Methods for restoring dryness or proliferative ability of hair follicle-derived cells

A technology of proliferative ability and dermal papilla cells, which is applied in the field of restoring hair follicle-derived cell stemness or proliferation ability, can solve the problems of human body side effects and insignificant effects, and achieve the goal of solving hair loss, restoring hair follicle-derived cell stemness, and broad application prospects Effect

Inactive Publication Date: 2020-04-07
浙江卫未生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, minoxidil and finasteride mainly have good curative effect on androgenetic alopecia, but they have certain limitations, and require long-term medication, which w...

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  • Methods for restoring dryness or proliferative ability of hair follicle-derived cells

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Embodiment 1

[0033] A method for restoring stemness or proliferative ability of hair follicle-derived cells, comprising the following steps:

[0034] S1. Cut up the umbilical cord from which the arteries and veins have been removed, and wash thoroughly;

[0035] S2. Add low-sugar DMEM medium containing bovine serum and green chain double antibody, place in CO 2 Cultivate in the incubator, change the medium every 3 days;

[0036] S3. After the fusion of the mesenchymal stem cells reaches 80%, digest with trypsin and carry out subculture;

[0037] S4. Passage the mesenchymal stem cells to the fifth generation, and when the cell fusion reaches 80%, replace the medium without exosome serum to continue culturing;

[0038] S5. Collect the cell supernatant, centrifuge to remove cells or cell debris, and extract exosomes from mesenchymal stem cells by ultracentrifugation;

[0039] S6. Using the improved two-step enzymatic method to separate and cultivate the dermal papilla cells, the specific s...

Embodiment 2

[0046] A method for restoring stemness or proliferative ability of hair follicle-derived cells, comprising the following steps:

[0047] S1. Cut up the umbilical cord from which the arteries and veins have been removed, and wash thoroughly;

[0048] S2. Add low-sugar DMEM medium containing bovine serum and green chain double antibody, place in CO 2 Cultivate in the incubator, change the medium every 3 days;

[0049] S3. After the fusion of the mesenchymal stem cells reaches 80%, digest with trypsin and carry out subculture;

[0050] S4. Passage the mesenchymal stem cells to the fifth generation, and when the cell fusion reaches 80%, replace the medium without exosome serum to continue culturing;

[0051] S5. Collect the cell supernatant, centrifuge to remove cells or cell debris, and extract exosomes from mesenchymal stem cells by ultracentrifugation;

[0052] S6. Using the improved two-step enzymatic method to separate and cultivate the dermal papilla cells, the specific s...

Embodiment 3

[0059] A method for restoring stemness or proliferative ability of hair follicle-derived cells, comprising the following steps:

[0060] S1. Cut up the umbilical cord from which the arteries and veins have been removed, and wash thoroughly;

[0061] S2. Add low-sugar DMEM medium containing bovine serum and green chain double antibody, place in CO 2 Cultivate in the incubator, change the medium every 3 days;

[0062] S3. After the fusion of the mesenchymal stem cells reaches 80%, digest with trypsin and carry out subculture;

[0063] S4. Passage the mesenchymal stem cells to the fifth generation, and when the cell fusion reaches 80%, replace the medium without exosome serum to continue culturing;

[0064] S5. Collect the cell supernatant, centrifuge to remove cells or cell debris, and extract exosomes from mesenchymal stem cells by ultracentrifugation;

[0065] S6. Using the improved two-step enzymatic method to separate and cultivate the dermal papilla cells, the specific s...

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Abstract

The invention discloses a method for restoring the dryness or proliferative ability of hair follicle-derived cells, comprising the following steps: cutting umbilical cord with removed arteries and veins, culturing by adding a low-sugar DMEM culture solution containing bovine serum and penicillin-streptomycin, and carrying out subculture by trypsinization when mesenchymal stem cell fusion reaches 70-80%; continuously culturing by replacing a medium without exosome serum when the cells are passaged to the 3rd to 5th generation and cell fusion reaches 60-80%; extracting by a ultracentrifugation method to obtain exosomes of mesenchymal stem cells; carrying out isolated culture on dermal papillae cells by an improved two-step enzymatic method; and culturing the obtained dermal papillae cells ina DEME basal medium, which contains PDGF, KGF, VEGF and exosomes of mesenchymal stem cells. The method of the invention can effectively restore the dryness of hair follicle-derived cells, and regulates the normal physiological metabolism of hair follicle cells by secreting various regulatory factors, thus solving the problem of alopecia from the perspective of hair follicle physiology.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for restoring the stemness or proliferation ability of hair follicle-derived cells. Background technique [0002] Hair loss has become a common "modern disease" today. The reasons and types of hair loss are different, whether it is because of stress or pathological reasons, whether it is seborrheic alopecia or postpartum alopecia, all of them bring great trouble and inconvenience to people's lives. Although hair loss is not a serious disease, it will have a greater impact on the patient's psychology. Although the current understanding and understanding of hair loss is increasing, and supporting or adjuvant treatment measures are emerging in an endless stream, it is still expected to find more effective and comprehensive treatment methods in clinical practice. [0003] At present, the medicines used to treat hair loss at home and abroad are mainly western medicines, such as...

Claims

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Application Information

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IPC IPC(8): C12N5/071
CPCC12N5/0627C12N2502/1352
Inventor 陈锦阳袁博吴小佳
Owner 浙江卫未生物医药科技有限公司
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