A method for isolating intramuscular fat cells of Qinchuan beef cattle
A technology of intramuscular fat cells and separation method, which is applied in the field of separation of intramuscular fat cells of Qinchuan beef cattle, can solve the problem of unclear definition of the mechanism of preferential accumulation of intramuscular fat, and achieve the purpose of increasing subcutaneous and visceral fat deposition and improving muscle fat accumulation. Inner fat deposition and the effect of increasing the ratio of positive cells
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[0025] During the research process, the applicant found that more positive preadipocytes could be obtained by digesting the longissimus dorsi muscle of Qinchuan beef calf with type II collagenase, compared with other methods such as type I collagenase digestion, or with adult cattle. There are some differences in intramuscular adipocytes obtained from the longissimus dorsi samples.
[0026] The applicant found that the longissimus dorsi muscle tissue of newborn cattle (0-1 weeks after birth) was compared by using different age groups of bovine longissimus dorsi muscle tissue, different collagenase, different digestion time and different differential adhesion time. After 1.5 hours of collagenase type II digestion, more positive preadipocytes could be obtained by replacing with fresh medium 1.5 hours after seeding. At the same time, the use of immunofluorescence and other methods has further improved the reliability of the identification of intramuscular preadipocytes.
[0027]...
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