Transcription factor C/EBPZ for regulating adipocyte formation and application thereof

A technology of adipocytes and transcription factors, applied in the field of animal molecular genetics and developmental biology, can solve problems such as naming confusion, confusion, and little understanding of C/EBPZ

Pending Publication Date: 2021-03-12
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] It is worth pointing out that DDIT3 (Gene ID: 1649) was named C/EBPZ for a while, but it is a completely different gene from the current official name C/EBPZ (Gene ID: 10153). A previous naming confusion caused some confusio...

Method used

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  • Transcription factor C/EBPZ for regulating adipocyte formation and application thereof
  • Transcription factor C/EBPZ for regulating adipocyte formation and application thereof
  • Transcription factor C/EBPZ for regulating adipocyte formation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Cloning of chicken C / EBPZ gene and detection of mRNA expression level

[0041] 1. Tissue Sampling

[0042] The fourteenth generation 1-12-week-old broiler chickens of Northeast Agricultural University (Northeast Agricultural University broiler lines divergently selected for abdominal fat content, NEAUHLF) were used as experimental materials, from 1 to 12 weeks of age, slaughtered every week Ten roosters (5 high and low fat). At each week of age, abdominal fat pads were collected after slaughter. At 7 weeks of age, 15 other tissues including liver, duodenum, jejunum, ileum, thorax, leg muscles, perigastric fat, heart, spleen, kidney, pancreas, glandular stomach, brain, and testis were also collected . All collected tissues were immediately frozen in liquid nitrogen and stored in a -80°C freezer for later use.

[0043] 2. RNA extraction

[0044] 1) 100 mg of collected chicken tissue samples were taken, ground in liquid nitrogen, and then 1 mL of Trizol reag...

Embodiment 2

[0070] Example 2 Effects of overexpression of C / EBPZ on the formation of chicken preadipocytes

[0071] 1. Isolation and culture of chicken preadipocytes

[0072] 1) Abdominal adipose tissue (3-5 g) was taken from 12-day-old AA broilers, washed twice with PBS, digested with 2 mg / mL type I collagenase (Sigma) at 37°C for 1 hour, up and down every 10 minutes Mix by inversion once.

[0073] 2) Pass the digested tissue fluid through 100-micron and 600-micron filters to remove undigested tissue pieces.

[0074] 3) Collect the filtered tissue digestion solution and centrifuge at 200g for 10 minutes.

[0075] 4) Let stand for 10 minutes to allow the cells to be stratified. After the cells in the lower layer are treated with red blood cell lysate again, centrifuge at 200 g for 10 minutes. The obtained vascular stromal (SVF) cells derived from chicken abdominal adipose tissue are used as chicken preadipocytes.

[0076] 5) The isolated chicken preadipocytes were suspended in complete...

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Abstract

The invention discloses a transcription factor C/EBPZ for regulating adipocyte formation and application thereof, belongs to the field of animal molecular genetics and developmental biology, and discloses application of the transcription factor C/EBPZ for regulating and controlling chicken adipocyte formation. The invention discloses an expression mode of chicken C/EBPZ in various tissues and an expression rule of C/EBPZ in a chicken adipose tissue growth and development process by utilizing an RT-PCR technology. According to the invention, an overexpression vector of the transcription factoris constructed, and the C/EBPZ transcription factor is verified to regulate and control the differentiation and proliferation of chicken preadipocytes; the invention further discloses an action mechanism of the transcription factor C/EBPZ in chicken adipose tissue formation. The invention provides a novel application of a C/EBPZ transcription factor in regulation and control of chicken adipocyte formation, and the C/EBPZ transcription factor has practical value in the fields of chicken genetic breeding and animal nutrition.

Description

technical field [0001] The invention relates to the fields of animal molecular genetics and developmental biology, in particular to a transcription factor C / EBPZ that regulates the formation of adipocytes and its application. Background technique [0002] After nearly a hundred years of unremitting efforts by breeders, broiler breeding has made great progress. At present, the daily gain and feed conversion ratio of commercial generation broilers have reached a high level. However, excessive abdominal fat accumulation has become an important problem in the current broiler production field due to over-selection of growth traits during long-term breeding. Excessive accumulation of abdominal fat not only affects the production efficiency of broilers and causes feed waste, but also causes sudden death of broilers and deterioration of carcass quality. Breeding high-quality low-fat broilers is an effective means to control excess abdominal fat accumulation in broilers. [0003] ...

Claims

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Application Information

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IPC IPC(8): C12N15/12C12Q1/6888
CPCC07K14/47C12Q1/6888C12Q2600/124C12Q2600/158
Inventor 张志威陈月婵蓸冬冬高玲羽林涛
Owner SHIHEZI UNIVERSITY
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