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50 results about "Dermal papillae" patented technology

In the human skin, the dermal papillae are small, nipple-like extensions of the dermis into the epidermis. At the surface of the skin in hands and feet, they appear as epidermal or papillary ridges. Blood vessels in the dermal papillae nourish all hair follicles and bring nutrients and oxygen to the lower layers of epidermal cells. The pattern of ridges they produce in hands and feet are partly genetically determined features that develop before birth. They remain substantially unaltered throughout life, and therefore determine the patterns of fingerprints, making them useful in certain functions of personal identification. The dermal papillae are part of the uppermost layer of the dermis, the papillary dermis, and the ridges they form greatly increase the surface area between the dermis and epidermis. Because the main function of the dermis is to support the epidermis, this greatly increases the exchange of oxygen, nutrients, and waste products between these two layers. Additionally, the increase in surface area prevents the dermal and epidermal layers from separating from each other by strengthening the junction between them.

Peptides having activity to promote hair growth and inhibit hair loss and uses thereof

The present invention relates to a peptide having an activity of promoting hair growth or preventing or inhibiting hair loss. The peptide of the present invention has an activity of promoting proliferation of hair follicle dermal papilla cells (HFDPC), activating a cell proliferation-related signal protein in human dermal papilla cells, activating β-catenin, improving expression levels of factors downstream of β-catenin, down-regulating expression levels of hair-loss protein DKK-1, and increasing expression of keratins in hair outer root sheath cells (HORSC).
Owner:CAREGEN

Plant lactobacillus CCFM1353 and preparation of anti-hair loss and hair care metaplasma and application of transformed cypress leaves

The application discloses a plant Lactobacillus plantarum CCFM1353, a metapolybiose prepared from the plant Lactobacillus plantarum CCFM1353 for preventing hair loss and promoting hair growth, and application of the metapolybiose in combination with transformed Chinese arborvitae leaves, and belongs to the technical fields of microorganisms and medicines. The plant Lactobacillus plantarum CCFM1353 and the metapolybiose and symbiotic preparation prepared from the plant Lactobacillus plantarum CCFM1353 can promote the expression of IGF-1, VEGF and TGF-beta 2 of hair follicle dermal papilla cells (HDPC), promote hair growth of mice, and promote the expression of VEGF, IGF-1, Wnt10b, Cyclin D1, Ki67 and Bcl2 in the skin of mice. The plant Lactobacillus plantarum CCFM1353 and the metapolybiose and symbiotic preparation prepared from the plant Lactobacillus plantarum CCFM1353 can be used for preparing products for preventing and / or treating hair loss, and have great application prospects.
Owner:JIANGNAN UNIV

Peptide with activity of promoting hair growth and inhibiting hair loss and use thereof

PendingUS20260139003A1Cosmetic preparationsHair cosmeticsDermal papillaeOuter root sheath
The present invention relates to a peptide with the activity of promoting hair growth or preventing or inhibiting alopecia. The peptide of the present invention promotes the proliferation of human follicular dermal papilla cells (HFDPC), activates cell proliferation-related signaling proteins and beta-catenin in human dermal papilla cells, enhances the expression levels of beta-catenin downstream genes, down-regulates the expression levels of the hair loss protein DKK-1, increases the expression of keratin proteins in hair outer root sheath cells (HORSC) and up-regulates the expression of transcription factors involved in cell activation in germinal matrix cells (GMC).
Owner:CAREGEN

