34 results about "Linking collagen" patented technology

The invention discloses a collagen-based and high-transparency matrix for cornea thickening and a preparation method of the collagen-based and high-transparency matrix. The preparation method comprises the following steps: assembling a collagen composite solution into a collagen matrix implant on a set curvature mold through electrochemical deposition; the collagen composite solution is prepared from collagen, glacial acetic acid, hydrogen peroxide, heparin and water; putting the obtained collagen matrix implant and a mold into a cross-linking agent solution for cross-linking; cleaning the residual cross-linking agent after completion, separating the collagen matrix implant from the mold, and placing the collagen matrix implant under an ultraviolet light source for cross-linking to obtain a double-cross-linked collagen matrix implant; and soaking the double-crosslinked collagen matrix implant in a nerve growth factor solution, and performing absorption and combination to obtain the collagen-based and high-transparency matrix for cornea thickening. According to the invention, an electro-deposition assembly technical scheme is adopted, the diameter and thickness of collagen assembly fibers are adjusted and controlled through electric field energy, and the collagen assembly fibers are orderly and regularly arranged, so that scattering and refraction of light are reduced, the light transmittance of cornea is increased and can reach more than 90%, and the problem of light blocking caused by materials is effectively avoided. Operation is matched with femtosecond equipment, operation is easy, consumed time is short, and trauma is small.

The invention provides an irradiation crosslinking collagen fiber material as well as a preparation method and application thereof, relates to the field of organic high-molecular compound processing and also relates to the technical field of medical materials. According to the preparation method of the irradiation cross-linked collagen fiber material, for the problem that protein is excessively and rapidly cross-linked in the irradiation process, the excitation rate of electron beams to water molecule free radicals is controlled by changing a solvent system of collagen fibers, introducing part of organic solvent and reducing the moisture content; therefore, the crosslinking degree of irradiation to the collagen material is controlled; furthermore, uniform crosslinking of the collagen fibers is realized by introducing a dispersing agent and controlling the irradiation dose, the irradiation temperature, the material thickness and other processes; and uniform plasmid is carried out on the collagenous fibers by a homogenizing process after irradiation, so that the injectability of the material is realized, and the physical and chemical properties and enzymolysis resistance of the obtained irradiation cross-linked collagenous fiber material are greatly improved.

The invention belongs to the technical field of 3D printing, and particularly relates to dual-crosslinking collagen DLP 3D printing bio-ink, a preparation method and application, the bio-ink comprises the following components: 0.5%-1% m / v of methacrylic acid collagen, 0.1%-0.5% m / v of a photoinitiator, 0.05%-0.5% m / v of an ultraviolet absorbent and 0.001%-0.01% m / v of DPPA; the bio-ink disclosed by the invention has good flowability and light curing performance; the hydrogel for 3D printing by the bio-ink has high mechanical property and thermal stability, and good biocompatibility and biological activity; ordered collagen deposition can be remarkably promoted, epidermis regeneration is promoted, and the repairing speed is increased; the double-crosslinked collagen bio-ink is widely applied to the field of tissue engineering for preparing skin repair materials and osteochondral repair materials.

A bioprosthetic device includes a frame and a bioprosthetic tissue including collagen and a plurality of holes. The bioprosthetic tissue is attached to the frame. The bioprosthetic device includes a plurality of sutures extending through the plurality of holes. In aspects, a periphery of the plurality of holes has a greater collagen cross-linking density or elastic modulus than a bulk of the bioprosthetic tissue. The bioprosthetic device can be a prosthetic heart valve assembly. Alternatively, a collagen-containing scaffold can have predetermined location with has a greater collagen cross-linking density or elastic modulus than a bulk of the collagen-containing scaffold. Methods include applying a cross-linking agent to a predetermined location. Methods further include cross-linking collagen at the predetermined location by impinging the predetermined location\ with a light beam, but a majority of a surface area of the bioprosthetic tissue does not have collagen cross-linked by the impinging.

