38results about How to "Efficient marking" patented technology

The invention discloses a vector for efficiently labeling zebra fish PGC, and a preparation method and a use of transgenic fish. A Gal4/UAS transcription activation system is utilized and mRFP is used as a report gene to realize an inductive gene expression regulation technology in the primordial germ cells of zebra fish. The preparation method of the transgenic fish comprises the following steps: 1, respectively constructing activation transgenic line Tg (kop:KalTA4) and an effect transgenic line Tg (UAS:mRFP); and 2, hybridizing Tg (kop:KalTA4) male fish with Tg (UAS:mRFP) female fish to obtain the transgenic fish for efficiently labeling the primordial germ cells of zebra fish. The transgenic fish for efficiently labeling the primordial germ cells of zebra fish can be widely applied to the fish bioengineering.

The invention provides a stamping device for an autoclaved aerated concrete building block. The stamping device comprises a lifting platform and a conveyer belt device, wherein the lifting platform isused for lifting the autoclaved aerated concrete building block to the conveying front end of the conveyer belt device, and the conveyer belt device is used for conveying the autoclaved aerated concrete building block to a stamping area for stamp mark; the top of the lifting platform is transversely provided with a first hydraulic telescopic rod, and the telescopic end of the first hydraulic telescopic rod is vertically connected with a first push plate; a photoelectric sensor is arranged at the conveying front end of the conveyer belt device, and the conveying rear end of the conveyer belt device is provided with a stamping device body used for carrying out stamp mark on the autoclaved aerated concrete building block; and the photoelectric sensor is electrically connected with the inputend of a controller, the output end of the controller is electrically connected with a frequency converter, and the frequency converter is electrically connected with the input end of a driving motorof the conveyer belt device. The stamping device is high in automatic operation and efficiency, capable of replacing manual stamping, and stable, accurate and efficient in stamping.

The invention discloses an event extraction and processing method for case-following electronic files. The method comprises the following steps: step 1, acquiring required file data from a case-following electronic file circulation processing platform and storing the required file data into a database; 2, constructing an event trigger word dictionary, matching an electronic file event descriptionparagraph, and performing text preprocessing methods such as sentence segmentation, word segmentation and part-of-speech tagging; 3, event attribute extraction: combining dependency syntax analysis and a semantic role labeling method to obtain six event attributes including an applicant, a susceptor, a behavior, time, a place and a mode of an event; and step 4, event aggregation: aggregating the atomic events into topic events, combining similar topic events, and storing the topic events into an event database. According to the method, the problem that crime fact information is rapidly obtained by the case-following electronic file is solved, event extraction and organization can be more accurately carried out on crime facts, and the method is a basis for efficient and high-quality paper marking.

The invention discloses a sterol-based hapten-alkaline phosphatase cross-linked product and a preparation method thereof. According to the present invention, labeling is performed with the carboxyl contained in modified sterol or a sterol-based molecule derivative, and the sterol or the sterol-based molecule derivative is labeled on alkaline phosphatase molecules by using carbodiimide and N-hydroxysuccinimide; the efficiency of the method for preparing the alkaline phosphatase-labeled sterol-based hapten is high; the activities of various components of the cross-linked product are good; the cross-linked product has high sensitivity; the method has characteristics of simple operation and short preparation period; and the sterol-based hapten-alkaline phosphatase cross-linked product is provided.

The invention discloses a method for integrally marking drosophila proteomes by using stable isotope labeling with amino acids in cell culture (SILAC) and a special culture medium. The special culture medium for marking drosophila by using the SILAC is prepared according to the following method: a saccharomycete body marked by using the SILAC is coated on a drosophila culture medium to obtain the special culture medium for marking the drosophila by using the SILAC. An experiment proves that the culture medium for marking the drosophila by using the SILAC can be specially used for marking the drosophila proteomes by using the SILAC, can achieve rapid and high-efficient marking, and creates conditions for preparation of quantified interior labels and high-precision quantified proteome studying.

Thermostable Cas9 nucleases. The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides an isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith. The Cas protein or polypeptide is capable of binding, cleaving, marking or modifying a double stranded target polynucleotide at a temperature in the range 20° C. and 100° C. inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The invention also provides PAM sequences recognized by the Cas protein or polypeptide, The Cas9 nucleases disclosed herein provide novel tools for genetic engineering in general, in particular at elevated temperatures.

