42 results about "Collagen substrate" patented technology

The invention discloses a method for widening keratinized gingiva by using a plasma matrix membrane and a preparation method of the plasma matrix membrane, and the method comprises the following steps: determining an expected widening position of the keratinized gingiva based on preoperative CBCT and intraoral scanning data of a patient; root-direction valve reduction is carried out in the area to be widened, and local original keratinized gingiva with the width of 1-2 mm is reserved; preparing a solid plasma matrix and a liquid plasma matrix by adopting a horizontal centrifugal mode, and preparing the plasma matrix membrane by utilizing a plasma matrix pressurizer; and sewing and fixing the obtained plasma matrix membrane in the to-be-widened area. According to the novel operation mode for widening the keratinized gingiva through the plasma matrix, it can be avoided that a second operation area is opened up to obtain an autograft, the postoperative reaction of a patient is reduced, and the economic burden of the patient is relieved; compared with a finished collagen matrix material, the plasma matrix contains autologous bioactive cells and factors and has a better regeneration promoting function.

The invention discloses a preparation method of an acellular matrix containing a bioactive coating, and belongs to the field of medical beauty industry, the key point of the technical scheme is that the preparation method comprises the preparation steps of S1, preparation of the acellular matrix; s2, treating the acellular matrix; s3, rehydrating the decellularized matrix, sequentially immersing the rehydrated decellularized matrix into an activating solution, a collagen solution and an activating solution, reacting, washing the collagen matrix, and freeze-drying to obtain the collagen matrix; s4, adding the anti-inflammatory microcapsules and the growth factor nano-carrier into the hyaluronic acid solution to obtain a mixed solution; s5, the collagen matrix is immersed in an activating solution, after reaction and washing, the surface of the collagen matrix is coated with the mixed solution for reaction, after curing, washing and drying are conducted, and the acellular matrix containing the bioactive coating is obtained.The covalently-connected hyaluronic acid-collagen double-layer structure is constructed on the surface of the acellular matrix, so that biocompatibility and safety are improved; meanwhile, the problems that a traditional acellular matrix is unstable in structure and poor in mechanical strength are solved.

The invention discloses a collagen-based and high-transparency matrix for cornea thickening and a preparation method of the collagen-based and high-transparency matrix. The preparation method comprises the following steps: assembling a collagen composite solution into a collagen matrix implant on a set curvature mold through electrochemical deposition; the collagen composite solution is prepared from collagen, glacial acetic acid, hydrogen peroxide, heparin and water; putting the obtained collagen matrix implant and a mold into a cross-linking agent solution for cross-linking; cleaning the residual cross-linking agent after completion, separating the collagen matrix implant from the mold, and placing the collagen matrix implant under an ultraviolet light source for cross-linking to obtain a double-cross-linked collagen matrix implant; and soaking the double-crosslinked collagen matrix implant in a nerve growth factor solution, and performing absorption and combination to obtain the collagen-based and high-transparency matrix for cornea thickening. According to the invention, an electro-deposition assembly technical scheme is adopted, the diameter and thickness of collagen assembly fibers are adjusted and controlled through electric field energy, and the collagen assembly fibers are orderly and regularly arranged, so that scattering and refraction of light are reduced, the light transmittance of cornea is increased and can reach more than 90%, and the problem of light blocking caused by materials is effectively avoided. Operation is matched with femtosecond equipment, operation is easy, consumed time is short, and trauma is small.

Improved allografts having controlled and consistent properties and useful for soft tissue repair, including breast reconstruction, and other surgical procedures, are disclosed. In one embodiment, an improved acellular dermal matrix (ADM) is produced from a skin sample, where the ADM consists essentially of a single dermal tissue type (e.g., papillary or reticular) and comprises a collagen matrix having substantially uniform density and porosity. In another embodiment, two ADMs are produced from the same dermal tissue type (e.g., papillary or reticular) of the same skin sample, each of which consists essentially of reticular dermis, and comprises a collagen matrix having substantially uniform density and porosity. All such improved ADMs are suitable for use in breast reconstruction and other tissue repair and modification surgery procedures.

