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Kit for detecting glycocholic acid content in serum and preparation method of kit

A technology of glycocholic acid and kits, applied in the field of biochemistry, can solve the problems of uncomfortable emergency and timely diagnosis of clinical patients, long detection time, low value repeatability, etc., to achieve the guarantee of validity period and detection performance, and shorten the reaction time , Improve the effect of product performance

Inactive Publication Date: 2017-01-04
TAIZHOU ZECEN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The steps of radioimmunoassay are cumbersome, the reagents are expensive, and supporting equipment is required and there is radioactive contamination
Enzyme-linked immunosorbent assay has long detection time, complicated operation, poor repeatability, and is not suitable for emergency and clinical patients in time for diagnosis.
Latex-enhanced immunoturbidimetric method is simple and fast to operate, but has low sensitivity and poor reproducibility at low values

Method used

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  • Kit for detecting glycocholic acid content in serum and preparation method of kit
  • Kit for detecting glycocholic acid content in serum and preparation method of kit
  • Kit for detecting glycocholic acid content in serum and preparation method of kit

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Experimental program
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Effect test

Embodiment

[0047] 1. Sample collection

[0048] Use correct medical techniques to collect serum (severe hemolysis or lipemia samples cannot be used for determination), and the collected samples should not be kept at room temperature for more than 8 hours; if not tested within 8 hours, the samples should be placed in a refrigerator at 2-8°C ; If it needs to be stored or transported for more than 72 hours, it should be frozen at below -20°C and avoid repeated freezing and thawing. Return to room temperature before use, shake gently to mix.

[0049] 2. Preparation before experiment

[0050] ① Take a bottle of concentrated lotion and dilute it 15 times with distilled water;

[0051] ② Adjust the temperature of the incubator or water bath to 37°C, and use it after the temperature is stable;

[0052] ③ Fully mix the magnetic particle suspension until there is no visible precipitation.

[0053] 3 Experimental methods

[0054] ① Take out a certain amount of reaction containers (flat-bottome...

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Abstract

The invention discloses a kit for detecting the glycocholic acid content in serum. The kit comprises a resistant reagent A, a resistant reagent B, a magnetic particle reagent, a calibration product, a quality control product, luminous substrate liquid and concentrated washing liquid; the resistant reagent A is a glycocholic acid derivative resistant reagent A marked with alkaline phosphatase; the resistant reagent B is a glycocholic acid antibody marked with FITC; the magnetic particle reagent is carboxyl magnetic particles coated with FITC antibodies. The invention further discloses a preparation method of the kit. According to the kit, a reaction system close to a homogeneous phase is provided by combining a chemiluminiscence technology with the immune magnetic particles; in addition, a one-step reaction mode is adopted, so that the detection properties (such as the flexibility, the precision and the detection range) are greatly improved, the reaction time is greatly shortened, and the time from sample adding to detection result obtaining is shorter than 35 min and obviously shorter than that of a kit of the same kind; the coupling efficiency is high, bonding is firm, the process is stable, and the product cost is greatly reduced while the product performance is improved.

Description

technical field [0001] The invention relates to the technical field of biochemistry, in particular to a kit for measuring the content of glycocholic acid (CG) in a human body by using an immunocompetition method and a magnetic particle chemiluminescence method. Background technique [0002] Cholyglycine (CG), a conjugated cholic acid formed by the combination of cholic acid and glycine, is one of the main components of bile acids. Cholesterol is transformed into primary bile acids, including cholic acid (CA) and chenodeoxycholic acid (CDCA), through extremely complex enzymatic reactions in liver cells. When the liver cells are damaged and the liver is diseased, the metabolism and circulation of glycocholic acid will be disturbed, and the amount of hepatic bile acid in the serum will increase. ), glutamyl transpeptidase (GGT) and serum albumin (ALB) and other routine liver function indicators are more sensitive. The levels of glycocholic acid in patients with various liver ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533G01N33/535G01N33/551G01N33/574G01N33/576
CPCG01N33/533G01N33/535G01N33/551G01N33/57438G01N33/576G01N2800/085
Inventor 季连星刘振国王赟孙裔雷孔晓凯丁文健夏振伟
Owner TAIZHOU ZECEN BIOTECH CO LTD
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