77 results about "Antibiotic misuse" patented technology

Antibiotics (Abx) are the world's most misused drugs. Antibiotics misuse occurs when the drug is administered in case of a non-bacterial infection (such as a viral infection) for which it is ineffective. Overall, it is estimated that 40-70% of the worldwide Abx courses are mis-prescribed. The financial and health consequences of Abx over-prescription include the direct cost of the drugs, as well as the indirect costs of their side effects, which are estimated at >$15 billion annually. Furthermore, over-prescription directly causes the emergence of Abx-resistant strains of bacteria, which are recognized as one of the major threats to public health today. This generates an immediate need for reliable diagnostics to assist physicians in correct Abx prescription, especially at the point-of-care (POC) where most Abx are prescribed. Accordingly, some aspects of the present invention provide methods using biomarkers for rapidly detecting the source of infection and administrating the appropriate treatment.

Antibiotics (Abx) are the worlds most misused drugs. Antibiotics misuse occurs when the drug is administered in case of a non-bacterial infection (such as a viral infection) for which it is ineffective. Overall, it is estimated that 40-70% of the worldwide Abx courses are mis-prescribed. The financial and health consequences of Abx over-prescription include the direct cost of the drugs, as well as the indirect costs of their side effects, which are estimated at >$15 billion annually. Furthermore, over-prescription directly causes the emergence of Abx-resistant strains of bacteria, which are recognized as one of the major threats to public health today. This generates an immediate need for reliable diagnostics to assist physicians in correct Abx prescription, especially at the point-of-care (POC) where most Abx are prescribed. Accordingly, some aspects of the present invention provide methods using biomarkers for rapidly detecting the source of infection and administrating the appropriate treatment.

The present invention relates to preparation of two lytic vibrio harveyi bacteriophage preparations, and an application thereof. A purpose of the present invention is to reduce the number of target pathogenic bacteria in the stichopus japonicus body, the stichopus japonicus culture environment and the stichopus japonicus processing products. Host bacteria of the bacteriophage are vibrio harveyi. The two separated bacteriophages of vp-1 and vp-2 provide strong lysis for the vibrio harveyi, and provide protection effects for the stichopus japonicus and the stichopus japonicus products. The preparation of the present invention is a novel green biological disinfection preparation. According to the present invention, the bacteriophage is purified, amplified, cultured and further enriched on a large-scale; because the lytic bacteriophage specifically kills the drug-resistant vibrio harveyi, the problems of the antibiotics resistance due to abuse of antibiotics, antibiotics residue in animal bodies, and the like are prevented; the preparation of the present invention can be prepared into a spray solution or an eluting solution, and provides purification and protection effects for the sick stichopus japonicus, and the aquaculture water environment; the preparation of the present invention does not have any toxic and side-effects, such that the food-derived pollution is reduced, the security effect is provided for the food safety.

The invention discloses a bactericide for preventing and curing crop bacterial diseasess as well as a preparation method and application thereof. In the preparation method, oligosaccharide is added into bdellovibrio bacteriovorus solution to be evenly mixed to obtain the bactericide for preventing and curing crop bacterial diseasess. The invention applies bdellovibrio bacteriovorus solution for preventing and curing crop bacterial diseases for the first time, fills the vacancy of researching and developing bdellovibrio bacteriovorus at home and abroad and adds new species for biopesticide. Asbdellovibrio bacteriovorus has the characteristics of broad cracking pattern and quick growth and has the outstanding capability of eliminating various pathogenicbacteria and bacteria, after combinedwith oligosaccharide, the bdellovibrio bacteriovorus strengthens crop resistance, greatly improves pesticide effect and enlarges the preventing and curing spectrum of bacterial diseases. The bactericide prepared by the invention has no toxicity or pollution, is safe to natural enemies of crop diseases and pests, pathogenic bacteria have small possibility of producing resistance, so that the invention overcomes 3R problems brought by abusing antibiotics and avoids the defects of serious chemical pesticide pollution and ecological balance damage at present.

