Kit for detecting glycocholic acid content in serum and preparation method of kit

Abstract

The invention discloses a kit for detecting the glycocholic acid content in serum. The kit comprises a resistant reagent A, a resistant reagent B, a magnetic particle reagent, a calibration product, a quality control product, luminous substrate liquid and concentrated washing liquid; the resistant reagent A is a glycocholic acid derivative resistant reagent A marked with alkaline phosphatase; the resistant reagent B is a glycocholic acid antibody marked with FITC; the magnetic particle reagent is carboxyl magnetic particles coated with FITC antibodies. The invention further discloses a preparation method of the kit. According to the kit, a reaction system close to a homogeneous phase is provided by combining a chemiluminiscence technology with the immune magnetic particles; in addition, a one-step reaction mode is adopted, so that the detection properties (such as the flexibility, the precision and the detection range) are greatly improved, the reaction time is greatly shortened, and the time from sample adding to detection result obtaining is shorter than 35 min and obviously shorter than that of a kit of the same kind; the coupling efficiency is high, bonding is firm, the process is stable, and the product cost is greatly reduced while the product performance is improved.

Description

technical field [0001] The invention relates to the technical field of biochemistry, in particular to a kit for measuring the content of glycocholic acid (CG) in a human body by using an immunocompetition method and a magnetic particle chemiluminescence method. Background technique [0002] Cholyglycine (CG), a conjugated cholic acid formed by the combination of cholic acid and glycine, is one of the main components of bile acids. Cholesterol is transformed into primary bile acids, including cholic acid (CA) and chenodeoxycholic acid (CDCA), through extremely complex enzymatic reactions in liver cells. When the liver cells are damaged and the liver is diseased, the metabolism and circulation of glycocholic acid will be disturbed, and the amount of hepatic bile acid in the serum will increase. ), glutamyl transpeptidase (GGT) and serum albumin (ALB) and other routine liver function indicators are more sensitive. The levels of glycocholic acid in patients with various liver ...

AI Technical Summary

Problems solved by technology

The steps of radioimmunoassay are cumbersome, the reagents are expensive, and supporting equipment is required and there is radioactive contamination

Enzyme-linked immunosorbent assay has long detection time, complicated operation, poor repeatability, and is not suitable for emergency and clinical patients in time for diagnosis.

Latex-enhanced immunoturbidimetric method is simple and fast to operate, but has low sensitivity and poor reproducibility at low values

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