Mannose functionalized protein gold nano-cluster, and preparation method and application thereof

A mannose, protein gold technology, applied in chemical instruments and methods, nanotechnology for materials and surface science, nanotechnology, etc., can solve the problem of gold nanoclusters with toxicity, limited application and poor biocompatibility And other issues

Active Publication Date: 2020-04-24
EZHOU INST OF IND TECH HUAZHONG UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies of the above-mentioned prior art, the present invention provides a protein gold nanocluster functionalized with mannose and its preparation method and application, so as to solve the problem of using chemical reagents to synthesize gold nanoclusters in the prior art, so that the gold nanoclusters have Toxicity, poor biocompatibility, resulting in limited application

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  • Mannose functionalized protein gold nano-cluster, and preparation method and application thereof
  • Mannose functionalized protein gold nano-cluster, and preparation method and application thereof
  • Mannose functionalized protein gold nano-cluster, and preparation method and application thereof

Examples

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Embodiment 1

[0059] Example 1 provides a method for preparing protein gold nanoclusters functionalized with mannose, the method comprising: mixing 100 mg of bovine serum albumin, 30 mg of NH 2 - Mannose and 10 mg of EDC were sequentially added to 5 ml of phosphate buffer solution with a pH value of 7, stirred at 25 degrees Celsius for 12 hours, purified with an ultrafiltration tube with a molecular weight cut-off of 10 kDa, and then concentrated to obtain mannose- Bovine Serum Albumin Conjugate. The mannose-bovine serum albumin conjugate was redissolved in 2 ml of phosphate buffer, and 3 ml of chloroauric acid solution with a concentration of 8 mmol was added. After stirring for 2 minutes, 200 microliters of a concentration of 1 mol / L of sodium hydroxide solution, adjust the pH value to 11, stir at 37 degrees Celsius for 12 hours, purify and concentrate with an ultrafiltration tube with a molecular weight cut-off of 10kDa to obtain protein gold nanoclusters, and disperse the protein gold ...

Embodiment 2

[0062] Example 2 provides a method for preparing mannose-functionalized protein gold nanoclusters, the method comprising: 90 mg of bovine serum albumin, 30 mg of NH 2 -Mannose and 10 mg of EDC were sequentially added to 5 ml of 4-hydroxyethylpiperazineethanesulfonic acid buffer with a pH value of 7, stirred at 30 °C for 14 hours, and then filtered with an ultrafiltration tube with a molecular weight cut-off of 10 kDa Concentrate after purification to obtain mannose-bovine serum albumin conjugate. Re-dissolve the mannose-bovine serum albumin conjugate in 3 ml of 4-hydroxyethylpiperazineethanesulfonic acid buffer, add 3 ml of chloroauric acid solution with a concentration of 9 mmol, stir for 2 minutes, and then Add 200 microliters of sodium hydroxide solution with a concentration of 1mol / L, adjust the pH value to 10, stir at 38 degrees Celsius for 15 hours, purify with an ultrafiltration tube with a molecular weight cut-off of 10kDa, and concentrate to obtain protein gold nanocl...

Embodiment 3

[0065] Example 3 provides a method for preparing mannose-functionalized protein gold nanoclusters, the method comprising: mixing 110 mg of bovine serum albumin, 30 mg of NH 2 - Mannose and 10 mg of EDC were sequentially added to 5 ml of phosphate buffer with a pH value of 7, stirred at 35 degrees Celsius for 11 hours, purified with an ultrafiltration tube with a molecular weight cut-off of 10 kDa, and then concentrated to obtain mannose- Bovine Serum Albumin Conjugate. Redissolve the mannose-bovine serum albumin conjugate in 2 ml of phosphate buffer, add 3 ml of a 10 mmol / m chloroauric acid solution, stir for 2 minutes, then add 200 μl of a 1 mol / L of sodium hydroxide solution, adjust the pH value to 10, stir at 30 degrees Celsius for 14 hours, purify and concentrate with an ultrafiltration tube with a molecular weight cut-off of 10kDa to obtain protein gold nanoclusters, and disperse the protein gold nanoclusters in 5 ml of phosphate buffer.

[0066] The excitation wavelen...

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Abstract

The invention discloses a mannose functionalized protein gold nano-cluster, and a preparation method and an application thereof. The method comprises the following steps: S1, bovine serum albumin andNH2-mannose are dissolved in a buffer solution and then are mixed with EDC, and stirring, purification and concentration are carried out in sequence to obtain a mannose-bovine serum albumin conjugate,wherein the mass ratio of the bovine serum albumin to the NH2-mannose to the EDC is (9-11):3:1; and S2, the mannose-bovine serum albumin conjugate is mixed with a chloroauric acid solution in a buffer solution, the pH value is adjusted, and stirring and purifying are sequentially carried out to obtain the protein gold nano-cluster. The preparation method of the protein gold nano-cluster is convenient, simple, rapid and efficient, and the protein gold nano-cluster synthesized by the method is nontoxic, good in biocompatibility, good in dispersity, strong in fluorescence and high in detection sensitivity.

Description

technical field [0001] The invention belongs to the technical field of bionano materials, and in particular relates to a mannose-functionalized protein gold nanocluster and a preparation method and application thereof. Background technique [0002] Gold nanoclusters refer to the core composed of several to dozens of Au noble metal atoms, the size is close to the Fermi wavelength of electrons, and the surface is stabilized and stabilized by ligands such as thiol small molecules, nucleic acids, polymer macromolecules or biomacromolecules. Protected, a fluorescent nanoparticle that emits fluorescence when excited by energy. Gold nanoclusters have been extensively studied and applied due to their excellent fluorescence properties, non-toxicity, and high biocompatibility. [0003] The existing synthesis method of gold nanoclusters generally adopts the core-etching technique. First, a mild reducing agent tetrakishydroxymethylphosphorus chloride (THPC) is used to synthesize gold n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/58B82Y30/00B22F9/24B22F1/00G01N21/64G01N33/68
CPCC09K11/58C09K11/025G01N21/6428G01N21/6456G01N33/68B82Y30/00B22F9/24G01N2333/42B22F1/0553B22F1/07B22F1/054
Inventor 刘欣沙秋月胡昭宇
Owner EZHOU INST OF IND TECH HUAZHONG UNIV OF SCI & TECH
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