63 results about "Glucose 6-phosphate" patented technology

A topical skin rejuvenation preparation to relieve wrinkles, increase the skin's natural production of hyaluronic acid, reverse the lack of suppleness, hydrate from within, erase spider veins, reduce varicose veins, lighten aging dark blotches (“liver spots”/Lentigos, Senile Lentigines), decrease acne, and reduce under eye puffiness includes Glucosamine (2-amino-2-deoxy-alpha-D-glucose), a hexosamine (6 carbon amino sugar), including its derivative and precursor compounds: Glucosamine Sulfate, Glucosamine Hydrochloride, Glucose-6-Phosphate, Acetyl Glucosamine, Fructose-6-phosphate, Glucosamine-6-Phosphate, to increase production of Hyaluronic acid and collagen from Glucosamine Sulfate, its precursors and derivatives and to increase skin muscle tone by Dimethylaminoethanol (DMAE) while over coming deficiency it creates in each cell's production of PhosphatidylCholine, whose deficiency damages cell membranes, as well as mitochondrial and lysosome membranes.

The present invention relates to a method for quickly determining cAMP content or an adenylate cyclase activity in a biological sample containing non-cyclic adenine nucleotides without the use of radioactive agents.Particularly, the present invention provides a method of determining cAMP content or an adenylate cyclase activity in a biological sample containing non-cyclic adenine nucleotides selected from the group consisting of cAMP produced by endogenous adenylate cyclase, and AMP, ATP, ADP and a mixture thereof, which comprises (1) combining a biological sample with effective amounts of apyrase, adenosine deaminase and alkaline phosphatase to enzymatically remove non-cyclic adenine nucleotides other than cAMP, and glucose-6-phosphate in the sample; (2) enzymatically converting cAMP into AMP; (3) determining an amount of AMP without the use of radioactive agents, and a kit to carry out the method.

Substance productivity is improved by introducing a metabolic pathway for synthesis of acetyl-CoA or acetic acid from glucose-6-phosphate into yeast. Acetic acid productivity, acetyl-CoA productivity, and productivity of a substance made from acetyl-CoA-derived are improved by attenuating genes involved in the glycolytic system of yeast and introducing a phosphoketolase gene into the yeast.

The invention relates to a method for preparing cordyceps sinensis health-care wine by using a cordyceps militaris culture medium and belongs to the field of brewing of health-care wine. The method is characterized by comprising the following steps of: crushing the cordyceps militaris culture medium, adding rice husks, and stirring uniformly; putting in a steamer and steaming, and discharging; adding distiller's yeast into the materials, wherein the distiller's yeast is glucoamylase and yeast, dissolving the glucoamylase, adding glucose 6-phosphate, standing, adding yeast, uniformly shaking and activating, adding cool water, shaking uniformly and stirring, and uniformly scattering in the steamed materials; and feeding into a wine pool, tightly compacting, sealing, covering rice husks, naturally fermenting, discharging out of the pool, distilling and purifying to obtain raw wine, adding purified water, adjusting the alcohol degree, and putting nano cordyceps militaris powder to obtain a product. The prepared health-care wine is clear and transparent, sweet in taste and mellow, soft and tasty and refreshing; and the wine is brewed by using the cordyceps militaris culture medium, so that the wine contains a large amount of nutritional ingredients of cordyceps sinensis and has nutrient flavor of pure grain wine, and belongs to excellent health-care wine.

The invention discloses bifidobacterium bifidum and application thereof. A preservation number of the bifidobacterium bifidum B-176 is CGMCC (China General Microbiological Culture Collection Center) No.15754, the preservation date is May 14, 2018, and the preservation institution is CGMCC. A formula of a culture medium of the bifidobacterium bifidum B-176 is characterized in that the culture medium is prepared from 1.2 to 2 percent of tryptone, 0.8 to 1.5 percent of soybean peptone, 0.8 to 1.6 percent of casein peptone, 0.5 to 1 percent of yeast extract powder, 1.0 to 1.5 percent of beef extract, 1.5 to 2 percent of glucose 6-phosphate, 1 to 2 percent of lactose and 4 to 4.5 percent of inorganic salt solution. The bifidobacterium bifidum B-176 disclosed by the invention has tolerance on gastric acid and cholate and has a better inhibition function on Escherichia coli; an experiment verifies that a strain has a better synergistic growth function with other types of lactobacillus, and anapplication range of the bifidobacterium bifidum B-176 is enlarged by the discovery. The invention also discloses the application of a lactobacillus composition containing the bifidobacterium bifidumB-176 in feed for fattening pigs. The lactobacillus composition can be added in original basic ration of the growing and fattening pigs for feeding, so that the utilization rate of the feed can be increased, the death rate can be reduced, and the slaughtering days can be reduced.

