25 results about "Pre adipocytes" patented technology

The application provides a kind of regulation IGFBP5 The application belongs to the technical field of gene regulation, and provides an enhancer for regulating the proliferation and adipogenic differentiation of chicken intramuscular preadipocytes, which includes one or more of enhancer EN1, enhancer EN2 and enhancer EN3, the nucleotide sequence of the enhancer EN1 is shown as SEQ ID NO. 2; the nucleotide sequence of the enhancer EN2 is shown as SEQ ID NO. 1; and the nucleotide sequence of the enhancer EN3 is shown as SEQ ID NO. 3. The enhancer can significantly enhance IGFBP5 gene expression and promote the proliferation and adipogenic differentiation of chicken intramuscular preadipocytes.

The present invention relates to a novel cyclopeptide and uses thereof. It was confirmed that when preadipocytes were treated with the novel cyclopeptide according to the present invention, the fat content was significantly reduced compared to differentiation-induced preadipocytes. Therefore, a food composition, a health functional food, a pharmaceutical composition, a quasi-drug composition, a cosmetic composition, a feed composition, and a feed additive composition comprising the cyclopeptide as an active ingredient can be usefully used for preventing, alleviating, or treating obesity.

The present invention relates to a composition comprising a Salvia miltiorrhiza or Paeonia lactiflora extract as an active ingredient for prevention or treatment of lipid metabolism disorder and, more particularly, provides a pharmaceutical composition and a health functional food composition, each comprising a Salvia miltiorrhiza extract, a Paeonia lactiflora extract, or an extract from a mixture of Salvia miltiorrhiza and Paeonia lactiflora as an active ingredient for prevention or treatment of lipid metabolism disorder. The composition prevents the differentiation of preadipocyte cells to adipocyte cells, reduces blood triglyceride and cholesterol levels, and effectively suppresses fatty liver and visceral fat formation and as such, can be advantageously used as a pharmaceutical and food composition for prevention, alleviation, or treatment of lipid metabolism disorder.

This invention discloses an enhancer that regulates chicken PGC-1α gene expression and its applications, belonging to the field of bioengineering. The nucleotide sequence of the enhancer is shown in SEQ ID NO: 1. This invention further discloses biomaterials containing this enhancer and a method for preparing cell lines with the enhancer knocked out. A dual-luciferase reporter gene system confirmed its significant activity in DF1 and ICP2 cells. Knocking out this enhancer downregulates PGC-1α gene expression in chicken preadipocytes, significantly promoting cell differentiation and lipid droplet formation. This enhancer can be used to prepare biological agents that regulate PGC-1α gene expression levels and chicken preadipocyte differentiation. It can also be applied to the genetic improvement of chicken fat traits and to the preparation of models for studying related gene function and cell differentiation mechanisms. This invention provides new targets and technical support for improving chicken fat traits.

The use of a berberine derivative NBD-125 in preparation of a drug for preventing and / or treating obesity is provided, belonging to the technical field of obesity treatment. Use of a berberine derivative NBD-125 in preparation of a drug for preventing and / or treating obesity is provided. The berberine derivative NBD-125 can prevent and / or treat obesity, with an effect significantly better than that of berberine. Moreover, the berberine derivative NBD-125 can inhibit weight gain, enhance metabolism and / or heat production, inhibit white adipose production, and inhibit differentiation of a preadipocyte into an adipocyte and / or lipid accumulation, where each inhibition index is better than that of the berberine.

The invention discloses an application of rifampicin in preparation of a medicine for regulating lipid metabolism. The invention discloses a new medical application of rifampicin by using a mouse model fed by 3T3-L1 fat cells and high fat diet (HFD). Rifampicin can increase the expression of Sirt6 in vivo and in vitro. In addition, rifampicin effectively blocks differentiation of 3T3-L1 preadipocytes and reduces lipid accumulation by inhibiting lipid generation in adipocytes and enhancing lipid decomposition. In the mechanism level, rifampicin significantly inhibits the expression of adipogenesis genes and proteins, and enhances the expression of genes and proteins of lipolysis, energy consumption and white adipose tissue browning related pathways. In conclusion, data of the invention shows that rifampicin is a potential new treatment strategy for regulating lipid metabolism.

