28 results about "Collagen substrate" patented technology

The invention discloses a method for widening keratinized gingiva by using a plasma matrix membrane and a preparation method of the plasma matrix membrane, and the method comprises the following steps: determining an expected widening position of the keratinized gingiva based on preoperative CBCT and intraoral scanning data of a patient; root-direction valve reduction is carried out in the area to be widened, and local original keratinized gingiva with the width of 1-2 mm is reserved; preparing a solid plasma matrix and a liquid plasma matrix by adopting a horizontal centrifugal mode, and preparing the plasma matrix membrane by utilizing a plasma matrix pressurizer; and sewing and fixing the obtained plasma matrix membrane in the to-be-widened area. According to the novel operation mode for widening the keratinized gingiva through the plasma matrix, it can be avoided that a second operation area is opened up to obtain an autograft, the postoperative reaction of a patient is reduced, and the economic burden of the patient is relieved; compared with a finished collagen matrix material, the plasma matrix contains autologous bioactive cells and factors and has a better regeneration promoting function.

The invention discloses a preparation method of an acellular matrix containing a bioactive coating, and belongs to the field of medical beauty industry, the key point of the technical scheme is that the preparation method comprises the preparation steps of S1, preparation of the acellular matrix; s2, treating the acellular matrix; s3, rehydrating the decellularized matrix, sequentially immersing the rehydrated decellularized matrix into an activating solution, a collagen solution and an activating solution, reacting, washing the collagen matrix, and freeze-drying to obtain the collagen matrix; s4, adding the anti-inflammatory microcapsules and the growth factor nano-carrier into the hyaluronic acid solution to obtain a mixed solution; s5, the collagen matrix is immersed in an activating solution, after reaction and washing, the surface of the collagen matrix is coated with the mixed solution for reaction, after curing, washing and drying are conducted, and the acellular matrix containing the bioactive coating is obtained.The covalently-connected hyaluronic acid-collagen double-layer structure is constructed on the surface of the acellular matrix, so that biocompatibility and safety are improved; meanwhile, the problems that a traditional acellular matrix is unstable in structure and poor in mechanical strength are solved.

Improved allografts having controlled and consistent properties and useful for soft tissue repair, including breast reconstruction, and other surgical procedures, are disclosed. In one embodiment, an improved acellular dermal matrix (ADM) is produced from a skin sample, where the ADM consists essentially of a single dermal tissue type (e.g., papillary or reticular) and comprises a collagen matrix having substantially uniform density and porosity. In another embodiment, two ADMs are produced from the same dermal tissue type (e.g., papillary or reticular) of the same skin sample, each of which consists essentially of reticular dermis, and comprises a collagen matrix having substantially uniform density and porosity. All such improved ADMs are suitable for use in breast reconstruction and other tissue repair and modification surgery procedures.

The invention discloses an application of senkyunolide I in preparation of a medicine for treating hepatic fibrosis, which can obviously inhibit HSC (hepatic stellate cell) activation in vitro and evaluate the treatment effect of senkyunolide I on hepatic fibrosis in vivo by adopting a C57BL / 6 mouse hepatic fibrosis model induced by CCl4. The expression levels of liver alpha-smooth muscle actin, type I collagen and matrix metalloproteinase tissue inhibition factor 1 of individuals needing anti-hepatic fibrosis are obviously reduced in vivo and in vitro, and the hepatic fibrosis progress can be effectively intervened. Therefore, according to the application of the senkyunolide I in the hepatic fibrosis, the provided compound senkyunolide I has huge potential for inhibiting the hepatic fibrosis, an effective traditional Chinese medicine monomer component medicine is provided for treating the hepatic fibrosis, the new clinical adaptation application of the senkyunolide I is expanded, and the application prospect is good.

