56 results about "Pre adipocytes" patented technology

The present invention relates generally to a tissue preparation including tissue cells and extracts thereof useful for promoting or facilitating the growth, development and differentiation of cells and tissues. More particularly, the present invention provides muscle-derived material comprising intact or extracted extracellular matrix and/or cells as well as cytokines, growth factors and other components. The muscle preparations of the present invention resemble basement membrane and are derived from cellular-based material. The muscle preparation may be used in vitro or in vivo as inter alia, a cellular scaffold in various tissue engineering applications and in other cell culture systems for nurturing and enriching a range of cell types including, but not limited to, precursor and stem cells such as pre-adipogenic cells. The muscle preparation is also useful as a base for creams, such as in the cosmetic and topical therapeutic industries and as a matrix or additive in the food industry.

Methods for inducing or accelerating a healing process of a damaged skin or skin wounds are described. The methods include administering to the skin cells colonizing the damaged skin or skin wound a therapeutically effective amount of an adipokine, an adipocyte or preadipocyte modulator, adipocytes, preadipocytes, or stem cells, or transforming the skin cells colonizing the damaged skin or skin wound such as to express and secrete an adipokine, thereby inducing or accelerating the healing process of the damaged skin or skin wound.

The present invention is directed to methods and agents for modulating the differentiation potential and/or proliferation of preadipocytes. More particularly, the present invention discloses methods and agents for modulating a fibroblast growth factor (FGF) signaling pathway, especially the FGF-1 or FGF-2 signaling pathway, for treating or preventing adiposity-related conditions including, but not limited to, obesity, lipoma, lipomatosis, cachexia or lipodystrophy or the loss of adipose tissue in trauma or atrophic conditions.

The present invention relates to preadipocyte strains that maintain replicative potential and adipogenic capacity. In particular, the invention relates to preadipocytes engineered to express telomerase reverse transcriptase (TERT). Use of the cells as research tools, in screening assays, and as therapeutic and/or clinical reagents is also described.

The subject invention pertains to supercritical carbon dioxide extracts of Commiphora mukul resin (guggul), which can be modified or not modified by some ethanol addition; methods for their production; and methods of use, such as inhibiting HMG-CoA reductase, inhibiting transformation of pre-adipocytes to adipocytes, inhibiting triglyceride storage, promoting insulin sensitivity in adipocytes, treatment of disorders (for example, hypercholesterolemia, hyperlipidemia, hyperglycemia, obesity, metabolic syndrome, cardiovascular disease, atherosclerotic heart disease, autoimmune disorder, insulin resistance, leptin resistance, arthritis, cell proliferation disorder, such as cancer and atherosclerosis; damaged skin, sores, cuts, rashes, bruises, dryness, burns, sunburn, radiation burn, and infection), and regulating or suppressing appetite.

The invention discloses a method for culturing and inducing tilapia mossambica peritoneal preadipocytes. The method comprises the following steps: a) resuspending cells by using a DMEM/F12 culture medium with 10%-15% of fetal calf serum, inoculating the cells into a cell plate, and culturing for 7 days at the temperature of 20-30 DEG C under the condition of 5% CO2; and b) replacing an induction culture medium for sequentially culturing, after inducing for 7-14 days, carrying out oil red O staining of the cells, wherein the fuzzy cell membrane edges can be seen under the microscope, and the interiors of the cells are stained to be red lipid droplets. The invention also discloses a passage method of tilapia mossambica peritoneal preadipocytes. A proliferation culture medium is used for culturing the preadipocytes for 14-20 days, then the preadipocytes are inoculated according to 2 *10<5> to 3*10<5> per milliliter, and the cells can overgrow on the cell plate after the cells are cultured for 2-3 days. The method is capable of successfully carrying out in-vitro culture, induction and passage of the tilapia mossambica peritoneal preadipocytes; the technical reference is provided for the research of the tilapia mossambica lipid metabolism.

The invention provides wine-steamed Chinese goldthread as well as a preparation method and application thereof. The wine-steamed Chinese goldthread as an insulin sensitizer and a PPAR gamma activator has the functions of inhibiting the differentiation of 3T3-L1 pre-adipocytes, improving the utilization rate of glucose of the 3T3-L1 pre-adipocytes, reducing blood fat, improving insulin resistance and treating diabetes mellitus. The wine-steamed Chinese goldthread and extracts thereof have the functions of inhibiting the differentiation of the 3T3-L1 pre-adipocytes, improving the utilization rate of the glucose of the 3T3-L1 pre-adipocytes and reducing the blood fat and the application in improving insulin resistance or treating diabetes mellitus, have a better specific effect than that of berberine hydrochloride and crude Chinese goldthread as well as extracts thereof, and the safety obviously higher than that of the crude Chinese goldthread.