This invention proposes a SNP marker for promoting preadipocyte differentiation in chickens and identifying broilers with high abdominal fat percentage, and its application, belonging to the field of animal molecular genetics. It includes an SNP marker for promoting preadipocyte differentiation in chickens and identifying broilers with high abdominal fat percentage. The SNP marker uses the sequence shown in SEQ ID NO.1 as its starting sequence, with position 51 being either A or G. If the SNP is of the GG genotype, it indicates a broiler with high abdominal fat percentage. Replacing A with G at position 51 promotes preadipocyte differentiation in chickens. It is mainly used to promote preadipocyte differentiation in chickens and identify broilers with high abdominal fat percentage.

The invention belongs to the technical field of cosmetics, and particularly relates to cyclic peptide for promoting adipocyte generation and application of the cyclic peptide in cosmetics. The structure of the cyclic peptide is shown as a formula (I) or (II). The cyclic peptide provided by the invention can promote the preadipocytes to differentiate into mature adipocytes, and obviously up-regulate the expression level of key adipogenesis genes such as PPAR gamma, C / EBP alpha and FABP4 in the preadipocytes, so that the cyclic peptide can be used for preventing and improving the problems of skin aging and the like caused by fat loss. (I) (II)

The invention discloses application of a transcription factor E2F5 for regulating and controlling chicken fat formation, and relates to the field of animal molecular heredity and developmental biology. Differentiation of chicken preadipocytes is inhibited through overexpression of the transcription factor E2F5, differentiation of the chicken preadipocytes is promoted through interference of the E2F5, and the transcription factor KLF7 plays a regulating role by regulating the enhancer activity of the transcription factor E2F5.

The invention discloses a method for rapidly separating and culturing preadipocytes in carp muscle and inducing adipogenesis, and relates to a method for rapidly separating and culturing fish cells and inducing adipogenesis. The isolated culture comprises the following steps: 1, muscular tissue acquisition; 2, digesting muscular tissues; 3, purifying the preadipocytes by Ficoll; 4, counting the cell suspension; and 5, purifying by adopting a differential adhesion method. And fat formation induction: performing induction by using a fat formation induction culture medium, and successfully performing fat formation induction on the proadipocytes in the carp muscle. The invention provides a method for jointly digesting muscular tissues through combination of pancreatin and collagenase, and fish intramuscular preadipocytes with high purity and large quantity are effectively obtained. The carp intramuscular preadipocytes obtained by the invention are rapid and efficient in adipogenesis, and a basic material is provided for constructing a carp intramuscular fatty degeneration model.

The invention discloses an enhancer for regulating and controlling chicken PGC-1alpha gene expression and application of the enhancer, and belongs to the field of bioengineering. The nucleotide sequence of the enhancer is shown as SEQ ID NO: 1. The invention further discloses a biological material containing the enhancer and a preparation method of a cell strain with the enhancer knocked out. A dual-luciferase reporter gene system proves that the enhancer has remarkable activity in DF1 and ICP2 cells, the expression of the PGC-1alpha gene in chicken preadipocytes can be reduced by knocking out the enhancer, and cell differentiation and lipid droplet generation are remarkably promoted. The enhancer can be used for preparing a biological preparation for regulating and controlling the expression level of the PGC-1alpha gene and regulating and controlling chicken preadipocyte differentiation, can also be applied to genetic improvement of chicken fat traits and can be used for preparing a model for researching related gene functions and cell differentiation mechanisms. The invention provides a new target and technical support for chicken fat character improvement.

The invention belongs to the technical field of cell engineering and genetic engineering, and particularly relates to application of a Dickkopf-3 (DKK3) gene in improving beef quality and a method. The DKK3 gene is applied to improvement of beef quality, the intramuscular fat content of beef can be changed, and the application of the DKK3 gene to preparation of products for promoting differentiation of beef cattle intramuscular preadipocytes is further provided. The result of the embodiment shows that the DKK3 has a promoting effect on 3T3-L1 adipogenic differentiation, and after the DKK3 is over-expressed in the preadipocytes in the primary bovine muscle, the effects of increasing lipid droplet aggregation and remarkably increasing the expression quantity of adipogenic differentiation marker genes C / EBP alpha, C / EBP beta and PPAR gamma are achieved, so that the DKK3 has an important regulating effect on the adipogenic differentiation of the preadipocytes and can be used for preparing a medicine for treating the adipogenic differentiation of the preadipocytes. And a new gene resource is provided for improving the variety of high-quality beef cattle and improving the beef quality.

The invention provides a chicken adipogenesis gene 3 '-UTR variant and application thereof, and belongs to the technical field of molecular biology, and chicken adipogenesis genes comprise BMP2 and PDK1. The invention finds that adipogenesis genes BMP2 and PDK1 have different 3 '-UTR long and short transcript expressions in the chicken preadipocyte differentiation process and the association between the dynamic change of the genes BMP2 and PDK13'-UTR and adipogenesis for the first time, and further provides the application of the 3 '-UTR variants of the genes BMP2 and PDK1 in preadipocyte differentiation identification and analysis. The invention provides a material and a method for genetic breeding of broiler chickens, and has a good application prospect in improvement of chicken quality breeding.

