Method for preparing cells carrying exogenous molecules in photoinduced perforating mode, base material for preparing cells and cells

Abstract

The invention discloses a method for preparing cells carrying exogenous molecules in a photoinduced perforating mode, a base material for preparing the cells and the cells, belongs to the field of medical instrument modification and the field of nanometer materials, and particularly relates to a reagent kit for modifying the surface of a cell culture base material (including a cell culture plate and other base materials capable of being used for culturing cells) with gold nano-materials (including gold nano particle aggregation, gold nano rods, gold nano cones and the like). The purpose of carrying out exogenous macromolecular transfer on cells cultured on the reagent kit is achieved through near-infrared band laser assistance. According to the system, gold nano particles are used for absorbing a large amount of laser energy at the near-infrared band so as to improve the membrane permeability of the cells cultured on the system; the activity of the cells can be well maintained by optimizing the laser power and the irradiation time; the reagent kit has the advantages of being simple, direct, fast and efficient, and can be applied to different cell lines for transfer of exogenous macromolecules of different types.

Description

technical field [0001] The invention belongs to the field of medical device modification and nanomaterials, and in particular relates to a method for modifying gold nanomaterials (including gold nanoparticle aggregates, The kit of gold nanorods, gold nanocones, etc.) uses near-infrared band laser assistance to achieve the purpose of carrying exogenous macromolecules to the cultured cells on it. The kit is simple, direct, fast and efficient, and can be applied to different cell lines for the delivery of exogenous macromolecules. Background technique [0002] Gene therapy and drug delivery can be applied in the treatment of many major diseases, including cancer, neurodegenerative diseases, blood diseases and genetic diseases. The key to gene therapy and drug delivery is whether exogenous therapeutic molecules (such as DNA, RNA, proteins, drug molecules, etc.) can be efficiently introduced into target cells. Although viral vectors have high delivery efficiency, their safety l...

AI Technical Summary

Problems solved by technology

However, the delivery efficiency of this method is low, and at the same time, it faces the problem of cytotoxicity, and the loaded macromolecules cannot be completely released by the carrier after entering the cell, which further reduces its final efficiency.

[0003] Photoporation has received a lot of attention in recent years, and its simplest form involves irradiating individual cells with high-intensity femtosecond laser pulses to increase cell membrane permeability, but this form is inefficient

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