Antibiotics (Abx) are the world's most misused drugs. Antibiotics misuse occurs when the drug is administered in case of a non-bacterial infection (such as a viral infection) for which it is ineffective. Overall, it is estimated that 40-70% of the worldwide Abx courses are mis-prescribed. The financial and health consequences of Abx over-prescription include the direct cost of the drugs, as well as the indirect costs of their side effects, which are estimated at >$15 billion annually. Furthermore, over-prescription directly causes the emergence of Abx-resistant strains of bacteria, which are recognized as one of the major threats to public health today. This generates an immediate need for reliable diagnostics to assist physicians in correct Abx prescription, especially at the point-of-care (POC) where most Abx are prescribed. Accordingly, some aspects of the present invention provide methods using biomarkers for rapidly detecting the source of infection and administrating the appropriate treatment.

The invention discloses biological fermentation wheat bran with an antibacterial effect and a preparation method and application of the biological fermentation wheat bran. The preparation method of the biological fermentation wheat bran with the antibacterial effect comprises the following steps: (1) inoculating Paenibacillus sp. into a wheat bran culture medium and performing aerobic culture to obtain a fermented matter; and (2) drying the fermented matter obtained in the step (1) for preparation. The preparation method disclosed by the invention adopts a solid-state fermentation method, has the advantages of single strain and raw material, simple process and low cost, and is conductive to standardization of fermentation wheat bran quality and cost control of industrial large-scale production. The biological fermentation wheat bran with the antibacterial effect, prepared by the preparation method disclosed by the invention, can effectively relieve and even fundamentally change the present situation of abuse of antibiotics in existing animal cultivation industry after being used in animal feed industry.

The invention relates to synbiotics for feeds, a feed additive containing the same, and a feed and application thereof, belonging to the technical field of feed processing. The synbiotics for feeds use Lactobacillus plantarum, Enterococcus faecalis, Enterococcus faecium and Bacillus subtilis as composite probiotics and oligosaccharide as prebiotics; through synergism of the probiotics and the prebiotics, the prebiotics exert promotion effect on the probiotics, promote the growth of the probiotics and inhibit the growth of harmful bacteria, so the purpose of adjusting the microecological balance of intestinal tracts is eventually achieved; and residues are not left in animal bodies, and drug resistance is not incurred, so the problems of drug resistance and drug residues resulting from abuse of antibiotics are overcome. The feed provided by the invention has good effect on growing pigs and weaned piglings, accelerates the growth speed of bred pigs, improves the immunity of pigs and reduces the diarrhea rate and incidence rate of pigs.

The invention discloses a photosensitizer formula for photodynamically treating periodontitis and an application thereof. A hydrogel preparation for treating periodontitis takes toluidine blue as a photoactive substance and carbopol as a gel. A formula for optimizing the gel preparation comprises the following components by mass concentration: 0.05%-3% of carbopol, 0.001%-1% of toluidine blue, 0.01%-0.4% of sodium hydroxide or 0.07%-2% of triethanolamine and the balance of water. A method for photodynamically treating periodontitis, provided by the invention, can be used for replacing an antibiotic therapy, so that the problem of antibiotics abuse can be prevented; when the preparation is used for treating the oral diseases caused by infections, such as, periodontitis, the antibacterial rate of once irradiation treatment can reach up to 99.99%; serious patients can be repeatedly treated at intervals; and the photosensitizer formula is free from toxic or side effect to normal organ tissues and has wide market prospects and high application values.

The invention belongs to the technical field of aquaculture, and particularly relates to a special-purpose immunopotentiating agent for Cynoglossus semilaevis Gunther. The special-purpose immunopotentiating agent for the Cynoglossus semilaevis Gunther is formed by mixing the following main components in parts by weight: 0.2-0.4 part of beta-glucan, 4-12 parts of acanthopanax roots, 5-10 parts of ampelopsis grossedentata, 10-15 parts of lepidium meyenii, 12-15 parts of kluyveromyces fragilis, 10-20 parts of eucommia ulmoides leaves, 5-10 parts of malt, and 65-80 parts of bamboo vinegar. The abuse of antibiotics cannot be ignored in the industry of the aquaculture. The special-purpose immunopotentiating agent for the Cynoglossus semilaevis Gunther, disclosed by the invention, uses purely natural plant ingredients, and a small amount of beta-glucan is added, so that the immunopotentiation effect of a composition can be effectively enhanced; and in addition, the components do not contain the antibiotics, so that a series of consequences caused by the abuse of the antibiotics are avoided.

