Application of hair follicle stem cell-derived exosome in promoting hair follicle stem cell proliferation and differentiation into hair follicle cells

A hair follicle stem cell and hair follicle cell technology, applied in the field of hair follicle stem cell-derived exosomes, can solve problems such as the function or related activity of exosomes that have not yet been derived from hair follicle stem cells

Active Publication Date: 2020-08-04
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] To date, there have been no reports on the function or related activities of hair follicle stem cell-derived exosomes

Method used

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  • Application of hair follicle stem cell-derived exosome in promoting hair follicle stem cell proliferation and differentiation into hair follicle cells
  • Application of hair follicle stem cell-derived exosome in promoting hair follicle stem cell proliferation and differentiation into hair follicle cells
  • Application of hair follicle stem cell-derived exosome in promoting hair follicle stem cell proliferation and differentiation into hair follicle cells

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0048] Experimental example 1 Effect of melatonin on hair follicle stem cell proliferation and its exosome secretion

[0049] 1 Experimental materials and reagents

[0050] 1.1 Experimental materials

[0051] An isolated and purified hair follicle stem cell line from Inner Mongolia cashmere goats.

[0052] 1.2 Experimental reagents

[0053] DMEM / F12, fetal bovine serum (FBS) were purchased from Hyclone; DMSO, MTT were purchased from Coolaber; epidermal growth factor (EGF), melatonin (Melatonin) were purchased from Sigma; Insulin-Transferrin-Selenium (ITS), 0.25% pancreatic Protease was purchased from Gibco; double antibody (PS) was purchased from Solebol; Total Exosome Isolation Reagent (from cellculture media) was purchased from Invitrogen; RIPA lysate and BCA Protein Assay Kit were purchased from Shanghai Sangong.

[0054] 2. Experimental method

[0055] 2.1 Thawing and recovery of hair follicle stem cells

[0056] (1) Take out P3 generation hair follicle stem cells (P4...

experiment example 2

[0098] Experimental example 2 Effect of melatonin on exosomes derived from hair follicle stem cells

[0099] 1 Experimental materials and reagents

[0100] 1.1 Experimental materials

[0101] The isolated and purified Inner Mongolia cashmere goat hair follicle stem cells provided by our laboratory.

[0102] 1.2 Experimental reagents

[0103] DMEM / F12 and fetal bovine serum (FBS) were purchased from Hyclone; epidermal growth factor (EGF), melatonin (Melatonin), Tris-base, and Glycine were purchased from Sigma; Luzindole and 4-P-PDOT were purchased from ChemCruz; Insulin -Transferrin-Selenium (ITS), 0.25% trypsin were purchased from Gibco; double antibody (PS), 5× protein loading buffer was purchased from Solebol; Total Exosome Isolation Reagent (from cell culture media) was purchased from Invitrogen; RIPA lysate, BCA Protein Assay Kit, SDS-PAGE Denaturing Acrylamide Gel Rapid Preparation Kit, SDS, skimmed milk powder were purchased from Shanghai Sangong; Mouse Anti-CD81 Anti...

experiment example 3

[0144] Experimental Example 3 Effect of Hair Follicle Stem Cell-Derived Exosomes on Recipient Cells

[0145] 1 Experimental materials and reagents

[0146] 1.1 Experimental materials

[0147] Inner Mongolia cashmere goat P5 hair follicle stem cells derived from exosomes, hair follicle stem cells, and hair papilla cells.

[0148] 1.2 Experimental reagents

[0149] DMEM / F12 and fetal bovine serum (FBS) were purchased from Hyclone; epidermal growth factor (EGF), melatonin (Melatonin), PKH67, and crystal violet were purchased from Sigma; Luzindole and 4-P-PDOT were purchased from ChemCruz; Insulin -Transferrin-Selenium (ITS), 0.25% trypsin were purchased from Gibco; double antibody (PS), 4% paraformaldehyde, Triton X100, DAPI were purchased from Suo Laibao; Total Exosome Isolation Reagent (from cell culturemedia) was purchased from Invitrogen; BCA Protein Assay Kit and BSA were purchased from Shanghai Sangong; RNAiso Plus, reverse transcription kit, PCR Taq, 2×SYBR Premix Ex Ta...

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Abstract

The invention discloses application of a hair follicle stem cell-derived exosome in promoting hair follicle stem cell proliferation and differentiation into hair follicle cells. It is found that the exosome derived from the hair follicle stem cells can enter recipient cells, and can promote proliferation of the hair follicle stem cells and dermal papillae cells, wherein the hair follicle stem cell-derived exosome stimulated by melatonin has a more obvious effect of promoting hair follicle stem cell proliferation. It is further found that the hair follicle stem cell-derived exosome can promotemigration of hair follicle stem cells and dermal papillae cells, and the migration promoting effect of the melatonin-stimulated hair follicle stem cell-derived exosome is more obvious. The hair follicle stem cell-derived exosome can promote the expression of hair follicle development-related genes of hair follicle stem cells and dermal papillae cells so as to promote the differentiation of the hair follicle stem cells into the hair follicle cells, and the melatonin-stimulated hair follicle stem cell-derived exosome has a more obvious effect of promoting the proliferation and differentiation ofthe hair follicle stem cells.

Description

technical field [0001] The present invention relates to a new use of exosomes derived from hair follicle stem cells, in particular to the use of exosomes derived from hair follicle stem cells in promoting the proliferation of hair follicle stem cells and the differentiation of hair follicle stem cells into hair follicle cells, and belongs to the field of new uses of exosomes derived from hair follicle stem cells. Background technique [0002] Goats (Capra hircus), also known as summer sheep, black sheep or goats, are one of the first domesticated domesticated animals, like sheep. Classified in Vertebrate Phylum, Mammalia, Artiodactyla, Bovidae, Ovis subfamily, Capra subfamily, Capra. Goat breeds are divided into three types according to their economic uses: milk type, meat type, and cashmere type. [0003] Cashmere is one of the finest and softest fibers produced by animals and is used exclusively in the production of luxury textile products. Most cashmere is produced in c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
CPCC12N5/0627C12N5/0628C12N2501/80
Inventor 高峰刘迎春陈钰萌彭耀
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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