The invention discloses an application of senkyunolide I in preparation of a medicine for treating hepatic fibrosis, which can obviously inhibit HSC (hepatic stellate cell) activation in vitro and evaluate the treatment effect of senkyunolide I on hepatic fibrosis in vivo by adopting a C57BL / 6 mouse hepatic fibrosis model induced by CCl4. The expression levels of liver alpha-smooth muscle actin, type I collagen and matrix metalloproteinase tissue inhibition factor 1 of individuals needing anti-hepatic fibrosis are obviously reduced in vivo and in vitro, and the hepatic fibrosis progress can be effectively intervened. Therefore, according to the application of the senkyunolide I in the hepatic fibrosis, the provided compound senkyunolide I has huge potential for inhibiting the hepatic fibrosis, an effective traditional Chinese medicine monomer component medicine is provided for treating the hepatic fibrosis, the new clinical adaptation application of the senkyunolide I is expanded, and the application prospect is good.

The invention relates to the field of biological medicine, and discloses a nano antibody aiming at DDR1, and a sequence and application thereof. The system disclosed by the invention reveals a DDR1-mediated immune escape network, and completes complete development work from a single-target antibody to a multifunctional fusion protein. The research and development of the DDR1nb-Fc and the derivative fusion protein DDR1nb-Fc-mCD80 of the DDR1nb-Fc and the derivative fusion protein DDR1nb-Fc-mCD80 of the DDR1nb-Fc provide a novel treatment strategy with high efficiency and safety for intractable tumors with abundant collagen matrixes.

Disclosed are implantable devices, systems, and methods of fabricating, testing, optimizing, and / or implanting such devices. An implantable device includes a body, a first intrinsic channel network formed in the body and configured to receive cells, and a second intrinsic channel network formed in the body and configured to allow for active flow of a fluid to support viability and / or function of the cells in the first intrinsic channel network. The first intrinsic channel network comprises a first channel and the second intrinsic channel network comprises a second channel, with at least segments of the first and second channels parallel to and spaced apart from each other. Cells may be suspended in a collagen matrix and introduced to the first intrinsic channel network.

A device may include a decellularized biological scaffold, a first electrode, and a second electrode, wherein the decellularized biological scaffold is in electrical and / or chemical communication with the first and second electrodes. In one example, the device is a battery and the device may include an electrolyte layer supported on the decellularized biological scaffold; an anode layer disposed on a first side of the electrolyte layer; and a cathode layer disposed on second side of the electrolyte layer, opposite the anode layer. The electrolyte layer may include a plant-based conductive hydrogel and / or a PEDOT collagen matrix. The anode and / or the cathode layer may comprise metallic vesicles secreted by a plant.

The present invention discloses a collagen matrix flap covalently bound to fibroblast growth factor and a preparation method thereof. The collagen matrix flap is prepared by cross-linking oxidized sodium alginate (OSA) and bovine type I collagen. Subsequently, polyamide-amine dendrimer (PAMAM) is used as a connection carrier to covalently graft basic fibroblast growth factor (bFGF), thereby constructing a scaffold system capable of long-term sustained release of growth factors. The scaffold has good biocompatibility. Experiments have shown that the scaffold of the present invention exhibits clinical operability, superior biological activity to other growth factor-loaded scaffolds, and a more sustained growth factor release effect.

The invention relates to the technical field of nano biological medicines, and discloses a photo-thermal-enzyme catalysis nano-particle as well as a preparation method and application thereof. The photo-thermal-enzyme catalysis nano-particles are constructed by crosslinking water-soluble self-doped polyaniline nano-particles, human serum albumin, lactate oxidase and collagenase through a thioacetal linker capable of being split by active oxygen. Collagenase and lactate oxidase (LOx) are integrated into the same nanoparticle system through a thioacetal (TK) linker, the TK linker is split under the action of tumor microenvironment active oxygen (ROS) and H2O2 generated by catalysis of the LOx, the activity of the two enzymes is synchronously activated, a collagen matrix (physical barrier) is degraded, lactic acid (chemical barrier) is removed, the tumor microenvironment is efficiently remodeled, and the tumor microenvironment can be effectively inhibited. The problems that the regulation effect of a single barrier is limited, and the tumor microenvironment is difficult to improve are solved.

Described is a method of repairing the parenchyma of the brain. The method comprises implanting a biodegradable porous polymeric matrix block, such as a piece of collagen matrix, to a void in the brain through a tubular device. A medical device for use in the method is also described.

