56 results about "Collagen matrices" patented technology

The invention discloses a method for widening keratinized gingiva by using a plasma matrix membrane and a preparation method of the plasma matrix membrane, and the method comprises the following steps: determining an expected widening position of the keratinized gingiva based on preoperative CBCT and intraoral scanning data of a patient; root-direction valve reduction is carried out in the area to be widened, and local original keratinized gingiva with the width of 1-2 mm is reserved; preparing a solid plasma matrix and a liquid plasma matrix by adopting a horizontal centrifugal mode, and preparing the plasma matrix membrane by utilizing a plasma matrix pressurizer; and sewing and fixing the obtained plasma matrix membrane in the to-be-widened area. According to the novel operation mode for widening the keratinized gingiva through the plasma matrix, it can be avoided that a second operation area is opened up to obtain an autograft, the postoperative reaction of a patient is reduced, and the economic burden of the patient is relieved; compared with a finished collagen matrix material, the plasma matrix contains autologous bioactive cells and factors and has a better regeneration promoting function.

The invention discloses a preparation method of an acellular matrix containing a bioactive coating, and belongs to the field of medical beauty industry, the key point of the technical scheme is that the preparation method comprises the preparation steps of S1, preparation of the acellular matrix; s2, treating the acellular matrix; s3, rehydrating the decellularized matrix, sequentially immersing the rehydrated decellularized matrix into an activating solution, a collagen solution and an activating solution, reacting, washing the collagen matrix, and freeze-drying to obtain the collagen matrix; s4, adding the anti-inflammatory microcapsules and the growth factor nano-carrier into the hyaluronic acid solution to obtain a mixed solution; s5, the collagen matrix is immersed in an activating solution, after reaction and washing, the surface of the collagen matrix is coated with the mixed solution for reaction, after curing, washing and drying are conducted, and the acellular matrix containing the bioactive coating is obtained.The covalently-connected hyaluronic acid-collagen double-layer structure is constructed on the surface of the acellular matrix, so that biocompatibility and safety are improved; meanwhile, the problems that a traditional acellular matrix is unstable in structure and poor in mechanical strength are solved.

The invention discloses a collagen-based and high-transparency matrix for cornea thickening and a preparation method of the collagen-based and high-transparency matrix. The preparation method comprises the following steps: assembling a collagen composite solution into a collagen matrix implant on a set curvature mold through electrochemical deposition; the collagen composite solution is prepared from collagen, glacial acetic acid, hydrogen peroxide, heparin and water; putting the obtained collagen matrix implant and a mold into a cross-linking agent solution for cross-linking; cleaning the residual cross-linking agent after completion, separating the collagen matrix implant from the mold, and placing the collagen matrix implant under an ultraviolet light source for cross-linking to obtain a double-cross-linked collagen matrix implant; and soaking the double-crosslinked collagen matrix implant in a nerve growth factor solution, and performing absorption and combination to obtain the collagen-based and high-transparency matrix for cornea thickening. According to the invention, an electro-deposition assembly technical scheme is adopted, the diameter and thickness of collagen assembly fibers are adjusted and controlled through electric field energy, and the collagen assembly fibers are orderly and regularly arranged, so that scattering and refraction of light are reduced, the light transmittance of cornea is increased and can reach more than 90%, and the problem of light blocking caused by materials is effectively avoided. Operation is matched with femtosecond equipment, operation is easy, consumed time is short, and trauma is small.

The invention belongs to the field of biomedical materials and tissue engineering, and particularly relates to an injectable mineralized collagen bone repair composite material loaded with stem cell recruitment factors and osteogenic induction factors and a preparation method of the injectable mineralized collagen bone repair composite material. The composite material is composed of 50%-53% of mineralized collagen matrix particles, 15%-20% of SDF-1alpha loaded heparinized gelatin microspheres, 15%-20% of BMP-2 loaded PDA coated mesoporous silica particles, 8%-10% of an NHS-PEG-NHS cross-linking agent and 4%-6% of glycerin. The composite material can realize time-space sequential regulation and control of'collection first and then differentiation '; a bionic bone matrix microenvironment is provided through the mineralized collagen matrix; the problems of two-factor release conflict and stability are solved; in-vitro experiments show that the material can significantly promote stem cell migration and osteogenic differentiation, and animal experiments prove that the material can recover 82-88% of mechanical properties of bone defect parts. The invention solves the technical problems of insufficient stem cell collection, low osteogenic differentiation efficiency and uncontrollable factor release of the existing bone repair material.