The invention relates to the technical field of collagen, and provides cross-linked collagen and a preparation method and application thereof in the field of medical cosmetology, the preparation method of the cross-linked collagen comprises the following steps: collagen and an oxazoline compound are subjected to a cross-linking reaction under the condition that the pH value is 5-8, glycine is added to terminate the reaction after the reaction is finished, and the cross-linked collagen is obtained. And then cleaning, centrifuging and drying to obtain the cross-linked collagen. The oxazoline compound is subjected to ring-opening reaction with carboxyl (such as aspartic acid and glutamic acid) and amino (such as lysine and arginine) on a side chain of collagen to form a stable amido bond or similar covalent bond structure, so that efficient crosslinking is realized; and the oxazoline compounds do not contain free aldehyde groups, so that toxic by-products are not generated in the reaction process, and the unification of'high efficiency 'and'safety' is realized.

The invention relates to injectable cross-linked collagen gel and a preparation method thereof. Two crosslinking strategies of self-crosslinking and crosslinking agent crosslinking are fused together for the first time, and two types of amido bonds, namely amido bonds of a collagen-collagen structure (A) and amido bonds of a collagen-fatty amine-collagen structure (B), are respectively constructed through two crosslinking paths, namely catalysis of collagen polypeptide self-crosslinking and catalysis of fatty amine crosslinking, so that a hard / soft dual crosslinking network is obtained. The relative proportion of the structure A and the structure B in the final product is adjusted through a proper cross-linking process, so that the strength and the stability of the final product are adjusted and controlled, and the use requirements of different injection parts can be met.

The invention provides oxidized sanguisorba officinalis cross-linked collagen hemostatic sponge and a preparation method thereof.The preparation method comprises the steps that firstly, sanguisorba officinalis extract is oxidized with sodium periodate, dialyzed and freeze-dried into powder, then the powder is dissolved with an acetic acid solution, a collagen solution is dropwise added, dialyzed and freeze-dried, and an oxidized sanguisorba officinalis cross-linked collagen sponge product is obtained; due to the introduction of the oxidized sanguisorba officinalis extract, the blood coagulation effect of collagen is effectively promoted, the endogenous blood coagulation effect of collagen can be remarkably improved, and the collagen has the capacity of inducing cell adhesion, proliferation and growth and meets the use requirements of medical materials.

The invention discloses a cross-linked collagen implant containing lidocaine and a preparation method of the cross-linked collagen implant, and mainly relates to the technical field of collagen. Compared with the prior art, the cross-linked collagen implant containing lidocaine has the advantages that natural collagen is modified through chemical cross-linking, and the collagen implant which is safe, durable, low in glutaraldehyde residue and low in cytotoxicity and contains anesthetic components is prepared by combining freeze-drying and sterile production processes. The invention also provides an application method of the lidocaine-containing cross-linked collagen implant, which is characterized in that the lidocaine-containing cross-linked collagen implant is injected into a corium layer and is used for correcting moderate and severe nasolabial cleavage wrinkles.

A composition comprising glucuronic acid-1-phosphate, tetracalcium phosphate, cross-linked collagen and water. The composition has preferably the weight ratio Liquid / Powder of 0.18. The composition is used in producing a bone graft and in other dental and medical applications. A process for producing the composition.

The invention discloses a fibroblast preparation and a preparation method and application thereof, and belongs to the technical field of biomedical engineering, and the fibroblast preparation is prepared by mixing and compounding fibroblasts, a carrier and an active substance solution. The preparation method comprises the following steps: S1, cell pretreatment; s2, preparing a cross-linked collagen fiber hydrogel; s3, preparing an active substance solution; and S4, preparing the fibroblast preparation. By pretreating fibroblasts, activating the anti-inflammatory phenotype of the fibroblasts, promoting the fibroblasts to secrete collagen, loading the cells through collagenous fiber hydrogel and adding active substances, the skin lesion is repaired, meanwhile, type II immune excessive activation is inhibited, and the clinical disease performance of eczema mice is remarkably improved. The problems of limited skin disease treatment and skin lesion repair effects caused by low cell survival rate, poor carrier biocompatibility and poor active substance synergism in the prior art are solved, and the preparation method has the potential of preparing drugs for treating skin diseases.

