Beverage for supplementing human body energy and recovering people from fatigue as well as preparation method and application thereof

Abstract

The invention discloses a drink for supplementing human body energy and recovering people from fatigue as well as a preparation method and application thereof. The beverage is characterized by containing glucose-6-phosphate. The preparation method comprises the following steps: mixing glucose, adenosine triphosphate (ATP) and magnesium chloride; and obtaining a mixed solution containing glucose-6-phosphate in the presence of hexokinase serving as a catalyst. Besides the glucose-6-phosphate, the beverage provided by the invention also contains a defined amount of components such as glucose, sodium ions, potassium ions, magnesium ions, and the like. The components, after being combined, can fully perform a synergistic action, and effectively perform functions of quickly supplementing human body energy, and recovering people from fatigue. Meanwhile, the beverage can supplement the potassium ions, the sodium ions and the magnesium ions, and achieve a function of maintaining acid-base balance of a human body and improving the immunity. The nutrient solution containing glucose-6-phosphate is mild, suitable for long-term consumption and free of side effects.

Description

technical field

[0001] The invention relates to a drink, in particular to a drink containing glucose-6-phosphate and its preparation method and application. Background technique

[0002] Carbohydrates, such as starch, are the main source of energy for human life activities. After carbohydrates such as starch enter the gastrointestinal tract as food, they are first hydrolyzed by enzymes to generate glucose, which is then absorbed. After glucose enters the cell, under the catalysis of a series of enzymes, the catabolic process occurs, mainly anaerobic decomposition process, that is, glycolysis. During the glycolysis phase, glucose is catabolized into pyruvate. Then enter the tricarboxylic acid cycle, and finally decompose into CO 2 and H 2 O, and generate a lot of energy. Obviously, sugar substances (such as starch, etc.) as energy substances need to go through a relatively long process from being ingested into the body to producing energy. Especially in the initial stag...

Examples

Embodiment 1

[0013] Embodiment 1: Preparation of glucose-6-phosphate nutrient solution

[0014] (1) Preparation of glucose-6-phosphate nutrient solution by traditional enzymatic method

[0015] Add 6g of ATP, 20g of glucose and 2g of magnesium chloride in a 1000ml flask, and add 2mg of phosphohexokinase under the conditions of pH 6.5 and 38°C, and keep the reaction for 4 hours. After the reaction, filter with a 6K ultrafiltration membrane and collect the filtrate. Then ATP and ADP are recovered with Cl type anion chromatography column. Then use Na-type cation chromatography column to remove part of Mg 2+ Afterwards, after concentration, a solution containing about 5% glucose-6-phosphate and 5% glucose was obtained. Finally, add appropriate amount of KCl, NaCl, vitamin C and concentrated fruit juice for seasoning to prepare glucose-6-phosphate nutrient solution.

[0016] (2) Preparation of glucose-6-phosphate nutrient solution by solid-phase enzymatic method

[0017] The process of pre...

Embodiment 2

[0018] Embodiment 2: the animal experiment of anti-fatigue of 6-glucose phosphate nutrient solution

[0019] (1) Experimental materials:

[0020] Experimental samples: 10% glucose hexaphosphate solution, 10% glucose solution.

[0021] Experimental animals: 21 Kunming white mice (male), weighing 20±2g, and aged about 3 weeks.

[0022] Experimental equipment: swimming pool (45cm×35cm×35cm), timer

[0023] (2) Experimental method:

[0024] The mice were randomly divided into 3 groups: blank control group, glucose hexaphosphate group, and glucose group, with 7 mice in each group. Each group was given physiological saline, 10% glucose hexaphosphate solution, and 10% glucose solution by intragastric administration. Before administration, the mice in each group performed weight-bearing swimming (see below for the method) to make them physically exhausted, and then administered the drug by gavage immediately. After 30 minutes, they performed weight-bearing swimming again, and the ...

Embodiment 3

[0030] Embodiment 3: 6-glucose phosphate nutrient solution rapid recovery exercise fatigue test

[0031] (1) Test subjects: 20 postgraduates who volunteered to participate in the test participated in the test, the ratio of male to female was 10:10, and the age was 23 to 30 years old. The test time was the beginning of September 2010, and the temperature on that day was 34°C. The experimenter jogged 1000 meters in the stadium. The results showed that there were 14 people who had obvious thirst, bitter taste, irritability, dizziness and fatigue. There are 6 people with mild symptoms above.

