Methacrylic ester polymer, compounds thereof as well as preparation methods and application of all

A technology of methacrylate and compound, which is applied in the field of preparation method and its application as a gene carrier, can solve problems such as difficulty in determining possible toxicity
CN101659722AInactive Publication Date: 2010-03-03SHANGHAI INST OF APPLIED PHYSICS - CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANGHAI INST OF APPLIED PHYSICS - CHINESE ACAD OF SCI
Publication Date
2010-03-03
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a mercaptyl-terminated (2-dimethylamino ethyl) polymethacrylic ester polymer, a self-assembled and modified nano-gold compound (PDMAEMAGNPs) of the polymer as well as a compound of the polymer and plasmid DNA; and the invention also discloses preparation methods of the polymer and the compounds as well as the application of all in carrier as genes. When the concentration ofthe compounds of PDMAEMAGNPs and GFP DNA (green fluorescent protein expression DNA) is 30Mug / mL, the transfection efficiency to HEK 293T cell is 30 percent, and the survival rate to HEK 293T cell, HeLa cell and 293 cell is more than 80 percent. The PDMAEMAGNPs / CpG ODN (human CpG oligodeoxynucleotide) compound has remarkable effects in gene therapy in vitro and in vivo. The suppression ratio to tumor growth of the PDMAEMAGNPs / p53 DNA (tumor suppressor gene) is 30-37 percent.
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Description

technical field

[0001] The present invention relates to a kind of methacrylate polymer, the nano-gold complex of the polymer and the complex of plasmid DNA thereof, and the present invention also relates to the preparation method of these polymers, the complex and their application as gene carriers . Background technique

[0002] Gold nanoparticles have been studied for 150 years. Gold nanoparticles have the advantages of strong oxidation resistance, good biocompatibility, high density and good photoelectric properties. Bioanalysis methods based on gold nanoparticles have achieved many results. Gold nanoclusters (Au 2 MPCs) interact with biological substances such as nucleic acids, proteins, and cell membranes, and can be applied to various research fields of biomedicine.

[0003] CytImmune Sciences of the United States and OctoPlus of the Netherlands have cooperated to develop colloidal gold nanoparticles as a possible delivery technology for the anti-cancer protein TNF ...

Claims

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