Compositions for promoting hair growth, methods of making and using same

PendingCN122351149ACyclodextrinDermal papillae
This invention relates to a composition for promoting hair growth, comprising a dynamically cross-linked hydrogel loaded with dermal papilla cells. The dynamically cross-linked hydrogel includes adamantane-grafted hyaluronic acid, gelatin, and cyclodextrin modified with cross-linking groups. A three-dimensional network of the dynamic hydrogel is formed through host-guest interactions and the cross-linking of the cross-linking groups. The invention also provides a method for preparing the composition for promoting hair growth, comprising mixing adamantane-grafted hyaluronic acid and cyclodextrin modified with cross-linking groups to obtain a hydrogel prepolymer; adding a dermal papilla cell suspension to the hydrogel prepolymer to initiate a cross-linking reaction of the cross-linking groups to form a dynamically cross-linked hydrogel loaded with dermal papilla cells. The composition can be directly delivered to the depth of hair follicles in the scalp via mesotherapy injection to activate dormant hair follicles from the root, promote new hair growth, and thicken existing hair. The hair regeneration effect is rapid and long-lasting.
Owner:SOUTH CHINA UNIV OF TECH

Application of polylactic acid and its copolymers in the preparation of products that promote hair follicle growth and / or repair hair follicle damage

PendingCN122297511ADermal papillaeCopolymer
This invention discloses the use of polylactic acid (PLA) and its copolymers in the preparation of products that promote hair follicle growth and repair, belonging to the field of biomedical technology. Through in vitro and in vivo experiments, this invention demonstrates that PLA can significantly upregulate the expression of lactate dehydrogenase A and transforming growth factor β2 in dermal papilla cells, promoting dermal papilla cell proliferation and hair follicle growth and damage repair. Based on these findings, this invention provides the application of PLA and its copolymers in the preparation of products that promote hair follicle growth and damage repair, offering a safe and effective treatment strategy for hair follicle-related diseases.
Owner:CHANGCHUN SINOBIOMATERIALS CO LTD

Organic acid-containing anti-hair-loss composition based on cacumen biotae extract and application of organic acid-containing anti-hair-loss composition

The invention discloses an organic acid-containing anti-hair-loss composition based on a cacumen biotae extract, which comprises the cacumen biotae extract and an organic acid as anti-hair-loss active components, and the acid is succinic acid or malic acid or a combination of the succinic acid and the malic acid. The invention also provides application of the anti-hair loss composition in regulating hair follicle energy metabolism and preventing hair loss. The composition containing the cacumen biotae extract, the succinic acid and the malic acid can promote the activity of hair papilla cells, enhance the energy metabolism of the cells, recover the function of hair follicles damaged by oxidation and recover the energy metabolism of the hair follicles damaged by oxidation, so that the composition has a good effect of preventing and treating alopecia, stimulates hair growth and has few toxic and side effects.
Owner:GUANGZHOU HUANYA COSMETIC SCI & TECH CO LTD

Traditional Chinese medicine composition for preventing hair loss and growing hair and application thereof

The invention provides a traditional Chinese medicine composition for preventing hair loss and promoting hair growth and application thereof, and relates to the technical field of hair loss prevention and promoting hair growth, the traditional Chinese medicine composition is composed of Chinese doughnut, cockscomb, albizia flower, Chinese honey locust, schizonepeta, lithospermum, polygonum multiflorum, prepared rehmannia root, notopterygium root, radix angelicae pubescentis, ruddle and periostracum cicada; in-vitro experiments show that the traditional Chinese medicine composition can promote proliferation of human dermal papilla cells HDPC, relieve the damage degree of HDPC and induce cycle transformation of hair follicles, so that the effect of promoting hair growth is achieved; animal experiments show that the traditional Chinese medicine composition can significantly increase the number of hair follicles and the thickness of skin and dermis fat of mice, reduce the levels of mouse serum IL-2, TNF-alpha and TGF-beta1 and reduce the levels of testosterone, dihydrotestosterone and estradiol in mouse skin tissue, so that hair follicle fibrosis is inhibited, the growth period of hair follicles is accelerated, the growth period is prolonged, the hair loss phenomenon is improved, and the hair loss rate is increased. The hair growth is promoted.
Owner:HENAN CHENDU BIOTECHNOLOGY CO LTD

