43 results about "Antibiotic misuse" patented technology

The invention discloses a novel acinetobacter baumannii phage vBAbaSqsb1 and application thereof, and belongs to the technical field of microorganisms. The bacteriophage is an Acinetobacter baumannii bacteriophage vBAbaSqsb1 which is preserved in the Guangdong Microbiological Culture Collection Center on July 5, 2024, the preservation number is GDMCC (China General Microbiological Culture Collection Center) NO: 64831-B1, and the preservation address is Institute of Microbiology of Academy of Sciences of Guangdong Province. The bacteriophage can specifically recognize and split the acinetobacter baumannii so as to eliminate infection and overcome the problem of antibiotic resistance of the acinetobacter baumannii. Through discovery and application research of the phage vBAbaSqsb1, the phage vBAbaSqsb1 not only can be used for preparing a phage preparation for preventing and treating diseases caused by acinetobacter baumannii infection, but also can be used for preparing various environmental bactericides for solving potential acinetobacter baumannii in various environments. The bacteriophage vBAbaSqsb1 has the characteristics of safety, no toxic or side effect and the like, so that the problem of drug resistance caused by abuse of antibiotics can be avoided when the bacteriophage vBAbaSqsb1 is applied.

The invention discloses a Min pig source lactobacillus reuteri LRM2293 strain and an application of the Min pig source lactobacillus reuteri LRM2293 strain in anti-inflammatory reaction. The Min pig source lactobacillus reuteri is named as Lactobacillus reuteri LRM2293, and is preserved in the China Center for Type Culture Collection, the address is Wuhan University, Wuhan, China, the preservation number is CCTCC NO: M 20242331, and the preservation time is October 25, 2024. Research finds that the lactobacillus reuteri LRM2293 has the capacity of transforming bile acid in vitro and can be used as a candidate strain of probiotics. In vitro, a culture supernatant of the lactobacillus reuteri LRM2293 can be used for reducing the inflammatory reaction of IPEC-J2 cells; in vivo, the LRM2293 strain can be colonized in each intestinal segment of a piglet, and can repair an intestinal barrier and relieve intestinal injury of the inflammatory piglet, so that the problem of antibacterial drug resistance caused by abuse of antibiotics is expected to be reduced, and a technical means is provided for development of a novel functional product.

The invention relates to an antibiotic-free feed additive for breeding crucian carps and a preparation method of the antibiotic-free feed additive, and belongs to the technical field of feed additives. According to the feed additive, seaweeds and Chinese herbal medicines are used as raw materials and are subjected to staged fermentation with compound probiotics, a phagostimulant is added, and freeze drying is performed to prepare the antibiotic-free crucian breeding feed additive, so that the prevalence rate of crucian can be reduced, the survival rate can be increased, and the growth of crucian can be accelerated; the problem of drug resistance caused by antibiotic abuse in crucian breeding in the prior art can be solved, and unification of high antibacterial activity, biological safety and environmental friendliness in crucian breeding is achieved.

The invention relates to the technical field of blood culture bottles, in particular to a composition and application thereof in preparation of a microbial culture medium. Yeast extract powder, soy peptone and casein tryptone are combined into a nitrogen source, and an aerobic and anaerobic culture medium for clinical pathogenic microorganism culture is formed. Through comparison verification, in growth experiments of nine aerobic bacteria and seven anaerobic bacteria, the culture speed and the biomass accumulation of the aerobic and anaerobic culture media added with the composition are integrally superior to those of other components excluded in the prior art and screening. Therefore, the composition can effectively promote the performance of a blood culture product, improve the clinical blood flow infection detection capability, inhibit antibiotic abuse and save the life of a patient.

