35 results about "Collagen matrices" patented technology

The invention belongs to the field of biomedical materials and tissue engineering, and particularly relates to an injectable mineralized collagen bone repair composite material loaded with stem cell recruitment factors and osteogenic induction factors and a preparation method of the injectable mineralized collagen bone repair composite material. The composite material is composed of 50%-53% of mineralized collagen matrix particles, 15%-20% of SDF-1alpha loaded heparinized gelatin microspheres, 15%-20% of BMP-2 loaded PDA coated mesoporous silica particles, 8%-10% of an NHS-PEG-NHS cross-linking agent and 4%-6% of glycerin. The composite material can realize time-space sequential regulation and control of'collection first and then differentiation '; a bionic bone matrix microenvironment is provided through the mineralized collagen matrix; the problems of two-factor release conflict and stability are solved; in-vitro experiments show that the material can significantly promote stem cell migration and osteogenic differentiation, and animal experiments prove that the material can recover 82-88% of mechanical properties of bone defect parts. The invention solves the technical problems of insufficient stem cell collection, low osteogenic differentiation efficiency and uncontrollable factor release of the existing bone repair material.

Improved allografts having controlled and consistent properties and useful for soft tissue repair, including breast reconstruction, and other surgical procedures, are disclosed. In one embodiment, an improved acellular dermal matrix (ADM) is produced from a skin sample, where the ADM consists essentially of a single dermal tissue type (e.g., papillary or reticular) and comprises a collagen matrix having substantially uniform density and porosity. In another embodiment, two ADMs are produced from the same dermal tissue type (e.g., papillary or reticular) of the same skin sample, each of which consists essentially of reticular dermis, and comprises a collagen matrix having substantially uniform density and porosity. All such improved ADMs are suitable for use in breast reconstruction and other tissue repair and modification surgery procedures.

The application belongs to the technical fields of nanobiomaterials and cosmetic efficacy materials, and discloses a centella asiatica-derived carbon dot, a preparation method thereof and application thereof in cosmetics. The preparation method of the centella asiatica-derived carbon dot comprises the following steps: step 1, using centella asiatica extract as a carbon source and urea as a nitrogen source, performing a hydrothermal carbonization reaction to obtain a reaction solution; and step 2, performing ultrafiltration purification and freeze-drying on the reaction solution obtained in step 1 to obtain the centella asiatica-derived carbon dot. The centella asiatica-derived carbon dot can penetrate the stratum corneum to achieve deep delivery to the dermis and form protection to the collagen matrix.

The invention relates to the field of biological medicine, and discloses a nano antibody aiming at DDR1, and a sequence and application thereof. The system disclosed by the invention reveals a DDR1-mediated immune escape network, and completes complete development work from a single-target antibody to a multifunctional fusion protein. The research and development of the DDR1nb-Fc and the derivative fusion protein DDR1nb-Fc-mCD80 of the DDR1nb-Fc and the derivative fusion protein DDR1nb-Fc-mCD80 of the DDR1nb-Fc provide a novel treatment strategy with high efficiency and safety for intractable tumors with abundant collagen matrixes.

Disclosed are implantable devices, systems, and methods of fabricating, testing, optimizing, and / or implanting such devices. An implantable device includes a body, a first intrinsic channel network formed in the body and configured to receive cells, and a second intrinsic channel network formed in the body and configured to allow for active flow of a fluid to support viability and / or function of the cells in the first intrinsic channel network. The first intrinsic channel network comprises a first channel and the second intrinsic channel network comprises a second channel, with at least segments of the first and second channels parallel to and spaced apart from each other. Cells may be suspended in a collagen matrix and introduced to the first intrinsic channel network.

A device may include a decellularized biological scaffold, a first electrode, and a second electrode, wherein the decellularized biological scaffold is in electrical and / or chemical communication with the first and second electrodes. In one example, the device is a battery and the device may include an electrolyte layer supported on the decellularized biological scaffold; an anode layer disposed on a first side of the electrolyte layer; and a cathode layer disposed on second side of the electrolyte layer, opposite the anode layer. The electrolyte layer may include a plant-based conductive hydrogel and / or a PEDOT collagen matrix. The anode and / or the cathode layer may comprise metallic vesicles secreted by a plant.