The invention discloses a DSF quorum sensing signal degrading bacteria, and applications thereof in plant disease control, and more specifically provides Burkholderia anthina bacterial strain HN-8 preserved at the Guangdong Culture Collection Center, on 24th, November, 2017, and the preservation number is GDMCCNO.60289. It is found in study that Burkholderia anthina, especially the bacterial strain HN-8 possesses remarkable and rapid degrading effect on quorum sensing signal molecule DSF, the degradation performance is stable, quorum sensing communication can be destroyed via degradation of DSF, and great application potential is achieved in prevention and treatment of black rotten caused by xanthomonas campestris, and pathogenic bacteria based on DSF. The Burkholderia anthina bacterial strain HN-8 also possesses relatively high drug resistance on ampicillin, carbenicillin, and streptomycin, so that pesticide or antibiotic abuse and drug resistance are avoided, and novel methods are provided for prevention of plant diseases.

The invention belongs to the technical field of feeds, and discloses a livestock and poultry fresh feed produced from a variety of dregs and a variety of bacteria by secondary fermentation and a preparation process thereof. The main technical characteristic of the invention is that the livestock and poultry fresh feed consists of 10 to 50 parts by weight of water, 4 to 6 parts by weight of soybean sauce dregs, 10 to 30 parts by weight of distilled spirit dregs, 5 to 15 parts by weight of beer dregs, 4 to 6 parts by weight of wine dregs, 5 to 15 parts by weight of wheat bran, 10 to 30 parts by weight of cottonseed meal, 5 to 15 parts by weight of soybean meal, 5 to 15 parts by weight of rapeseed meal, 5 to 15 parts by weight of peanut meal, 0.2 to 0.5 parts by weight of a variety of bacteria including bacillus subtilis, lactobacillus, saccharomycetes, aspergillus niger and chitosan oligosaccharide and 0.02 to 0.05 parts by weight of enzymic preparations. The livestock and poultry fresh feed solves the problems existing in the livestock and poultry breeding industry that food shortage caused by the contention between animals and human being for food and antibiotic misuse seriously threatening the health and life style of human being, and also solves the problems on how to open outlets for agricultural and sideline products and scientifically breed livestock and poultry, the livestock and poultry fresh feed is convenient to store for a long time, the feeding effect is good, and the cost is low.

The invention discloses a mold inhibitor for a fermentation bed and a preparation method for the mold inhibitor. The mold inhibitor consists of a composite chemical preparation and a composite microbial inoculum, wherein the composite chemical preparation is prepared by mixing an organic matter and an inorganic matter, and the composite microbial inoculum is prepared by performing single-strain pure fermentation on microbes and mixing. The mold inhibitor has the advantages that by the mold inhibitor, the removal rate of molds in the fermentation bed is over 90 percent, the adsorption rate of mycotoxins is 70 percent, the harm of the molds and the mycotoxins to poultry are reduced, the problem that livestock and poultry are poisoned by the molds or the mycotoxins due to environmental factors or feed factors in the feeding process is solved, and an effect of medicinal treatment is achieved, so that the problem that intestinal florae of the livestock and the poultry are unbalanced and the problem that antibiotics are abused in the conventional breeding process of the livestock and the poultry are solved.

The invention provides a traditional Chinese medicine composition for young poultry, a liquid-state fermented traditional Chinese medicine preparation and a preparation method thereof. The compositionis prepared from 30-38 parts of Astragalus membranaceus, 22-28 parts of rhizoma atractylodis macrocephalae, 20-25 parts of poria cocos, 22-28 parts of hawthorn and 18-25 parts of dandelions. The preparation comprises a composition and probiotics, wherein the probiotics are compound bacteria of clostridium butyricum, bacillus licheniformis and enterococcus faecalis. The preparation method comprises the step that the traditional Chinese medicine composition is prepared through probiotic fermentation. The traditional Chinese medicine preparation not only can increase the number of lactobacillusand bifidobacterium in intestinal tracts of young poultry to reduce the number of escherichia coli in the intestinal tracts and reduce the occurrence rate of diarrhea of the young poultry, but also can avoid the problem of high death and culling rate of the young poultry caused by abuse of antibiotics.

