65 results about "Dermal papillae" patented technology

An object of the present invention is to provide a formulation effective for activation of hair dermal papilla cells. Provided are a composition for activation of hair dermal papilla cells, a composition for hair regrowth promotion or hair growth, a composition for hair quality improvement, and a composition for FGF-2, FGF-7 or VEGF production promotion, containing a culture supernatant and / or extracellular vesicles of a microalga.

The invention relates to the technical field of stem cells, in particular to a serum-free induction medium with definite components for promoting and inducing mesenchymal stem cells to be differentiated into dermal papilla cells and an induction method. The small molecule compound composition is prepared from all-transretinoic acid, calomel, CHIR-99021 and minoxidil according to the molar ratio of (0.2 to 5): (2 to 50): (0.2 to 10): (0.2 to 10). According to the scheme, a plurality of small molecule compounds are adopted and have a synergistic effect, so that differentiation and function improvement of dermal papilla cells are promoted, and transformation of mesenchymal stem cells to the dermal papilla cells is promoted. The former three promote dermal papilla cell differentiation, and the latter promotes dermal papilla cell proliferation and inhibits apoptosis. According to the technical scheme, the technical problems that cell factors need to be added in the process of forming dermal papilla cells from stem cells through induced differentiation, so that the process cost is high, and the clinical safety is not ideal can be solved, the induction process is accelerated, and the method has ideal popularization and application prospects.

The invention relates to the technical field of daily cosmetics, in particular to a peony extract with scalp anti-aging and anti-hair-loss functions and application. The peony extract disclosed by the invention is a peony flower extract, the mass fraction of peony flower peptide in the peony flower extract is greater than or equal to 10%, and the peony extract disclosed by the invention is found to have the effects of improving the activity of superoxide dismutase (SOD) on human hair papilla cells for the first time; the positive cell rate of beta-galactosidase (SA-beta-Gal) of human hair papilla cells is inhibited, and the functions of senescent cells are reduced; meanwhile, the functions of promoting hair follicles to express growth factors and promoting hair growth are achieved. The method can be applied to preparation of scalp care products, and the anti-aging or anti-hair-loss function of scalp is achieved.

The present invention relates to a peptide having an activity of promoting hair growth or preventing or inhibiting hair loss. The peptide of the present invention has an activity of promoting proliferation of hair follicle dermal papilla cells (HFDPC), activating a cell proliferation-related signal protein in human dermal papilla cells, activating β-catenin, improving expression levels of factors downstream of β-catenin, down-regulating expression levels of hair-loss protein DKK-1, and increasing expression of keratins in hair outer root sheath cells (HORSC).

The application discloses a plant Lactobacillus plantarum CCFM1353, a metapolybiose prepared from the plant Lactobacillus plantarum CCFM1353 for preventing hair loss and promoting hair growth, and application of the metapolybiose in combination with transformed Chinese arborvitae leaves, and belongs to the technical fields of microorganisms and medicines. The plant Lactobacillus plantarum CCFM1353 and the metapolybiose and symbiotic preparation prepared from the plant Lactobacillus plantarum CCFM1353 can promote the expression of IGF-1, VEGF and TGF-beta 2 of hair follicle dermal papilla cells (HDPC), promote hair growth of mice, and promote the expression of VEGF, IGF-1, Wnt10b, Cyclin D1, Ki67 and Bcl2 in the skin of mice. The plant Lactobacillus plantarum CCFM1353 and the metapolybiose and symbiotic preparation prepared from the plant Lactobacillus plantarum CCFM1353 can be used for preparing products for preventing and / or treating hair loss, and have great application prospects.

The present invention relates to a peptide with the activity of promoting hair growth or preventing or inhibiting alopecia. The peptide of the present invention promotes the proliferation of human follicular dermal papilla cells (HFDPC), activates cell proliferation-related signaling proteins and beta-catenin in human dermal papilla cells, enhances the expression levels of beta-catenin downstream genes, down-regulates the expression levels of the hair loss protein DKK-1, increases the expression of keratin proteins in hair outer root sheath cells (HORSC) and up-regulates the expression of transcription factors involved in cell activation in germinal matrix cells (GMC).

The invention belongs to the technical field of biological medicine, and particularly discloses a small molecule compound SR4370 for promoting hair follicle growth and regeneration and application thereof. High-throughput screening finds that SR4370 can induce dermal fibroblasts to be reprogrammed into DP-like cells with characteristics of hair papilla cells and up-regulate ALP, WNT5A and other marker genes, and in-vivo and in-vitro experiments prove that SR4370 can activate resting hair follicles, promote in-vitro hair follicle growth and enhance the regeneration capacity of dermal stem cells. The invention provides a safe and efficient hair follicle regeneration strategy, and provides a new tool for alopecia treatment and tissue engineering.

