Preparation method of collagen matrix composite biological film for guided tissue regeneration

A technology for guiding regeneration and composite membrane, applied in medical science, surgery, etc., can solve the problems of loss of space maintenance ability, fast degradation speed, low strength, etc., to achieve good mechanical properties and degradation controllability, good repair, low cost Effect

Active Publication Date: 2013-05-01
GUANGZHOU PUDAO LIANXIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still some problems in the research of collagen membrane as GTR: firstly, the degradation rate in the body is too fast, and secondly, compared with the requirements of GTR membrane, most of the current collagen membrane products are weak in strength, and they are prone to collapse during application and lose space to maintain ability

Method used

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  • Preparation method of collagen matrix composite biological film for guided tissue regeneration
  • Preparation method of collagen matrix composite biological film for guided tissue regeneration
  • Preparation method of collagen matrix composite biological film for guided tissue regeneration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for preparing a collagen-based composite biofilm, comprising the steps of:

[0034] (1) Dissolve collagen in 0.1M hydrochloric acid solution at 25°C to obtain a collagen solution with a concentration of 3mg / ml;

[0035] (2) Slowly adjust the pH value of the solution obtained in step (1) to 10 with 0.1N sodium hydroxide, and keep it for 30 minutes;

[0036] (3) Add bioactive glass (BAG) to the collagen solution in step (2), wherein the weight of BAG is 10% of the dry weight of collagen, and stir with a stirrer to obtain a uniform solution;

[0037] (4) Use hydrochloric acid or sodium hydroxide solution to adjust the pH value of the solution obtained in step (3) to 7, and centrifuge at a speed of 3000r / min for 5 minutes. At this time, the collagen-wrapped BAG precipitates at the bottom of the centrifuge cup to form a film. Remove the supernatant and collect the collagen composite film;

[0038] (5) Forming the collagen composite film collected in step (4), free...

Embodiment 2

[0042] A method for preparing a collagen-based composite biofilm, comprising the steps of:

[0043] (1) Dissolve collagen in 0.1M hydrochloric acid solution at 4°C to obtain a collagen solution with a concentration of 10mg / ml;

[0044] (2) Slowly adjust the pH value of the collagen solution obtained in step (1) to 9 with 0.1N sodium hydroxide, and keep it for 60 minutes;

[0045] (3) Add hydroxyapatite (HA) to the solution obtained in step (2), wherein the weight of HA is 5% of the dry weight of collagen, and stir with a stirrer to obtain a uniform solution;

[0046] (4) Use hydrochloric acid or sodium hydroxide solution to adjust the pH value of the solution obtained in step (3) to 7, and centrifuge at a speed of 4000r / min for 3 minutes. At this time, the collagen-coated HA precipitates at the bottom of the centrifuge cup to form a film, remove The supernatant was collected to collect the collagen composite membrane;

[0047] (5) Forming the collagen composite film collecte...

Embodiment 3

[0052] A method for preparing a collagen-based composite biofilm, comprising the steps of:

[0053] (1) Dissolve collagen in 0.5M acetic acid solution at 4°C to obtain a collagen solution with a concentration of 8 mg / ml;

[0054] (2) Slowly adjust the pH value of the collagen solution obtained in step (1) to 8 with 0.1N ammonia water, and keep it for 5 minutes;

[0055] (3) Add β-tricalcium phosphate (β-TCP) to the collagen solution in step (2), wherein the weight of β-TCP is 1% of the dry weight of collagen, and stir with a stirrer to obtain a uniform solution;

[0056] (4) Adjust the pH value of the solution obtained in step (3) to 7 with acetic acid or ammonia water, centrifuge at a speed of 6000r / min for 3 minutes. Supernatant, collect the collagen composite film;

[0057] (5) Forming the composite collagen membrane collected in step (4), freeze-drying at -20°C, and vacuum thermal crosslinking at 140°C;

[0058] (6) Soak the composite collagen membrane obtained in step ...

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Abstract

The invention discloses a preparation method of a collagen matrix composite biological film for guided tissue regeneration. The method comprises the following steps of dissolving collagen in an acid solution, adjusting pH (potential of hydrogen) of an obtained solution to be alkaline, continuing to add an inorganic powder material, uniformly stirring, adjusting pH of an obtained solution to be neutral, centrifuging, removing a supernate, collecting a collagen composite film, allowing the obtained collagen composite film to be subjected to mold forming, freeze-drying, using a physical or/and chemical method for crosslinking, and obtaining the collagen matrix composite biological film.

Description

technical field [0001] The invention relates to a method for preparing a collagen-based composite biofilm for guiding tissue regeneration. Background technique [0002] Periodontal guided tissue regeneration (guided tissue regeneration, GTR) is currently the most advanced technology in the treatment of periodontal disease in the world. The concept of GTR was first proposed by Nymans et al. (Nyman S.Lindhe J.Karring T, et al. Clin Periodontol.1982; 9(4): 290-296) in 1982. The basic principle is to use the barrier membrane as a space isolation. While preventing gingival connective tissue cells and combined epithelial cells from migrating to the root side, it selectively allows periodontal ligament cells and alveolar bone cells to adhere to the root surface and differentiate to form new periodontal attachments. This approach has been shown to be more effective in achieving clinical attachment and reducing probing depth than open flap debridement in the treatment of endosseous ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/12
Inventor 任力季培红秦晓杰王迎军刘卅
Owner GUANGZHOU PUDAO LIANXIN BIOTECH CO LTD
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