The invention relates to a method for preparing inositol through enzymic catalysis. The method comprises the following steps: performing enzyme-catalyzed conversion on a polysaccharide or an oligosaccharide which takes glucose as a unit to obtain glucose-1-phosphate; performing enzyme-catalyzed conversion on the glucose-1-phosphate to obtain glucose-6-phosphate; performing enzyme-catalyzed conversion on the glucose-6-phosphate to obtain inositol-1-phosphate; performing enzyme-catalyzed conversion on the inositol-1-phosphate to obtain inositol. The invention also provides an inositol product which is prepared according to the method. According to the method, the conversion efficiency of the inositol is effectively improved; moreover, a production process is simple, pollution-free, and low in production cost.

The invention discloses a high-concentration glucose removal method for determining serum 1,5 anhydro-D-glucitol based on a pyranose oxidase method, which comprises the following steps: preparing a 2-morpholinoethanesulfonic acid (MES) buffer solution having a pH value of 6.0-9.0, wherein the MES buffer solution contains a certain amount of glucokinase (GK), glucose-6-phosphate dehydrogenase (G6PD), nicotinamide adenine dinucleotide phosphate (NADP), pyruvate kinase (PK), phosphoenolpyruvate (PEP) and adenosine triphosphate (ATP); and adding into a serum sample containing glucose (Glu), and reacting at 20-50 DEG C for 3-5 minutes, wherein the GK converts the Glu into glucose-6-phosphoric acid (G6P) in the presence of the ATP, and adenosine diphosphate (ADP) is generated at the same time; the G6P is further converted into glucose-6-phosphate lactone (G6PL) by the G6PD in the presence of the NADP+; and the ADP generated in the reaction is converted into ATP again under the action of thePK in the presence of the PEP. The glucose removal agent prepared by the method can be stably stored at 2-8 DEG C for 14 months, and can be used for a clinical biochemical autoanalyzer extensively used at present.

The invention discloses a kit. The kit comprises a reagent R1 and a reagent R2, the reagent R1 contains a trihydroxymethyl aminomethane (Tris-HCL) buffer solution, an anti-taurine antibody, an anti-glycocholic acid antibody and glucose-6-phosphate (G6P), and the reagent R2 contains the trihydroxymethyl aminomethane (Tris-HCL) buffer solution, a glycocholic acid-G-6-PD conjugate, NADP and BSA. The invention further discloses a preparation method of the kit, particularly discloses a preparation method of the anti-taurine antibody the anti-glycocholic acid antibody and further discloses a method for detecting peripheral blood glycocholic acid through the kit. Accordingly, interference of taurocholic acid can be avoided, the detection sensitivity reaches up to 0.1 microgram per milliliter, and the advantages of high specificity and detection repeatability, good stability and the like are achieved.

The present application discloses recombinant bacteria producing L-amino acid(s) its construction method and the method of producing L-amino acid(s). The recombinant bacteria producing L-amino acid(s) according to the present invention has reduced expression of the glucose-6-phosphate isomerase Pgi and improved expression of the glucose-6-phosphate dehydrogenase Zwf-OpcA than the starting bacteria, wherein: said starting bacterium is a bacterial strain which can accumulate target amino acid(s). During fermenting and culturing the recombinant bacteria according to the present invention, it is observed that the effect of improving yield can be additive and the yield of L-amino acid(s) is improved obviously. The strategy of combinational modification according to the present invention develops a new method of improving the yield of L-amino acid(s) and hence it can be applied to produce L-amino acid(s) through bacterialfermentation.

The invention discloses a method for preparing hypophosphorous acid (salt) and gluconic acid (salt) from glucose phosphate serving as a raw material, which comprises the following steps of: (1) preparing raw materials: preparing subphosphate or acid phosphate and glucose, and taking water as a solvent; (2) heating: continuously heating mixed solution at the temperature of more than or equal to 100DEG C under the air pressure of more than or equal to 0.01MPa for more than 10 minutes; (3) separating: adding a separating agent, concentrating, and separating to obtain crude products of gluconic acid salt and hypophosphorous acid salt; (4) purifying: filtering to remove precipitates to obtain gluconic acid, adding a gas or a solvent capable of reacting with the hypophosphorous acid salt into aqueous solution of the separated hypophosphorous acid salt, and filtering to remove precipitates to obtain hypophosphorous acid; (5) preparing the hypophosphorous acid salt: reacting the hypophosphorous acid with basic oxide or hydroxide to obtain the hypophosphorous acid salt; and (6) preparing the gluconic acid salt: reacting the gluconic acid with basic oxide or hydroxide to obtain the gluconicacid salt. The production process is environment-friendly and harmless, raw materials are readily available, the cost is low, the yield is high, the period is short and the safety is achieved.