This invention relates to a composition for promoting hair growth, comprising a dynamically cross-linked hydrogel loaded with dermal papilla cells. The dynamically cross-linked hydrogel includes adamantane-grafted hyaluronic acid, gelatin, and cyclodextrin modified with cross-linking groups. A three-dimensional network of the dynamic hydrogel is formed through host-guest interactions and the cross-linking of the cross-linking groups. The invention also provides a method for preparing the composition for promoting hair growth, comprising mixing adamantane-grafted hyaluronic acid and cyclodextrin modified with cross-linking groups to obtain a hydrogel prepolymer; adding a dermal papilla cell suspension to the hydrogel prepolymer to initiate a cross-linking reaction of the cross-linking groups to form a dynamically cross-linked hydrogel loaded with dermal papilla cells. The composition can be directly delivered to the depth of hair follicles in the scalp via mesotherapy injection to activate dormant hair follicles from the root, promote new hair growth, and thicken existing hair. The hair regeneration effect is rapid and long-lasting.

The invention provides a hair growth composition or a pharmaceutical composition containing rutin and application of rutin in preparation of the hair growth composition or the pharmaceutical composition. The invention provides application of rutin in improvement of hair growth and prevention of alopecia. The rutin can improve hair growth and prevent alopecia by activating a Wnt / beta-catenin signal channel, promoting proliferation of human dermal papilla cells and promoting growth of hair follicles.

The present invention relates to a composition for preventing hair loss or promoting hair growth containing a milk thistle flower extract as an active ingredient. More specifically, the present invention relates to a cosmetic composition, pharmaceutical composition and food composition containing a milk thistle flower extract as an active ingredient, which are able to promote hair growth by inducing the proliferation of dermal papilla cells reduced due to senescence, and activate dermal papilla cells by inhibiting the senescence of dermal papilla cells through inhibition of the activity of senescence-associated β-galactosidase (SA-β-Gal) and the production of senescence-associated secretory phenotypes, thereby preventing hair loss or promoting hair.The composition according to the present invention has no skin irritation and cytotoxicity, and thus has excellent safety for the human body, and at the same time, is very effective in promoting the proliferation of dermal papilla cells and inhibiting the senescence of dermal papilla cells. Thus, it may be useful as an active ingredient in a cosmetic composition, a pharmaceutical composition or a food composition for preventing hair loss or promoting hair growth.

The invention relates to the technical field of regenerative medicine application, in particular to a serum-free induction culture medium with definite components for promoting adipose tissue-derived cells to be differentiated into dermal papilla cells, an induction method and application. The invention relates to a method for promoting adipose tissue-derived cells to differentiate into dermal papilla cells, which is characterized in that the adipose tissue-derived cells are induced and cultured by using a serum-free induction culture medium, so that the adipose tissue-derived cells are differentiated into the dermal papilla cells. The formula of the serum-free induction culture medium comprises hepatocyte growth factors, hydrocortisone, bone morphogenetic protein 2, BIO and basic fibroblast growth factors. Based on the differentiation potential of adipose tissue-derived mesenchymal cells and the function maintenance mechanism of dermal papilla cells, a serum-free induction system with definite components is constructed, and directional differentiation is realized. According to the technical scheme, the technical problem that a method for inducing the adipose-derived mesenchymal cells to be directionally differentiated into the dermal papilla cells with the effective hair follicle regulation function is lacked in the prior art can be solved, and the method has ideal application and popularization potential.

This invention discloses the use of polylactic acid (PLA) and its copolymers in the preparation of products that promote hair follicle growth and repair, belonging to the field of biomedical technology. Through in vitro and in vivo experiments, this invention demonstrates that PLA can significantly upregulate the expression of lactate dehydrogenase A and transforming growth factor β2 in dermal papilla cells, promoting dermal papilla cell proliferation and hair follicle growth and damage repair. Based on these findings, this invention provides the application of PLA and its copolymers in the preparation of products that promote hair follicle growth and damage repair, offering a safe and effective treatment strategy for hair follicle-related diseases.

