Three dimensional cell culture

A cell, three-dimensional technology, applied in the field of three-dimensional cell culture system, can solve the problems of explant necrosis, undisclosed preadipocytes, loss of adipocyte structure and functional characteristics

Inactive Publication Date: 2009-06-03
THE OPEN UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Both approaches have disadvantages: the explants rapidly become necrotic, limiting the time in which analysis can be performed, and the cell lines lose the structural and functional characteristics of in vivo adipocytes
[0011] Kuberka et al., Int J Artificial Organs, Vol. 25, 2002, pp. 67-73 describe the creation of a collagen sponge similar to that used by von Heimburg, but do not describe the cultivation of preadipocytes in the sponge
[0012] Therefore, none of these documents discloses the cultivation of isolated preadipocytes in a three-dimensional support matrix and their subsequent differentiation into adipocytes in vitro

Method used

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  • Three dimensional cell culture

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Embodiment Construction

[0034] developed a three-dimensional tissue culture system in which preadipocytes isolated from rats were able to grow and differentiate. Mature adipocytes produced in this manner exhibit morphological and biochemical characteristics similar to those exhibited by adipocytes in vivo and ex vivo, and also by adipocytes grown in two-dimensional culture. behaved differently. Adipocytes cultured in our 3D system maintain viability for several weeks, allowing the study of short-term and long-term function.

[0035] This system provides a more relevant microenvironment for live animals than traditional two-dimensional culture systems.

[0036] It has several areas of application including but not limited to:

[0037] 1. It can be used to culture fat cells alone and study their response to environmental stimuli, including bioactive molecules and therapeutic drugs;

[0038] 2. It can be used to study the interaction between adipocytes and other cell types;

[0039] 3. It can be use...

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Abstract

The present invention describes method for culturing preadipocytes isolated ex vivo, the method including introducing preadipocytes into a three dimensional support matrix, and allowing the cells to differentiate in vitro into adipocytes within the support matrix. The matrix may be a collagen matrix. The method may be used for investigating the development of stem cells, or for investigating the response of adipocytes to stimuli. The method provides a system whereby adipocytes with biological properties resembling those in vivo can be grown in vitro.

Description

field of invention [0001] The present invention relates to a three-dimensional cell culture system for culturing adipocytes. The invention also relates to methods for culturing adipocytes and for obtaining adipocytes from preadipocytes in cell culture. Aspects of the invention also relate to methods for assessing the interaction between adipocytes and other cell types, and methods for assessing the response of adipocytes to environmental stimuli. This enables a better understanding of the mechanisms involved in cell and tissue interactions and has implications in the fields of obesity, diabetes, inflammation and related diseases. Background of the invention [0002] Traditionally, adipose tissue has been primarily considered to be an otherwise inert storage substance. Its functional importance has only attracted attention in recent years. Adipocytes are known to be involved in a range of interactions with other cell types, as well as the production and secretion of differ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/06C12N5/077
CPCC12N2501/33C12N2501/01C12N2533/54C12N5/0653C12N2501/39C12N5/0062
Inventor 希拉里·安妮·麦奎因艾莉森·简·洛克林桑迪普·达亚
Owner THE OPEN UNIV
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