Improvement of artificial hair follicle germ

An artificial hair follicle germ 10 has a three-dimensional structure in which a first block 11 including hair follicle epithelial stem cells, a second block 12 including hair papilla cells, and a third block 13 including dermal-derived mesenchymal cells (DDMCs) are stacked in this order. A method for artificially preparing a hair follicle germ 10 includes three-dimensionally culturing a first block 11 including hair follicle epithelial stem cells, a second block 12 including hair papilla cells, and a third block 13 including dermal-derived mesenchymal cells (DDMCs), these blocks being stacked in this order.
Owner:ORGANTECH INC

Dermal dermal papilla cell spheroids and in vitro culture methods, vesicles and uses thereof

The present application belongs to the field of biological materials and regenerative medicine, and particularly relates to a dermal dermal papilla cell sphere, an in-vitro culture method thereof, vesicles and uses. The dermal dermal papilla cell sphere is prepared according to the following method: extracting primary dermal dermal papilla cells from an in-vitro dermal dermal papilla tissue, subculturing for 3-5 generations; inoculating the subcultured dermal dermal papilla cells into an SLF-3D culture system for culture, collecting semi-suspended spheres when the dermal dermal papilla cells grow to a fusion degree of 90-100%, separating the spheres into single cells, and re-inoculating the single cells into the original SLF-3D culture system for culture for 3-5 days, and collecting the formed suspension spheres, which are the dermal dermal papilla cell spheres. The dermal dermal papilla cell spheres and vesicles provided by the present application have better therapeutic effects on scarless healing of a wound surface.
Owner:FOURTH MILITARY MEDICAL UNIVERSITY

Umbilical cord mesenchymal stem cell membrane for treating alopecia and preparation method and application thereof

PendingCN122357439AMetaboliteCell membrane
This invention discloses an umbilical cord mesenchymal stem cell membrane for treating hair loss, its preparation method, and its application, belonging to the field of biomedical technology. The preparation method of the umbilical cord mesenchymal stem cell membrane includes the following steps: obtaining human umbilical cord mesenchymal stem cells, digesting, centrifuging, washing, and resuspending them to obtain a resuspended cell solution; subjecting the resuspended cell solution to repeated freeze-thaw cycles to break the cells, obtaining a cell lysate; and subjecting the cell lysate to differential centrifugation to obtain the umbilical cord mesenchymal stem cell membrane. This umbilical cord mesenchymal stem cell membrane retains the natural cell membrane structure and functional proteins, has a negatively charged surface, and exhibits good dispersibility and stability. It can improve the survival rate of dermal papilla cells without introducing live cells and effectively regulate the skin microecology and metabolite balance, demonstrating high safety, good biocompatibility, and excellent hair growth promotion effects.
Owner:JILIN UNIVERSITY

The method of hair transplant using dermal papilla and dermal sheath obtained from hair follicle

The method of hair transplant using dermal papilla and dermal sheath obtained from hair follicle as disclosed comprises the steps of: providing initial keratinocytes to be used for creating condition medium; creating and collecting condition medium from said keratinocytes; collecting hair follicle cells containing dermal papilla and dermal sheath from patients; culturing said cells as obtained from patients using condition medium collected from the prior step; injecting cultured cells in the predetermined amount and the predetermined ratio of dermal papilla cells and dermal sheath cells in the predetermined amount area characterized in that the step of injecting and collecting condition medium from said keratinocytes comprises the step of applying a solution obtained from the mixture of defined keratinocyte-serum free medium (DK-SFM) and Mesenchymal stem cell culture medium (MSC) to said initial keratinocytes, removing defined keratinocyte-serum free medium (DK-SFM), and extracting a condition medium from the said mixture and wherein the step of culturing hair follicle cells as obtained from patients comprises the step of applying condition medium extracted and MSC in the ratio of 1:1.
Owner:MEDEZE TREASURY PTE LTD