The invention discloses an organic acid-containing anti-hair-loss composition based on a cacumen biotae extract, which comprises the cacumen biotae extract and an organic acid as anti-hair-loss active components, and the acid is succinic acid or malic acid or a combination of the succinic acid and the malic acid. The invention also provides application of the anti-hair loss composition in regulating hair follicle energy metabolism and preventing hair loss. The composition containing the cacumen biotae extract, the succinic acid and the malic acid can promote the activity of hair papilla cells, enhance the energy metabolism of the cells, recover the function of hair follicles damaged by oxidation and recover the energy metabolism of the hair follicles damaged by oxidation, so that the composition has a good effect of preventing and treating alopecia, stimulates hair growth and has few toxic and side effects.

The invention provides a traditional Chinese medicine composition for preventing hair loss and promoting hair growth and application thereof, and relates to the technical field of hair loss prevention and promoting hair growth, the traditional Chinese medicine composition is composed of Chinese doughnut, cockscomb, albizia flower, Chinese honey locust, schizonepeta, lithospermum, polygonum multiflorum, prepared rehmannia root, notopterygium root, radix angelicae pubescentis, ruddle and periostracum cicada; in-vitro experiments show that the traditional Chinese medicine composition can promote proliferation of human dermal papilla cells HDPC, relieve the damage degree of HDPC and induce cycle transformation of hair follicles, so that the effect of promoting hair growth is achieved; animal experiments show that the traditional Chinese medicine composition can significantly increase the number of hair follicles and the thickness of skin and dermis fat of mice, reduce the levels of mouse serum IL-2, TNF-alpha and TGF-beta1 and reduce the levels of testosterone, dihydrotestosterone and estradiol in mouse skin tissue, so that hair follicle fibrosis is inhibited, the growth period of hair follicles is accelerated, the growth period is prolonged, the hair loss phenomenon is improved, and the hair loss rate is increased. The hair growth is promoted.

An artificial hair follicle germ 10 has a three-dimensional structure in which a first block 11 including hair follicle epithelial stem cells, a second block 12 including hair papilla cells, and a third block 13 including dermal-derived mesenchymal cells (DDMCs) are stacked in this order. A method for artificially preparing a hair follicle germ 10 includes three-dimensionally culturing a first block 11 including hair follicle epithelial stem cells, a second block 12 including hair papilla cells, and a third block 13 including dermal-derived mesenchymal cells (DDMCs), these blocks being stacked in this order.

The present application belongs to the field of biological materials and regenerative medicine, and particularly relates to a dermal dermal papilla cell sphere, an in-vitro culture method thereof, vesicles and uses. The dermal dermal papilla cell sphere is prepared according to the following method: extracting primary dermal dermal papilla cells from an in-vitro dermal dermal papilla tissue, subculturing for 3-5 generations; inoculating the subcultured dermal dermal papilla cells into an SLF-3D culture system for culture, collecting semi-suspended spheres when the dermal dermal papilla cells grow to a fusion degree of 90-100%, separating the spheres into single cells, and re-inoculating the single cells into the original SLF-3D culture system for culture for 3-5 days, and collecting the formed suspension spheres, which are the dermal dermal papilla cell spheres. The dermal dermal papilla cell spheres and vesicles provided by the present application have better therapeutic effects on scarless healing of a wound surface.

This invention discloses an umbilical cord mesenchymal stem cell membrane for treating hair loss, its preparation method, and its application, belonging to the field of biomedical technology. The preparation method of the umbilical cord mesenchymal stem cell membrane includes the following steps: obtaining human umbilical cord mesenchymal stem cells, digesting, centrifuging, washing, and resuspending them to obtain a resuspended cell solution; subjecting the resuspended cell solution to repeated freeze-thaw cycles to break the cells, obtaining a cell lysate; and subjecting the cell lysate to differential centrifugation to obtain the umbilical cord mesenchymal stem cell membrane. This umbilical cord mesenchymal stem cell membrane retains the natural cell membrane structure and functional proteins, has a negatively charged surface, and exhibits good dispersibility and stability. It can improve the survival rate of dermal papilla cells without introducing live cells and effectively regulate the skin microecology and metabolite balance, demonstrating high safety, good biocompatibility, and excellent hair growth promotion effects.