The invention discloses a stem cell composition and application thereof in regulating sub-health. The stem cell composition comprises mesenchymal stem cells (MSCs) modified by fusion polypeptide and an anti-IL-6R single domain antibody. The amino acid sequence of the fusion polypeptide is as shown in SEQ ID NO.1, and the fusion polypeptide is modified on the surface of stem cells through click chemistry, so that the chemotactic migration ability, collagen matrix affinity and inflammation tolerance of the stem cells are remarkably improved. The anti-IL-6R single-domain antibody is obtained through phage display screening, the VHH gene of the anti-IL-6R single-domain antibody is shown as SEQ ID NO.2, and the anti-IL-6R single-domain antibody has high affinity and activity of inhibiting an IL-6 / STAT3 signal channel. Animal experiments show that the composition can significantly reduce inflammatory factor (IL-6) and cortisol levels of chronic stress model mice, improve oxidation resistance (SOD) and recover immune homeostasis, and provides an innovative treatment strategy for adjustment of sub-health status.

Improved allografts having controlled and consistent properties and useful for soft tissue repair, including breast reconstruction, and other surgical procedures, are disclosed. In one embodiment, an improved acellular dermal matrix (ADM) is produced from a skin sample, where the ADM consists essentially of a single dermal tissue type (e.g., papillary or reticular) and comprises a collagen matrix having substantially uniform density and porosity. In another embodiment, two ADMs are produced from the same dermal tissue type (e.g., papillary or reticular) of the same skin sample, each of which consists essentially of reticular dermis, and comprises a collagen matrix having substantially uniform density and porosity. All such improved ADMs are suitable for use in breast reconstruction and other tissue repair and modification surgery procedures.

The invention provides a tissue sealing, isolating and repairing matrix and a preparation method thereof, and belongs to the technical field of tissue matrixes. Comprising the following steps: (1) extracting collagen in animal tissues by an enzymolysis method; (2) dissolving with an acid solution or a phosphate buffer solution to prepare a collagen solution; (3) removing bubbles in the collagen solution by adopting centrifugation or vacuum; (4) spreading the defoamed collagen solution in a flat mold, and slowly dehydrating step by step to form a collagen film; (5) cross-linking by adopting a chemical agent or a physical method; (6) soaking and cleaning; (7) slowly removing water step by step to obtain a collagen matrix with a thickness of 5-30 [mu] m; and (8) slitting, packaging and sterilizing to prepare the tissue sealing, isolating and repairing matrix, which is extremely thin and extremely soft in texture, shows extremely strong adhesion and adaptability to the tissue boundary, can be spontaneously and tightly attached to the tissue surface, and effectively prevents the problems of air leakage, low-pressure seepage, errhysis and the like.

The application belongs to the technical fields of nanobiomaterials and cosmetic efficacy materials, and discloses a centella asiatica-derived carbon dot, a preparation method thereof and application thereof in cosmetics. The preparation method of the centella asiatica-derived carbon dot comprises the following steps: step 1, using centella asiatica extract as a carbon source and urea as a nitrogen source, performing a hydrothermal carbonization reaction to obtain a reaction solution; and step 2, performing ultrafiltration purification and freeze-drying on the reaction solution obtained in step 1 to obtain the centella asiatica-derived carbon dot. The centella asiatica-derived carbon dot can penetrate the stratum corneum to achieve deep delivery to the dermis and form protection to the collagen matrix.

The invention relates to the field of biological medicine, and discloses a nano antibody aiming at DDR1, and a sequence and application thereof. The system disclosed by the invention reveals a DDR1-mediated immune escape network, and completes complete development work from a single-target antibody to a multifunctional fusion protein. The research and development of the DDR1nb-Fc and the derivative fusion protein DDR1nb-Fc-mCD80 of the DDR1nb-Fc and the derivative fusion protein DDR1nb-Fc-mCD80 of the DDR1nb-Fc provide a novel treatment strategy with high efficiency and safety for intractable tumors with abundant collagen matrixes.