The invention relates to the technical field of nano biological medicines, and discloses a photo-thermal-enzyme catalysis nano-particle as well as a preparation method and application thereof. The photo-thermal-enzyme catalysis nano-particles are constructed by crosslinking water-soluble self-doped polyaniline nano-particles, human serum albumin, lactate oxidase and collagenase through a thioacetal linker capable of being split by active oxygen. Collagenase and lactate oxidase (LOx) are integrated into the same nanoparticle system through a thioacetal (TK) linker, the TK linker is split under the action of tumor microenvironment active oxygen (ROS) and H2O2 generated by catalysis of the LOx, the activity of the two enzymes is synchronously activated, a collagen matrix (physical barrier) is degraded, lactic acid (chemical barrier) is removed, the tumor microenvironment is efficiently remodeled, and the tumor microenvironment can be effectively inhibited. The problems that the regulation effect of a single barrier is limited, and the tumor microenvironment is difficult to improve are solved.

Described is a method of repairing the parenchyma of the brain. The method comprises implanting a biodegradable porous polymeric matrix block, such as a piece of collagen matrix, to a void in the brain through a tubular device. A medical device for use in the method is also described.

Disclosed are implantable devices, systems, and methods of fabricating, testing, optimizing, and / or implanting such devices. An implantable device includes a body, a first intrinsic channel network formed in the body and configured to receive cells, and a second intrinsic channel network formed in the body and configured to allow for active flow of a fluid to support viability and / or function of the cells in the first intrinsic channel network. The first intrinsic channel network comprises a first channel and the second intrinsic channel network comprises a second channel, with at least segments of the first and second channels parallel to and spaced apart from each other. Cells may be suspended in a collagen matrix and introduced to the first intrinsic channel network.

Hydrogels comprising a macromolecular matrix and water may be used for aesthetic fillers, for example, dermal fillers. The macromolecular matrix may include a crosslinked combination of hyaluronic acid and collagen.

The invention discloses a bone-periosteum bionic stent for vascularized bone regeneration and a preparation method of the bone-periosteum bionic stent, and belongs to the technical field of bone repair. The stent comprises a bone bionic stent and a bionic periosteum, the bone bionic scaffold is of a hierarchical pore structure and is formed by compounding polycaprolactone, silk fibroin and beta-tricalcium phosphate through fused deposition 3D printing, and the surface of the bone bionic scaffold is subjected to alkali treatment so as to improve hydrophilicity and osteogenic activity; the bionic periosteum is printed on the surface of the bone bionic scaffold through a near-field direct writing technology and is composed of polycaprolactone, mineralized collagen and magnesium ions, and the mineralized collagen is distributed in the bionic periosteum in a gradient mode. Through integrated bionic design, structures and functions of natural bones and periosteum are simulated; the bone bionic support plays a role in mechanical support and bone formation promotion; the bionic periosteum rapidly releases magnesium ions in the early stage of degradation, and early vascularization is promoted; along with degradation, continuously released collagen induces mineralization of a collagen matrix in the later period; the stent has good cell compatibility and capability of promoting osteogenic differentiation and blood vessel formation.

The invention provides an application of polyclonal Treg cells in treatment of maxillary hypoplasia, and relates to the technical field of biological medicines.The experiment proves that the polyclonal Treg cells can promote the increase of the volume of the middle seam of the maxillary palate; the generation of soft tissues in the middle seam of the maxillary palate can be effectively promoted; the synthesis of a collagen matrix in a palate center seam area can be promoted; the polyclonal Treg cell can be used as an immune cell to be applied to a medicine for treating maxillary dysplasia.

The invention relates to a bionic collagen matrix with a density gradient double-layer structure, and belongs to the technical field of medical materials. The collagen matrix takes telopeptide collagen derived from mammalian dermis or achilles tendon as a main component, and comprises a double-layer structure of a compact layer and a loose layer, and the compact layer is smooth in surface and compact in structure, so that cell migration can be blocked, and a certain protection effect is achieved; the loose layer is of a porous structure, cell ingrowth and blood clot stabilization are facilitated, and a support is provided for tissue repair. The preparation method of the biological membrane comprises the following steps: preparing telopeptide-removed collagen gel, preparing collagen sponge, preparing a collagen tissue regeneration membrane, and compounding the collagen tissue regeneration membrane and the collagen sponge into an integrated double-layer structure through bonding, drying and crosslinking. The double-layer structure simultaneously meets the double requirements of barrier protection and tissue regeneration, the process is simple and controllable, and the double-layer structure is suitable for clinical scenes such as oral soft tissue repair, alveolar ridge reconstruction and tooth extraction pit coverage.

