63 results about "Linking collagen" patented technology

A method for producing a cross-linked apatite / collagen porous body comprising the steps of gelling and then freeze-drying a dispersion containing an apatite / collagen composite and collagen to form a porous body, and further cross-linking collagen in the porous body, and a cross-linked apatite / collagen porous body produced by this method.

A method for producing a porous body comprising apatite / collagen composite fibers comprising the steps of gelling a dispersion comprising long apatite / collagen composite fibers having an average length of 10-75 mm, short apatite / collagen composite fibers having an average length of 0.05-1 mm, and a liquid; freezing and drying the resultant gel to form a porous body; and cross-linking collagen in the porous body.

In the process of producing a porous body containing a fibrous apatite / collagen composite by gelating a dispersion comprising the fibrous apatite / collagen composite, collagen and water, freeze-drying the resultant gel to form a porous body, and cross-linking collagen in the porous body, a method for controlling the average pore diameter of the porous body by the solidification time of the gel in the freezing step.

A method of cross-linking collagen present in a collagenous tissue comprising contacting the collagenous tissue with an amount of a formaldehyde releasing agent effective to crosslink the collagen is provided. A method of inhibiting loss of structural integrity of a collagenous tissue during transplantation-related transport comprising contacting the collagenous tissue with an amount of a formaldehyde releasing agent effective to inhibit loss of structural integrity of the collagenous tissue is also provided. A composition for ophthalmic administration comprising a formaldehyde releasing agent, sodium bicarbonate, and ophthalmically suitable carriers or excipients is also provided. Finally, a method of altering the refractive power of a cornea comprising contacting the cornea with a formaldehyde releasing agent so as to effect cross-linking in the cornea and thereby alter the refractive power of the cornea is provided.

The invention relates to a triple cross-linking collagen preparation method, which comprises: providing a soluble collagen sample, mixing the collagen sample and a first cross-linking agent to form a single cross-linking collagen, mixing the single cross-linking collagen and a second cross-linking agent to form a double cross-linking collagen, and mixing the double cross-linking collagen and a third cross-linking agent to form the triple cross-linking collagen, wherein the first cross-linking agent, the second cross-linking agent and the third cross-linking agent are separately selected from a group comprising an aldehyde cross-linking agent, an imine cross-linking agent and an epoxide cross-linking agent, the first cross-linking agent is different from the second cross-linking agent, and the third cross-linking agent is different from the first cross-linking agent and the second cross-linking agent.

The invention discloses a preparation method for a paper-making sizing agent, which comprises the following steps: 1) hydrolyzing gelatin to obtain collagen; 2) dissolving the collagen obtained from the step 1) in water to prepare a collagen aqueous solution with the collagen of which the mass concentration is 10-30 percent, adjusting the pH of the collagen aqueous solution to 5.0-10, dropping a cross-linking agent of which the mass is 2-10% of that of the collagen in the collagen aqueous solution, and reacting for 2-4 h at 35-55 DEG C to obtain a cross-linking collagen system; 3) adding a hydrophobic monomer in the cross-linking collagen system obtained from the step 2) under the condition that the mass ratio of the hydrophobic monomer to the collagen is 1: (1-3), adding an initiator 3-5% of the mass of the hydrophobic monomer in the cross-linking collagen system, pre-initiating for 15-30 min, and finally carrying out the grafting reaction for 2-4 h at 65-85 DEG C to obtain a white emulsion, namely the cross-linking-grafting dual modified collagen paper-making sizing agent. Biomass resource collagen is used as a raw material, and the problem of environment pollution is alleviated while the cost is lowered.

The invention discloses a high-purity and high-activity medical collagen of aquatic biological source, which solves the problems of poor mechanical strength, weak anti-degradation ability and easy microbial infection of natural collagen dressings. Collagen is extracted by acid extraction combined with pepsin to remove telopeptides, and the extraction rate is improved by ultra-high pressure treatment and tissue crushing, and through decellularization, impurity removal, degreasing, multiple salting out, dialysis, and sterilization , decolorization and depyrogenation and other series of purification operations to prepare high-purity and high-activity medical collagen. The network-like collagen sponge with uniform pores was prepared by vacuum freeze-drying process, and then the collagen sponge was modified by physical cross-linking and chemical cross-linking to prepare the inner layer, and then combined with chitosan outer film and medical non-woven The cloth-based cloth layer is compounded, and finally the finished composite multi-layer medical dressing is obtained after Co-60 sterilization.

The invention provides a calcium phosphate bioceramic compounded with collagen and its preparation and use method. The preparation method of the calcium phosphate bioceramic compounded with collagen comprises: using collagen solution from low to high concentration on the surface of calcium phosphate ceramic particles Form a cross-linked collagen coating to obtain a cross-linked collagen coating wrapped on the surface of calcium phosphate ceramic particles, then mix with collagen solution and freeze-dry to obtain the precursor of calcium phosphate bioceramic composite collagen, and then use collagen solution to soak Freeze-dry after coating to obtain calcium phosphate bioceramics with collagen coating. The technical solution of the present invention improves the repair ability and degradation performance of the calcium phosphate bioceramics compounded with collagen, avoids aseptic inflammation caused by the shedding of calcium phosphate ceramic particles during clinical use, and utilizes the plasticity of shape and size and The convenience of use solves many problems encountered in the clinical application of bioceramics.