The invention discloses a preparation method of collagen hydrogel crosslinked by oxidized hyaluronic acid. The preparation method comprises the following steps: pretreating collagen; performing high-pressure homogenization to prepare collagen homogenate; the sodium hyaluronate is oxidized and modified by sodium periodate; and carrying out a cross-linking reaction on the oxidized hyaluronic acid and the collagen homogenate to form cured hydrogel, and compounding the cured hydrogel with sulfonated chitosan and coating the cured hydrogel with trehalose to obtain the collagen hydrogel. The collagen is subjected to pretreatment and high-pressure homogenization, so that on the basis of ensuring a three-helix structure of the collagen, the particle size of collagen fibers can be reduced, the steric hindrance of a cross-linking reaction can be reduced, and the cross-linking reaction is more efficient, controllable and uniform. Through triple hydration network design, an HA hydrophilic group is introduced through aldehyde group-amino crosslinking, a sulfonic group anchors water molecules through ionic bonds, the proportion of bound water is increased, after trehalose coating, a glassy hydration layer is formed, the migration rate of the water molecules is reduced, and the problem that a traditional collagen implant is dehydrated and falls back within 1-2 weeks is solved.

A composition comprising a compound of the Formula (Ia), tetracalcium phosphate, a cross-linked collagen and water. The composition has preferably the Liquid / Powder ratio of 0.21. The composition is for use in producing a bone graft and for use in other dental and medical applications. A process for producing the composition.

The application discloses a multifunctional gradient structure artificial skin and a preparation method and application thereof, and belongs to the technical field of biomaterials and biomedical engineering. The upper layer of the application is a silica gel layer, which is used for simulating epidermis to keep a moist environment of a wound and simultaneously plays a barrier role; the intermediate layer is a degradable polymer / natural bioactive protein composite layer, which is used for enhancing mechanical properties; and the lower layer is a hydrogel layer, which is formed by cross-linking hyaluronic acid and carboxymethyl chitosan to form a Schiff base bond and then cross-linking collagen through catalysis. The composition of the hydrogel layer is a component of an extracellular matrix, and the three-dimensional network structure formed is beneficial to cell growth, can simulate a dermis layer to play a function of promoting tissue regeneration and remodeling, and finally the artificial skin with good mechanical strength, capable of preventing wound infection and promoting wound healing is obtained.

The invention discloses a method for preparing a cross-linked implant. According to the embodiment of the invention, the method comprises the following steps: performing cross-linking treatment on collagen in the presence of a cross-linking agent so as to obtain the cross-linked implant, wherein the cross-linking agent is N-hydroxy succinimide and 1-ethyl-(3-dimethylaminopropyl) carbodiimide, and the cross-linking agent is a mixture of N-hydroxy succinimide and 1-ethyl-(3-dimethylaminopropyl) carbodiimide; the collagen is selected from at least one of natural collagen and recombinant collagen; the recombinant collagen is selected from at least one of recombinant I-type human collagen and recombinant III-type human collagen. The method can be used for large-scale production, is simple and easy to implement, can be used for preparing cross-linking implants with various concentrations, and has the characteristics of good viscoelasticity, enzymolysis resistance, high cross-linking degree, high melting point, no cross-linking agent residue and the like.

The application is suitable for the technical field of collagen materials, and provides a CS-HA nanoparticle crosslinked collagen protein shield and a preparation method thereof.The application proposes that type I collagen protein extracted from bovine Achilles tendon is used as a shield preparation material, and CS-HA nanoparticles are used as a crosslinking agent after being activated by EDCM, so that multiple crosslinking sites can be provided, crosslinking efficiency is increased, and the advantages of no crosslinking agent residue, high biological safety and the like are achieved.In addition, the CS-HA nanoparticles can effectively promote the repair of corneal injury, inhibit bacteria and improve the bioavailability of delivered drugs.On this basis, the added PRAM2 plays pharmacological effects such as anti-inflammatory and antioxidant effects.The application realizes the performance improvement of the traditional collagen protein shield, plays the advantages of the collagen protein shield as a corneal bandage, and has a wide application prospect.