The food additive lauryl gallate (LG, E312) is a molecule with antioxidant and hydrophobic properties which has also been shown to exert antibacterial, antiviral and anti-tumoral effects. Here, the inventors show that LG at a low concentration has the ability to spontaneously induce washed human platelet shape change, filopodia emission, granule secretion, phosphatidylserine expression and aggregation. LG was able to activate intracellular signaling pathways including Akt, p38MAP-kinase and calcium response and to trigger activation of the αIIbβ3 integrin. LG also significantly potentiated platelet aggregation induced by low doses of collagen or thrombin receptor agonist peptide. Consistent with this, low doses of LG added to human blood promoted a strong platelet thrombotic response under arterial flow on a collagen matrix. As shown by electron microscopy, at a high concentration, LG induced a dramatic platelet membrane modification associated with calcium influx and a slow platelet aggregation response. Finally, a local flash-application of LG efficiently decreased the tail bleeding in rats suggesting that this compound has the potential to act as a hemostatic. Overall, the results indicate that the food additive LG, possibly through its capacity to modify membrane lateral organization, has pro-aggregant and antihemorrhagic properties.

Disclosed are implantable devices, systems, and methods of fabricating, testing, optimizing, and / or implanting such devices. An implantable device includes a body, a first intrinsic channel network formed in the body and configured to receive cells, and a second intrinsic channel network formed in the body and configured to allow for active flow of a fluid to support viability and / or function of the cells in the first intrinsic channel network. The first intrinsic channel network comprises a first channel and the second intrinsic channel network comprises a second channel, with at least segments of the first and second channels parallel to and spaced apart from each other. Cells may be suspended in a collagen matrix and introduced to the first intrinsic channel network.

The invention discloses a sampling detection device for collagen matrix preparation, and relates to the field of collagen matrix sampling, the sampling detection device comprises a base, a rotating seat, a mechanical arm and an air cylinder, the rotating seat driven by a motor is installed on the base, the mechanical arm is fixed on the rotating seat, the air cylinder is fixed on the mechanical arm, and the air cylinder is fixed on the rotating seat. A limiting mechanism is fixed to the output end of the air cylinder, a sampling mechanism is fixed to the lower end of the limiting mechanism, and a scraping mechanism is arranged on the outer side of the sampling mechanism. According to the sampling detection device for collagen matrix preparation, after sampling is completed and a sampling mechanism is separated from the liquid level of a collagen matrix, a convex shaft is driven to move through contraction of an air cylinder, and a scraping plate rotates around the outer wall of a sampling barrel in cooperation with the sliding effect between the convex shaft and a sliding groove spiral structure, so that the effect of scraping the collagen matrix on the outer wall of the sampling barrel is achieved; waste of the collagen matrix adhered to the outer wall of the sampling barrel is avoided, manual treatment is not needed in the whole operation, and the use convenience of the device is improved.

Hydrogels comprising a macromolecular matrix and water may be used for aesthetic fillers, for example, dermal fillers. The macromolecular matrix may include a crosslinked combination of hyaluronic acid and collagen.

The invention provides an application of polyclonal Treg cells in treatment of maxillary hypoplasia, and relates to the technical field of biological medicines.The experiment proves that the polyclonal Treg cells can promote the increase of the volume of the middle seam of the maxillary palate; the generation of soft tissues in the middle seam of the maxillary palate can be effectively promoted; the synthesis of a collagen matrix in a palate center seam area can be promoted; the polyclonal Treg cell can be used as an immune cell to be applied to a medicine for treating maxillary dysplasia.

The invention relates to a bionic collagen matrix with a density gradient double-layer structure, and belongs to the technical field of medical materials. The collagen matrix takes telopeptide collagen derived from mammalian dermis or achilles tendon as a main component, and comprises a double-layer structure of a compact layer and a loose layer, and the compact layer is smooth in surface and compact in structure, so that cell migration can be blocked, and a certain protection effect is achieved; the loose layer is of a porous structure, cell ingrowth and blood clot stabilization are facilitated, and a support is provided for tissue repair. The preparation method of the biological membrane comprises the following steps: preparing telopeptide-removed collagen gel, preparing collagen sponge, preparing a collagen tissue regeneration membrane, and compounding the collagen tissue regeneration membrane and the collagen sponge into an integrated double-layer structure through bonding, drying and crosslinking. The double-layer structure simultaneously meets the double requirements of barrier protection and tissue regeneration, the process is simple and controllable, and the double-layer structure is suitable for clinical scenes such as oral soft tissue repair, alveolar ridge reconstruction and tooth extraction pit coverage.