The invention relates to the field of biological medicine, and discloses a nano antibody aiming at DDR1, and a sequence and application thereof. The system disclosed by the invention reveals a DDR1-mediated immune escape network, and completes complete development work from a single-target antibody to a multifunctional fusion protein. The research and development of the DDR1nb-Fc and the derivative fusion protein DDR1nb-Fc-mCD80 of the DDR1nb-Fc and the derivative fusion protein DDR1nb-Fc-mCD80 of the DDR1nb-Fc provide a novel treatment strategy with high efficiency and safety for intractable tumors with abundant collagen matrixes.

Disclosed are implantable devices, systems, and methods of fabricating, testing, optimizing, and / or implanting such devices. An implantable device includes a body, a first intrinsic channel network formed in the body and configured to receive cells, and a second intrinsic channel network formed in the body and configured to allow for active flow of a fluid to support viability and / or function of the cells in the first intrinsic channel network. The first intrinsic channel network comprises a first channel and the second intrinsic channel network comprises a second channel, with at least segments of the first and second channels parallel to and spaced apart from each other. Cells may be suspended in a collagen matrix and introduced to the first intrinsic channel network.

A device may include a decellularized biological scaffold, a first electrode, and a second electrode, wherein the decellularized biological scaffold is in electrical and / or chemical communication with the first and second electrodes. In one example, the device is a battery and the device may include an electrolyte layer supported on the decellularized biological scaffold; an anode layer disposed on a first side of the electrolyte layer; and a cathode layer disposed on second side of the electrolyte layer, opposite the anode layer. The electrolyte layer may include a plant-based conductive hydrogel and / or a PEDOT collagen matrix. The anode and / or the cathode layer may comprise metallic vesicles secreted by a plant.

The present invention discloses a collagen matrix flap covalently bound to fibroblast growth factor and a preparation method thereof. The collagen matrix flap is prepared by cross-linking oxidized sodium alginate (OSA) and bovine type I collagen. Subsequently, polyamide-amine dendrimer (PAMAM) is used as a connection carrier to covalently graft basic fibroblast growth factor (bFGF), thereby constructing a scaffold system capable of long-term sustained release of growth factors. The scaffold has good biocompatibility. Experiments have shown that the scaffold of the present invention exhibits clinical operability, superior biological activity to other growth factor-loaded scaffolds, and a more sustained growth factor release effect.

The invention relates to the technical field of nano biological medicines, and discloses a photo-thermal-enzyme catalysis nano-particle as well as a preparation method and application thereof. The photo-thermal-enzyme catalysis nano-particles are constructed by crosslinking water-soluble self-doped polyaniline nano-particles, human serum albumin, lactate oxidase and collagenase through a thioacetal linker capable of being split by active oxygen. Collagenase and lactate oxidase (LOx) are integrated into the same nanoparticle system through a thioacetal (TK) linker, the TK linker is split under the action of tumor microenvironment active oxygen (ROS) and H2O2 generated by catalysis of the LOx, the activity of the two enzymes is synchronously activated, a collagen matrix (physical barrier) is degraded, lactic acid (chemical barrier) is removed, the tumor microenvironment is efficiently remodeled, and the tumor microenvironment can be effectively inhibited. The problems that the regulation effect of a single barrier is limited, and the tumor microenvironment is difficult to improve are solved.

Described is a method of repairing the parenchyma of the brain. The method comprises implanting a biodegradable porous polymeric matrix block, such as a piece of collagen matrix, to a void in the brain through a tubular device. A medical device for use in the method is also described.

Disclosed are implantable devices, systems, and methods of fabricating, testing, optimizing, and / or implanting such devices. An implantable device includes a body, a first intrinsic channel network formed in the body and configured to receive cells, and a second intrinsic channel network formed in the body and configured to allow for active flow of a fluid to support viability and / or function of the cells in the first intrinsic channel network. The first intrinsic channel network comprises a first channel and the second intrinsic channel network comprises a second channel, with at least segments of the first and second channels parallel to and spaced apart from each other. Cells may be suspended in a collagen matrix and introduced to the first intrinsic channel network.

