53 results about "Collagen substrate" patented technology

A felt for repairing soft tissue defects comprising a membranous collagen substrate and a bioresorbable fiber felted onto the collagen substrate. Methods of preparing a felt and methods of repairing soft tissue damage with a felt are also provided.

The present invention relates to degradation-stabilised, biocompatible collagen matrices which are distinguished in particular by the fact that they contain soluble collagen and peptide constituents, to processes for the preparation of such collagen matrices, which processes include in particular chemical crosslinking with an epoxy-functional crosslinking agent, and to the use of the collagen matrices according to the invention as a cosmetic or pharmaceutical agent, in particular for topical use, and as a wound treatment agent, as an implant or as a haemostatic agent in humans or animals, and as a scaffold for cell population in the biotechnology, basic research and tissue engineering field.

A collagen-binding MSCRAMM entitled Ace from enterococcal bacteria is provided which was homologous to the ligand-binding region of Cna, the collagen-binding MSCRAMM from Staphylococcus aureus, and which can be utilized to inhibit adhesion of enterococcal bacteria to extracellular matrix proteins. The N-terminal region of Ace contained a region (residues 174-319), or A domain, contains several 47-residue tandem repeat units between the collagen-binding site and cell wall-associated regions. The Ace protein can be utilized in methods of preventing and / or treating enterococcal infection, and in addition, antibodies raised against Ace, or its A domain, can be used to effectively inhibit the adhesion of enterococcal cells to a collagen substrate.

The present invention describes method for culturing preadipocytes isolated ex vivo, the method including introducing preadipocytes into a three dimensional support matrix, and allowing the cells to differentiate in vitro into adipocytes within the support matrix. The matrix may be a collagen matrix. The method may be used for investigating the development of stem cells, or for investigating the response of adipocytes to stimuli. The method provides a system whereby adipocytes with biological properties resembling those in vivo can be grown in vitro.

In order to provide a substrate for medical applications, which may be freely elastically deformed depending on a shape of a defective portion or hemostasis portion in a living body, is biocompatible and suitable for tissue regeneration or cell proliferation, or hemostasis, and can be easily manufactured. A collagen substrate of the present invention includes a three-dimensional mesh formed of spun collagen filaments. Preferably, the collagen substrate of the present invention includes cotton formed of spun collagen filaments.

The present invention relates to a method of generating an artificial blood vessel organoid, comprising providing stem cells capable of vascular differentiation, stimulating mesoderm differentiation in said stem cells, stimulating vascular differentiation in said stem cells, developing a cell aggregate from said stem cells, embedding said cell aggregates in a collagenous 3D matrix and stimulatingvascular differentiation of the aggregate in said collagenous 3D matrix; organoids obtainable from said method, uses of said methods and organoids in manipulation and screening studied and kits for performing the methods.

The invention provides a controllable-degradation lacrimal passage suppository and a preparation method thereof. The lacrimal passage suppository is a novel slippage-preventing absorbable lacrimal passage suppository having multiple shapes and multiple degradation times and obtained with a cross-linked collagen matrix as a main raw material and through homogenization, freeze-drying molding and other processing steps. The multiple designed shapes not only all can be convenient to implant in surgery, but also can effectively prevent slippage; in addition, after implantation, the lacrimal passage suppository is swelled due to absorption of water, so that the stability of the product after implantation is more ensured, and the security is extremely high. In addition, with a specific micro-pore structure of the product, epiphora and other complications appearing after the product is implanted can be avoided. The method is simple in process and strong in operability. The prepared product can effectively relieve or treat xerophthalmia.

The invention provides a preparation method of a regional function specific clinical periodontal defect repair module. The module comprises an alveolar bone regeneration functional domain, a periodontal ligament regeneration functional domain and a barrier membrane functional domain. The method comprises the following steps of firstly, carrying out 3D printing on the personalized magnesium-doped wollastonite alveolar bone regeneration functional domain according to clinical alveolar bone defects; and taking MNBG-modified improved PCL / gelatin electrospun membrane as the periodontal ligament regeneration functional domain and Si-HPMC / MA-CMCS hydrogel formed by photocuring as the barrier membrane functional domain, and integrating the alveolar bone regeneration functional domain, the periodontal ligament regeneration functional domain and the barrier membrane functional domain. Imitation periodontal fiber guide structure design and MNBG modification of the periodontal ligament regeneration functional domain provide guidance and support for secretion and integration of collagen matrixes, and MNBG modification can induce proliferation and vertical migration of periodontal ligament fibroblasts, so that vertical increment of periodontal ligament tissue in a defect area is improved, the regeneration of a periodontal tissue complex is promoted, the determinacy of periodontal tissue regeneration is improved, and the technical sensitivity of a periodontal regeneration operation is reduced.

The invention discloses a method for preparing a collagen substrate material for an oral cavity by using biological bone. The method comprises the steps of cutting young bovine bone or swine bone, then, carrying out treatments such as virus removing, defatting, deproteinization, decalcification, cell removing and endotoxin removing, then, carrying out freeze-drying, and then, shearing the bone into required specifications. The whole process is simple, the production cycle is short, and thus, large-scale mass production is facilitated. According to the method, the young bovine bone or swine bone is selected as a raw material and is rich in source, degradation-induced inflammatory reactions are reduced, and clearing is easy, so that immunological rejections caused after human body implantation are avoided. Moreover, the young bovine bone and swine bone materials have good porosity and pore size channels and are similar to human bones in porosity, a human body microenvironment is simulated, crawling passing of seed cells such as osteoblasts is facilitated, healing of soft tissue can be excellently promoted, and the osteogenesis capability is relatively good. Furthermore, chemical reagents of all treatment steps are spin-dried in a long running water state and washed off, the speed is high, and the washing is thorough and residual-free, so that the safety of use of the product is further guaranteed.

Collagen matrix granulate blend, and process for making and using a collagen matrix or granulate blend including collagen and particles of a biphasic calcium phosphate / hydroxyapatite (CAP / HAP) bone substitute material having a sintered CAP core and having its total external surface covered by at least one closed epitactically grown layer of nanocrystalline HAP deposited on the external surface of the sintered CAP core, whereby the epitactically grown nanocrystals have the same size and morphology as human bone mineral, wherein the closed epitactically grown layer of nanocrystalline HAP is transformed from the CAP on the external surface of the sintered CAP core has a non-homogeneous external surface comprising individual clusters of flat crystal platelets consisting of epitactically grown HAP nanocrystals and coarse areas between the individual clusters, whereby the percentage of the coarse areas between the individual clusters as measured by SEM is at least 20% of the total surface.

Apparatus and methods are provided for detecting and monitoring proteolysis of protein matrices such as fibrin clots, extracellular matrix and collagen matrix. The apparatus and methods involve in general the measurement of diffusion-limited electrochemical currents generated by an electroactive species or elactomer in a synthetic protein matrix. The apparatus and methods are applied in various embodiments to detect and monitor the proteolytic activities of a sample, and more specifically fibrinolytic activities and collagenase activities of a sample. The apparatus and methods are utilized generally in the monitoring and diagnostics of cardiovascular, cerebralvascular, and oncology conditions.