Disclosed are implantable devices, systems, and methods of fabricating, testing, optimizing, and / or implanting such devices. An implantable device includes a body, a first intrinsic channel network formed in the body and configured to receive cells, and a second intrinsic channel network formed in the body and configured to allow for active flow of a fluid to support viability and / or function of the cells in the first intrinsic channel network. The first intrinsic channel network comprises a first channel and the second intrinsic channel network comprises a second channel, with at least segments of the first and second channels parallel to and spaced apart from each other. Cells may be suspended in a collagen matrix and introduced to the first intrinsic channel network.

A device may include a decellularized biological scaffold, a first electrode, and a second electrode, wherein the decellularized biological scaffold is in electrical and / or chemical communication with the first and second electrodes. In one example, the device is a battery and the device may include an electrolyte layer supported on the decellularized biological scaffold; an anode layer disposed on a first side of the electrolyte layer; and a cathode layer disposed on second side of the electrolyte layer, opposite the anode layer. The electrolyte layer may include a plant-based conductive hydrogel and / or a PEDOT collagen matrix. The anode and / or the cathode layer may comprise metallic vesicles secreted by a plant.

The present invention discloses a collagen matrix flap covalently bound to fibroblast growth factor and a preparation method thereof. The collagen matrix flap is prepared by cross-linking oxidized sodium alginate (OSA) and bovine type I collagen. Subsequently, polyamide-amine dendrimer (PAMAM) is used as a connection carrier to covalently graft basic fibroblast growth factor (bFGF), thereby constructing a scaffold system capable of long-term sustained release of growth factors. The scaffold has good biocompatibility. Experiments have shown that the scaffold of the present invention exhibits clinical operability, superior biological activity to other growth factor-loaded scaffolds, and a more sustained growth factor release effect.

The invention relates to the technical field of nano biological medicines, and discloses a photo-thermal-enzyme catalysis nano-particle as well as a preparation method and application thereof. The photo-thermal-enzyme catalysis nano-particles are constructed by crosslinking water-soluble self-doped polyaniline nano-particles, human serum albumin, lactate oxidase and collagenase through a thioacetal linker capable of being split by active oxygen. Collagenase and lactate oxidase (LOx) are integrated into the same nanoparticle system through a thioacetal (TK) linker, the TK linker is split under the action of tumor microenvironment active oxygen (ROS) and H2O2 generated by catalysis of the LOx, the activity of the two enzymes is synchronously activated, a collagen matrix (physical barrier) is degraded, lactic acid (chemical barrier) is removed, the tumor microenvironment is efficiently remodeled, and the tumor microenvironment can be effectively inhibited. The problems that the regulation effect of a single barrier is limited, and the tumor microenvironment is difficult to improve are solved.

Described is a method of repairing the parenchyma of the brain. The method comprises implanting a biodegradable porous polymeric matrix block, such as a piece of collagen matrix, to a void in the brain through a tubular device. A medical device for use in the method is also described.

The main purpose of this technology is developing a collagen matrix or membrane with antimicrobial properties, comprising collagen from fetal amniotic membranes and metallic nanoparticles. Additionally, its manufacturing procedure is described.

Collagen matrices of varying charge and cross-linking density are disclosed, and methods for preparing such collagen matrices. The cross-linking methods for collagen impart charge and allow for variable cross-linking density within the collagen device. Such variable cross-linking density imparts variable collagen resorption time, and the variable charge allows sequestering and then releasing therapeutics in a controlled manner. The techniques may be used to impact in vitro mineralization via the charged collagen platform by its interaction with rhBMP-2. The techniques can be used to create a positively charged collagen skin substitute that resists infection and supports wound closure.

Collagen matrices of varying charge and cross-linking density are disclosed, and methods for preparing such collagen matrices. The cross-linking methods for collagen impart charge and allow for variable cross-linking density within the collagen device. Such variable cross-linking density imparts variable collagen resorption time, and the variable charge allows sequestering and then releasing therapeutics in a controlled manner. The techniques may be used to impact in vitro mineralization via the charged collagen platform by its interaction with rhBMP-2. The techniques can be used to create a positively charged collagen skin substitute that resists infection and supports wound closure.

Disclosed are implantable devices, systems, and methods of fabricating, testing, optimizing, and / or implanting such devices. An implantable device includes a body, a first intrinsic channel network formed in the body and configured to receive cells, and a second intrinsic channel network formed in the body and configured to allow for active flow of a fluid to support viability and / or function of the cells in the first intrinsic channel network. The first intrinsic channel network comprises a first channel and the second intrinsic channel network comprises a second channel, with at least segments of the first and second channels parallel to and spaced apart from each other. Cells may be suspended in a collagen matrix and introduced to the first intrinsic channel network.