The invention provides a device for marking the axis center point of a pipeline bent section. The device comprises a machining piece A, a machining piece B, a machining piece C, a machining piece D and a pulling wire; the lengths of the machining piece A and the machining piece B are the same, and the lengths of the machining piece C and the machining piece D are the same; one end of the machining piece A is movably connected with one end of the machining piece B, and the other end of the machining piece A is movably connected with one end of the machining piece D; the other end of the machining piece D is movably connected with one end of the machining piece C, and the other end of the machining piece C is movably connected with the other end of the machining piece B; and one end of the pulling wire is fixed to the connecting position of the machining piece C and the machining piece D, and the other end of the pulling wire is the free end. According to the device, the characteristic that the straight line where the diagonal line of a pair of deltoid unequal angles is located serves as a symmetric axis is utilized, efficient and accurate marking of the axis center point of the pipeline bent section is achieved, and the problem that the mode that the center line of the pipeline bent section is marked by personnel is low in efficiency and poor in accuracy is solved; and particularly through the wire pulling mode, the axis center point of the pipeline bent section can be accurately marked on pipelines with different diameters.

The invention provides a defect detection wafer graph optimization method and an optimization system thereof. The defect detection wafer graph optimization method comprises the following steps of: performing defect detection on a wafer through a machine end to obtain a wafer graph; carrying out probe testing on each chip on the wafer, and importing probe testing data of the chips into the machine end; generating a wafer probing map according to the probing data of each chip; and making the proportion and coordinates of the wafer prober map and the wafer map consistent so as to compare the wafer prober map and the wafer map, and marking chips which are not in the wafer prober map in the wafer map. According to the defect detection wafer graph optimization method, the prober data is imported into the machine end, the wafer prober map is generated, the wafer prober map is compared with the wafer map of the machine end, invalid chips can be marked efficiently and accurately, optimization of the wafer map is achieved, the capacity of the machine is released, time is saved, and manpower consumption is reduced.

The invention discloses a multifunctional tumor injector. The multifunctional tumor injector comprises an injector body and a puncture needle body. An injector cylinder is arranged in the injector body. A puncture needle is arranged in the puncture needle body. A puncture needle sleeve is connected to the outer side of the puncture needle detachably. A first puncture needle barb is arranged on thelower portion of the puncture needle. A second puncture needle barb is arranged on the upper portion of the puncture needle. A puncture needle through hole is formed in the lower portion of the puncture needle. A puncture needle in-vitro marker is arranged at the top of the puncture needle. The multifunctional tumor injector has the advantages that the injector body and the puncture needle are arranged, and the puncture needle is connected with the injector body flexibly through a fastener, so that the position of a tumor of a patient can be marked and fastened by multiple angles; the markingbarb and the auxiliary fastening barb are arranged, so that the tumor can be marked and fastened efficiently; through the through hole and a flap capable of being opened in a unidirectional way, marking liquor can be supplemented at any time, so that the capability of efficient tumor marking is guaranteed.

The invention discloses an 89Zr drug labeling and purifying device. The device comprises a pressure device, a to-be-labeled drug solution raw material bottle, a chelating agent solution raw material bottle, an 89Zr solution raw material bottle, a product bottle, two reaction kettles, two purification columns, two buffer solution raw material bottles, at least five three-way valves and at least two one-way valves. The 89Zr drug labeling and purifying device provided by the invention can be automatically controlled, is simple to operate, is safe and convenient, and can effectively reduce the ionizing radiation exposure of operators; the labeling process and the purification process can be carried out in the device, isotope pollution is not prone to occurring, and 89Zr medicine labeling and purification can be efficiently, rapidly and accurately completed; the device provided by the invention is used for labeling and purifying the 89Zr medicine, the operation is easy, the marking can be efficiently and quickly completed, and the radiochemical purity of the marked medicine can reach 95% or above.

The invention belongs to the technical field of isotopic tracing, and relates to the field of basic research of algae, in particular to a method for efficiently extracting an isotopic labeled chlorella soluble extracellular polymeric substance. Comprising the following steps: preparing a culture medium; culturing chlorella to obtain biomass; extracting an S-EPS concentrated solution and a B-EPS concentrated solution; enriching and concentrating the chlorella isotope labeled extracellular polymeric substance; performing dialysis; and cooling and drying to obtain chlorella isotope labeled extracellular polymeric powder. According to the method, efficient and rapid labeling of the chlorella extracellular polymeric substance is realized, the use of isotopes is saved, and the cost is saved. The rapid concentration of the efficiently labeled soluble extracellular polymeric substance is realized, the workload of subsequent dialysis is reduced, and the extraction efficiency is improved.