Using electroporation, it is possible to activate the natural reprogramming potential of living Xenopus laevis oocytes and pass it on to donor cells placed with eggs in one electroporation chamber. We demonstrated that co-electroporation at 150 v/cm/25 μF of mature oocytes with ^˜10⁵ cells/ml of suspension of various normal and cancerous human cell lines, such as bone marrow stromal cells, foreskin fibroblasts, pre-adipocytes, CD4+ T-lymphocytes, cheek cells, cervical carcinoma (HeLa) cells and breast adenocarcinoma (MCF-7) cells, reprograms donor cells into iPSc-like cells, which form colonies on irradiated MEF feeders. The iPSc-like cells generated by this study resemble human embryonic stem cells in colony morphology and expression of stem cell-associated transcription factors, including Oct3/4, Nanog, SOX-2, Rex-1, TRA-1-60 and SSEA-1. New method obviates the use of retroviral or lentiviral gene delivery vectors and other “non-parental” reprogramming approaches.

The invention features methods of obtaining high-yield, essentially pure human predipocyte cultures. Cultures obtained according to the instant methodology are also featured as are methods of identifying adipogenic modulatory agents, e.g., high-throughput screening assays.

A method for the identification of a composition useful in the treatment of an overweight or obese person to reduce the person's body mass index is provided which comprises obtaining an extract of an ethnobotanical plant, and evaluating the activity of the extract in an assay selected from the group consisting of a lipolysis assay, an assay that measures the amount of glycerol introduced by a cell into a suspension medium of the cell, an adipocyte differentiation assay, an assay that measures the level of the enzyme glycerol-3-phosphate dehydrogenase, an assay that measures the inhibition of differentiation of preadipocytes to adipocytes, an assay that measures the accumulation of lipid in an adipocyte, an assay that measures the de-differentiation of adipocytes into preadipocytes, and combinations thereof. Sclareol and sclareol-like compounds and sclareolide and sclareolide-like compounds are identified as useful in the treatment of overweight and obese conditions and disorders and conditions associated with adipose tissue abnormalities. These compounds can be formulated for oral administration.

Disclosed is a method of identifying genes that are over-expressed in adipose tissue as compared to pre-adipocyte tissue or other tissues comprising performing differential gene expression analysis between the white adipose tissue (WAT) or stromal vascular tissue (SVT) from any two different mice selected from the group consisting of wild-type, HMGI-C −/−, ob/ob, and HMGI-C −/− ob/ob genotype mice. Based on this method a number of nucleotide sequences are identified whose expression is adipocyte specific. The identified nucleotide sequences and their corresponding polypeptides are then used to prevent adipogenesis, to treat diabetes, and to screen for small-molecules that can modulate or prevent adipogenesis and to treat diabetes.

The invention discloses a transcription factor C/EBPZ for regulating adipocyte formation and application thereof, belongs to the field of animal molecular genetics and developmental biology, and discloses application of the transcription factor C/EBPZ for regulating and controlling chicken adipocyte formation. The invention discloses an expression mode of chicken C/EBPZ in various tissues and an expression rule of C/EBPZ in a chicken adipose tissue growth and development process by utilizing an RT-PCR technology. According to the invention, an overexpression vector of the transcription factoris constructed, and the C/EBPZ transcription factor is verified to regulate and control the differentiation and proliferation of chicken preadipocytes; the invention further discloses an action mechanism of the transcription factor C/EBPZ in chicken adipose tissue formation. The invention provides a novel application of a C/EBPZ transcription factor in regulation and control of chicken adipocyte formation, and the C/EBPZ transcription factor has practical value in the fields of chicken genetic breeding and animal nutrition.

The invention provides application of golden thread in preparing medicaments, such as an insulin sensitizer and a PPAR-gamma agonist, in particular relates to application in restraining 3T3-L1 pre-adipocyte cell differentiation, enhancing the utilization ratio of 3T3-L1 pre-adipocyte glucose or reducing blood fat and improving insulin resistance or diabetes medicaments. The invention also provides a medicinal composition for restraining 3T3-L1 pre-adipocyte cell differentiation and enhancing the utilization ratio of glucose. The golden thread and the extract thereof have the advantages of restraining 3T3-L1 pre-adipocyte cell differentiation, improving insulin resistance and treating diabetes and complicating diseases thereof, and achieving remarkable medicament effect which is superior to that of berberine hydrochloride and crude golden thread.

