoring method, electronic device, and medium for porcine reproductive and respiratory syndrome (PRRS) based on multimodal perception and semantic retrieval. The method includes: real-time acquisition and preprocessing of multimodal data from pigs in a pigsty; construction of a PRRS knowledge graph; vectorization of data for different modalities to obtain query vectors; filtering candidate rules based on keyword matching; calculating a comprehensive score based on the candidate rules; sorting the comprehensive scores in descending order and outputting the top K rule texts; generating initial results based on a large language model; validating the initial results based on logical rules in the knowledge graph; and outputting the results after successful validation. This invention employs multimodal data fusion and a RAG architecture, combined with multi-sensor methods to capture early symptoms of PRRS, achieving standardized monitoring and reducing misdiagnosis and antibiotic overuse. It also supports dynamic updates of the knowledge base, full-process offline deployment to ensure data security, and rule validation to reduce medication risks.

The invention discloses an Arteratt lactobacillus TRM54999 strain with broad-spectrum antibacterial activity and application of the Arteratt lactobacillus TRM54999 strain, and belongs to the field of microorganisms. The strain is preserved in the China Center for Type Culture Collection (CCTCC) on June 4, 2024, and the preservation number is CCTCC NO: M 20241143. It is proved that the strain has good acid resistance and bile salt resistance and can grow in the environment with the bovine bile salt concentration being 1-2% (w / v) or in the pH value range being 3-7; the fermentation supernatant has an inhibition effect on nine pathogenic bacteria such as salmonella and the like, and the antibacterial effect is stable; meanwhile, experiments prove that the Agentlat lactobacillus TRM54999 has no wide drug resistance and can relieve the problems of aggravated drug resistance, damage to microbial ecological balance and the like caused by antibiotic abuse, and the strain provides a strain resource for preparing a biological bacteriostatic agent or an intestinal probiotic agent and has a wide application prospect.

The present application relates to blood culture bottle technical field, especially to composition and its application in the preparation of microbial culture medium, the present application combines yeast extract powder, soybean peptone and pancreatic casein peptone into nitrogen source, forming aerobic and anaerobic culture medium for clinical pathogenic microorganism culture, through comparison and verification, the aerobic and anaerobic culture medium added with the composition as described above is superior to prior art and other formula in culture speed and biomass accumulation in the growth experiment of 9 aerobic bacteria and 7 anaerobic bacteria, which shows that the composition as described above can effectively promote the performance of blood culture product, improve the ability of clinical blood stream infection detection, curb antibiotic abuse and save patients' lives.

The invention relates to an application of L-tryptophan in reversing T-2 toxin induced escherichia coli pan-drug resistance. T-2 toxin can induce Escherichia coli ATCC 25922 under the concentration of 10 ng / mL to generate pan-drug resistance, and failure to various antibiotics is difficult to deal with by the existing means. According to the invention, the L-tryptophan is exogenously added, so that the sensitivity of T-2 toxin induced pan-drug-resistant escherichia coli to beta-lactam antibiotics can be specifically recovered. The L-tryptophan is exogenously added, so that the content of bacterial biofilms can be reduced, and the sensitivity of bacteria to antibiotics can be improved. L-tryptophan is natural amino acid and is high in biocompatibility and low in toxicity, a foundation is laid for control of drug-resistant bacteria and reasonable use of antibiotics in a T-2 toxin pollution scene, drug-resistant bacteria transmission and antibiotic abuse can be reduced, higher safety and operability are achieved, and great significance is achieved on treatment of drug-resistant infection.

The application discloses a one-step crosslinking type photodynamic high-antibacterial hydrogel and a preparation method thereof. The hydrogel takes polydopamine, hydrolyzed keratin and polyacrylamide as main raw materials, uses tetramethyl ethylenediamine and potassium persulfate as crosslinking agents, and combines the one-step crosslinking method to prepare a multifunctional composite hydrogel with high antibacterial activity and wound healing promotion, so as to provide a new type of antibacterial hydrogel medical dressing for wound healing promotion by enhancing the mechanical property, adhesion, biocompatibility and photothermal antibacterial property of the hydrogel. The polydopamine prepared from mussels endows the hydrogel with the photothermal antibacterial property, and provides a new antibacterial strategy for the enhanced drug resistance of pathogenic bacteria caused by antibiotic abuse. The keratin is used as the raw material, so that the application reduces the environmental hazards of waste keratin, promotes the development and application of keratin in the biological medicine field, promotes the in-depth excavation of keratin resources, and realizes the high utilization of waste resources.

The invention relates to the technical field of microorganisms, and provides a bacteriophage perforin HolKpV318 with a broad-spectrum antibacterial effect and application of the bacteriophage perforin HolKpV318. The invention provides a potential novel antibacterial strategy aiming at the clinical problem of increasingly severe multi-drug-resistant klebsiella pneumoniae infection caused by antibiotic abuse at present, so as to overcome the defect that the traditional antibiotic treatment is invalid or poor in effect; the invention provides the perforin protein (HolKpV318) with efficient and specific klebsiella pneumoniae splitting capability, and the protein can form a pore channel on a klebsiella pneumoniae cell membrane to destroy the integrity of a bacterial structure so as to cause the death of target bacteria, so that a brand new biological preparation choice is provided for solving the problem of drug-resistant bacterium infection.