The invention discloses a hospital pathogen drug resistance monitoring system and method, the system comprises a collection module, a calculation module and an evaluation module; the collection module collects related data information, and transmits the collected related information to the calculation module; the calculation module performs data cleaning and standardization processing on the related collected information, obtains a drug resistance index by using a related algorithm, and transmits the drug resistance index to the evaluation module; the evaluation module performs evaluation according to the magnitude of the drug resistance index, obtains a related disease control risk analysis report, collects related data information, arranges the information and calculates the information to obtain the drug resistance index, and comprehensively judges the drug resistance condition of bacteria and the applicability of antibacterial drugs by using the drug resistance index. Therefore, the antibiotic abuse condition and the risk probability of generating super bacteria are effectively reduced.

The invention belongs to the technical field of microbial preparations, and particularly relates to a composite microbial preparation containing bacillus subtilis. The composite microbial preparationcontaining the bacillus subtilis comprises the following components in parts by weight: 40-60 parts of composite microbial powder, 20-30 parts of a composite carrier and 2-10 parts of a protective agent. According to the composite microbial preparation containing the bacillus subtilis, through the mutual coordination effect of bacillus subtilis, bacillus cereus and inulin bacillus, the side effects caused by antibiotic abuse are overcome, and a safe and green composite microbial preparation is provided for human beings; and meanwhile, the number of spores generated in the biological bacteriumfermentation process is effectively increased, and an important guarantee is provided for effectively prolonging the service life of the microbial agent and maintaining the pesticide effect and stability of the microbial agent in the subsequent preparation process of the biological bacterium preparation.

The invention discloses a method for detecting a gene cassette array in a bacterium integron by using an EcoRII enzyme digestion map library, which comprises the following steps of: firstly, carrying out enzyme digestion on the gene cassette array by adopting the restriction enzyme EcoRII when a new screened gene cassette array is determined; comparing the gene cassette array with the constructed EcoRII enzyme digestion map library so as to determine the novelty of the gene cassette array; and selecting connection, transformation and sequencing analysis so as to realize purposeful sequencing if maps in the library are not matched with the gene cassette array. The method is accurate, rapid and economic and is convenient for information sharing. The method is more suitable for relevant departments such as hospitals, quarantine inspection, forecast, preventive treatment and the like to realize the effective monitoring and prevent the outbreak prevalent of drug-resistant bacteria caused by the horizontal transferring and spreading of the drug-resistant bacteria containing the integron under the condition that antibiotic misuse is not well restrained.

The invention belongs to the technical field of poultry breeding and particularly relates to an immunopotentiator for commodity laying hens. The immunopotentiator for commodity laying hens is characterized by being prepared by mixing the following components in parts by weight: 0.1-0.3 part of pepper extract, 5-8 parts of manyprickle acanthopanax roots, 12-13 parts of ampelopsis grossedentata, 3-5 parts of yeast powder, 10-12 parts of astragalus membranaceus, 15-18 parts of malts and 35-40 parts of bamboo vinegar. In the existing breeding industry, antibiotics abuse cannot be ignored, and the immunopotentiator for commodity laying hens disclosed by the invention adopt pure natural plant components, and is supplemented with a little pepper extract, so that immunological enhancement effect of the composition can be effectively enhanced; the components do not contain antibiotics, so that a series of consequences caused by the antibiotics abuse are avoided. According to a test, the inventor finds that immunologic function of the poultry can be remarkably enhanced by adding the pepper extract, so that the poultry are not easily infected with a disease in the breeding process.

The invention provides a hedgehog-shaped catalytic material with an atomic catalytic center and application of the hedgehog-shaped catalytic material in preparation of antibacterial drugs, and belongs to the field of antibacterial drugs. The hedgehog-shaped catalytic material with the atomic catalytic center is obtained by mixing a hedgehog-shaped metal organic framework material with metal salt and a material with pyridine N and then carrying out heat treatment. The material can be used for killing drug-resistant bacteria MRSA by simulating a 'capturing and killing' process of macrophages, and also has good mimic oxidase activity, mimic peroxidase activity and mimic halogen peroxidase activity. The material not only has good antibacterial activity on drug-resistant bacteria MRSA in vitro, but also can quickly promote healing of animal skin wounds infected by the MRSA bacteria in vivo. The hedgehog-shaped catalytic material provided by the invention is a non-antibiotic material, has excellent biocompatibility, can solve the problem of bacterial drug resistance caused by antibiotic abuse, and has wide application prospects in preparation of biomimetic materials simulating macrophages and antibacterial drugs.

