Serum-free immune cell culture medium and preparation method thereof

A technology of immune cells and culture medium, applied in the field of serum-free immune cell culture medium, can solve the problems of lack of culture medium and lack of research, and achieve the effect of increasing the positive ratio

Inactive Publication Date: 2018-01-12
万向东方生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The clinical application of DC vaccines requires the number of DCs to reach a certain order of magnitude, and there are also requirements for the proportion of phenotype-positive cells in DCs, but there is currently a lack of relevant research and no suitable culture medium

Method used

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  • Serum-free immune cell culture medium and preparation method thereof
  • Serum-free immune cell culture medium and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049]A serum-free immune cell culture medium, made into 1L of said medium mainly includes the following weight raw materials:

[0050] L-alanine 5mg, L-arginine hydrochloride 30mg, L-aspartic acid 10mg, L-aspartic acid hydrate 20mg, L-cystine hydrochloride 20mg, L-glutamic acid 10mg, L-glutamine 50mg, L-histidine hydrochloride 15mg, L-isoleucine 20mg, L-leucine 20mg, L-lysine hydrochloride 10mg, L-methionine 5mg, L-phenylalanine 5mg, L-proline 5mg, L-serine 5mg, L-threonine 10mg, L-tryptophan 1mg, L-tyrosine 10mg, L-valine 1mg, L- Glycine 1mg;

[0051] Calcium chloride 50mg, potassium chloride 250mg, magnesium sulfate heptahydrate 50mg, sodium selenite 0.001 mg, sodium chloride 4000mg, sodium dihydrogen phosphate 50mg, sodium bicarbonate 1000mg;

[0052] Biotin 0.05mg, Choline Chloride 0.1mg, D-Calcium Pantothenate 0.005mg, Folic Acid 0.05mg, Inositol 1mg, Nicotinamide 0.1mg, Riboflavin 0.1mg, Thiamine Hydrochloride 0.05mg, Vitamin B12 0.1 mg, Hydrochloric Acid Pyridoxal 0...

Embodiment 2

[0056] A serum-free immune cell culture medium, made into 1L of said medium mainly includes the following weight raw materials:

[0057] L-alanine 30mg, L-arginine hydrochloride 200mg, L-aspartic acid 40mg, L-aspartic acid hydrate 70mg, L-cystine hydrochloride 100mg, L-glutamic acid 60mg, L-glutamine 600mg, L-histidine hydrochloride 60mg, L-isoleucine 150mg, L-leucine 150mg, L-lysine hydrochloride 200mg, L-methionine 50mg, L -Phenylalanine 100mg, L-proline 100mg, L-serine 60mg, L-threonine 80mg, L-tryptophan 20mg, L-tyrosine 80mg, L-valine 100mg, L-glycine 50 mg;

[0058] Calcium chloride 200mg, potassium chloride 500mg, magnesium sulfate heptahydrate 300mg, sodium selenite 0.010 mg, sodium chloride 8000mg, sodium dihydrogen phosphate 300mg, sodium bicarbonate 5000mg;

[0059] Biotin 0.5mg, Choline Chloride 20mg, D-Calcium Pantothenate 10mg, Folic Acid 10mg, Inositol 50mg, Niacinamide 10mg, Riboflavin 0.5mg, Thiamine Hydrochloride 5mg, Vitamin B12 3.0mg, Pyridoxal Hydrochlor...

Embodiment 3

[0063] A serum-free immune cell culture medium, made into 1L of said medium mainly includes the following weight raw materials:

[0064] L-alanine 8mg, L-arginine hydrochloride 50mg, L-aspartic acid 13mg, L-aspartic acid hydrate 30mg, L-cystine hydrochloride 36mg, L-glutamic acid 14.7mg, L-glutamine 100mg, L-histidine hydrochloride 21.88mg, L-isoleucine 30mg, L-leucine 30mg, L-lysine hydrochloride 29mg, L-methionine 10mg , L-phenylalanine 15mg, L-proline 11.5mg, L-serine 10.5mg, L-threonine 16mg, L-tryptophan 3mg, L-tyrosine 18mg, L-valine 5mg , L-glycine 7mg;

[0065] Calcium chloride 60mg, potassium chloride 285mg, magnesium sulfate heptahydrate 100mg, sodium selenite 0.003mg, sodium chloride 5200mg, sodium dihydrogen phosphate 85mg, sodium bicarbonate 1800mg;

[0066] Biotin 0.11mg, Choline Chloride 0.5mg, D-Calcium Pantothenate 0.01mg, Folic Acid 0.1mg, Inositol 5mg, Niacinamide 0.5mg, Riboflavin 0.15mg, Thiamine Hydrochloride 0.1mg, Vitamin B12 0.5mg, Pyridoxal hydroch...

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Abstract

The invention discloses a serum-free immune cell culture medium. The serum-free immune cell culture medium is prepared mainly from amino acids, vitamins, minerals, D-glucose, 4-hydroxyethyl piperazinyl ethanesulfonic acid, insulin, phenol red, sodium pyruvate and transferrin. The invention discloses another serum-free immune cell culture medium which further comprises cofactors including salidroside, luteolin and resveratrol. The invention discloses another serum-free immune cell culture medium which further comprises activators including an acetyl coenzyme A, a coenzyme Q10, glucose 6-phosphate and cyclic adenosine monophosphate. A part of embodiments of the invention can increase the positive ratio of cell phenotypes.

Description

technical field [0001] The invention relates to an immune cell culture medium, in particular to a serum-free immune cell culture medium, and also relates to a preparation method thereof. Background technique [0002] Dendritic cells (Dendritic Cells, DC), named for their shape like dendrites of nerve cells, account for about 0.2% of the total number of white blood cells in the blood, but are currently considered to be the cells with the most antigen-presenting ability in the immune system. In 2011, American Bruce Beutler, Frenchman Jules Hoffmann, and Canadian Ralph Steinman for "discovering a key principle of immune system activation that revolutionized our understanding of the immune system " and won the Nobel Prize in Physiology or Medicine. Among them, Steinman contributed to the discovery of "dendritic cells" in the immune system and their role in the adaptive immune response, that is, the process of adapting to and eliminating microorganisms in the body in a self-regu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0784
Inventor 高晨
Owner 万向东方生物科技有限公司
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