The invention discloses a marker acting on a human epidermis and used for carrying out marking before radiotherapy. The marker comprises a shell and a pen point. The marker comprises the handheld shell and the pen point, wherein a solution box is mounted in the shell, a gentian violet solution is contained in the solution box, a valve plate is slidably connected in the solution box, the valve plate is fixedly mounted at the axis of the shell through a vertical rod, and a pressing plate slides downwards to drive the solution box to slide downwards. According to the marker acting on the human epidermis and used for carrying out marking before the radiotherapy, a supply structure of a dyeing solution is redesigned, the pen point in the marker can stably discharge liquid to achieve a marking purpose in a one-time-pressing continuous liquid-supply mode, and the purpose of direct marking without contacting the skin of a patient can be achieved by taking the same pressing acting force as a driving force, so that suffering increase caused by direct contact between the skin and a marking structure after the skin is damaged by the radiotherapy is avoided, and the marking effect is clearer and more complete.

The invention discloses a binding protein for labeling beta-D glucan and a purifying and synthesizing method of the binding protein. The purifying and synthesizing method of the binding protein comprises the steps of transformation of a vector system and optimized induction to an expression strain, screening and culturing on the high-expression strain after a small amount of tests, and a large amount of protein purification, synthesis and elution. The constructed expression vector is transformed into the expression strain, bacterial colonies are screened and PCR sequencing verification is carried out to obtain a positive strain, after a small amount of preliminary experiments are carried out, SDS-PAGE is carried out meanwhile to confirm expression of a target protein and screen out the high-expression strain, then a large number of high-expression strains are cultured, and a large amount of protein purification is further carried out. The binding protein and beta-D glucan as a fungal cell wall can be subjected to immunoaffinity effectively, and fungal surface beta-D glucan antigen is labeled accurately.

The invention discloses a bottom surface appearance inspector. The bottom surface appearance inspector comprises an inspection frame, wherein a transmission mechanism is arranged on two sides of the inspection frame; a guide rail is arranged at one end of the inspection frame and is sleeved with an identification mechanism; a shooting device is arranged just under the inspection frame. The bottom surface appearance inspector disclosed by the invention has the characteristics of simple structure and convenience in maintenance, can be used for efficiently and quickly inspecting the bottom surface of a product, can quickly carry out marking, and is in accordance with an industrial assembly line production process.

The invention discloses a mall data statistical analysis system for electronic commerce, which comprises a box body, a box door, a filter screen and a central processing unit, a ventilation net I is arranged on one side wall of the box body, and a ventilation net II is arranged on the side wall, corresponding to the ventilation net I, of the box body. The system has the beneficial effects that through the design of a data sales situation trend summarizing module, a product sales and life cycle module, a member level and national sales distribution multi-angle sales analysis module, a keyword operation analysis module and a mall page access click data analysis module, the data analysis accuracy is improved; the data statistical analysis system can have the functions of efficiently collecting relevant data and summarizing relevant important parameter indexes, the working efficiency of the system is improved, the data statistical analysis system can have the rapid heat dissipation function through the design of the first ventilation net and the second ventilation net, the temperature in the system can be kept stable during long-time work. Therefore, the working and connecting states of all parts are prevented from being influenced.

The invention relates to the technical field of material synthesis, in particular to an aggregation-induced emission material and a preparation method and application thereof.The aggregation-induced emission material comprises a metabolic group and an aggregation-induced emission group which are connected through chemical bonds, the metabolic group comprises a D-alanine derivative containing a first unsaturated bond, and the aggregation-induced emission group comprises a D-alanine derivative containing a second unsaturated bond. The aggregation-induced emission group comprises a 4-(2-(4-diphenylmethylamine phenyl ethylene) pyridine bromide derivative containing a second unsaturated bond, rapid labeling and in-situ removal of bacteria are realized, and the aggregation-induced emission group has the characteristics of rapid detection, broad-spectrum antibacterial property, drug resistance reduction and the like, and has a wide application prospect in clinical treatment of bacteria, especially drug-resistant bacteria.