The invention relates to the field of medical detection, in particular to a fluorescent sensing composition, a fluorescent sensing film and application thereof. According to the invention, by screening a plurality of fluorescent indicators and combinations thereof, an indicator formula with simple components and low cost is finally obtained. Based on the formula, by optimizing the preparation process, the high-efficiency fluorescent sensing film resistant to damp-heat sterilization is developed. After a microorganism culture bottle prepared on the basis of the membrane is inoculated with Gram-negative bacilli, positive cocci and yeast fungi representative quality control strains, experiments of full-automatic microorganism culture system fluorescence curve determination, Gram staining, microorganism mass spectrum identification and the like prove that the fluorescence sensing membrane is excellent in detection performance. The fluorescent sensing film provided by the research is applied to clinical microorganism detection, can provide technical support for blood flow infection diagnosis, and is of great significance in suppressing antibiotic abuse and improving prognosis of blood flow infection patients.

The invention provides a vibrio parahaemolyticus bacteriophage RKP-VP23248 and application of the vibrio parahaemolyticus bacteriophage RKP-VP23248, the vibrio parahaemolyticus bacteriophage is preserved in the China General Microbiological Culture Collection Center on September 27, 2024, and the preservation number of the vibrio parahaemolyticus bacteriophage RKP-VP23248 is CGMCC (China General Microbiological Culture Collection Center) No. 46193. The invention further provides a bacteriophage composition, and the bacteriophage composition comprises the vibrio parahaemolyticus bacteriophage RKP-VP23248. The bacteriophage provided by the invention is separated from natural water environments such as seawater, river water and water sediment, still keeps high-level titer under continuous passage, has relatively strong genetic stability and thermal stability, and can effectively cope with disease outbreak caused by mass propagation of pathogenic bacteria when water environment conditions change. The bacteriophage has strong lysis property on the drug-resistant high-pathogenicity vibrio, the lysis rate is up to 88.51%, and the problem of vibrio drug resistance enhancement caused by antibiotic abuse can be effectively solved, so that the problems of large-scale acute death and the like of aquatic animals such as fishes, shrimps and the like caused by the high-pathogenicity vibrio are solved.

The invention discloses pediococcus pentosaceus TRM54998 with broad-spectrum antibacterial activity and application of the pediococcus pentosaceus TRM54998, and belongs to the field of microorganisms. The strain is preserved in the China Center for Type Culture Collection (CCTCC) on June 04, 2024, and the preservation number is CCTCC NO: M 20241142. It is proved that the strain has good acid resistance and bile salt resistance and can grow in the environment with the bovine bile salt concentration being 0.1-0.2% (w / v) or in the pH value range being 3-7; the fermentation supernatant has an inhibition effect on nine pathogenic bacteria such as salmonella and the like, and the antibacterial effect is stable; meanwhile, experiments prove that the pediococcus pentosaceus TRM54998 has no wide drug resistance, can relieve the problems of aggravated drug resistance, damage to microbial ecological balance and the like caused by antibiotic abuse, provides a strain resource for preparing a biological bacteriostatic agent or an intestinal probiotic agent, and has a wide application prospect.

The invention discloses application of triptolide ketone in preparation of a medicine for treating sepsis lung injury, and belongs to the technical field of biological medicine. Aiming at the problems that traditional chemical synthetic drugs may cause a large amount of sequelae and antibiotic abuse, and triptolide may cause liver and kidney injury when treating sepsis, research finds that triptolide can be used for treating sepsis lung injury, and can be used for treating sepsis lung injury. According to the application, the protein expression level of HIF-1alpha in proinflammatory activated macrophages is lowered, so that aerobic glycolysis is inhibited, and the effect of treating sepsis lung injury is achieved. Compared with the triptolide alcohol, the triptolide ketone does not cause liver and kidney injury while playing a role in treating the sepsis lung injury, and the curative effect of the triptolide ketone is equivalent to that of the existing medicine 2ME2 for treating the sepsis lung injury, so that the triptolide ketone has important application potential clinically, and can be used for preparing medicines for treating the sepsis lung injury. And a theoretical basis is provided for clinically developing medicines for treating sepsis lung injury.