Disclosed are implantable devices, systems, and methods of fabricating, testing, optimizing, and / or implanting such devices. An implantable device includes a body, a first intrinsic channel network formed in the body and configured to receive cells, and a second intrinsic channel network formed in the body and configured to allow for active flow of a fluid to support viability and / or function of the cells in the first intrinsic channel network. The first intrinsic channel network comprises a first channel and the second intrinsic channel network comprises a second channel, with at least segments of the first and second channels parallel to and spaced apart from each other. Cells may be suspended in a collagen matrix and introduced to the first intrinsic channel network.

The application discloses a preparation method of a collagen matrix multifunctional self-power generation heart valve repair material, and specifically comprises the following steps: firstly, stirring and mixing an ammonium sulfate solution, gelatin, acrylic acid and acrylamide, then stirring and adding N, N-methylene bisacrylamide, and then adopting electrostatic spinning to form a film to obtain a power generation layer; secondly, preparing a bonding layer by using sodium carboxymethyl cellulose and a pyrrole monomer; and finally, using a decellularized pig dermal matrix as a support, sequentially bonding the power generation layer and the bonding layer with a chitosan solution, and the heart valve repair material is prepared. The heart valve repair material prepared by the application has the function of self-power generation, can self-generate a micro-current to implement electric stimulation on a heart valve wound site, further promotes wound healing, and can also reduce the risk of infection and pain of a patient.

The application provides a tissue sealing, isolating and repairing matrix and a preparation method thereof, and belongs to the technical field of tissue matrixes.The matrix comprises the following steps: (1) extracting collagen in animal tissue by an enzymatic hydrolysis method; (2) dissolving and preparing a collagen solution by using an acid solution or a phosphate buffer solution; (3) removing air bubbles in the collagen solution by centrifugation or vacuum; (4) laying the defoamed collagen solution in a flat mold, and forming a collagen film after slow and step-by-step dehydration; (5) crosslinking by using a chemical agent or a physical method; (6) soaking and cleaning; (7) slowly and step-by-step removing water to obtain a collagen matrix with a thickness of 5-30 microns; and (8) cutting and packaging, and sterilizing to obtain the tissue sealing, isolating and repairing matrix, which is extremely thin and soft, has strong adhesion and adaptability to a tissue interface, can spontaneously and tightly adhere to a tissue surface, and can effectively prevent air leakage, low-pressure liquid seepage and blood seepage and the like.

The present invention relates to a three-dimensional (3D) co-culture system that mimics the MASLD microenvironment and is used to investigate its effects on hepatocellular carcinoma (HCC). The system comprises hepatic stellate cells embedded in a collagen matrix and HCC cells cultured in a metabolic medium that simulates MASLD conditions. This model enables the investigation of the molecular mechanisms of HCC under laboratory conditions and can be used to identify new therapeutic targets.

Disclosed are implantable devices, systems, and methods of fabricating, testing, optimizing, and / or implanting such devices. An implantable device includes a body, a first intrinsic channel network formed in the body and configured to receive cells, and a second intrinsic channel network formed in the body and configured to allow for active flow of a fluid to support viability and / or function of the cells in the first intrinsic channel network. The first intrinsic channel network comprises a first channel and the second intrinsic channel network comprises a second channel, with at least segments of the first and second channels parallel to and spaced apart from each other. Cells may be suspended in a collagen matrix and introduced to the first intrinsic channel network.

The invention relates to the field of biological medicines, and discloses a nano antibody fusion protein aiming at DDR1, and a sequence and application thereof. The system disclosed by the invention reveals a DDR1-mediated immune escape network, and completes complete development work from a single-target antibody to a multifunctional fusion protein. The research and development of the DDR1nb-Fc and the derivative fusion protein DDR1nb-Fc-mCD80 of the DDR1nb-Fc and the derivative fusion protein DDR1nb-Fc-mCD80 of the DDR1nb-Fc provide a novel treatment strategy with high efficiency and safety for intractable tumors with abundant collagen matrixes.