Hydrogels comprising a macromolecular matrix and water may be used for aesthetic fillers, for example, dermal fillers. The macromolecular matrix may include a crosslinked combination of hyaluronic acid and collagen.

The invention provides an application of polyclonal Treg cells in treatment of maxillary hypoplasia, and relates to the technical field of biological medicines.The experiment proves that the polyclonal Treg cells can promote the increase of the volume of the middle seam of the maxillary palate; the generation of soft tissues in the middle seam of the maxillary palate can be effectively promoted; the synthesis of a collagen matrix in a palate center seam area can be promoted; the polyclonal Treg cell can be used as an immune cell to be applied to a medicine for treating maxillary dysplasia.

The invention relates to a bionic collagen matrix with a density gradient double-layer structure, and belongs to the technical field of medical materials. The collagen matrix takes telopeptide collagen derived from mammalian dermis or achilles tendon as a main component, and comprises a double-layer structure of a compact layer and a loose layer, and the compact layer is smooth in surface and compact in structure, so that cell migration can be blocked, and a certain protection effect is achieved; the loose layer is of a porous structure, cell ingrowth and blood clot stabilization are facilitated, and a support is provided for tissue repair. The preparation method of the biological membrane comprises the following steps: preparing telopeptide-removed collagen gel, preparing collagen sponge, preparing a collagen tissue regeneration membrane, and compounding the collagen tissue regeneration membrane and the collagen sponge into an integrated double-layer structure through bonding, drying and crosslinking. The double-layer structure simultaneously meets the double requirements of barrier protection and tissue regeneration, the process is simple and controllable, and the double-layer structure is suitable for clinical scenes such as oral soft tissue repair, alveolar ridge reconstruction and tooth extraction pit coverage.

Disclosed are implantable devices, systems, and methods of fabricating, testing, optimizing, and / or implanting such devices. An implantable device includes a body, a first intrinsic channel network formed in the body and configured to receive cells, and a second intrinsic channel network formed in the body and configured to allow for active flow of a fluid to support viability and / or function of the cells in the first intrinsic channel network. The first intrinsic channel network comprises a first channel and the second intrinsic channel network comprises a second channel, with at least segments of the first and second channels parallel to and spaced apart from each other. Cells may be suspended in a collagen matrix and introduced to the first intrinsic channel network.

The application discloses a preparation method of a collagen matrix multifunctional self-power generation heart valve repair material, and specifically comprises the following steps: firstly, stirring and mixing an ammonium sulfate solution, gelatin, acrylic acid and acrylamide, then stirring and adding N, N-methylene bisacrylamide, and then adopting electrostatic spinning to form a film to obtain a power generation layer; secondly, preparing a bonding layer by using sodium carboxymethyl cellulose and a pyrrole monomer; and finally, using a decellularized pig dermal matrix as a support, sequentially bonding the power generation layer and the bonding layer with a chitosan solution, and the heart valve repair material is prepared. The heart valve repair material prepared by the application has the function of self-power generation, can self-generate a micro-current to implement electric stimulation on a heart valve wound site, further promotes wound healing, and can also reduce the risk of infection and pain of a patient.

Disclosed are implantable devices, systems, and methods of fabricating, testing, optimizing, and / or implanting such devices. An implantable device includes a body, a first intrinsic channel network formed in the body and configured to receive cells, and a second intrinsic channel network formed in the body and configured to allow for active flow of a fluid to support viability and / or function of the cells in the first intrinsic channel network. The first intrinsic channel network comprises a first channel and the second intrinsic channel network comprises a second channel, with at least segments of the first and second channels parallel to and spaced apart from each other. Cells may be suspended in a collagen matrix and introduced to the first intrinsic channel network.

The invention relates to the field of biological medicines, and discloses a nano antibody fusion protein aiming at DDR1, and a sequence and application thereof. The system disclosed by the invention reveals a DDR1-mediated immune escape network, and completes complete development work from a single-target antibody to a multifunctional fusion protein. The research and development of the DDR1nb-Fc and the derivative fusion protein DDR1nb-Fc-mCD80 of the DDR1nb-Fc and the derivative fusion protein DDR1nb-Fc-mCD80 of the DDR1nb-Fc provide a novel treatment strategy with high efficiency and safety for intractable tumors with abundant collagen matrixes.