A non-removable hair growth composition and its use in hair growth products

PendingCN122163756ACosmetic preparationsHair cosmeticsAlopecia treatmentTyrosine
This invention relates to the field of hair growth composition technology, specifically to an anti-hair loss and hair growth composition and its application in hair growth products. The active ingredients of the composition include GHK-Gu and Erigeron tiglium extract, and preferably also include Eclipta prostrata extract. Through in-depth research, this invention has for the first time discovered that GHK-Gu and Erigeron tiglium extract can produce a synergistic effect at a specific ratio. Based on this, a ternary compound system containing Eclipta prostrata extract was optimized. The composition significantly improves hair density, diameter, and color through multi-target synergistic effects, including inhibiting 5α-reductase activity, promoting dermal papilla cell proliferation, and activating tyrosinase. This invention solves the technical problems of significant side effects from existing chemical drugs and complex compositions of traditional Chinese medicine compound formulas, providing a highly efficient, safe, and simplified solution for hair loss treatment. In vitro and in vivo experiments have confirmed its significant anti-hair loss, hair growth, and hair darkening effects.
Owner:GUANGZHOU YILENA BIOMEDICAL TECHNOLOGY CO LTD

Skin-like organ containing hair follicle structure and preparation method thereof

The invention discloses a skin organoid containing a hair follicle structure and a preparation method thereof, and belongs to the field of tissue engineering, the method comprises the following steps: separating hair follicle stem cells from dermal papilla cells; hair follicle induced aggregate is formed through synergistic induction of a Wnt activator, a BMP inhibitor and FGF; embedding the aggregate into a biphase gradient collagen-hyaluronic acid hydrogel to construct a corium layer; after keratinocytes are inoculated, hair follicles are activated and promoted to mature through a gas-liquid interface staged signal channel, and the obtained organoid contains complete epidermal layer, corium layer and hair follicle-like structures and can be used for hair research and drug screening.
Owner:SHENYANG ZEER TESTING SERVICE CO LTD

Peptides that promote hair growth and inhibit hair loss and their uses

ActiveCN120019061BMedicineDermal papillae
This invention relates to peptides with activity that promotes hair growth or prevents or inhibits hair loss. The peptides of this invention promote the proliferation of follicular dermal papilla cells (HFDPC) and human piloerection sheath cells (HHORSC), upregulate the expression of growth factors in HFDPC, and downregulate the expression level of Dickkopf-1 (DKK-1), a hair loss protein.
Owner:CAREGEN

Peptides that promote hair growth and inhibit hair loss and their uses

ActiveCN120019063BMedicineDermal papillae
This invention relates to peptides that promote hair growth or prevent or inhibit hair loss. The peptides of this invention promote the proliferation of follicular dermal papilla cells (HFDPC) and human pilosebaceous dermal sheath cells (HHORSC), upregulate the expression of β-catenin secondary factor in HFDPC, upregulate the expression of growth factors in HFDPC, and downregulate the expression level of Dickkopf-1 (DKK-1), a protein that causes hair loss.
Owner:CAREGEN

Compound composition for preventing hair loss and promoting hair growth and application thereof

The invention relates to the technical field of medicines, in particular to a compound composition for preventing alopecia and promoting hair growth and application thereof. The invention provides a compound composition for preventing hair loss and promoting hair growth. The compound composition is prepared from the following raw materials in parts by weight: 1-5 parts of amentoflavone and 1-10 parts of unodil. The invention provides an application of amentoflavone and unodil in hair loss prevention and hair growth. An in-vitro cell experiment shows that after the amentoflavone and the unodil are combined, the proliferation of human primary dermal papilla cells can be promoted. A preclinical animal experiment shows that after the amentoflavone and the unodil are locally used, the hair growth speed is increased, and the hair density is increased.
Owner:SHANGHAI DERMATOLOGY HOSPITAL