The invention discloses a C/EBPZ gene promoter and an application thereof, and belongs to the field of gene engineering and molecular biology. The C/EBPZ gene promoter is any one of A1-A6 sequences of which the nucleotide sequences are as shown in SEQ ID NO: 1-SEQ ID NO: 6. Six chicken C/EBPZ gene promoters are obtained by using DNA extracted from chicken whole blood as a template and using primers for PCR amplification and restriction enzyme digestion, and tests prove that the six promoters all have promoter activity in chicken preadipocytes and are regulated by a specific transcription factor KLF2, so that the promoter has potential application value in revealing a transcription regulation mechanism of chicken C/EBPZ, breeding low-abdominal-fat broilers, revealing molecular mechanisms of various human diseases and developing drugs for targeting C/EBPZ expression.

The invention provides application of wine-roasted Chinese goldthread in preparing medicaments containing insulin sensitizers and PPAR gamma activators. The wine-roasted Chinese goldthread has the functions of inhibiting the differentiation of 3T3-L1 pre-adipocytes, improving the utilization rate of glucose of the 3T3-L1 pre-adipocytes or reducing blood fat as the insulin sensitizers and the application in improving insulin resistance or treating diabetes mellitus. The invention also provides a medicine composition having the functions of inhibiting the differentiation of 3T3-L1 pre-adipocytes and improving the utilization rate of the glucose of the 3T3-L1 pre-adipocytes. The wine-roasted Chinese goldthread and extracts thereof are the medicaments having the function of inhibiting the differentiation of 3T3-L1 pre-adipocytes, can be used for improving insulin resistance and treating the diabetes mellitus and complications thereof and have a specific effect better than that of berberine hydrochloride and crude Chinese goldthread.

The present invention is directed to constructs, compositions and methods for modulating platelet-type 12 lipoxygenase (12-LO) in adipose tissue in vivo. Specifically, the invention provides constructs encoding expression-inhibiting oligonucleic acids, e.g., antisense and RNA interfering (RNAi) molecules, targeted to a platelet-type 12-LO gene or a transcript thereof, which are capable of reducing or silencing platelet-type 12-LO expression specifically in adipocytes and pre-adipocytes. Vectors of these constructs (including, but not limited to, viral vectors), compositions of them and methods of using same for the treatment and amelioration of conditions associated with excess fat cell mass and obesity are also provided.

Dermatological cosmetic combination treatments with high intensity focused ultrasound, dermal fillers, fat-reducing compounds, cavitation-prone fluids, and/or septa dissection are provided. A HIFU therapy system can include an imaging system, methods adapted to alter placement and position of a line focus band therapy, multiple simultaneous cosmetic ultrasound treatment zones in tissue, and dithering ultrasound beams from a transducer to alter placement and position of multiple cosmetic treatment zones in tissue. The HIFU systems can include a hand wand, removable transducer modules, and a control module. The dermal fillers can include hydroxyapatite. The fat-reducing compounds can include adipocytolytic compounds, pentacyclic triterpenoid compounds, proapoptotic compounds, compounds impairing differentiation of pre-adipocytes, and combinations thereof. The cavitation-prone fluids can include molecules dissolved in fluids, ethanol, glycerin, and ethylene glycol. The septa dissecting can include using a cutting blade and/or cavitation HIFU. The cosmetic treatment system may be used in various cosmetic procedures, such as treating cellulite.

The invention discloses a human KLF7 gene promoter as well as a construction method and application thereof, and belongs to the technical field of gene engineering. The nucleotide sequence of the human KLF7 gene promoter is selected from any one sequence as shown in SEQ ID NO: 1 to SEQ ID NO: 18; According to the invention, DNA extracted from human semen is taken as a template, 18 human KLF7 promoters are obtained by using primers for PCR amplification and restriction enzyme digestion, and test verification shows that the 18 promoters all have promoter activity in chicken preadipocytes, so that the human KLF7 gene promoter has important significance for revealing a regulation mechanism of expression of a plurality of human KLF7 transcription spliceosomes and developing drugs for targeting KLF7 expression, and lays a foundation for researching treatment of a plurality of human diseases regulated by the KLF7 gene.