[0032] (2) Test method: Select 14 people with obvious fatigue feeling to participate in the test. After jogging for 1000 meters, each person rested on the spot, and each person drank a bottle of 300ml beverage within 10 minutes. The participants in the experiment were divided into 3 groups, the first group consisted of 5 persons, who drank nutrient solution containing 5% glucose 6-phosph...

technical field

[0001] The invention relates to a drink, in particular to a drink containing glucose-6-phosphate and its preparation method and application. Background technique

[0002] Carbohydrates, such as starch, are the main source of energy for human life activities. After carbohydrates such as starch enter the gastrointestinal tract as food, they are first hydrolyzed by enzymes to generate glucose, which is then absorbed. After glucose enters the cell, under the catalysis of a series of enzymes, the catabolic process occurs, mainly anaerobic decomposition process, that is, glycolysis. During the glycolysis phase, glucose is catabolized into pyruvate. Then enter the tricarboxylic acid cycle, and finally decompose into CO 2 and H 2 O, and generate a lot of energy. Obviously, sugar substances (such as starch, etc.) as energy substances need to go through a relatively long process from being ingested into the body to producing energy. Especially in the initial stag...

Examples

Embodiment 1

[0013] Embodiment 1: Preparation of glucose-6-phosphate nutrient solution

[0014] (1) Preparation of glucose-6-phosphate nutrient solution by traditional enzymatic method

[0015] Add 6g of ATP, 20g of glucose and 2g of magnesium chloride in a 1000ml flask, and add 2mg of phosphohexokinase under the conditions of pH 6.5 and 38°C, and keep the reaction for 4 hours. After the reaction, filter with a 6K ultrafiltration membrane and collect the filtrate. Then ATP and ADP are recovered with Cl type anion chromatography column. Then use Na-type cation chromatography column to remove part of Mg 2+ Afterwards, after concentration, a solution containing about 5% glucose-6-phosphate and 5% glucose was obtained. Finally, add appropriate amount of KCl, NaCl, vitamin C and concentrated fruit juice for seasoning to prepare glucose-6-phosphate nutrient solution.

[0016] (2) Preparation of glucose-6-phosphate nutrient solution by solid-phase enzymatic method

[0017] The process of pre...

Embodiment 2

[0018] Embodiment 2: the animal experiment of anti-fatigue of 6-glucose phosphate nutrient solution

[0019] (1) Experimental materials:

[0020] Experimental samples: 10% glucose hexaphosphate solution, 10% glucose solution.

[0021] Experimental animals: 21 Kunming white mice (male), weighing 20±2g, and aged about 3 weeks.

[0022] Experimental equipment: swimming pool (45cm×35cm×35cm), timer

[0023] (2) Experimental method:

[0024] The mice were randomly divided into 3 groups: blank control group, glucose hexaphosphate group, and glucose group, with 7 mice in each group. Each group was given physiological saline, 10% glucose hexaphosphate solution, and 10% glucose solution by intragastric administration. Before administration, the mice in each group performed weight-bearing swimming (see below for the method) to make them physically exhausted, and then administered the drug by gavage immediately. After 30 minutes, they performed weight-bearing swimming again, and the ...

Embodiment 3

[0030] Embodiment 3: 6-glucose phosphate nutrient solution rapid recovery exercise fatigue test

[0031] (1) Test subjects: 20 postgraduates who volunteered to participate in the test participated in the test, the ratio of male to female was 10:10, and the age was 23 to 30 years old. The test time was the beginning of September 2010, and the temperature on that day was 34°C. The experimenter jogged 1000 meters in the stadium. The results showed that there were 14 people who had obvious thirst, bitter taste, irritability, dizziness and fatigue. There are 6 people with mild symptoms above.

[0032] (2) Test method: Select 14 people with obvious fatigue feeling to participate in the test. After jogging for 1000 meters, each person rested on the spot, and each person drank a bottle of 300ml beverage within 10 minutes. The participants in the experiment were divided into 3 groups, the first group consisted of 5 persons, who drank nutrient solution containing 5% glucose 6-phosph...

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