The present disclosure provides pro-angiogenic proteoglycan mimetics that can provide a provisional, pro-angiogenic scaffold to support tissue regeneration while limiting systemic exposure to VEGF activity. These mimetics can protect a collagen matrix from rapid degradation, and in conjunction with EPCs promote angiogenesis in order to accelerate ischemic wound healing. For example, the provided compounds can be delivered from the end of a catheter following balloon angioplasty to coat the collagen exposed areas, prevent platelet binding and thrombosis, support capture of EPCs from blood to facilitate reendothelialization, and reduce late-lumen loss (neointimal hyperplasia).

Disclosed is a preparation method of a collagen matrix membrane and an application thereof. The invention uses animal intestinal casing as a raw material and sequentially includes steps such as decellularization and degreasing to prepare a collagen matrix membrane with excellent mechanical properties and a long degradation cycle. This membrane is more conducive to providing sufficient bone regeneration space and time during the initial healing phase of periodontal disease, better meeting clinical application needs. Additionally, the collagen matrix membrane prepared by the invention is loaded with multiple growth factors beneficial to periodontal tissue growth, thereby facilitating periodontal tissue regeneration and repair, and improving therapeutic efficacy.

Improved allografts having controlled and consistent properties and useful for soft tissue repair, including breast reconstruction, and other surgical procedures, are disclosed. In one embodiment, an improved acellular dermal matrix (ADM) is produced from a skin sample, where the ADM consists essentially of a single dermal tissue type (e.g., papillary or reticular) and comprises a collagen matrix having substantially uniform density and porosity. In another embodiment, two ADMs are produced from the same dermal tissue type (e.g., papillary or reticular) of the same skin sample, each of which consists essentially of reticular dermis, and comprises a collagen matrix having substantially uniform density and porosity. All such improved ADMs are suitable for use in breast reconstruction and other tissue repair and modification surgery procedures.

This invention discloses a multifunctional SACP-PEP / TMC film, its preparation method, and its applications, belonging to the field of biomaterials technology. The preparation method includes: reacting phosphate and polyacrylic acid in the presence of calcium salt to obtain ACP nanoparticles; dispersing the ACP nanoparticles in a solvent and reacting them with a silane coupling agent to obtain surface-modified SACP nanoparticles; dissolving N,N,N-trimethylchitosan in a solvent, adding the SACP nanoparticles and functional peptides, mixing thoroughly, placing the mixture in a mold, removing the solvent, and molding to obtain the SACP-PEP / TMC film. This invention also discloses the multifunctional SACP-PEP / TMC film prepared by the above method and its applications. This invention, by constructing an antibacterial-antienzyme-remineralization multi-target intervention system, induces orderly remineralization of the collagen matrix while protecting it, achieving comprehensive strengthening of the resin-dentin bonding interface, thus providing an effective technical solution for improving clinical bonding durability.

The utility model relates to the technical field of medical application, and discloses a collagen gel application which comprises a bonding edge, the outer wall of the bonding edge is fixedly connected with an extending bonding part, the inner wall of the bonding edge is provided with a medicine release layer, an auxiliary assembly is arranged above the medicine release layer, and the auxiliary assembly is connected with the extending bonding part. The auxiliary assembly is used for improving the treatment effect of the medicine release layer, and an adsorption assembly is arranged on the inner wall of the bonding edge and used for adsorbing percolate; the auxiliary component comprises a collagen matrix layer, and the collagen matrix layer is arranged above the medicine release layer. According to the utility model, exogenous supplement of recombinant human-derived III-type collagen functional gel is achieved, so that the level of local III-type collagen on the wound surface can be effectively improved, excessive secretion of I-type collagen is inhibited, tissue flexibility recovery is promoted, scar formation is inhibited, pigmentation is reduced, and the clinical treatment effect is improved.

The invention discloses a biocompatible collagen-LM / CNT hydrogel as well as a preparation method and application thereof, and belongs to the field of bioelectronic shielding. According to the present invention, the fish scale derived collagen and liquid metal-carbon nano-tube (LM-CNTs) composite material is subjected to in-situ polymerization so as to develop the super-stretchable and biocompatible hydrogel; according to the fish scale derived collagen hydrogel integrated with the LM-CNT network, the excellent EMI shielding efficiency (the X wave band is 56.33 dB), the ultrahigh stretchability (2230%) and the high conductivity (50.75 S / m) are achieved at the same time. The unique three-dimensional LM-CNT architecture realizes efficient electromagnetic dissipation, while the natural collagen matrix ensures complete biocompatibility, which is crucial to the application of cardiac pacemakers and neural interfaces. The combination of this performance and biosafety makes the material a general solution for the next generation of bioelectronic shielding.

