Porated cartilage products

a cartilage and product technology, applied in the field of cartilage products, can solve the problems of suboptimal yield, pain in the joint, and severe restrictions, and the injury of the articular cartilage remains one of the major unsolved problems,

Inactive Publication Date: 2014-01-16
OSIRIS THERAPEUTICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0030]In some embodiments, the cartilage products of the present invention are not digested. In other embodiments, the cartilage products of the present invention are partially digested by digestive means including enzymatic (e.g. collegenase, pronase, proteinase K, etc. treatment), biochemical (e.g. papain), thermal (e.g. increased heat), chemical (keratin sulfate, tosyllysylchloromethane), mechanical (perforated), any other means of digestion known by those of skill in the art, and combinations of any two or more of the foregoing.

Problems solved by technology

Articular cartilage injury remains one of the major unsolved problems in orthopedics.
However, many of these surgeries yield suboptimal results.
If the lining becomes worn or damaged resulting in lesions, joint movement may be painful or severely restricted.
Whereas damaged bone typically can regenerate successfully, hyaline cartilage regeneration is quite limited.
Although the blood clot introduces inflammatory cytokines, growth factors and MSCs to fill the defect, the process fails to produce articular cartilage and instead stimulates the production of fibrocartilage scar tissue, made from collagen type I. Fibrocartilage has poor long-term biomechanical performance, causes abnormal bone growth, and increases risk of osteoarthritis.
Among other shortcomings, Chen et al. do not teach a flexible cartilage implant or a cartilage implant containing viable native chondrocytes or a non-immunogenic cartilage implant.
Among other shortcomings, Steinwachs et al. do not teach a digested cartilage implant or a non-immunogenic cartilage implant.
Among other shortcomings, Bardos et al. do not teach a digested cartilage sample or a non-immunogenic cartilage sample.
Among other shortcomings, Bravenboer et al. do not teach a cartilage sample comprising a plurality of pores or a non-immunogenic cartilage sample.
Among other shortcomings, Bos et al. do not teach a cartilage sample comprising a plurality of pores or a non-immunogenic cartilage sample.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

Isolation of Femoral Condyles and Tibial Plateau

[0232]A human knee joint was obtained, as depicted in FIG. 1.

[0233]The outer surfaces of the knee joint were cleaned with iodine (10% povidione-iodine solution, Purdue, “Betadine”) without contacting the cartilage with iodine. The knee joint was dissected to separate the femur, tibia and fibula without damaging the cartilage surfaces. Soft tissue (adipose, muscle, fascia, ligaments and tendons) were removed to expose the articular cartilage surfaces on tibial plateau and femoral condyles.

[0234]The portions containing the articular cartilage (tibial plateau and the condyles of the femur) were chilled by placing in chilled saline (0.9% Sodium Chloride irrigation solution, USP) on a cold plate.

example 2

Isolating Cartilage Plugs

[0235]Femur condoyles and tibial plateau were obtained as detailed in Example 1. Osteochondral plugs having diameters of about 1 cm or about 2 cm were obtained from the femur condoyles and tibial plateau. During isolation of the plugs, the condoyles and tibial plateau were kept moist and chilled by periodic immersion in chilled saline or wiped with a wipe soaked in chilled saline. The isolated plugs were then chilled by placement in chilled saline.

[0236]The osteochondral plugs were obtained using a tissue punch while avoiding any areas of damaged cartilage. Specifically, tissue punches with diameters of 1 cm or 2 cm were used to remove whole plugs of cartilage and underlying bone from the articular surface.

example 3

Isolating a Cartilage Sample from Subchondral Bone and Calcified Cartilage

[0237]Osteochondral plugs were obtained as detailed in Example 2. The subchondral bone and calcified cartilage was removed from the osteochondral plugs to provide cartilage samples in the form of cartilage disks. During this process, the cartilage was chilled periodically with chilled saline to prevent overheating.

[0238]Specifically, each osteochondral plug was held securely and the subchondral bone layer was cut (removed) using a sagittal saw with a bent angle blade from the layer of cartilage. Once the subchondral bone was removed, any remaining bone and calcified cartilage was shaved from the underside of the cartilage discs. To prevent overheating, the tissue was frequently immersed in chilled saline throughout the sawing and shaving process. This process was repeated for each of the cartilage disks.

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Abstract

This invention provides porated cartilage products, methods of producing porated cartilage products, and methods of treating subjects by administering cartilage products. Optionally, the cartilage products are sized, porated, and digested to provide a flexible cartilage product. Optionally, the cartilage products comprise viable chondrocytes, bioactive factors such as chondrogenic factors, and a collagen type II matrix. Optionally, the cartilage products are non-immunogenic.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application claims priority to:U.S. Provisional Application No. 61 / 670,424, entitled “Porated Cartilage Products,” filed on Jul. 11, 2011 bearing Docket No. 25532US01 in the office of McAndrews, Held and Malloy;U.S. Provisional Application No. 61 / 670,434, entitled “Disrupted Cartilage Products,” filed on Jul. 11, 2011 bearing Docket No. 25533US01 in the office of McAndrews, Held and Malloy; andU.S. Provisional Application No. 61 / 670,444, entitled “Methods of Manufacturing Cartilage Products,” filed on Jul. 11, 2011 bearing Docket No. 25534US01 in the office of McAndrews, Held and Malloy; the contents of which are hereby incorporated by reference in their entireties.This application is being co-filed with U.S. and PCT Applications entitled “Disrupted Cartilage Products” bearing Docket Nos. 25533U502 and 25533WO01 in the office of McAndrews, Held and Malloy, respectively; U.S. and PCT Applications entitled “Methods of Manufacturing Car...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K35/32
CPCA61K35/32A61L27/24A61L27/3654A61L27/3687A61L27/3691A61L27/3817A61L27/3852A61L27/54A61L2300/414A61L2430/06A61K38/014A61L27/3612A61L27/56A61L27/50A61L27/3608A61L27/3683A61L2300/64
Inventor YOO, DANA SUEKUANG, JIN-QIANGPADEN, JAIMEMAXSON, SCOTT A.DANILKOVITCH, ALLALOPEZ, ERASMOTOM, SAMSON
Owner OSIRIS THERAPEUTICS
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