The invention discloses a sampling detection device for collagen matrix preparation, and relates to the field of collagen matrix sampling, the sampling detection device comprises a base, a rotating seat, a mechanical arm and an air cylinder, the rotating seat driven by a motor is installed on the base, the mechanical arm is fixed on the rotating seat, the air cylinder is fixed on the mechanical arm, and the air cylinder is fixed on the rotating seat. A limiting mechanism is fixed to the output end of the air cylinder, a sampling mechanism is fixed to the lower end of the limiting mechanism, and a scraping mechanism is arranged on the outer side of the sampling mechanism. According to the sampling detection device for collagen matrix preparation, after sampling is completed and a sampling mechanism is separated from the liquid level of a collagen matrix, a convex shaft is driven to move through contraction of an air cylinder, and a scraping plate rotates around the outer wall of a sampling barrel in cooperation with the sliding effect between the convex shaft and a sliding groove spiral structure, so that the effect of scraping the collagen matrix on the outer wall of the sampling barrel is achieved; waste of the collagen matrix adhered to the outer wall of the sampling barrel is avoided, manual treatment is not needed in the whole operation, and the use convenience of the device is improved.

Hydrogels comprising a macromolecular matrix and water may be used for aesthetic fillers, for example, dermal fillers. The macromolecular matrix may include a crosslinked combination of hyaluronic acid and collagen.

The invention discloses a bone-periosteum bionic stent for vascularized bone regeneration and a preparation method of the bone-periosteum bionic stent, and belongs to the technical field of bone repair. The stent comprises a bone bionic stent and a bionic periosteum, the bone bionic scaffold is of a hierarchical pore structure and is formed by compounding polycaprolactone, silk fibroin and beta-tricalcium phosphate through fused deposition 3D printing, and the surface of the bone bionic scaffold is subjected to alkali treatment so as to improve hydrophilicity and osteogenic activity; the bionic periosteum is printed on the surface of the bone bionic scaffold through a near-field direct writing technology and is composed of polycaprolactone, mineralized collagen and magnesium ions, and the mineralized collagen is distributed in the bionic periosteum in a gradient mode. Through integrated bionic design, structures and functions of natural bones and periosteum are simulated; the bone bionic support plays a role in mechanical support and bone formation promotion; the bionic periosteum rapidly releases magnesium ions in the early stage of degradation, and early vascularization is promoted; along with degradation, continuously released collagen induces mineralization of a collagen matrix in the later period; the stent has good cell compatibility and capability of promoting osteogenic differentiation and blood vessel formation.

Disclosed herein are embodiments of a reinforced collagen matrix device for superior capsule repair. The device can include a collagen matrix cover, a first reinforcement strip positioned along the first side of the cover, and a second reinforcement strip positioned along the second side of the cover. A first end and a second end of the first and second reinforcement strips can extend past the first end and the second end of the collagen matrix cover so as not be covered by the collagen matrix cover. The collagen matrix cover can be folded along a first fold and a second fold and extend over the first and second reinforcement strips so that a portion of the first and second reinforcement strips are covered by the collagen matrix cover along a portion of the length of the first reinforcement strip.

The invention provides an application of polyclonal Treg cells in treatment of maxillary hypoplasia, and relates to the technical field of biological medicines.The experiment proves that the polyclonal Treg cells can promote the increase of the volume of the middle seam of the maxillary palate; the generation of soft tissues in the middle seam of the maxillary palate can be effectively promoted; the synthesis of a collagen matrix in a palate center seam area can be promoted; the polyclonal Treg cell can be used as an immune cell to be applied to a medicine for treating maxillary dysplasia.