The invention relates to the technical field of microorganisms, in particular to a probiotic product for inhibiting salmonella in a chicken body and a screening method, the probiotic product comprises Chencity bacillus sachariensis, and the colony count of the Chencity bacillus sachariensis in the probiotic product is 107-1010 CFU / mL. Based on the screening method of the probiotics for inhibiting the salmonella in the chicken body, the screened city bacillus sachariensis is prepared into the probiotic product for inhibiting the salmonella in the chicken body, so that the salmonella infection condition of the chicken can be effectively improved, the weight of the chicken with positive salmonella infection is increased, and the salmonella in the chicken body is inhibited. The salmonella loading quantity in chicken bodies is obviously reduced, the salmonella loading quantity has the characteristics of low residual quantity and environment friendliness, and the problems of antibiotic residues, bacterial drug resistance increase and the like caused by antibiotic abuse can be avoided. By adopting the screening method of the probiotics for inhibiting the salmonella in the chicken body, provided by the invention, the dominant probiotics capable of inhibiting the salmonella can be simply, conveniently and efficiently determined.

A new antibacterial peptide of Japanese eel A1 Cath2b 143‑174 and its application, belong to the field of biotechnology. The antibacterial peptide is a Cathelicidin family antibacterial peptide of Japanese eel A1 The 143-174 amino acid fragment of Cathelicidin 2b is named as Cath2b A1 Cath2b 143‑174 , and the amino acid sequence is shown as SEQ ID NO. 3. The antibacterial peptide has high inhibition / killing activity on various aquatic pathogenic bacteria, has excellent thermal stability and salt ion tolerance, and has no obvious toxicity to host cells; it plays a bactericidal role by destroying the bacterial cell membrane, and can also attract host immune cells to enhance immunity. The antibacterial peptide can be applied in the preparation of aquatic animal bacterial disease prevention and control drugs. It can solve the problems of drug resistance and drug residue caused by the abuse of traditional antibiotics, and is convenient to prepare, has strong adaptability, and is suitable for the prevention and control of bacterial diseases of aquatic animals.

The invention discloses a strain of leuconostoc mesenteroides V7, and the microbial preservation number is CGMCC (China General Microbiological Culture Collection Center) No.34824. The leuconostoc mesenteroides V7 disclosed by the invention is applied to products for inhibiting source avian pathogenic escherichia coli. The leuconostoc mesenteroides V7 disclosed by the invention has an inhibition effect on avian pathogenic escherichia coli, so that the leuconostoc mesenteroides V7 can be used for replacing antibiotics in poultry production and breeding to treat avian pathogenic colibacillosis. The invention provides a novel antibiotic substitute, which can obviously improve the infection condition of avian pathogenic escherichia coli in poultry bodies and avoid the problems of antibiotic abuse and bacterial drug resistance as much as possible.

Marine pollution caused by antibiotic abuse has become a global environmental challenge, wherein trace detection of quinolone antibiotics (such as fleroxacin FLE, norfloxacin NOR and enrofloxacin ENR) is particularly key. The invention develops a high-stability fluorescence sensor based on a lanthanide series metal organic framework (Ln-MOF (at) BTEC), and by introducing a hydrogen bond network strengthening strategy, the performance of the material in a seawater high-salt environment is remarkably improved. The fluorescence decay rate of the material in simulated seawater within 60 min is as low as 2.67%, which is 21.49% in 30 min compared with that of a base material, and the stability is obviously improved. In addition, the ratio proportional fluorescence sensing can effectively and instantaneously respond to FLE, NOR and ENR in the presence of numerous interferents in a seawater system, and the detection limits within the range of 0-10 [mu] M are 80.7 nM, 21.03 nM and 28.22 nM respectively. Meanwhile, an intelligent mobile phone platform is successfully integrated to realize visual field detection, and the standard addition recovery rate in Dalian coastal areas and seawater in culture areas reaches 83-115% (RSDlt; the research provides a novel solution with high stability and high sensitivity for marine antibiotic pollution monitoring.

The invention discloses an application of lactobacillus plantarum RT1 with an antibacterial effect. The lactobacillus plantarum RT1 disclosed by the invention has excellent acid-resistant and cholate-resistant characteristics and broad-spectrum and efficient antibacterial action, can be used as a novel efficient antibacterial probiotic candidate, and is applied to the development of food preservatives, functional foods (such as fermented milk and probiotic solid beverages), feed additives and health-care products related to intestinal health; and a new microbial resource is provided for solving the problem of intestinal flora imbalance caused by food spoilage and antibiotic abuse.