The invention relates to the technical field of livestock and poultry feed processing, in particular to antibiotic-free feed for sows and a preparation method of the feed. The antibiotic-free feed forsows comprises main materials, auxiliary materials, fermented auxiliary materials and supplements. The preparation method comprises the steps as follows: (1) fermenting the main materials; (2) preparing a traditional Chinese medicine liquid; (3) performing mixing to obtain an auxiliary material liquid; and (4) performing drying and granulation after uniform mixing. Bacillus and saccharomycetes microbial agents are used for fermentation, probiotics are used for improving immune capacity of pigs, and the risk of abuse of antibiotics is avoided. Meanwhile, a plant extract and a traditional Chinese medicine are added to the raw materials, the intestinal functions of sow herds can be improved, the digestive absorption rate can be increased, sows can be effectively promoted to lactation, and feeding of suckling piglets is promoted.

The invention discloses a fecal calprotectin or lactoferrin combined detection reagent and a preparation method thereof. The reagent comprises a PVC bottom plate, wherein a sample pad, a colloidal gold labeled pad, a nitrocellulose membrane detection pad and a water absorption pad are sequentially arranged at the top of the PVC bottom plate from left to right; a lactoferrin detection line, a calprotectin detection line and a quality control line are sequentially arranged on the internal surface of the nitrocellulose membrane detection pad from left to right; and the left side of the colloidal gold labeled pad is located on the inner side of the sample pad. The reagent can completely reflect intestinal inflammation, is used for auxiliary identification and diagnosis for distinguishing an inflammatory bowel disease and an irritable bowel syndrome, is used for early-stage screening of organic lesions such as intestinal tumors and polyps, is used for identifying acute bacterial diarrhea and nonbacterial diarrhea, can guide pharmacy, can avoid antibiotic abuse, is simple in detection technology, good in repeatability, stable in finished product structure, excellent in service performance and high in clinic practicability, and can partially replace the current invasive detection means.

The invention relates to the technical field of a biological preparation, and discloses lactobacillus gasseri for resisting enterohemorrhagic escherichia coli, an application of the lactobacillus gasseri, a composite probiotic preparation for poultry, prepared from the lactobacillus gasseri and an application of the composite probiotic preparation. For the lactobacillus gasseri (Lactobacillus gasseri) YD2-2 provided by the invention, the preservation No. is CCTCC NO:M 2019340, the lactobacillus gasseri particularly has efficient bacterial inhibition for the enterohemorrhagic escherichia coli,after the lactobacillus gasseri is mixed and blended with other bacterial strains, the composite probiotic preparation for poultry for resisting enterohemorrhagic escherichia coli can be prepared. Thepreparation can promote the growth of animals, the microbiological environment of intestines of the animals can be improved, the development of intestine mucosa can be promoted, the solubility IgA content and the serum IgG content of the intestine mucosa can be increased, the long-term sub-therapeutic dose and ultrahigh dose of antibiotics for preventing and treating animal diseases in feeds canbe reduced, and the damage in respects of bacterial drug resistance, drug residues of antibiotics, food safety and the like caused by indiscriminate use of the antibiotics can be avoided.

The invention discloses a Yanlixiao capsule and a preparation method thereof. The Yanlixiao capsule is prepared from clove leaves through processing and is a pharmaceutical composition. The pharmaceutical composition does not contain any other raw and accessory materials except for components from the clove leaves. The preparation method for the pharmaceutical composition is characterized by comprising the following steps: (1) extracting clove leaves with water and concentrating the obtained water extract into clear paste with relative density of 1.23 to 1.27 (80 DEG C); (2) crushing clove leaves and then carrying out screening with a sieve No. 5 so as to obtain fine powder; and (3) uniformly mixing the fine powder prepared in the step (2) with the clear paste prepared in the step (1) anddrying the mixture so as to obtain the pharmaceutical composition. The Yanlixiao capsule provided by the invention is a purely traditional Chinese medicine, has substantial curative effect and small side-effect, and can overcome the problem of abuse of antibiotics; and the Yanlixiao capsule is free of any accessory material and has substantial better dissolution and stability than commercially available Yanlixiao capsules.