The present invention relates to a sandwich immunoassay for determining cross-linked collagen type V in a biological sample, and its use in identifying patients suffering from conditions associated with fibrosis, such as ankylosing spondylitis, inflammatory bowel disease, psoriasis and atopic dermatitis. The invention also relates to a kit for performing a sandwich immunoassay.

The invention discloses an oxidized sodium alginate cross-linked collagen suture line and a preparation method thereof. The preparation method comprises the following steps: by taking nutria tail tendons as raw materials, removing immunogenic components by virtue of synergistic treatment of pepsin and trypsin, and cross-linking with an oxidized sodium alginate solution through a Schiff base covalent bond, so as to form the oxidized sodium alginate cross-linked collagen suture line. Oxidized sodium alginate is innovatively adopted as a cross-linking agent, on one hand, Schiff base covalent bonds are formed through aldehyde groups of the oxidized sodium alginate and amino groups of collagen molecules, so that the mechanical property is enhanced, on the other hand, an energy dissipation network is constructed through dynamic interaction among polysaccharide molecules, and the stretching resistance and enzymolysis resistance of the suture line are remarkably improved. The obtained oxidized sodium alginate crosslinked collagen suture line has excellent mechanical performance, degradation resistance and good biocompatibility, and a new strategy is provided for development of absorbable suture materials.

A composition comprising glucuronic acid- 1 -phosphate, tetracalcium phosphate, cross-linked collagen and water. The composition has preferably the weight ratio Liquid / Powder of 0.18. The composition is used in producing a bone graft and in other dental and medical applications. A process for producing the composition.

The invention discloses a GCF / SGNS-SiO2 double-framework composite film and a preparation method thereof, and the method comprises the following steps: adding sulfonated graphene into a suspension of cross-linked collagenous fiber to prepare a cross-linked collagenous fiber composite sulfonated graphene suspension; carrying out directional freeze-drying treatment on the crosslinked collagen fiber composite sulfonated graphene suspension to prepare crosslinked collagen fiber composite sulfonated graphene aerogel; the preparation method comprises the following steps: adding silicon dioxide into a suspension of cross-linked collagenous fibers to prepare a cross-linked collagenous fiber composite silicon dioxide suspension, dipping cross-linked collagenous fiber composite sulfonated graphene aerogel into the cross-linked collagenous fiber composite silicon dioxide suspension, and then freeze-drying to prepare double-skeleton structure aerogel; and applying pressure to the double-skeleton structure aerogel to prepare the double-skeleton film. The method effectively improves the charge density of the flexible sensor through the synergistic effect of the vertical orientation inner skeleton and the inner and outer skeletons, reduces the interface transmission loss, and improves the stability.

This invention belongs to the field of biomedical materials technology, specifically relating to a highly biocompatible and highly stable collagen fiber, its preparation method, and its application. The method includes the following steps: (1) constructing collagen fibers; (2) cross-linking collagen fibers with a cross-linking agent; (3) dialysis, homogenization, centrifugation, and collection of the precipitate. The prepared cross-linked collagen fibers are tightly packed and highly cross-linked, exhibiting good thermal stability and resistance to enzymatic degradation. The cross-linked collagen fibers have high biocompatibility, promoting fibroblast proliferation; they have high biocompatibility, and the inflammatory response caused after implantation is significantly mild and quickly eliminated. No calcification nodes appear after implantation of the cross-linked collagen fibers, demonstrating excellent anti-calcification effects. The cross-linked collagen fiber implant provided by this invention significantly promotes the production of new collagen and can be applied in fields such as implants, artificial skin, hemostatic sponges, scaffold materials, and medical devices, with broad application prospects.