The invention relates to the technical field of microorganisms, in particular to a method for reducing the dosage of antibiotics for treating clostridium perfringens infection, a bactericidal composition, bacteriophage and application. According to the method, the clostridium perfringens bacteriophage RDP-CP-2310008 and one or more antibiotics are used in a combined mode, and the clostridium perfringens bacteriophage RDP-CP-2310008 and the one or more antibiotics are used in a combined mode. The clostridium perfringens bacteriophage RDP-CP-2310008 is preserved in the China General Microbiological Culture Collection Center on November 29, 2023, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.45778. The clostridium perfringens bacteriophage RDP-CP-2310008 can be used The bactericidal composition is prepared from the following effective components: the clostridium perfringens bacteriophage RDP-CP-2310008, and one or more antibiotics used for resisting the clostridium perfringens. The invention further discloses application of the clostridium perfringens phage RDP-CP-2310008 in preparation of a medicine for preventing and / or treating clostridium perfringens infection. The invention provides and verifies that the specific bacteriophage is combined with the antibiotics for the first time, so that the dosage of the antibiotics can be greatly reduced and can be up to 64 times, and a brand-new path is provided for solving the problem of abuse of the antibiotics in the breeding industry.

The invention provides a Shewanella algal bacterium phage (Shewanella algal bacterium phage) PKP-SH23103 and application of the Shewanella algal bacterium phage. The Shewanella algal bacterium phage is preserved in the China General Microbiological Culture Collection Center on November 29, 2023, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.45783. The invention further provides an application of the Shewanella algal bacterium phage PKP-SH23103. The invention further provides a bacteriophage composition, and the bacteriophage composition comprises the algal shewanella bacteriophage PKP-SH23103. The phage provided by the invention is separated from a natural water environment, still keeps high-level titer (1010PFU / mL) under continuous passage, has relatively strong genetic stability and thermal stability, has the characteristic of high permeability resistance, and can effectively cope with disease outbreak caused by mass propagation of pathogenic bacteria when water environment conditions change; the bacteriophage obtained by the invention can be used for preventing and treating diseases caused by shewanella alginate, is safe and environment-friendly, effectively replaces the use of antibiotics in a culture process, reduces the problems of water body pollution, enhanced bacterial drug resistance, drug residues and the like caused by antibiotic abuse, and has good practical application value.

The application discloses an antagonistic bacterium of pathogenic shewanella of Apostichopus japonicus and application thereof, and belongs to the technical field of aquatic microorganisms. Gilvus japonicus The antagonistic bacterium is separated from the intestinal tract of healthy Apostichopus japonicus and is identified as Japanese Yellow Sea Oceanic Bacterium (Shewanella japonica), has no beta-hemolysis characteristic, is safe and non-toxic to Apostichopus japonicus, and can significantly antagonize the pathogenic bacterium Shewanella of Apostichopus japonicus skin rot syndrome. The application also discloses application of the antagonistic bacterium, which can be prepared into an aquatic microecological preparation for preventing and treating the skin rot syndrome of Apostichopus japonicus, and is applied through intraperitoneal injection or immersion, so that the survival rate of Apostichopus japonicus under exposure of the pathogenic bacterium is significantly improved. The strain has strong salt and alkali resistance, tolerances to various antibiotics and chemical drugs, and strong environmental adaptability, can be applied to the cultivation of Apostichopus japonicus to replace traditional antibiotics, effectively solves the problems of food safety and environmental pollution caused by the abuse of antibiotics, and has good industrial application prospect.