The method of hair transplant using dermal papilla and dermal sheath obtained from hair follicle as disclosed comprises the steps of: providing initial keratinocytes to be used for creating condition medium; creating and collecting condition medium from said keratinocytes; collecting hair follicle cells containing dermal papilla and dermal sheath from patients; culturing said cells as obtained from patients using condition medium collected from the prior step; injecting cultured cells in the predetermined amount and the predetermined ratio of dermal papilla cells and dermal sheath cells in the predetermined amount area characterized in that the step of injecting and collecting condition medium from said keratinocytes comprises the step of applying a solution obtained from the mixture of defined keratinocyte-serum free medium (DK-SFM) and Mesenchymal stem cell culture medium (MSC) to said initial keratinocytes, removing defined keratinocyte-serum free medium (DK-SFM), and extracting a condition medium from the said mixture and wherein the step of culturing hair follicle cells as obtained from patients comprises the step of applying condition medium extracted and MSC in the ratio of 1:1.

This invention relates to the field of hair growth composition technology, specifically to an anti-hair loss and hair growth composition and its application in hair growth products. The active ingredients of the composition include GHK-Gu and Erigeron tiglium extract, and preferably also include Eclipta prostrata extract. Through in-depth research, this invention has for the first time discovered that GHK-Gu and Erigeron tiglium extract can produce a synergistic effect at a specific ratio. Based on this, a ternary compound system containing Eclipta prostrata extract was optimized. The composition significantly improves hair density, diameter, and color through multi-target synergistic effects, including inhibiting 5α-reductase activity, promoting dermal papilla cell proliferation, and activating tyrosinase. This invention solves the technical problems of significant side effects from existing chemical drugs and complex compositions of traditional Chinese medicine compound formulas, providing a highly efficient, safe, and simplified solution for hair loss treatment. In vitro and in vivo experiments have confirmed its significant anti-hair loss, hair growth, and hair darkening effects.

The invention discloses a skin organoid containing a hair follicle structure and a preparation method thereof, and belongs to the field of tissue engineering, the method comprises the following steps: separating hair follicle stem cells from dermal papilla cells; hair follicle induced aggregate is formed through synergistic induction of a Wnt activator, a BMP inhibitor and FGF; embedding the aggregate into a biphase gradient collagen-hyaluronic acid hydrogel to construct a corium layer; after keratinocytes are inoculated, hair follicles are activated and promoted to mature through a gas-liquid interface staged signal channel, and the obtained organoid contains complete epidermal layer, corium layer and hair follicle-like structures and can be used for hair research and drug screening.

This invention relates to peptides with activity that promotes hair growth or prevents or inhibits hair loss. The peptides of this invention promote the proliferation of follicular dermal papilla cells (HFDPC) and human piloerection sheath cells (HHORSC), upregulate the expression of growth factors in HFDPC, and downregulate the expression level of Dickkopf-1 (DKK-1), a hair loss protein.

This invention relates to peptides that promote hair growth or prevent or inhibit hair loss. The peptides of this invention promote the proliferation of follicular dermal papilla cells (HFDPC) and human pilosebaceous dermal sheath cells (HHORSC), upregulate the expression of β-catenin secondary factor in HFDPC, upregulate the expression of growth factors in HFDPC, and downregulate the expression level of Dickkopf-1 (DKK-1), a protein that causes hair loss.

The invention relates to the technical field of medicines, in particular to a compound composition for preventing alopecia and promoting hair growth and application thereof. The invention provides a compound composition for preventing hair loss and promoting hair growth. The compound composition is prepared from the following raw materials in parts by weight: 1-5 parts of amentoflavone and 1-10 parts of unodil. The invention provides an application of amentoflavone and unodil in hair loss prevention and hair growth. An in-vitro cell experiment shows that after the amentoflavone and the unodil are combined, the proliferation of human primary dermal papilla cells can be promoted. A preclinical animal experiment shows that after the amentoflavone and the unodil are locally used, the hair growth speed is increased, and the hair density is increased.

The application discloses a hair loss prevention shampoo targeting CD133 positive hair follicle dermal papilla cells and a preparation method thereof. The shampoo comprises a hair follicle targeting single-domain antibody-active peptide fusion, a liposome-encapsulated compound plant extract, a biological surfactant, a protein stability surfactant and an auxiliary material. The fusion is formed by fusing an anti-CD133 VHH single-domain antibody and a 5alpha-reductase inhibiting peptide DK-HP-7 through a flexible connecting peptide. Based on the discovery that CD133 positive dermal papilla cells are a key cell group driving hair follicle formation, the application uses single-domain antibodies to achieve precise targeting of active ingredients. The DK-HP-7 designed through molecular docking has an IC 50 of 4.28 μM. The rhamnolipid and Kolliphor HS 15 complex system can make the fusion protein activity retention rate higher than 89% after being stored at 40 ℃ for 3 months. The shampoo can significantly antagonize dihydrotestosterone-induced hair follicle growth inhibition, promote hair follicles to enter the growth phase and increase hair follicle density, and has good skin safety and stability.

