Construction method for human hair follicle stem cell bank

A technology of hair follicle stem cells and construction methods, which is applied in the field of adult stem cell banks, can solve problems such as limitations in the application of embryonic stem cells, and achieve the effects of good application prospects, convenient cell acquisition, and abundant sources

Inactive Publication Date: 2015-07-22
上海坤爱生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Embryonic stem cells, namely totipotent stem cells, have the most research value in terms of differentiation ability, but they are derived from embryos, and there are bound to be ethical and safety issues (potential tumorigenicity), so the application of embryonic stem cells is limited

Method used

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  • Construction method for human hair follicle stem cell bank
  • Construction method for human hair follicle stem cell bank
  • Construction method for human hair follicle stem cell bank

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The operations in this example were all carried out in a strictly sterile environment.

[0035] The construction method of the human hair follicle stem cell bank provided in this embodiment comprises the following steps:

[0036] 1) Collection of donor hair: Before collecting donor hair, the donor must sign an informed consent before collection. The informed consent form includes information such as the donor's name, age, ID number, mailing address, and contact information. After signing the consent form, the donor is tested for ABO / Rh blood type, HLA typing and HIV, HBV, HCV, and then the donor's hair is collected under aseptic conditions; in other embodiments of the present invention, also In addition to the above-mentioned information collection and testing, it is possible to carry out the information that the collector thinks needs to be collected and other tests that are required;

[0037] 2) Acquisition and isolation of human hair follicle stem cells: under ster...

Embodiment 2

[0045] The construction method of the human hair follicle stem cell bank provided in this embodiment comprises the following steps:

[0046] 1) Collection of donor hair: Before collecting donor hair, the donor must sign an informed consent before collection. The informed consent form includes information such as the donor's name, age, ID number, mailing address, and contact information. After signing the consent form, the donor is tested for ABO / Rh blood type, HLA typing and HIV, HBV, HCV, and then the donor's hair is collected under aseptic conditions; in other embodiments of the present invention, also In addition to the above-mentioned information collection and testing, it is possible to carry out the information that the collector thinks needs to be collected and other tests that are required;

[0047] 2) Acquisition and isolation of human hair follicle stem cells: under sterile conditions, the hair with intact dermal papilla was picked, the root of the hair follicle wa...

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PUM

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Abstract

The invention discloses a construction method for a human hair follicle stem cell bank. The method comprises the steps of collecting the hair of a donor, acquiring and separating human hair follicle stem cells, cultivating the human hair follicle stem cells, detecting and cryopreserving the human hair follicle stem cells, constructing the bank and the like. According to the method, the human hair follicle stem cells can be effectively separated, purified and amplified, the current situation that such resources cannot be fully utilized is changed, and the stem cell bank for storing the human hair follicle stem cells is established to provide a large number of stem cell resources for clinical and scientific research application; the stem cell bank is constructed according to the acquired human hair follicle stem cells, and is used for storing hair follicle stem cells of an healthy adult and providing clinically applicable human hair follicle stem cells when the healthy adult requires stem cell treatment when being ill, and the stored human hair follicle stem cells can be used for producing a stem cell preparation with permission.

Description

technical field [0001] The invention relates to a human hair follicle-derived adult stem cell bank, that is, a method for constructing a human hair follicle stem cell bank. Background technique [0002] Stem cells (stem cells, SC) refer to primitive cells with self-renewal, high proliferation and multi-lineage differentiation potential, and can be divided into embryonic stem cells (ESCs) and adult stem cells (adult stem cells, ASCs) according to their differentiation ability. Embryonic stem cells, namely totipotent stem cells, have the most research value in terms of differentiation ability, but they are derived from embryos, and there are bound to be ethical and safety issues (potential tumorigenicity), so the application of embryonic stem cells is limited. Although adult stem cells are weaker than embryonic stem cells in differentiation ability, they avoid ethical and safety issues, and autologous adult stem cell transplantation can also solve the problem of immune rejecti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/074A01N1/02
Inventor 齐念民高剑
Owner 上海坤爱生物科技股份有限公司
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