The invention discloses an application of Akkermansia muciniphila in the preparation of a product for resisting klebsiella pneumoniae infection, and particularly discloses an application of Akkermansia muciniphila in the preparation of a product for resisting klebsiella pneumoniae infection. The invention provides an application of A. muciniphila bacteria or a culture solution thereof or any one of the following applications: 1) preparing a product for resisting host infection with klebsiella pneumoniae; 2) preparing a product for reducing the pathogenicity of klebsiella pneumoniae to a host; (3) preparing a product for improving the Klebsiella pneumoniae infection resistance of a host; and 4) application in preparation of a product for activating the AhR signal channel of the host intestinal tract. Compared with the traditional antibiotic therapy, the A. muciniphila, as the emerging second-generation probiotics, can effectively resist klebsiella pneumoniae infection, can reduce the dosage of antibiotics, and provides a new method for improving sharp increase of drug-resistant bacteria and difficulty in treatment caused by antibiotic abuse.

The invention relates to an anti-biofilm peptide and application thereof, and belongs to the technical field of antibacterial peptides. The invention relates to an anti-biofilm peptide VKLR. The amino acid sequence of the anti-biofilm peptide VKLR is as shown in SEQ ID NO: 1: LVKLRVKLRVKLR. The broad-spectrum antibacterial agent aims at bacteria including gram-negative bacteria and gram-positive bacteria. The anti-biofilm peptide VKLR disclosed by the invention has broad-spectrum antibacterial activity and has a remarkable antibacterial effect on various gram-negative bacteria and gram-positive bacteria such as escherichia coli, pseudomonas aeruginosa, staphylococcus aureus and staphylococcus epidermidis, and infection caused by a pathogen biofilm seriously threatens human and animal health; in the breeding process, abuse of traditional antibiotics is likely to generate drug resistance risks, and people pay attention to novel antibacterial drug substitutes. According to the present invention, the 14-element library of the AMPs is obtained, the peptide VKLR shows the huge potential in the antibacterial effect aspect, and is the ideal candidate for treating bacterial infection, such that the research provides the new insight for the future design of the biofilm removing peptide, and the wide application of the biofilm removing peptide in the medicine and the animal husbandry is promoted.

The invention discloses application of thiamine in improving the sugar utilization rate and growth performance of sturgeons, and belongs to the field of aquaculture. It is proved for the first time that by enhancing a glycolytic pathway, inhibiting a gluconeogenesis pathway and promoting glycogen decomposition, the thiamine effectively improves the sugar metabolism capacity of sturgeons and reduces hepatic glycogen deposition and fat accumulation, so that the risk of metabolic disorder is reduced. Meanwhile, thiamine also promotes muscle fiber growth, so that the growth speed of the sturgeons and the feed utilization rate are remarkably increased. Therefore, the thiamine can be used as a feed additive for preparing the feed for improving the sugar utilization rate and the growth performance of the sturgeons. A safe and efficient nutrition regulation and control scheme is provided for sturgeon culture, the feed utilization rate and culture benefits are improved, meanwhile, ecological risks such as antibiotic abuse are avoided, and remarkable economic benefits and application prospects are achieved.

The invention belongs to the technical field of aquaculture science, microbiology, immunology and bioengineering, and particularly relates to application of endophytic monad in serving as disease-resistant probiotics of shellfish. The added endophytic monad has a protection effect on the pathogen infected shellfish, the survival rate of the shellfish can be remarkably increased by adopting a seawater direct adding method of the two endophytic monad, namely a pre-adding method and a continuous adding method, and although the two tested adding methods have remarkable protection effects, the survival rate of the shellfish can be remarkably increased. However, compared with a continuous adding method, the pre-adding method is simpler, more convenient and more labor-saving in operation, and the pre-adding method is more recommended to be adopted in practical application; the principle for realizing protection is related to the growth inhibition of the endophytic monad on pathogenic bacteria, the reduction of host shellfish tissue damage and the improvement of the phagocytic activity of host shellfish blood cells. The invention provides a new strategy for improving disease resistance of shellfish and reducing disease loss of shellfish aiming at the current situations of difficulty in disease prevention and control and antibiotic abuse in current aquaculture, and can be applied to scenes such as healthy culture of shellfish and the like.

