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A kit, a preparation method of the kit, and a detection method of glycocholic acid in peripheral blood realized by using the kit

A technology of glycocholic acid and a kit, which is applied in the field of immunological detection, can solve the problems of inaccurate determination of glycocholic acid, cross-reaction and the like, and achieves the effects of high detection sensitivity, good stability and high detection repeatability.

Active Publication Date: 2018-10-16
杭州利安生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

Cross-reactivity is prone to occur when using antibody-based immunoassays, leading to inaccurate glycocholic acid determinations

Method used

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  • A kit, a preparation method of the kit, and a detection method of glycocholic acid in peripheral blood realized by using the kit
  • A kit, a preparation method of the kit, and a detection method of glycocholic acid in peripheral blood realized by using the kit
  • A kit, a preparation method of the kit, and a detection method of glycocholic acid in peripheral blood realized by using the kit

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Embodiment Construction

[0047] In order to make the technical means, creative features, goals and effects achieved by the present invention easy to understand, the present invention will be further described below in conjunction with specific embodiments.

[0048] The kit provided in this embodiment includes reagent R1 and reagent R2.

[0049] Wherein, the reagent R1 contains: 1 μg / mL anti-taurine monoclonal IgG antibody, 10 μg / mL anti-glycocholate taurine monoclonal antibody, 50 μg / mL G6P, 0.01% Tween-20, 0.3 mM MgCl 2 , 0.01% NaN 3 , the solvent is the Tris-HCL buffer solution of pH6.5;

[0050] The reagent R2 contains: 0.2 U / mL glycocholic acid-G6PDH conjugate, 50 ug / mL NADP, 0.1% BSA, and the solvent is Tris-HCL buffer at pH 6.5.

[0051] In addition, the kit can include standards. The standard solution can also be prepared by itself when the kit is used.

[0052] The preparation process of the standard solution is as follows: Accurately weigh 1.000 mg of sodium glycocholate (purity 97%) from...

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Abstract

The invention discloses a kit. The kit comprises a reagent R1 and a reagent R2, the reagent R1 contains a trihydroxymethyl aminomethane (Tris-HCL) buffer solution, an anti-taurine antibody, an anti-glycocholic acid antibody and glucose-6-phosphate (G6P), and the reagent R2 contains the trihydroxymethyl aminomethane (Tris-HCL) buffer solution, a glycocholic acid-G-6-PD conjugate, NADP and BSA. The invention further discloses a preparation method of the kit, particularly discloses a preparation method of the anti-taurine antibody the anti-glycocholic acid antibody and further discloses a method for detecting peripheral blood glycocholic acid through the kit. Accordingly, interference of taurocholic acid can be avoided, the detection sensitivity reaches up to 0.1 microgram per milliliter, and the advantages of high specificity and detection repeatability, good stability and the like are achieved.

Description

technical field [0001] The invention relates to the technical field of immunological detection, in particular to a kit, a preparation method of the kit, and a detection method of glycocholic acid in peripheral blood realized by using the kit. Background technique [0002] Serum glycocholic acid (Cholyglycine, CG) is a metabolite of cholesterol in liver cells and is one of the main components of bile acid. Physiological enterohepatic circulation of bile acids involved. In liver cells, cholesterol undergoes a series of enzymatic reactions to convert primary bile acids into the gallbladder through the capillaries and bile ducts, and enters the duodenum with bile to help digest food; 95% of bile acids are reabsorbed in the terminal ileum, It returns to the liver through the portal vein, and is taken up and reused by liver cells; bile acids that have not been reabsorbed form cholanic acid derivatives under the action of intestinal bacteria, which are excreted in feces, and the t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/531G01N33/577G01N21/31
CPCG01N21/31G01N33/531G01N33/577
Inventor 胡清
Owner 杭州利安生物科技有限公司
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