This invention discloses a regulatory protein that activates melanin in the dermal papilla to promote hair darkening and its preparation method, belonging to the field of biotechnology. The method includes: identifying key endogenous proteins that positively regulate melanin synthesis in dermal papilla cells through multi-omics analysis; constructing a humanized recombinant regulatory protein fused with a membrane-penetrating peptide; loading its coding sequence into a eukaryotic expression vector to achieve local and sustained expression; verifying its melanin-promoting efficacy using a three-dimensional hair follicle organoid model and animal experiments; and formulating a thermosensitive gel for topical application to improve hair follicle-targeted delivery efficiency. This invention aims to address the problems of unclear mechanisms of action and limited efficacy in existing hair-darkening products. It can directly activate the MITF signaling pathway, significantly promote the expression of key enzymes such as TYR and TRP-1, induce melanosome synthesis and deposition, resulting in a hair color darkening rate of over 95%, with long-lasting efficacy and good safety.

This invention relates to peptides with activity that promotes hair growth or prevents or inhibits hair loss. The peptides of this invention promote the proliferation of human follicular dermal papilla cells (HFDPC), activate cell proliferation-related signaling proteins and β-catenin in HFDPC, enhance the expression levels of downstream genes of β-catenin, downregulate the expression level of the hair loss protein DKK-1, increase the expression of keratin in hair follicle outer root sheath cells (HORSC), and upregulate the expression of transcription factors involved in cell activation in germinal stromal cells (GMC).

The present invention relates to peptides having an activity of promoting hair growth or preventing or inhibiting hair loss. The peptides according to the present invention promote proliferation of hair follicle dermal papilla cells (HFDPC) and human hair root sheath cells (HHORSC), upregulate expression of a beta-catenin secondary factor in hair follicle dermal papilla cells (HFDPC), and upregulate expression of a growth factor.

The present invention relates to a composition for promoting collagen production comprising amidated amino acids, and more specifically, to a composition that promotes collagen production in various cells such as fibroblasts, follicular cells, dermal papilla cells, endothelial cells, chondrocytes, muscle cells, and osteoblasts, thereby preventing, improving, or treating various diseases and lesions that may result from collagen reduction in the skin, hair, blood vessels, cartilage, tendons, connective tissues, muscles, bones, teeth, and periodontal tissues.

The application discloses a plant lactobacillus plantarum CCFM1351 prepared metaplasma based on multi-target for preventing hair loss and protecting hair, and belongs to the technical field of microorganisms and the technical field of medicine. The plant lactobacillus plantarum CCFM1351 prepared metaplasma has the functions of regulating hair follicle development and inhibiting apoptosis, can inhibit the expression of BAX, TGF-beta 2 and DKK-1 of hair follicle dermal papilla cells (HDPC), and promote the expression of VEGF in HDPC. Compared with the same concentration of Platycladus orientalis, the Platycladus orientalis fermentation supernatant prepared by the plant lactobacillus plantarum CCFM1351 can promote the expression of Cyclin D1 mRNA and IGF-1 mRNA of HDPC. The plant lactobacillus plantarum CCFM1351 prepared metaplasma can be used for preparing products for preventing and / or treating hair loss, and has great application prospect.

The invention relates to the technical field of alopecia treatment and regenerative medicine, in particular to application of embryonic stem cell exosomes (ESC-Exos) in alopecia treatment. Specifically, ESC-Exos is introduced into a scalp dermis layer through a microneedle, and improvement of a hair follicle microenvironment and promotion of hair regeneration are realized by using various growth factors, micro-RNA and immunomodulatory factors carried by the ESC-Exos. According to the method for treating alopecia through the ESC-Exos, the penetrability and absorptivity of exosomes are improved through a microneedle technology, so that the ESC-Exos enter hair follicles and surrounding tissues of the hair follicles, the functions of dermal papilla cells are activated, angiogenesis is promoted, VEGF expression is up-regulated, a Wnt / beta-catenin signal channel is regulated and controlled, long-term hair growth is started, local microinflammation is relieved through an anti-inflammatory mechanism, and the effect of treating alopecia is achieved. Therefore, regeneration treatment of alopecia is realized. The method has the advantages of simplicity and convenience in operation, small trauma, high safety, remarkable curative effect and the like, and is suitable for auxiliary or alternative treatment of various types of male and female alopecia.