Anti-hair loss shampoo and preparation method thereof

The application discloses a hair loss prevention shampoo targeting CD133 positive hair follicle dermal papilla cells and a preparation method thereof. The shampoo comprises a hair follicle targeting single-domain antibody-active peptide fusion, a liposome-encapsulated compound plant extract, a biological surfactant, a protein stability surfactant and an auxiliary material. The fusion is formed by fusing an anti-CD133 VHH single-domain antibody and a 5alpha-reductase inhibiting peptide DK-HP-7 through a flexible connecting peptide. Based on the discovery that CD133 positive dermal papilla cells are a key cell group driving hair follicle formation, the application uses single-domain antibodies to achieve precise targeting of active ingredients. The DK-HP-7 designed through molecular docking has an IC 50 of 4.28 μM. The rhamnolipid and Kolliphor HS 15 complex system can make the fusion protein activity retention rate higher than 89% after being stored at 40 ℃ for 3 months. The shampoo can significantly antagonize dihydrotestosterone-induced hair follicle growth inhibition, promote hair follicles to enter the growth phase and increase hair follicle density, and has good skin safety and stability.
Owner:GUANGZHOU TAIYUAN BIOTECHNOLOGY CO LTD

Regulatory protein for activating hair papilla melanin to promote hair blackening and preparation method thereof

This invention discloses a regulatory protein that activates melanin in the dermal papilla to promote hair darkening and its preparation method, belonging to the field of biotechnology. The method includes: identifying key endogenous proteins that positively regulate melanin synthesis in dermal papilla cells through multi-omics analysis; constructing a humanized recombinant regulatory protein fused with a membrane-penetrating peptide; loading its coding sequence into a eukaryotic expression vector to achieve local and sustained expression; verifying its melanin-promoting efficacy using a three-dimensional hair follicle organoid model and animal experiments; and formulating a thermosensitive gel for topical application to improve hair follicle-targeted delivery efficiency. This invention aims to address the problems of unclear mechanisms of action and limited efficacy in existing hair-darkening products. It can directly activate the MITF signaling pathway, significantly promote the expression of key enzymes such as TYR and TRP-1, induce melanosome synthesis and deposition, resulting in a hair color darkening rate of over 95%, with long-lasting efficacy and good safety.
Owner:GUANGZHOU TONGXI BIOLOGICAL RESEARCH CO LTD

Peptides that promote hair growth and inhibit hair loss and their uses

ActiveCN120019062BDermal papillaeCatenin Proteins
This invention relates to peptides with activity that promotes hair growth or prevents or inhibits hair loss. The peptides of this invention promote the proliferation of human follicular dermal papilla cells (HFDPC), activate cell proliferation-related signaling proteins and β-catenin in HFDPC, enhance the expression levels of downstream genes of β-catenin, downregulate the expression level of the hair loss protein DKK-1, increase the expression of keratin in hair follicle outer root sheath cells (HORSC), and upregulate the expression of transcription factors involved in cell activation in germinal stromal cells (GMC).
Owner:CAREGEN

Peptides having activity to promote hair growth and inhibit hair loss and uses thereof

ActiveCN120035599BCosmetic preparationsHair cosmeticsMedicineHair root sheath
The present invention relates to peptides having an activity of promoting hair growth or preventing or inhibiting hair loss. The peptides according to the present invention promote proliferation of hair follicle dermal papilla cells (HFDPC) and human hair root sheath cells (HHORSC), upregulate expression of a beta-catenin secondary factor in hair follicle dermal papilla cells (HFDPC), and upregulate expression of a growth factor.
Owner:CAREGEN

Composition for promoting collagen production comprising amidated amino acid

The present invention relates to a composition for promoting collagen production comprising amidated amino acids, and more specifically, to a composition that promotes collagen production in various cells such as fibroblasts, follicular cells, dermal papilla cells, endothelial cells, chondrocytes, muscle cells, and osteoblasts, thereby preventing, improving, or treating various diseases and lesions that may result from collagen reduction in the skin, hair, blood vessels, cartilage, tendons, connective tissues, muscles, bones, teeth, and periodontal tissues.
Owner:KYUNGPOOK NAT UNIV IND ACADEMIC COOP FOUND

A plant lactobacillus plantarum ccfm1351 postbiotic for preventing hair loss and protecting hair based on multi-target

