The invention belongs to the technical field of feeds, and discloses a livestock and poultry fresh feed produced from a variety of dregs and a variety of bacteria by secondary fermentation and a preparation process thereof. The main technical characteristic of the invention is that the livestock and poultry fresh feed consists of 10 to 50 parts by weight of water, 4 to 6 parts by weight of soybean sauce dregs, 10 to 30 parts by weight of distilled spirit dregs, 5 to 15 parts by weight of beer dregs, 4 to 6 parts by weight of wine dregs, 5 to 15 parts by weight of wheat bran, 10 to 30 parts by weight of cottonseed meal, 5 to 15 parts by weight of soybean meal, 5 to 15 parts by weight of rapeseed meal, 5 to 15 parts by weight of peanut meal, 0.2 to 0.5 parts by weight of a variety of bacteria including bacillus subtilis, lactobacillus, saccharomycetes, aspergillus niger and chitosan oligosaccharide and 0.02 to 0.05 parts by weight of enzymic preparations. The livestock and poultry fresh feed solves the problems existing in the livestock and poultry breeding industry that food shortage caused by the contention between animals and human being for food and antibiotic misuse seriously threatening the health and life style of human being, and also solves the problems on how to open outlets for agricultural and sideline products and scientifically breed livestock and poultry, the livestock and poultry fresh feed is convenient to store for a long time, the feeding effect is good, and the cost is low.