Disclosed is a preparation method of a collagen matrix membrane and an application thereof. The invention uses animal intestinal casing as a raw material and sequentially includes steps such as decellularization and degreasing to prepare a collagen matrix membrane with excellent mechanical properties and a long degradation cycle. This membrane is more conducive to providing sufficient bone regeneration space and time during the initial healing phase of periodontal disease, better meeting clinical application needs. Additionally, the collagen matrix membrane prepared by the invention is loaded with multiple growth factors beneficial to periodontal tissue growth, thereby facilitating periodontal tissue regeneration and repair, and improving therapeutic efficacy.

Improved allografts having controlled and consistent properties and useful for soft tissue repair, including breast reconstruction, and other surgical procedures, are disclosed. In one embodiment, an improved acellular dermal matrix (ADM) is produced from a skin sample, where the ADM consists essentially of a single dermal tissue type (e.g., papillary or reticular) and comprises a collagen matrix having substantially uniform density and porosity. In another embodiment, two ADMs are produced from the same dermal tissue type (e.g., papillary or reticular) of the same skin sample, each of which consists essentially of reticular dermis, and comprises a collagen matrix having substantially uniform density and porosity. All such improved ADMs are suitable for use in breast reconstruction and other tissue repair and modification surgery procedures.

This invention discloses a method for preparing a decellularized matrix with a bioactive coating, belonging to the medical aesthetics industry. The key technical points of the method are the following steps: S1, preparation of the decellularized matrix; S2, treatment of the decellularized matrix; S3, rehydrating the decellularized matrix and then sequentially immersing it in an activation solution, a collagen solution, and another activation solution, followed by washing and freeze-drying to obtain a collagen matrix; S4, adding anti-inflammatory microcapsules and growth factor nanocarriers to a hyaluronic acid solution to obtain a mixed solution; S5, immersing the collagen matrix in the activation solution, reacting and rinsing, coating the mixed solution onto the surface of the collagen matrix, then curing, rinsing, and drying to obtain a decellularized matrix with a bioactive coating. This application improves biocompatibility and safety by constructing a covalently linked hyaluronic acid-collagen bilayer structure on the surface of the decellularized matrix, while also solving the problems of unstable structure and poor mechanical strength of traditional decellularized matrices.

The invention discloses a preparation method of a bio-based film based on natural collagen. The preparation method specifically comprises the following steps: preparing a collagen matrix material; mixing grease and a surfactant to obtain a softening agent; mixing water and polyhydric alcohol to obtain filling liquid; mixing a collagen matrix material, water and the bleaching liquid, adding the mixture into a rotary drum, and rotating; adding the treated collagen matrix material, water and a softening agent into a rotary drum to rotate; and adding the treated collagen matrix material and the filling liquid into a rotary drum, rotating, adjusting the pH value, and drying the collagen matrix. The bio-based film provided by the invention takes natural animal skin as a base material, and the natural animal skin becomes an ideal carrier for loading various materials due to the inherent tissue network and hierarchical structure of the bio-based film. And the collagenous fibers serve as a frame for bearing and dissipating energy, external mechanical energy is effectively distributed through sliding and deformation in the layered structure of the fiber network, and high mechanical strength is achieved. And meanwhile, through filling of the binary solvent and lubrication of the softening agent, the bio-based material is endowed with relatively high toughness.

A bone implant body serves to be introduced into a bone defect location to promote bone growth in this bone defect location. It has a main body made of a deformable and porous collagen matrix, which contains at least one main body collagen material of a first proportion in the form of a mineralized collagen and a second proportion in the form of a native collagen, and a depot member, which is completely embedded in the main body such that the depot member is completely surrounded by the collagen matrix of the main body and placed centrally within the main body and contains, as a first constituent, at least one depot material in the form of at least one bioactive substance which promotes bone healing. A transition between the collagen matrix of the main body and the depot member is formed by an interfacial area of the depot member.