The invention discloses an efficient synthesis process of aquatic collagen tripeptide, and belongs to the technical field of collagen tripeptide production. The method is used for solving the technical problems that in the prior art, when collagen tripeptide is produced through a collagen substrate enzymolysis technology, multiple times of filtration and purification are needed, the operation process is complex, and the production cost is high. A high-efficiency synthesis process of aquatic collagen tripeptide comprises the following steps: S1, blending pretreated fish skin, deionized water and mixed enzyme, preserving heat, performing enzymolysis, performing enzyme deactivation in the deionized water, filtering, and collecting filtrate; s2, filtering the filtrate by using a composite reverse osmosis membrane to obtain concentrated collagen tripeptide; and carrying out spray drying on the concentrated collagen tripeptide to finally prepare the collagen tripeptide. According to the method, fish skin is used as a substrate, and the aquatic collagen tripeptide is prepared through primary enzymolysis and primary reverse osmosis membrane filtration. The collagen tripeptide prepared by the invention has the advantages of high product purity and simplified process.

Disclosed are implantable devices, systems, and methods of fabricating, testing, optimizing, and / or implanting such devices. An implantable device includes a body, a first intrinsic channel network formed in the body and configured to receive cells, and a second intrinsic channel network formed in the body and configured to allow for active flow of a fluid to support viability and / or function of the cells in the first intrinsic channel network. The first intrinsic channel network comprises a first channel and the second intrinsic channel network comprises a second channel, with at least segments of the first and second channels parallel to and spaced apart from each other. Cells may be suspended in a collagen matrix and introduced to the first intrinsic channel network.

Radioactive seeds may be attached to wrappers made of various bio-compatible materials such as plastics, polymers, or shielding materials. These wrappers may be inserted (at least partially) into a carrier (e.g., a collagen substrate) to position radioactive seeds at desired locations within the carrier. Wrappers may each have a single layer or multiple discrete layers, and some may include shielding materials. A wrapper may shield radiation in certain directions while allowing it to emit into the patient tissue for therapeutic purposes. The size of the wrapper opening can be customized for specific patients or treatment plans, enabling controlled radiation delivery areas.

The invention discloses a bioprosthetic valve material of a bionic endothelial glycocalyx-like hydrogel coating and a preparation method of the bioprosthetic valve material, and the preparation method comprises the following steps: S1, obtaining a biological material, and pretreating the biological material; s2, preparing an activated caffeic acid solution, and soaking the pretreated biological material into the activated caffeic acid solution, so that caffeic acid is grafted on the biological material; and S3, preparing an Alg-DA solution containing an initiator, immersing the grafted biological material into the Alg-DA solution, carrying out an oxidative coupling reaction, and after the reaction is finished, carrying out cleaning so as to obtain the bioprosthetic valve material with the bionic endothelial glycocalyx-like hydrogel coating. The biomimetic endothelial glycocalyx-like hydrogel coating can be constructed on the thrombogenic collagen matrix of the bioprosthetic valve to simulate the good antithrombotic performance of normal blood vessels, and the blood compatibility of the bioprosthetic valve is remarkably improved.

The application discloses a preparation method of a collagen matrix multifunctional self-power generation heart valve repair material, and specifically comprises the following steps: firstly, stirring and mixing an ammonium sulfate solution, gelatin, acrylic acid and acrylamide, then stirring and adding N, N-methylene bisacrylamide, and then adopting electrostatic spinning to form a film to obtain a power generation layer; secondly, preparing a bonding layer by using sodium carboxymethyl cellulose and a pyrrole monomer; and finally, using a decellularized pig dermal matrix as a support, sequentially bonding the power generation layer and the bonding layer with a chitosan solution, and the heart valve repair material is prepared. The heart valve repair material prepared by the application has the function of self-power generation, can self-generate a micro-current to implement electric stimulation on a heart valve wound site, further promotes wound healing, and can also reduce the risk of infection and pain of a patient.

Disclosed are implantable devices, systems, and methods of fabricating, testing, optimizing, and / or implanting such devices. An implantable device includes a body, a first intrinsic channel network formed in the body and configured to receive cells, and a second intrinsic channel network formed in the body and configured to allow for active flow of a fluid to support viability and / or function of the cells in the first intrinsic channel network. The first intrinsic channel network comprises a first channel and the second intrinsic channel network comprises a second channel, with at least segments of the first and second channels parallel to and spaced apart from each other. Cells may be suspended in a collagen matrix and introduced to the first intrinsic channel network.

The invention discloses a collagen matrix membrane preparation method and application thereof.The collagen matrix membrane which is excellent in mechanical property and long in degradation period is prepared by taking animal casings as raw materials and sequentially including the steps of membrane laying, gradient freeze-drying and the like, sufficient bone regeneration space and time can be better provided in the early stage of periodontal disease healing, and the collagen matrix membrane can be used for treating periodontal diseases. The clinical application is better met; moreover, the collagen matrix membrane prepared by the invention is loaded with a plurality of growth fiber factors beneficial to the growth of periodontium, so that the regeneration and repair of the periodontium are facilitated, and the treatment effect is improved.