This invention discloses a method for preparing a decellularized matrix with a bioactive coating, belonging to the medical aesthetics industry. The key technical points of the method are the following steps: S1, preparation of the decellularized matrix; S2, treatment of the decellularized matrix; S3, rehydrating the decellularized matrix and then sequentially immersing it in an activation solution, a collagen solution, and another activation solution, followed by washing and freeze-drying to obtain a collagen matrix; S4, adding anti-inflammatory microcapsules and growth factor nanocarriers to a hyaluronic acid solution to obtain a mixed solution; S5, immersing the collagen matrix in the activation solution, reacting and rinsing, coating the mixed solution onto the surface of the collagen matrix, then curing, rinsing, and drying to obtain a decellularized matrix with a bioactive coating. This application improves biocompatibility and safety by constructing a covalently linked hyaluronic acid-collagen bilayer structure on the surface of the decellularized matrix, while also solving the problems of unstable structure and poor mechanical strength of traditional decellularized matrices.

The invention discloses a preparation method of a bio-based film based on natural collagen. The preparation method specifically comprises the following steps: preparing a collagen matrix material; mixing grease and a surfactant to obtain a softening agent; mixing water and polyhydric alcohol to obtain filling liquid; mixing a collagen matrix material, water and the bleaching liquid, adding the mixture into a rotary drum, and rotating; adding the treated collagen matrix material, water and a softening agent into a rotary drum to rotate; and adding the treated collagen matrix material and the filling liquid into a rotary drum, rotating, adjusting the pH value, and drying the collagen matrix. The bio-based film provided by the invention takes natural animal skin as a base material, and the natural animal skin becomes an ideal carrier for loading various materials due to the inherent tissue network and hierarchical structure of the bio-based film. And the collagenous fibers serve as a frame for bearing and dissipating energy, external mechanical energy is effectively distributed through sliding and deformation in the layered structure of the fiber network, and high mechanical strength is achieved. And meanwhile, through filling of the binary solvent and lubrication of the softening agent, the bio-based material is endowed with relatively high toughness.

The present disclosure provides pro-angiogenic proteoglycan mimetics that can provide a provisional, pro-angiogenic scaffold to support tissue regeneration while limiting systemic exposure to VEGF activity. These mimetics can protect a collagen matrix from rapid degradation, and in conjunction with EPCs promote angiogenesis in order to accelerate ischemic wound healing. For example, the provided compounds can be delivered from the end of a catheter following balloon angioplasty to coat the collagen exposed areas, prevent platelet binding and thrombosis, support capture of EPCs from blood to facilitate reendothelialization, and reduce late-lumen loss (neointimal hyperplasia).

A bone implant body serves to be introduced into a bone defect location to promote bone growth in this bone defect location. It has a main body made of a deformable and porous collagen matrix, which contains at least one main body collagen material of a first proportion in the form of a mineralized collagen and a second proportion in the form of a native collagen, and a depot member, which is completely embedded in the main body such that the depot member is completely surrounded by the collagen matrix of the main body and placed centrally within the main body and contains, as a first constituent, at least one depot material in the form of at least one bioactive substance which promotes bone healing. A transition between the collagen matrix of the main body and the depot member is formed by an interfacial area of the depot member.

The invention discloses a plasma substitute surface engineering bioprosthetic valve material and a preparation method thereof, the preparation method comprises the following steps: S1, obtaining a bioprosthetic valve material, and pretreating the bioprosthetic valve material; s2, activating the pretreated bioprosthetic valve material by adopting an amide condensing agent to obtain a carboxyl activated bioprosthetic valve material; s3, grafting the bioprosthetic valve material after the carboxyl is activated by adopting 2-aminoethyl methacrylate hydrochloride to obtain a bioprosthetic valve material grafted with a methacrylate group; and S4, under the action of an initiator, carrying out polymerization reaction on methacrylated hydroxyethyl starch and the bioprosthetic valve material grafted with the methacrylate group, and cleaning to obtain the plasma substitute surface-engineered bioprosthetic valve material. According to the invention, the defects of toxicity, calcification, immunoreaction and the like caused by glutaraldehyde crosslinking of the existing bioprosthetic valve can be overcome, and the defects of thrombus and immunoreaction caused by heterogeneous collagen matrix of the bioprosthetic valve material can be overcome.