Use of combined serum metabolic marker in preparation of kit for diagnosing progress of hepatopathy, kit, and method using kit to screen serum metabolic markers

A technology for metabolic markers and screening methods, which is applied in the field of combined serum metabolic markers in the preparation of kits for diagnosing the development of liver diseases, can solve problems such as difficulties in monitoring the occurrence and development of liver diseases, and achieves alleviation of difficulties in monitoring the occurrence and development of liver diseases. The effect of application value and fast screening speed

Inactive Publication Date: 2018-02-02
HANGZHOU HEALTH BANK MEDICAL LAB CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The second object of the present invention is to provide a kit for diagnosing the progress of liver disease, which can quickly diagnose the progress of liver disease in...

Method used

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  • Use of combined serum metabolic marker in preparation of kit for diagnosing progress of hepatopathy, kit, and method using kit to screen serum metabolic markers
  • Use of combined serum metabolic marker in preparation of kit for diagnosing progress of hepatopathy, kit, and method using kit to screen serum metabolic markers
  • Use of combined serum metabolic marker in preparation of kit for diagnosing progress of hepatopathy, kit, and method using kit to screen serum metabolic markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] (1) Instruments and materials:

[0078] American AB Sciex 5500 tandem mass spectrometer;

[0079] Angilent 1290 liquid chromatograph;

[0080] Agilent C18 column (2.1 x 50 mm, 1.7 μm).

[0081] (2) Drugs and reagents:

[0082] Standard substances: GCDCS, GCDCA, GDCA, GCA, TCDCA were purchased from Sigma;

[0083] The isotope internal standards of the above bile acids are sodium glycochenodeoxycholate sodium sulfate-d4 (GCDCS-d4), glycocholic acid-d4 (Glycocholic acid-d4, GCA-d4), taurochenodeoxycholic acid-d4 ( TCDCA-d4) were all purchased from American Cerilliant Company;

[0084] Formic acid: chromatographically pure (MERCK company);

[0085] Acetonitrile: chromatographically pure (MERCK company);

[0086] Ammonium bicarbonate: analytically pure purchased from Sinopharm Group (Shanghai);

[0087] Double distilled water: Thermo.

[0088] (3) Detection solution

[0089] Stock solutions of reference substances: GCDCS, GCDCA, GDCA, GCA and TCDCA were prepared int...

experiment example 1

[0115] In order to show that the method of the present invention for screening combined serum metabolic markers with the kit for diagnosing the development of liver disease has the advantages of fast screening speed, low screening cost, and the advantages of batch screening, and can well screen the combined serum in patient serum. The screening of metabolic markers effectively alleviates the difficulty in monitoring the occurrence and development of liver diseases in the existing clinical treatment of liver diseases.

[0116] Now select 374 cases of serum samples, including 234 cases of normal samples, 67 cases of acute hepatitis samples, and 73 cases of liver cancer samples. Use the screening method of the present invention to screen the combined serum metabolic markers in each serum sample. The screening method With embodiment 1.

[0117] The content of GCDCS, GCDCA, GDCA, GCA and TCDCA in the above-mentioned serum samples in the normal control group (Health), acute hepatiti...

experiment example 2

[0121] In order to show that the method of the present invention for screening combined serum metabolic markers with the kit for diagnosing the development of liver disease can well screen the combined serum metabolic markers in patient serum, thereby effectively alleviating the current problems in the clinical treatment of liver diseases. The development and monitoring of liver disease is difficult.

[0122] 135 samples are specially selected, including 45 normal samples, 42 chronic hepatitis samples, 25 liver cirrhosis samples and 23 liver cancer samples, corresponding to normal control group (Health), hepatitis group (hepatitis), liver cirrhosis group (Cirrhosis) and liver cancer group (Hepatocellular caicinoma), using the screening method of the present invention to screen the combined serum metabolic markers in the serum samples of each of the above groups, the screening method is the same as in Example 1. At the same time, the total bile acid content in each serum sample...

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Abstract

The invention provides a use of a combined serum metabolic marker in the preparation of a kit for diagnosing the progress of hepatopathy, the kit, and a method using the kit to screen serum metabolicmarkers, and relates to the technical field of serum metabolic markers. The combined serum metabolic marker is mainly composed of sodium glycochenodeoxycholate sulfate, glycochenodeoxycholic acid, glycodesoxycholic acid, glycocholic acid and taurochenodeoxycholic acid. The kit executes combined screening detection on the content of above serum metabolites in serum in order to realize assisted diagnosis of the progress of the hepatopathy of hepatopathy patients, so the difficult monitoring problem of the occurrence and development of the hepatopathy in existing clinic therapy of the hepatopathyis effectively alleviated.

Description

technical field [0001] The invention relates to the technical field of serum metabolic markers, in particular to the use of a combined serum metabolic marker in preparing a kit for diagnosing the development of liver disease, the kit and a screening method thereof. Background technique [0002] At present, there are more than 120 million hepatitis B virus carriers in my country, and the number of cases exceeds 30 million. However, only 1% of hepatitis B patients receive treatment. Among them, chronic hepatitis B is the main cause of liver cirrhosis. Liver cirrhosis is more common in clinical practice. If it cannot be effectively controlled in time, it can lead to severe complications such as ascites, upper gastrointestinal bleeding, hepatic encephalopathy, secondary infection, cancer, and even life-threatening. Therefore, judging the development process of the patient's liver disease and effectively monitoring the occurrence and development of the patient's liver disease a...

Claims

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Application Information

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IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8822
Inventor 童鸿斌叶剑
Owner HANGZHOU HEALTH BANK MEDICAL LAB CO LTD
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