The present invention provides a collagen-conopeptide fusion protein and a preparation method and application thereof, wherein the collagen-conopeptide fusion protein comprises a collagen fragment and a conopeptide fragment connected in sequence from the N-terminus to the C-terminus, the amino acid sequence of the collagen fragment is shown in SEQ ID NO.2, and the amino acid sequence of the conopeptide fragment is shown in SEQ ID NO.3. The collagen-conopeptide fusion protein is a product obtained by coupling the amino acid sequence of the collagen fragment with the conopeptide fragment, and its sequence is shown in SEQ ID NO.1, and it has the biological activity functions of both collagen and conopeptide, and functionally makes up for the defect that the short-term wrinkle removal effect of recombinant collagen is not obvious and the defect that conopeptide does not have a long-term repair function, and the product can be widely used in medicines and cosmetics.

A composition comprising a compound of the Formula (Ia), tetracalcium phosphate, a cross-linked collagen and water. The composition has preferably the Liquid / Powder ratio of 0.21. The composition is for use in producing a bone graft and for use in other dental and medical applications. A process for producing the composition.

The present invention discloses a preparation method of a modified collagen scaffold, its product and application, comprising the following steps: step 1, cross-linking a collagen solution with a glycosylation reagent to obtain a first cross-linked product; step 2, first infiltrating the first cross-linked product in an ultraviolet photosensitive reagent, and then performing ultraviolet cross-linking to obtain a second cross-linked product; step 3, performing low-dose irradiation cross-linking on the second cross-linked product to complete the preparation of a collagen modified scaffold. The present invention proposes a composite physical cross-linking method, which can reduce costs and risks and realize large-scale production, and can protect the natural structure and activity of collagen to the greatest extent. The modified collagen scaffold prepared by the present invention has stable properties, a relatively stable degradation curve and enzyme tolerance, and good physiological hemostatic function.

The present invention discloses a two-component bone-inducing collagen membrane and a preparation method thereof. The collagen membrane is prepared by blending and cross-linking collagen extracted from the skin or tendon tissue of pigs or cattle with bone morphogenetic protein-2, and then injecting the mixture into a prefabricated mold and freeze-drying. The specific preparation method comprises cutting the animal tissue into thin slices, performing enzymatic hydrolysis, filtering, and precipitation to obtain collagen, which is then redissolved, and EDC and NHS cross-linking agents are added in batches. The collagen is then blended with bone morphogenetic protein-2, and finally injection-molded, freeze-dried, and irradiated for sterilization to obtain a bone morphogenetic protein + collagen two-component structure membrane. The collagen membrane not only has excellent spatial maintenance ability, but also can control the amount of BMP added and the degradation time of the product, and has good medical and practical value.

The invention belongs to the technical field of cosmetics, and particularly relates to a composition containing cross-linked collagen, a preparation method of the composition and application of the composition in the aspect of wound repair. The composition is prepared from the following components in percentage by mass: 0.1 to 0.3 percent of mild crosslinking type I collagen, 0.05 to 0.1 percent of type III collagen, 0.1 to 0.2 percent of mussel mucin, 1 to 3 percent of humectant, 1.5 to 3 percent of preservative and the balance of water. According to the preparation method disclosed by the invention, a heterogeneous fiber network is constructed by lightly crosslinked type I collagen and type III collagen prepared by a specific process according to a specific proportion through a step-by-step assembly technology for the first time, and mussel mucin is introduced as a dynamic crosslinking agent; the three-dimensional hydrogel composition with a triple structure of I type collagen skeleton-III type collagen soft network-mussel mucin crosslinking point is formed through self-assembly under physiological conditions, and the composition can provide a more excellent bionic microenvironment for tissue repair, so that the wound repair effect is remarkably improved.

