The invention discloses a method for breeding Macropodus opercularis with a transferred green fluorescent protein gene by using piggyBac transposons, which comprises the following steps of: firstly, constructing piggyBac transposons with green fluorescent protein genes and a helper plasmid capable of providing transposase; and secondly, transferring the two transposons into a germ cell of the Macropodus opercularis by using microinjection technology, and according to the transposons characteristics of the piggyBac transposons, introducing the green fluorescent protein genes into a genome of the Macropodus opercularis so as to make the gene inherited and expressed stably. An individual of transgenic Macropodus opercularis can be screened by means of the fluorescence-labeled gene, and the Macropodus opercularis with the transferred green fluorescent protein gene is more worthy of appreciation and represents a new variety of Macropodus opercularis with specific function. In addition, it is the first time for the piggyBac transposons to be used in researches on transgenic fish, and thus, the research space for transgenic aquatic animals is widened.