The application discloses a plant lactobacillus plantarum CCFM1351 prepared metaplasma based on multi-target for preventing hair loss and protecting hair, and belongs to the technical field of microorganisms and the technical field of medicine. The plant lactobacillus plantarum CCFM1351 prepared metaplasma has the functions of regulating hair follicle development and inhibiting apoptosis, can inhibit the expression of BAX, TGF-beta 2 and DKK-1 of hair follicle dermal papilla cells (HDPC), and promote the expression of VEGF in HDPC. Compared with the same concentration of Platycladus orientalis, the Platycladus orientalis fermentation supernatant prepared by the plant lactobacillus plantarum CCFM1351 can promote the expression of Cyclin D1 mRNA and IGF-1 mRNA of HDPC. The plant lactobacillus plantarum CCFM1351 prepared metaplasma can be used for preparing products for preventing and / or treating hair loss, and has great application prospect.
Owner:JIANGNAN UNIV

A defined serum-free induction medium and induction method and application for promoting differentiation of adipose mesenchymal cells into dermal papilla cells

The present application relates to the technical field of regenerative medicine application, and particularly relates to a serum-free induction medium with clear components for promoting differentiation of adipose-derived mesenchymal cells into dermal papilla cells, and an induction method and application. A method for promoting differentiation of adipose-derived mesenchymal cells into dermal papilla cells, which is to use a serum-free induction medium to induce culture of adipose-derived mesenchymal cells, so that the adipose-derived mesenchymal cells are differentiated into dermal papilla cells. The formula of the serum-free induction medium comprises hepatocyte growth factor, hydrocortisone, bone morphogenetic protein 2, BIO, and basic fibroblast growth factor. The present application is based on the differentiation potential of adipose-derived mesenchymal cells and the mechanism of maintaining the function of dermal papilla cells, and constructs a serum-free induction system with clear components to realize directional differentiation. The technical scheme can solve the technical problem that there is no method for inducing directional differentiation of adipose-derived mesenchymal cells into dermal papilla cells with effective hair follicle regulation function in the prior art, and has ideal application and popularization potential.
Owner:CHENGDU YUNCE MEDICAL BIOTECHNOLOGY CO LTD

Methods for preparing dermal papilla cell spheroids and their applications

PendingCN122303133ATissue repairCyclodextrin
This invention provides a method for preparing dermal papilla cell spheres, comprising mixing hydrophobic hyaluronic acid grafted with hydrophobic groups and cyclodextrin modified with crosslinking groups to obtain a hydrogel prepolymer, adding a dermal papilla cell suspension to the hydrogel prepolymer to initiate a crosslinking reaction of the crosslinking groups to form a dynamically crosslinked hydrogel loaded with cells, and then adding a cell culture medium. The hydrophobic groups are selected from tert-butylbenzene, ibuprofen, menthol, etc. This method can rapidly obtain dermal papilla cell spheres and maintain the stemness of dermal papilla cells and the induction ability of hair follicle tissue, which can be used to achieve hair follicle tissue repair and regeneration, and promote hair growth. This invention also relates to the application of the method for preparing dermal papilla cell spheres in the preparation of compositions that promote hair follicle tissue repair, regeneration, or hair growth, wherein the composition includes a dynamically crosslinked hydrogel loaded with dermal papilla cells, which can be used to prevent and treat hair loss.
Owner:SOUTH CHINA UNIV OF TECH

Immortalized cell line of human dermal papilla cells as well as construction method and application of immortalized cell line

The invention relates to an immortalized cell line of human dermal papilla cells as well as a construction method and application of the immortalized cell line. The human dermal papilla cells are named as DYZ-20, the immortalized cell line is preserved in the China Center for Type Culture Collection on July 2, 2025, survival is detected, and the preservation number is CCTCC NO: C2025204. The immortalized cell line of the human dermal papilla cells can normally express alkaline phosphatase and a dermal papilla characteristic gene, and hair can grow after the immortalized cell line is transplanted into mouse skin.
Owner:TSINGHUA SHENZHEN INTERNATIONAL GRADUATE SCHOOL

Hair follicle-derived staphylococcus epidermidis CCSM013 with hair loss prevention and hair care effects and metagen thereof