The invention relates to the agriculture technical field, and relates to a land resource cycle renewable utilization agriculture production system; the system uses a biological method of farming livestock on a plowland in a fallow period to solve the plant production pesticide fertilizer abuse problems caused by existing land resource shortage conditions in our nation; the method uses cockroach nutritive values and medicinal values, adds cockroaches into animal fodder so as to replace antibiotic, thus solving antibiotic abuse problem in the existing breeding industry; two parts are combined to form the agriculture production system without pesticide, fertilizer, antibiotic and pollution, thus providing health effect.

The invention discloses an antibacterial peptide PR39 and PG1 co-expression vector and a method for preparing PR39 and PG1 transgenic mice. The antibacterial peptide PR39 and PG1 co-expression vector comprises a PR39 and PG1 co-expression control system and a screening gene EGFP expression control system. The method comprises the following steps: performing micro-injection on the PR39 and PG1 co-expression vector in mouse fertilized eggs, thereby obtaining the PR39 and PG1 transgenic mice. The transgenic mice are capable of realizing co-expression of the PR39 and PG1 and can achieve the effects of resisting attack of most of bacteria such as Gram negative bacteria and the like as well fungi, reducing usage of antibiotics and laying a foundation for obtaining transgenic livestock with PR39 and PG1 co-expression genes. If the livestock is capable of simultaneously expressing the antibacterial peptides PR39 and PG1, the usage of the antibiotics can be greatly reduced, and possibility is provided for solving antibiotics abuse and food safety problems.

The invention belongs to the technical field of microecological preparations, and particularly relates to a compound microecological preparation containing clostridium butyricum and a preparation method of the compound microecological preparation. The compound microecological preparation containing the clostridium butyricum comprises the following components in parts by weight: 40 to 65 parts of compound microorganism bacterium powder, 25 to 30 parts of a compound carrier, 3 to 6 parts of antibacterial peptide, 5 to 16 parts of a digestion promoter and 2 to 12 parts of an antioxidant. According to the compound microecological preparation containing the clostridium butyricum, the clostridium butyricum, lactobacillus acidophilus and bacillus licheniformis are compounded according to a specific proportion and are cooperated with the digestion promoter, so that side effects caused by antibiotic abuse are overcome, and a safe and green microorganism compound preparation is provided for human beings; and meanwhile, the oxidation resistance of the compound microecological preparation is improved, the service life of the compound microecological preparation is prolonged, and the long-termstorage activity of the compound microecological preparation is improved.

The present invention discloses one kind of beta-lactamase SHV genovariation detecting chip. The beta-lactamase SHV genovariation detecting chip includes a solid phase carrier and a probe, which is made to hybridize with the nucleotide sequence of the beta-lactamase SHV gene to be detected and / or its complementary sequence. The present invention also discloses the method of using the chip in detecting beta-lactamase SHV genovariation. The chip of the present invention may be used in simultaneous detection of several kinds of beta-lactamase SHV genovariation and in detection of bacterial drug resistance and resisted drug kinds for directing reasonable drug use and avoiding abuse of antibiotics.

The invention relates to a virus delivery culture medium. The virus delivery culture medium contains the following components in concentration: Hank'S equilibrium liquid, 0.2-2 g / L of amino acid, 5-50% of glycerol, 0.02-0.2% of a ProClin preservative and 2-15 g / L of bovine serum albumin. The virus delivery culture medium has the beneficial effects that the virus delivery culture medium is based on the Hank's equilibrium liquid, amino acid is added as a virus protein shell protection component, glycerol is added, the protection of the complete form of a virus in a long-term low-temperature stored virus sample is facilitated, the antigen activity of a virus shell is facilitated, and the virus delivery culture medium is suitable for antigen detection and nucleic acid detection of the virus; and meanwhile, the preservative is added, so that the virus delivery culture medium is suitable for virus transportation and long-term preservation. The virus delivery culture medium has no interference on the detection of an in-vitro diagnostic reagent, avoids the abuse of antibiotics, and reduces the drug resistance risk of pathogenic bacteria.