The invention discloses an electrospun collagen complex as well as a preparation method and application thereof, and belongs to the technical field of biological materials. The preparation method disclosed by the invention comprises the following steps: S1, preparing a complex MQA; s2, preparing an electrostatic spinning type I collagen matrix Col; and S3, preparing a suspension from the complex MQA by using a chitosan-acetic acid solution, dipping an electrostatic spinning type I collagen matrix Col in the suspension, carrying out ultrasonic treatment, taking out the electrostatic spinning type I collagen matrix Col, cleaning, and drying to obtain the electrospun collagen complex. The invention also discloses the electrospun collagen complex prepared by the method, and application of the electrospun collagen complex in preparation of medicines for preventing or / and treating decayed teeth. The electrospun collagen complex provided by the invention can realize a caries bionic repair effect integrating enamel biomimetic mineralization, dentin biomimetic mineralization and promotion of dental pulp cells to dentin differentiation.

A method for stabilization of collagen matrices and of condensation of natural and synthetic polymers that uses 2-halo-4,6-dialkoxy-1,3,5-triazines in the presence of one or more amines as activating agents for reactions of crosslinking, condensation, grafting, and curing of collagen matrices, cellulose, modified celluloses, polysaccharides, acid unsaturated polymers, and chiral and non-chiral amines, etc. Forming an integral part of the present invention is also the method for production on an industrial scale of 2-halo-4,6-dialkoxy-1,3,5-triazines. Additionally provided is a pair of reagents for stabilization of collagen matrices and for condensation of polymers.

The invention relates to the field of biological medicines, and discloses a nano antibody fusion protein aiming at DDR1, and a sequence and application thereof. The system disclosed by the invention reveals a DDR1-mediated immune escape network, and completes complete development work from a single-target antibody to a multifunctional fusion protein. The research and development of the DDR1nb-Fc and the derivative fusion protein DDR1nb-Fc-mCD80 of the DDR1nb-Fc and the derivative fusion protein DDR1nb-Fc-mCD80 of the DDR1nb-Fc provide a novel treatment strategy with high efficiency and safety for intractable tumors with abundant collagen matrixes.

Disclosed is a preparation method of a collagen matrix membrane and an application thereof. The invention uses animal intestinal casing as a raw material and sequentially includes steps such as decellularization and degreasing to prepare a collagen matrix membrane with excellent mechanical properties and a long degradation cycle. This membrane is more conducive to providing sufficient bone regeneration space and time during the initial healing phase of periodontal disease, better meeting clinical application needs. Additionally, the collagen matrix membrane prepared by the invention is loaded with multiple growth factors beneficial to periodontal tissue growth, thereby facilitating periodontal tissue regeneration and repair, and improving therapeutic efficacy.

Improved allografts having controlled and consistent properties and useful for soft tissue repair, including breast reconstruction, and other surgical procedures, are disclosed. In one embodiment, an improved acellular dermal matrix (ADM) is produced from a skin sample, where the ADM consists essentially of a single dermal tissue type (e.g., papillary or reticular) and comprises a collagen matrix having substantially uniform density and porosity. In another embodiment, two ADMs are produced from the same dermal tissue type (e.g., papillary or reticular) of the same skin sample, each of which consists essentially of reticular dermis, and comprises a collagen matrix having substantially uniform density and porosity. All such improved ADMs are suitable for use in breast reconstruction and other tissue repair and modification surgery procedures.

This invention discloses a method for preparing a decellularized matrix with a bioactive coating, belonging to the medical aesthetics industry. The key technical points of the method are the following steps: S1, preparation of the decellularized matrix; S2, treatment of the decellularized matrix; S3, rehydrating the decellularized matrix and then sequentially immersing it in an activation solution, a collagen solution, and another activation solution, followed by washing and freeze-drying to obtain a collagen matrix; S4, adding anti-inflammatory microcapsules and growth factor nanocarriers to a hyaluronic acid solution to obtain a mixed solution; S5, immersing the collagen matrix in the activation solution, reacting and rinsing, coating the mixed solution onto the surface of the collagen matrix, then curing, rinsing, and drying to obtain a decellularized matrix with a bioactive coating. This application improves biocompatibility and safety by constructing a covalently linked hyaluronic acid-collagen bilayer structure on the surface of the decellularized matrix, while also solving the problems of unstable structure and poor mechanical strength of traditional decellularized matrices.