The present disclosure concerns methods and systems for the production of hybrid cell based meat from bio-inks. The bio-inks are cell based to provide layers of protein and connective tissue within the cell based meat. The protein is prepare by culturing muscle cells in a filamentous microcarrier while the connective tissue by culturing pre-adipocytes in polyanionic microcapsules. The bio-inks are prepare by combining the cultured cells with hydrogels.

The present invention belongs to the field of medicine / product technology, and relates to a glutinous rice fragrance extract, a preparation method and application thereof. The method comprises the following steps: the dried above-ground part of glutinous rice fragrance is crushed, and then an ethanol aqueous solution with a mass fraction of 95% is added and extracted at room temperature for 3 times, and the extraction time is 8 days, 7 days, and 6 days respectively, and the weight ratio of the ethanol aqueous solution added to the glutinous rice fragrance is 12 L: 1 kg each time, and the extracts of each time are combined and concentrated under reduced pressure to obtain an extract of glutinous rice fragrance, which is glutinous rice fragrance extract A, and the extract is extracted with ethyl acetate, and the extracts B and C are obtained by chromatography on D101 macroporous resin and MCI cation exchange chromatography column. Glutinous rice fragrance extract A-C significantly inhibits the synthesis of triglycerides in pre-adipocytes, has good anti-obesity efficacy in a high-fat diet-induced obese mouse model, improves glucose tolerance damage caused by a high-fat diet, has no toxic side effects, and has a simple preparation process.

The present invention relates to a method for the cultivation of adipose cells, which method comprises: culturing adipose cells in a culture medium; and harvesting the adipose cells, wherein prior to harvesting, the adipose cells are aggregated in the culture medium. Cells obtained from the method may be incorporated into food products.

The application discloses a uORF element for regulating chicken insulin receptor expression and application thereof, belongs to the field of molecular biology and genetic engineering, and the nucleotide sequence of the uORF element is shown in any one of SEQ ID NO. 1-2; the application comprises any one of the following: (1) regulating the expression of firefly luciferase in eukaryotic cells; the eukaryotic cells are chicken embryo fibroblasts and chicken preadipocytes; (2) being applied to genetic breeding of chickens, and the genetic breeding is breeding of meat chickens with reduced insulin resistance; (3) application in preparation of a reagent for regulating the expression of chicken insulin receptors. The application can regulate the expression level of INSR, improve insulin sensitivity, and provide material support for research on the mechanism of insulin in regulating blood glucose.

An Lrig inhibitor or Lrig expression inhibitor according to the present invention specifically binds to an Lrig protein present on preadipocytes, adipocytes, or regulatory T cells to regulate the function of the regulatory T cells, thereby regulating the activity of effector T cells, and thus can be very efficiently used for the prevention, amelioration, or treatment of metabolic diseases.

The invention discloses a method for screening fat key genes through whole genome knockout, and relates to the technical field of biology. According to the invention, a whole genome knockout system suitable for chicken preadipocyte lines (ICP) is established based on a strategy of improving lentivirus infection efficiency by a cationic co-infection agent, and lipid droplet phenotype screening is carried out in combination with flow cytometry SSC signals, so that key genes influencing fat deposition can be efficiently and systematically screened; and a new technical platform is provided for poultry fat metabolism research and low-fat breeding. Functional verification is performed on the CYP46A1 gene screened based on the system, and results show that after the gene is knocked out, lipid droplet accumulation of chicken preadipocytes is remarkably reduced, expression of lipid metabolism key genes is reduced, and the effect of the CYP46A1 gene in fat deposition regulation is defined.

The present invention discloses a substance for inhibiting the KCNK3 gene and its application in regulating fat metabolism or ferroptosis. The present invention uses the CRISPR / Cas9 technology to construct gene knockout and overexpression cell lines, and finds that the knockout of KCNK3 inhibits lipid synthesis and accumulation in porcine preadipocytes and promotes lipolysis thermogenesis. Further, by using the technology of combined transcriptome and lipidome analysis, the KCNK3 gene knockout cells are analyzed, and it is found that KCNK3 regulates the biological processes of fat metabolism and ferroptosis. By detecting the iron content, ROS level, glutathione level, mitochondrial morphological characteristics, etc. in KCNK3 knockout PK15 cells, it is proved that the knockout of KCNK3 promotes ferroptosis. The present invention discovers for the first time the role of the KCNK3 gene in promoting porcine fat formation and inhibiting ferroptosis, and this discovery has important significance for regulating porcine fat deposition by using the KCNK3 gene and for the treatment of human obesity and ferroptosis-related diseases.