The invention provides an overdyeing kit capable of simultaneously detecting CD8 + T cells and collagen and application of the overdyeing kit, and belongs to the technical field of pathological detection. The overdyeing kit capable of simultaneously detecting the CD8 + T cells and the collagen, provided by the invention, comprises a CD8 immunohistochemical staining reagent and an improved aniline blue staining kit. According to the overdyeing kit provided by the invention, the spatial distribution of the collagen matrix and the CD8 + T cells in the tumor microenvironment can be synchronously detected by a single slice through an overdyeing strategy, the CD8 + T cells (brown) and collagenous fibers (blue-green) can be respectively and specifically marked, the dyeing contrast ratio is remarkably improved, and meanwhile, the histomorphological integrity is reserved. Compared with the traditional step-by-step staining method, the overdyeing kit provided by the invention breaks through the technical bottlenecks of high consumption of tissue samples and complex operation in multiple staining.

The invention relates to the technical field of biomedical materials, discloses a preparation method of a bone repair mineralized biological membrane capable of inducing gene expression and a product, and aims at solving the problems that an existing bone repair material HAP crystal is difficult to regulate and control, the mechanical activity and the biological activity are not matched, bone induction is weak, the process is complex and the like. The preparation method comprises the following steps: S1, preparing a decellularized small intestinal submucosa (SIS) membrane and sterilizing; s2, constructing a pDA-PAM (at) SIS composite membrane; s3, inducing directional growth of HAP through ion permeation biomimetic mineralization; and S4, cleaning and drying to obtain the HAP / SIS collagen mineralized biological membrane. The product contains an SIS collagen matrix and low-crystallinity nano HAP (the width is 4-20nm, the length is 31-105nm, and the product is oriented along a c axis), the tensile strength is greater than or equal to 13MPa, the elastic modulus is greater than or equal to 75MPa, the specific surface area is greater than or equal to 60m < 2 > / g, and osteogenic genes such as ALP, Runx2 and the like can be up-regulated by more than or equal to 2 times. The method is mild in process, easy to operate, good in biocompatibility, high in bone conduction and inductivity, suitable for large-scale production and capable of meeting the clinical bone defect repair requirement.

The invention discloses a histamine modified collagen matrix material as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The histamine modified collagen matrix material comprises rat tail type 1 collagen, calcium chloride, histamine hydrochloride, recombinant guinea pig liver TGM2, Tris alkali, a PBS buffer solution and dd water. The final concentration of each component is as follows: the rat tail type 1 collagen solution is 1-3 mg / mL; 4-6 mM of a calcium chloride solution; 10-30 [mu] g / mL of a histamine hydrochloride solution; 0.1-0.3 U / mL of a recombinant guinea pig liver TGM2 solution; and neutralizing with Tris alkali until the pH value is 7.2-7.4. Based on two different physiological effects of mediating collagen crosslinking and mediating histamine modification of TGM2, the histamine modified collagen matrix is constructed, and compared with a common collagen matrix, the histamine modified collagen matrix has superior mechanical properties and can be used as a medical material for drug delivery, coating and dressing.

The application particularly relates to an artificial skin with antibacterial and inflammation regulating functions and a preparation method thereof, and belongs to the technical field of artificial skin. The artificial skin is obtained by combining a dermis layer formed by doping silver sulfadiazine and loading simvastatin polylactic acid microspheres in collagen as a carrier material with a silicone epidermis layer. The silver sulfadiazine loaded in the collagen matrix can effectively avoid bacterial infection, the slow-release function of the polylactic acid microspheres can release simvastatin in the later stage of skin regeneration, promote the conversion of macrophages to M2 type, participate in the anti-inflammatory and repair processes, and accelerate skin regeneration. The silicone epidermis layer can prevent the loss of body fluid and bacterial invasion.