Method for determining leptin

Abstract

The present invention provides a method for the determination of leptin in a sample, based on the detection of leptin-induced angiopoietin in certain cells or cell lines, such as Swiss 3T3 F442A mouse preadipocytes or human bone marrow stromal cell line hMS2-12 -2 Detection of expression. The detection of leptin is as follows: cells are transfected with a vector containing a reporter gene (such as green fluorescent protein (GFP)) under the control of angiopoietin-2 promoter.

Description

field of invention

[0001] The present invention relates to a leptin activity system, more specifically, the present invention relates to in vitro detection of bioactive substances of leptin or leptinoids, and the detection method is suitable for high-efficiency screening of molecular libraries. Background of the invention

[0002] Obesity, defined as excess body fat relative to a lean body mass, is associated with important psychiatric and medical disorders, including hypertension, hyperlipidemia, and type II or non-insulin-dependent diabetes mellitus (NIDDM). There are 6-10 million NIDDM patients in the United States, 18% of whom are 65 years old (Kamel, HK, eta1, Clin Geriatr. Med., 1999, 15, 265). Among obese patients with NIDDM, about 45% of men and 70% of women. By losing weight, their diabetes symptoms were substantially improved or eliminated (Harris 1991, DiabetesCare, 14, 639).

[0003] Leptin is the product of obesity genes (Zhang, Y., et al.1994, Nature 372, 425...