The invention discloses staphylococcus epidermidis CCSM013 with anti-hair loss and hair care effects and a metagen of the staphylococcus epidermidis CCSM013, and belongs to the technical field of microorganisms and the technical field of medicines. The staphylococcus epidermidis CCSM013 and the metagen thereof provided by the invention have the effects of preventing hair loss and protecting hair, and the effects comprise the following steps: promoting the expression of beta-catenin in follicle dermal papilla cells; the content of FGF-7 in follicle dermal papilla cells is increased; the mouse hair growth is promoted; the content of Wnt10b in mouse skin tissues is up-regulated; the content of beta-catenin in mouse skin tissues is up-regulated; the content of VEGF in mouse skin tissue is up-regulated; and the content of TGF-beta1 in mouse skin tissues is reduced. The staphylococcus epidermidis CCSM013 and the metagen thereof provided by the invention can be used for preparing medicines or cosmetics for preventing and / or treating alopecia, and have a wide application prospect.
Owner:JIANGNAN UNIV

Peptide having activity of promoting hair growth and inhibiting alopecia and use thereof

PendingCN121866264ACosmetic preparationsHair cosmeticsDermal papillaeCatenin Proteins
The invention relates to a peptide capable of promoting hair growth and preventing and inhibiting alopecia and application thereof. The peptide provided by the invention has the following activities: promoting proliferation of hair follicle dermal papilla cells (HFDPC), activating cell proliferation-related signaling proteins and beta-catenin in human dermal papilla cells, enhancing the expression level of growth factor-related genes, enhancing the expression level of beta-catenin downstream genes, reducing the expression level of alopecia protein DKK-1, and promoting the growth of the hair follicle dermal papilla cells. Increasing the expression of keratin in hair outer root sheath cells (HORSC), and increasing the expression of transcription factors involved in cell activation in hair growth stromal cells (GMC); and promoting differentiation of hair follicle stem cells (HFSC).
Owner:CAREGEN

Hair follicle epithelial stem cell preparation and hair follicle primordium preparation

Epithelial stem cells (15), such as hair follicle epithelial stem cells, are cultured on a scaffold (13) comprising a molecule containing an extracellular cadherin (cadherin EC) domain. The epithelial stem cells 15 are then separated from the scaffold 13. The epithelial stem cells 15 are prepared through the abovementioned process. Thereafter, a hair follicle primordium10 may be produced by three-dimensionally culturing the epithelial stem cells 15 and mesenchymal cells 16 in a state in which a layer of the epithelial stem cells 15 and a layer of the mesenchymal cells 16 overlap. The mesenchymal cell 16 layer may include a layer of hair papilla cells and a layer of cells constituted of dermal pluripotent cells (DDMCs) or dermal sheath cells (DS cells). The epithelial stem cell 15 layer may overlap the hair papilla cell-layer side of the mesenchymal cell 16 layer.
Owner:ORGANTECH INC

A defined serum-free induction medium and induction method for promoting differentiation of mesenchymal stem cells into dermal papilla cells

The present application relates to the technical field of stem cells, and particularly relates to a serum-free induction medium with clear components for promoting differentiation of induced mesenchymal stem cells into dermal papilla cells and an induction method. A small molecule compound composition comprises all-trans retinoic acid, forskolin, CHIR-99021 and minoxidil in a molar ratio of 0.2-5:2-50:0.2-10:0.2-10. The present application adopts multiple small molecule compounds to promote differentiation and function improvement of dermal papilla cells and transformation of mesenchymal stem cells into dermal papilla cells. The first three promote differentiation of dermal papilla cells, and the last one promotes proliferation and inhibits apoptosis of dermal papilla cells. The present application can solve the technical problems of high process cost and unsatisfactory clinical safety caused by the need to add cytokines in the process of inducing differentiation of stem cells into dermal papilla cells, and speeds up the induction process, and has an ideal application prospect.
Owner:CHENGDU YUNCE MEDICAL BIOTECHNOLOGY CO LTD