The invention discloses a health-care pig feed containing Chinese herbal medicines and a preparation method thereof, the health-care pig feed comprises a basal feed and a Chinese herbal medicine composition, and the Chinese herbal medicine composition is prepared from the following raw materials in parts by weight: 10-20 parts of astragalus membranaceus, 5-10 parts of liquorice, 5-10 parts of honeysuckle, 10-20 parts of hawthorn, 10-15 parts of malt, 10-15 parts of medicated leaven, 5-10 parts of pericarpium citri reticulatae and 10-15 parts of poria cocos; the total weight of the Chinese herbal medicine composition accounts for 5-15% of the total weight of the health-care pig feed. The invention belongs to the technical field of feeds, and particularly provides a pig feed for solving the problems of low immunity caused by single nutrition of a traditional pig feed and drug residues and bacterial drug resistance caused by abuse of antibiotics.

The invention discloses application of parabacteroides dieldrii in preparation of a medicine for treating prostatic cancer, and belongs to the field of biological medicine. It is found for the first time that the abundance of parabacteroides dieldrii in intestinal flora of a prostate cancer patient is remarkably reduced, and the parabacteroides dieldrii is selected as potential probiotics for inhibiting growth of prostate cancer tumors. The result of a parabacteroides dieldrii single bacterium colonization experiment shows that the parabacteroides dieldrii and the metabolite gamma-linolenic acid (GLA) thereof can efficiently inhibit the growth of the prostatic cancer tumor, can also improve the condition that the treatment effect of docetaxel on the prostatic cancer is poor due to abuse of clinical antibiotics, and can be prepared into the medicine for treating or assisting in treating the prostatic cancer. The invention provides a new treatment strategy and medicine for the treatment of the prostatic cancer, and also provides a brand new medicine development path and a clinical application scheme for the micro-ecological treatment of the prostatic cancer.

The invention discloses application of combination of emodin and antibiotics in preparation of a medicine for preventing and treating salmonella infection, and belongs to the field of biological medicine. According to the application disclosed by the invention, on the premise of not influencing the proliferation and activity of intestinal epithelial cells, the emodin is respectively combined with ciprofloxacin and tetracycline with minimum inhibitory concentration to achieve a remarkable synergistic effect of inhibiting salmonella from invading the intestinal epithelial cells. The invention provides combination of emodin and ciprofloxacin or tetracycline, application of the emodin and ciprofloxacin or tetracycline in preparation of drugs for inhibiting salmonella from invading intestinal epithelial cells and application of the emodin and ciprofloxacin or tetracycline in preparation of drugs for preventing and treating salmonella infection. According to the invention, in the prevention and treatment of salmonella infection, the risk of antibiotic abuse is reduced by using emodin to reduce and replace antibodies.

The invention belongs to the technical field of prawn culture, and particularly relates to a method for treating vibrio diseases of litopenaeus vannamei seedlings through biological floccules. The method comprises the following steps: (1) preparing runway type biofloc; (2) preparing functional biofloc; and (3) treating vibrio diseases in opossum shrimp and larval shrimp stages. The vibrio disease at the big seedling stage is treated. The invention provides an effective method for treating vibrio diseases of prawn seedlings without using antibiotics, solves the problem of antibiotic abuse in the seedling raising process, and has important significance in promoting green and healthy development of the prawn seedling raising industry.

The application provides a high positive charge self-assembled ruthenium complex targeting electron transport chain and a preparation method and application thereof, and belongs to the technical field of biological medicines.The ruthenium complex provided by the application has a structural formula I shown in formula I; wherein 1<=n<=15.The high positive charge self-assembled ruthenium complex targeting electron transport chain is synthesized for the first time, the complex shows high-efficiency anti-MRSA activity, and can eliminate bacteria without toxic side effects, thereby showing good high-efficiency low-toxicity antibacterial activity, and providing a novel high-efficiency high-biocompatibility antibacterial strategy for solving problems such as antibiotic abuse and antibiotic resistance of bacteria.