The invention provides a fructan consisting of 25 beta-D-fructoses and 1 alpha-D-glucose, and a method for preparing the fructan from Hubei dwarf lilyturf tuber serving as a raw material. The fructan has obvious effect of reducing blood sugar of mice with type 2 diabetes, simultaneously improves the sugar tolerance of the mice with the type 2 diabetes, and obviously enhances the expression of insulin receptor (InsR), insulin receptor substrate (IRS-1), protein kinase and phosphatidylinositol3-kinase (PI3-K) to further accelerate the glucose to be converted into glycogen and improve the activities of glucose-6-phosphate and glucose-6-phophatase so as to obvious reduce the insulin resistance.

The invention discloses a drink for supplementing human body energy and recovering people from fatigue as well as a preparation method and application thereof. The beverage is characterized by containing glucose-6-phosphate. The preparation method comprises the following steps: mixing glucose, adenosine triphosphate (ATP) and magnesium chloride; and obtaining a mixed solution containing glucose-6-phosphate in the presence of hexokinase serving as a catalyst. Besides the glucose-6-phosphate, the beverage provided by the invention also contains a defined amount of components such as glucose, sodium ions, potassium ions, magnesium ions, and the like. The components, after being combined, can fully perform a synergistic action, and effectively perform functions of quickly supplementing human body energy, and recovering people from fatigue. Meanwhile, the beverage can supplement the potassium ions, the sodium ions and the magnesium ions, and achieve a function of maintaining acid-base balance of a human body and improving the immunity. The nutrient solution containing glucose-6-phosphate is mild, suitable for long-term consumption and free of side effects.

Isolated nucleic acid molecule, coding for a polypeptide having the amino acid sequence referred to in each case in Table 1/column 2, wherein the nucleic acid molecule in the amino acid position indicated in Table 1/column 4 encodes a proteinogenic amino acid different from the particular amino acid indicated in Table 1/column 5 in the same row.

The invention relates to a kit for determining ATP content in trace amounts of sample and application thereof. The kit comprises a solution I which is a mixture of TrisHCl and bovine serum albumin; a solution II which is a sodium hydroxide solution; a solution III which is a mixture of hydrochloric acid and the TrisHCl; a reagent I comprising the TrisHCl, the bovine serum albumin, magnesium chloride, dithiothreitol, D-glucose, nicotinamide adenine dinucleotide phosphate, hexokinase and colourless glucose-6-phosphate dehydrogenase; a reagent II comprising Imidapril HCl, the bovine serum albumin, alpha-ketoglutaric acid monosodium salt, sodium hydroxide, ammonium acetate, adenosine diphosphate, glucose-6-phosphoric acid, glutamate dehydrogenase and the colourless glucose-6-phosphate dehydrogenase; a reagent III comprising the Imidapril HCl, ethylenediaminetetraacetic acid, the magnesium chloride, the ammonium acetate, NADP and glucose 6-phosphate dehydrogenase. The kit is simple to operate, and the determination result is more accurate.

The invention discloses a kit and method for determining content of glucose 6-phosphate (G6P) based on a micro method. The kit comprises the following reagents: an extract consisting of a Tris-HCl solution, a first reagent consisting of Tris, MgCl2, water and concentrated hydrochloric acid, a second reagent consisting of glucose-6-phosphate 1-dehydrogenase (G6PDH) and a third reagent consisting ofNADP. A principle of the method is as follows: the G6PDH can catalyze the G6P and NADP+ to NADPH and an increase rate of the NADPH at 340 nm is measured to reflect level of G6P content. The used micro method is rapid and effective in test and can prevent a problem of G6P decomposition possibly caused by too long test time. A sample pretreatment process is simple and easy, besides, measurement operation steps are all automatic operations of instruments, accurate data are read, each sample has a short test time, and the kit is good in repeatability and high in accuracy.

The invention is about the enzyme method of measuring magnesium ion and its diagnosis reagent box. Making use of the peculiarity that magnesium ion in the sample of plasma or serum and so on can activate the activation of hexokinase, and producing glucose-6-phosphate by reacting glucose under the existence of adenosine triphosphate, and then causing coupled reaction with phosphoglucose dehydrogenase and transferring oxidized coenzyme to reduced coenzyme. Testing the ascending range of dominant wave-length340nm absorbance and finally measuring the content of magnesium ion in the sample. This method has high specificity and would not be contaminated by material of internal and exogenous sources, and the result is precise and accurate. Diving the diagnosis reagent box into double-dose or three-dose can reduces the cross interaction of each element, keeps the stability of the reagent and deposits chronically. Using this method can realize the fast testing in common ultraviolet/ visible light analyzer or semiautomatic/automatic analyzer and doesní»t require special or additional apparatus, so the cost is low. Thus, this method can be easily promoted and applied in the whole industry.