5results about How to "Efficient marking" patented technology

The invention discloses a method for labeling and absolute quantification of extracellular vesicles and liposomes by lanthanide metal. EVs or lipidosome and a lanthanide metal ion solution are jointly incubated, the EVs or lipidosome is marked through specific binding of lanthanide metal ions and phosphate radical groups on the surface of an EVs or lipidosome membrane, ICP-MS detection is conducted, and absolute quantification of the EVs or lipidosome is achieved. According to the method, efficient labeling of the EVs or the lipidosome can be achieved only through one-step reaction, operation is easy, convenient and rapid, good labeling stability and biocompatibility are achieved, and the physical and chemical properties and biological activity of the EVs or the lipidosome cannot be remarkably affected. The method can also be applied to absolute quantification of EVs or lipidosome ingesting by recipient cells, and provides a new research thought and technical means for research on interaction between EVs and recipient cells, cargo delivery and related mechanisms or delivery effect quantification of lipidosome drugs in cells or even in vivo.

The present invention provides a fluorescent labeling method and quantitative analysis method for gelatin protein, wherein tetrazole-modified aggregation-induced luminescence fluorescent dye molecules are dissolved in an aqueous phase, an alcohol phase or a PBS buffer solution, and then mixed with a gelatin protein solution to obtain a fluorescent protein complex solution. The tetrazole-modified aggregation-induced luminescence fluorescent dye molecules do not emit light or emit light weakly when dissolved, but after being combined with gelatin protein, the fluorescence of the dye molecules is significantly enhanced, and can be used as a fluorescent labeling and quantitative detection method for gelatin protein.

This utility model discloses an auxiliary planting tool for cherry seedlings, belonging to the field of cherry seedling planting technology. Its key technical features include a fixed frame with a marking mechanism inside, a spacing mechanism on the rear side of the fixed frame, and a lifting rod on the top of the fixed frame. The spacing mechanism includes an indicator cone, a through hole, several graduated grooves, a spacing rod, and a limiting frame. The through hole is located inside the limiting frame, the top of the indicator cone is fixedly connected to the top of the inner wall of the through hole, the spacing rod is movably inserted into the limiting frame, and the graduated grooves are located on the rear side of the spacing rod. This invention solves the problem that existing methods for spacing cherry seedlings require prolonged bending and measuring with a ruler to mark the planting position on the ground, which is inconvenient, consumes a lot of physical strength, affects efficiency, and provides limited assistance for cherry seedling planting.

The present invention provides a laser marking method based on the interface between a silicon carbide substrate and a polymer, which utilizes the photothermal conversion at the contact interface between the silicon carbide substrate and the polymer film to achieve laser induced patterning. The method provided by the present invention does not require chemical etching and physical doping, and only requires a silicon carbide substrate to achieve laser marking on the surface of a polymer film, overcoming the problem that most polymers cannot be marked due to lack of laser responsiveness, while avoiding the overall filling of laser sensitive substances in the polymer, improving the laser marking effect, and facilitating large-scale promotion and application.

An aero-engine part marking device comprises a marking device body, a part moving tool, a guide rail and a workbench, and the marking device body is arranged on the workbench; the part moving tool and the workbench are arranged adjacently, and a guide rail is arranged in a matched mode and used for guiding the part moving tool to be close to the workbench. The guiding track limits the butt joint track of the part moving tool, and the part moving tool is in butt joint with the positioning part B of the workbench through the positioning part A, so that the working area of the marking device falls on a part fixed to a rotary disc of the part moving tool. The marking device comprises a controller, a marking device body, a marking supporting rack and a cantilever. A marking pen of the marking device body is arranged towards a rotary disc of the part moving tool. The marking supporting rack is fixed to the workbench, the cantilever is arranged on the marking supporting rack and can rotate relative to the marking supporting rack, the marking device is arranged on the cantilever, and the position of the marking device on the cantilever is adjustable.