The invention relates to a bionic collagen matrix with a density gradient double-layer structure, and belongs to the technical field of medical materials. The collagen matrix takes telopeptide collagen derived from mammalian dermis or achilles tendon as a main component, and comprises a double-layer structure of a compact layer and a loose layer, and the compact layer is smooth in surface and compact in structure, so that cell migration can be blocked, and a certain protection effect is achieved; the loose layer is of a porous structure, cell ingrowth and blood clot stabilization are facilitated, and a support is provided for tissue repair. The preparation method of the biological membrane comprises the following steps: preparing telopeptide-removed collagen gel, preparing collagen sponge, preparing a collagen tissue regeneration membrane, and compounding the collagen tissue regeneration membrane and the collagen sponge into an integrated double-layer structure through bonding, drying and crosslinking. The double-layer structure simultaneously meets the double requirements of barrier protection and tissue regeneration, the process is simple and controllable, and the double-layer structure is suitable for clinical scenes such as oral soft tissue repair, alveolar ridge reconstruction and tooth extraction pit coverage.

Disclosed are implantable devices, systems, and methods of fabricating, testing, optimizing, and / or implanting such devices. An implantable device includes a body, a first intrinsic channel network formed in the body and configured to receive cells, and a second intrinsic channel network formed in the body and configured to allow for active flow of a fluid to support viability and / or function of the cells in the first intrinsic channel network. The first intrinsic channel network comprises a first channel and the second intrinsic channel network comprises a second channel, with at least segments of the first and second channels parallel to and spaced apart from each other. Cells may be suspended in a collagen matrix and introduced to the first intrinsic channel network.

The invention discloses a bioprosthetic valve material of a bionic endothelial glycocalyx-like hydrogel coating and a preparation method of the bioprosthetic valve material, and the preparation method comprises the following steps: S1, obtaining a biological material, and pretreating the biological material; s2, preparing an activated caffeic acid solution, and soaking the pretreated biological material into the activated caffeic acid solution, so that caffeic acid is grafted on the biological material; and S3, preparing an Alg-DA solution containing an initiator, immersing the grafted biological material into the Alg-DA solution, carrying out an oxidative coupling reaction, and after the reaction is finished, carrying out cleaning so as to obtain the bioprosthetic valve material with the bionic endothelial glycocalyx-like hydrogel coating. The biomimetic endothelial glycocalyx-like hydrogel coating can be constructed on the thrombogenic collagen matrix of the bioprosthetic valve to simulate the good antithrombotic performance of normal blood vessels, and the blood compatibility of the bioprosthetic valve is remarkably improved.

The application discloses a preparation method of a collagen matrix multifunctional self-power generation heart valve repair material, and specifically comprises the following steps: firstly, stirring and mixing an ammonium sulfate solution, gelatin, acrylic acid and acrylamide, then stirring and adding N, N-methylene bisacrylamide, and then adopting electrostatic spinning to form a film to obtain a power generation layer; secondly, preparing a bonding layer by using sodium carboxymethyl cellulose and a pyrrole monomer; and finally, using a decellularized pig dermal matrix as a support, sequentially bonding the power generation layer and the bonding layer with a chitosan solution, and the heart valve repair material is prepared. The heart valve repair material prepared by the application has the function of self-power generation, can self-generate a micro-current to implement electric stimulation on a heart valve wound site, further promotes wound healing, and can also reduce the risk of infection and pain of a patient.

The application provides a tissue sealing, isolating and repairing matrix and a preparation method thereof, and belongs to the technical field of tissue matrixes.The matrix comprises the following steps: (1) extracting collagen in animal tissue by an enzymatic hydrolysis method; (2) dissolving and preparing a collagen solution by using an acid solution or a phosphate buffer solution; (3) removing air bubbles in the collagen solution by centrifugation or vacuum; (4) laying the defoamed collagen solution in a flat mold, and forming a collagen film after slow and step-by-step dehydration; (5) crosslinking by using a chemical agent or a physical method; (6) soaking and cleaning; (7) slowly and step-by-step removing water to obtain a collagen matrix with a thickness of 5-30 microns; and (8) cutting and packaging, and sterilizing to obtain the tissue sealing, isolating and repairing matrix, which is extremely thin and soft, has strong adhesion and adaptability to a tissue interface, can spontaneously and tightly adhere to a tissue surface, and can effectively prevent air leakage, low-pressure liquid seepage and blood seepage and the like.

The present invention relates to a three-dimensional (3D) co-culture system that mimics the MASLD microenvironment and is used to investigate its effects on hepatocellular carcinoma (HCC). The system comprises hepatic stellate cells embedded in a collagen matrix and HCC cells cultured in a metabolic medium that simulates MASLD conditions. This model enables the investigation of the molecular mechanisms of HCC under laboratory conditions and can be used to identify new therapeutic targets.