The invention relates to a preparation method of a collagen filling agent for injection, which is characterized in that non-crosslinked collagen, crosslinked collagen and degradable polymer microspheres are compounded and mixed according to a certain proportion to achieve an instant filling effect, and the degradable polymer microspheres play a long-term role. Amino acid components degraded by the collagen in the first and middle stages can become nutrient substances for stimulating the polyester microspheres to generate collagen regeneration. The cross-linked collagen disclosed by the invention is relatively high in yield and less in water loss through a gradient irradiation cross-linking technology. The non-crosslinked collagen gel can provide a nutritional repair microenvironment and a filling effect in the earlier stage, the crosslinked extracellular matrix can provide a repair microenvironment in the middle stage of filling, and degradation of the polyester microspheres in the later stage promotes collagen regeneration, so that the skin has a good microenvironment in the whole repair period, and the skin elasticity is improved.

The present invention provides a radiation-crosslinked collagen fiber material and its preparation method and application, which relate to the field of organic polymer compound processing and also to the field of medical material technology. Among them, in the preparation method of the radiation-crosslinked collagen fiber material, the problem of excessive and rapid crosslinking of proteins caused by the irradiation process is solved by changing the solvent system of the collagen fiber, introducing some organic solvents, and reducing the moisture content to control the excitation rate of the electron beam on water molecule free radicals, thereby controlling the degree of crosslinking of the collagen material by irradiation; further, by introducing a dispersant and controlling the irradiation dose, irradiation temperature, material thickness and other processes, uniform crosslinking of the collagen fibers is achieved; after irradiation, the collagen fibers are homogenized in a homogenization process, achieving the injectability of the material, and significantly improving the physical and chemical properties and enzymatic resistance of the obtained radiation-crosslinked collagen fiber material.

The invention relates to a printed hemostatic product having at least three-dimensions and being made of a stack of layers deposited on one another from a first external layer up to a second external layer, wherein adjacent layers of the stack of layers are joined together, and wherein at least one layer of the stack of layers has at least one portion made from an hemostatic flowable with a composition comprising: non-cross-linked collagen of the fibrillar type comprising a content of fibrous collagen and / or fibrillar collagen of at least 70% by weight relative to the total weight of the collagen; and—at least one monosaccharide. The invention also relates to a method for forming such an hemostatic product with a three-dimensional additive printer, and the use of an hemostatic flowable as a printing ink in such a three-dimensional additive printer.

The present application provides a kind of irradiation crosslinking collagen injection filling agent and its preparation method and purposes.The collagen injection filling agent includes collagen gel microparticle, the collagen gel microparticle has porous structure;The collagen gel microparticle has connected fiber structure, the fiber structure is formed by collagen self-assembly, and the fiber structure includes fiber bundle and / or fiber mesh lamellar structure;The collagen gel microparticle in the collagen injection filling agent of the present application has the fiber structure formed after collagen self-assembly, i.e. collagen is spontaneously assembled from collagen molecule to fiber bundle and fiber mesh lamellar structure, and the degradation rate is delayed.The fiber network structure of the present application has good mechanical support and slow degradation rate, and simulates the three-dimensional microenvironment of natural extracellular matrix (ECM), promotes cell adhesion proliferation and tissue regeneration.The fiber structure of the present application is beneficial to the crawling proliferation of cells, and promotes the regeneration and repair of skin cells.

A method of producing a multifilament collagen fiber bundle involves treating a multifilament support polymer-collagen fiber bundle containing a plurality of collagen monofilaments supported on a support polymer to remove the support polymer from the multifilament support polymer-collagen fiber bundle using a series of liquid media containing a decreasing concentration of the support polymer. The support polymer is more soluble than collagen in a liquid medium, and the gradual removal of the support polymer provides sufficient time for collagen molecules to self-assemble to form fibrils. The resulting multifilament collagen fiber bundle has a D-band structure similar to native collagen, but is offset by 1 / 6 D-band. The multifilament collagen fiber bundle can be cross-linked through ultraviolet rays under the condition of no photoinitiator to form a cross-linked collagen product with excellent tensile strength and Young modulus, and the product is suitable for collagen-based biological materials in the field of tissue repair and regeneration.