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Method for detecting ethylene in fruits by using chemical fluorescent probe taking bovine serum albumin as scaffold

A bovine serum albumin and chemical fluorescence technology, which is applied in the field of chemical fluorescence probe detection of ethylene content in fruits, can solve the problems of poor selectivity, easy to be disturbed by the environment, long detection time, etc., achieve high selectivity and avoid toxic The use of solvents and the effect of short detection time

Pending Publication Date: 2021-07-23
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of environmental interference, long detection time and poor selectivity in the detection process of ethylene. The present invention provides a method for detecting ethylene in fruits with a chemical fluorescent probe using bovine serum albumin as a support.

Method used

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  • Method for detecting ethylene in fruits by using chemical fluorescent probe taking bovine serum albumin as scaffold
  • Method for detecting ethylene in fruits by using chemical fluorescent probe taking bovine serum albumin as scaffold
  • Method for detecting ethylene in fruits by using chemical fluorescent probe taking bovine serum albumin as scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A kind of bovine serum albumin is the preparation method of the chemical fluorescent probe of support, and specific implementation method is as follows:

[0023] 1. Preparation of 1-vinylpyrene: Add 1.57g of potassium tert-butoxide and 5.00g of methyl triphenylphosphine bromide to 50mL of anhydrous tetrahydrofuran at room temperature, pre-mix at 250rpm, then add 1.61g of 1 -Pyrene formaldehyde was continued to react for 8h, and the solution was neutralized with 1mmol / L hydrochloric acid; then the desired product was extracted with ethyl acetate, and the crude product was purified by silica gel column chromatography and eluted with eluent, followed by rotary evaporation at 45°C to remove the solvent Afterwards, vacuum drying at 60° C. for 30 h to obtain a yellow solid, namely 1-vinylpyrene;

[0024]2. Preparation of ruthenium-based complexes: 70 mg of the first-generation Grubbs catalyst [bis(tricyclohexylphosphine)benzylidene ruthenium dichloride] and 213 mg of 1-vinylp...

Embodiment 2

[0027] The bovine serum albumin is used as the chemical fluorescent probe of the support for the analysis and detection method of ethylene in the fruit, specifically according to the following steps:

[0028] 1. Detection of ethylene standard concentration: put 100 μL of bovine serum albumin as a scaffold chemical fluorescent probe solution and 1500 μL of high-purity water into a small-mouth threaded glass sample bottle with a rubber stopper, add 3 mL of ethylene gas of different concentrations at 37 ° C Incubate for 15 minutes; record the fluorescence spectra of different concentrations of ethylene and the fluorescence intensity at 392nm at an excitation wavelength of 365nm, and analyze the content of ethylene according to the fluorescence recovery of the fluorescent probe;

[0029] 2. Detection of ethylene concentration in apple samples: put 374g apples in a 1.6L sealed tank and incubate for 10h, collect 3mL of ethylene gas at the top every 2h, and detect according to the abo...

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Abstract

The invention discloses a method for detecting ethylene in fruits by using a chemical fluorescent probe taking bovine serum albumin as a scaffold, and relates to a method for detecting ethylene in fruits. The invention aims to solve the problems of easy environmental interference, long detection time, poor selectivity and the like in the ethylene detection process. A preparation method comprises the following steps: 1, preparing 1-vinylpyrene; 2, preparing a ruthenium-based compound; and 3, preparing the chemical fluorescent probe taking bovine serum albumin as a scaffold. The use method comprises the following steps: adding ethylene gas extracted from a fruit sample into a prepared chemical fluorescent probe aqueous solution taking bovine serum albumin as a scaffold, and carrying out fluorescence detection after constant-temperature incubation. The method is simple and safe to operate, and the prepared chemical fluorescent probe taking the bovine serum albumin as the scaffold has the advantages of high selectivity, good water solubility, short detection time and the like.

Description

technical field [0001] The invention relates to an analysis method for detecting ethylene content in fruits with a chemical fluorescent probe using bovine serum albumin as a support. Background technique [0002] Ethylene is a gaseous plant hormone whose activity varies with fruit ripening and plays a vital role in many stages of plant growth. In food and agriculture, ethylene concentration has been used as a ripeness index to determine harvest time; in storage and transportation, controlling ethylene concentration can avoid deterioration of ethylene-sensitive products. At present, the methods for determining ethylene in plants are mainly gas chromatography, near-infrared absorption spectrometry, electrochemical sensor method, etc. However, these methods have problems such as high equipment cost, complicated operation and poor anti-interference ability. For example, these detection methods have been used in the published patent "An Ethylene Gas Detection System for Testing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06G01N21/64
CPCC09K11/06G01N21/6428C09K2211/185
Inventor 陈立钢吴梦牛娜
Owner NORTHEAST FORESTRY UNIVERSITY
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