The present invention relates to an antimicrobial adjuvant containing a biphenyl derivative compound as an active ingredient, and a technology of various uses thereof. The compound of the present invention reduces the dosage of polymyxin antibiotics, which are administered to inhibit the proliferation of gram-negative bacteria, by enhancing the sensitivity of the gram-negative bacteria with respect to the polymyxin antibiotics, is concomitantly administered with the polymyxin antibiotics to show gram-negative bacteria growth inhibitory and killing effects, may notably reduce side effects such as nephrotoxicity, and may prevent or treat sepsis and septic shock due to antibiotic overuse.

The invention belongs to the technical field of antibacterial drugs, and discloses a synergistic antibacterial composition based on a pepper extract and application thereof. The composition is composed of a Chinese prickly ash extract and cefoperazone or ampicillin, active ingredients of the Chinese prickly ash extract comprise various compounds such as ZP-amideC and the like, and the content of each ingredient is definitely required. The active ingredients of Chinese prickly ash are extracted through a heating reflux method, and tests prove that the composition has a remarkable synergistic inhibition effect on drug-resistant escherichia coli and can effectively inhibit bacterial proliferation and biofilm formation. A trace dilution chessboard experiment shows that the antibacterial effect of the combined drug is better than that of the single drug, for example, the combined use of 50 mu g / mL of pepper extract and 200 mu g / mL of cefoperazone can completely inhibit the proliferation of drug-resistant Escherichia coli to 24 hours. The invention provides a new thought for solving drug-resistant bacteria crisis caused by antibiotic abuse, has a good application prospect in preparation of antibacterial drugs, inhibition of drug-resistant escherichia coli biofilm formation and the like, and provides an effective way for prevention and control of pathogenic microorganisms and delay of drug resistance development.

The invention provides a molybdenum-based nano composite material as well as a preparation method and application thereof. The molybdenum-based nano composite material comprises molybdenum disulfide nanoflowers, molybdenum disulfide nanosheets, molybdenum disulfide nanodots, molybdenum oxide nanoparticles, carbon-based molybdenum single atoms and molybdenum-based polyoxometallate nanoparticles. The molybdenum-based nano composite material mediated and adjustable by the physiological microenvironment is designed, the material integrates the efficient antibacterial performance similar to that of traditional antibiotics, intestinal flora disorder caused by use of the antibiotics can be remarkably recovered, the relative abundance of probiotics is improved, the problem of intestinal flora imbalance caused by abuse of the antibiotics is solved, and the effect of improving the intestinal flora imbalance is achieved. The application effect of far-end tumor treatment can be achieved. The material is suitable for industrialization and has a huge market demand prospect.

The present invention provides a Shewanella algae bacteriophage PKP-SH23103 and its application. The Shewanella algae bacteriophage was deposited in the China General Microbiological Culture Collection Center on November 29, 2023, with a deposit number of CGMCC NO.45783. The present invention also provides a phage composition comprising the Shewanella algae bacteriophage PKP-SH23103. The phage described in the present invention is isolated from a natural water environment and maintains a high level of titer (10 10 PFU / mL), has strong genetic stability and thermal stability, and is resistant to hyperosmosis, and can effectively respond to disease outbreaks caused by the massive reproduction of pathogens when water environmental conditions change. The bacteriophage obtained by the present invention can be used for the prevention and treatment of diseases caused by Shewanella algae, is safe and environmentally friendly, and effectively replaces the use of antibiotics in aquaculture, reducing water pollution caused by the abuse of antibiotics, enhanced bacterial resistance, and drug residues, and has good practical application value.

The feed is prepared from more than 100 Chinese herbal medicines such as herba ephedrae, cassia twigs, radix astragali, honeysuckle flowers and the like, and the Chinese herbal medicines take effect synergistically according to a specific ratio. The preparation method comprises the following steps: cleaning the medicinal materials, cooking at 100 DEG C for 30 minutes, drying until the water content is 12-14%, crushing, stirring and mixing, granulating and sterilizing. The invention belongs to the technical field of feeds, and particularly provides a feed for solving the problems of low immunity caused by single nutrition of a traditional sheep feed, drug residue and bacterial drug resistance caused by abuse of antibiotics, insufficient efficacy of an existing Chinese herbal medicine feed and the like.