The present invention relates to a novel triazole derivative or a salt thereof. The triazole derivative or the salt thereof according to the present invention induces or promotes browning of white adipocytes, and differentiates stem cells, embryonic cells, or preadipocytes to brown adipocytes or beige adipocytes. A composition comprising the triazole derivative or the salt thereof according to the present invention can treat, prevent, or alleviate obesity, obesity complications, diabetes, dyslipidemia, fatty liver, hypertension, metabolic syndrome, and insulin resistance syndrome.

The invention relates to the field of animal cell culture and improved breeding of animal husbandry, and provides an additive and a culture medium for promoting adipocyte dedifferentiation and a method for preparing dedifferentiated adipocytes, a composition of TGF-beta and naringenin is added into the culture medium to form the adipocyte dedifferentiation culture medium; during culture, a ceiling method is adopted, the fat cells are inversely cultured by a culture medium containing 20% of FBS, and a culture medium for promoting the fat cells to dedifferentiate is changed after the culture medium is corrected. According to the method, the dedifferentiation time of the bovine adipocytes is shortened from 10 days to 5 days, the dedifferentiation rate reaches 90.42%, the obtained preadipocytes can be continuously cultured and redifferentiated, and a feasible scheme is provided for large-scale production of cell culture fat.

The invention discloses a chicken KLF3 gene promoter as well as a construction method and application thereof, and belongs to the field of molecular biology and gene engineering. The nucleotide sequence of the chicken KLF3 gene promoter is any one of KLF3-A-KLF3-D sequences as shown in SEQ ID NO. 1 to SEQ ID NO. 4. The invention further discloses a preparation method of the chicken KLF3 gene promoter. It is found through tests that the promoters KLF3-A-KLF3-D have obvious promoter activity in a human embryo kidney cell 293T and a chicken preadipocyte ICP1, and the activity of the promoters KLF3-A-KLF3-D is regulated and controlled by a specific transcription factor KLF2. The KLF3 promoter provided by the invention has application value for revealing a regulation mechanism of chicken KLF3 transcription expression, verifying a high-quality broiler breeding scheme of a targeted chicken KLF3 expression level and developing a new eukaryotic protein expression system, and can also be used for screening drugs of targeted KLF3 expression at a cellular level.

The invention discloses a uORF element for regulating and controlling expression of a chicken insulin receptor and application of the uORF element, and belongs to the field of molecular biology and genetic engineering, and the nucleotide sequence of the uORF element is shown as any one of SEQ ID NO.1-2; the application comprises any one of the following applications: (1) regulating and controlling expression of firefly luciferase in eukaryotic cells; the eukaryotic cells are chicken embryo fibroblasts and chicken preadipocytes; (2) the method is applied to genetic breeding of chickens, and the genetic breeding refers to breeding of broiler chickens capable of reducing insulin resistance; and (3) application in preparation of a reagent for regulating expression of the chicken insulin receptor. The expression level of INSR can be regulated and controlled, insulin sensitivity is improved, and material support is provided for research on analysis of a mechanism for regulating and controlling blood sugar by insulin.

The invention belongs to the technical field of biology, and particularly relates to a circular RNA related to chicken intramuscular fat deposition, a ceRNA regulation network and application. According to the application disclosed by the invention, circSMARCD3 playing a key role in intramuscular fat deposition in a chicken embryonic period and a ceRNA regulation axis of the circSMARCD3 are screened and identified through a high-throughput sequencing technology, the cyclization stability of the circSMARCD3 is verified, the circSMARCD3 can be directly combined with miR-2131-5p, and the miR-2131-5p can directly target 3 'UTR regions of SORT1 and PDE4B genes and inhibit expression of the SORT1 and PDE4B genes, so that proliferation of preadipocytes in chicken embryonic period is inhibited, and the application of the circSMARCD3 in chicken embryonic period intramuscular fat deposition in chicken embryonic period intramuscular fat deposition in chicken embryonic period intramuscular fat deposition is promoted. The chicken feed additive can promote fat droplet aggregation to be remarkably increased, TG and GL contents to be remarkably increased, preadipocyte differentiation in chicken muscles is promoted, fat deposition is promoted, and the chicken quality is improved.