Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Glycocholic acid detection kit

A technology for detecting kits and glycocholic acid, applied in measuring devices, color/spectral characteristic measurement, instruments, etc., can solve the problems of poor reagent stability, cumbersome operation, low sensitivity, etc., and achieve the effect of low cost and simple preparation steps

Pending Publication Date: 2019-03-08
ZYBIO INC
View PDF5 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Radioimmunoassay requires professional radioimmunotherapy facilities, which are difficult to carry out in ordinary laboratories, and the accuracy of radioimmunoassay is low, and radioactive rays will also cause great harm to the health of operators, so it is rarely used in the world
The chemiluminescence method has high sensitivity, but the stability of the reagent is poor, and it requires expensive special chemiluminescence detection equipment, which is not conducive to the development of routine laboratories, and has obvious limitations in clinical application.
Enzyme-linked immunoassay is generally used for semi-quantitative determination, and the operation is cumbersome, the detection time is long, the degree of automation is low, and the repeatability is poor, which is not conducive to being widely used in clinical testing and emergency needs. , fast, but low sensitivity, poor repeatability of low values, narrow linear range

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Glycocholic acid detection kit
  • Glycocholic acid detection kit
  • Glycocholic acid detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Screening experiment of specific component concentration and formula ratio in the present invention

[0084] 1. Concentration screening experiment of 8-aniline-1-naphthalenesulfonic acid

[0085] 1.1 Experimental method:

[0086] The following experiment was designed to investigate the effect of 8-aniline-1-naphthalenesulfonic acid in glycocholic acid (latex enhanced immune turbidimetric method) kit. Add 8-aniline-1-naphthalenesulfonic acid to the R1 solution, and finally prepare to contain 8-aniline-1-naphthalenesulfonic acid with final concentrations of 0mg / mL, 0.05mg / mL, 0.10mg / mL, 0.15mg / mL , 0.20mg / mL, 0.25mg / mL, 0.30mg / mL solutions. The above seven groups of solutions were used as the R1 reagent in the self-made CG (latex enhanced immunoturbidimetric method) reagents, matched with the self-made R2 reagents and calibration quality control products, using the two-point endpoint method as the analysis method, and setting the rising reaction as the reaction direction. SPL...

Embodiment 2

[0127] A glycocholic acid kit, including the following components:

[0128] Glycocholic acid R1 reagent:

[0129]

[0130] Glycocholic acid R2 reagent:

[0131]

[0132] The preparation method of glycocholic acid-protein conjugate in this kit includes the following steps:

[0133] 1) Weigh the pure glycocholic acid and dissolve it with DMF to prepare a 10 mg / mL glycocholic acid solution; use DMF to prepare 8 mg / mL NHS solution; use DMF to prepare 10 mg / mL EDC solution; use ultrapure water to prepare 10 mg / mL cattle Serum albumin solution;

[0134] 2) Take 45ul and 33ul of NHS solution and EDC solution respectively, add them to 70ul glycocholic acid solution and stir for 30min at room temperature;

[0135] 3) Add the mixed solution obtained in step 2) to 0.5ml bovine serum albumin solution and stir for 12h at room temperature;

[0136] 4) Place the mixture obtained in step 3) in 0.01 mol / L citrate buffer at pH 6.0, and dialyze for 12 hours to obtain glycocholic acid-protein conjugate.

[0...

Embodiment 3

[0143] Glycocholic acid R1 reagent:

[0144]

[0145]

[0146] Glycocholic acid R2 reagent:

[0147]

[0148] The preparation method of glycocholic acid-protein conjugate in this kit includes the following steps:

[0149] 1) Weigh pure glycocholic acid, dissolve it with DMF to prepare 30mg / mL glycocholic acid solution; prepare 15mg / mL NHS solution with DMF; prepare 40mg / mL EDC solution with DMF; prepare 40mg / mL cattle with ultrapure water Serum albumin solution;

[0150] 2) Take 40.8ul and 30ul of NHS solution and EDC solution respectively, add them to 63.6ul glycocholic acid solution, and stir for 30min at room temperature;

[0151] 3) Add the mixed solution obtained in step 2) to 0.9ml bovine serum albumin solution, and stir for 12h at room temperature;

[0152] 4) Place the mixture obtained in step 3) in 0.01 mol / L citrate buffer at pH 6.0, and dialyze for 12 hours to obtain glycocholic acid-protein conjugate.

[0153] The preparation method of anti-glycolic acid antibody coated latex...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a glycocholic acid detection kit and a preparation method of a glycocholic acid-protein conjugate and glycocholic acid antibody coated rubber latex particles in the glycocholicacid detection kit. According to the glycocholic acid detection kit, glycocholic acid is detected by a competition method, 8-aniline-1-naphthalene sulfonic acid is added into the kit, so that the analysis sensitivity and precision of the kit are obviously improved, the linear range of detection is widened, preparation steps of specific substances in the kit are simple, and the kit is low in cost and more applicable to industrial production and clinic application.

Description

Technical field [0001] The invention relates to the technical field of in vitro detection, in particular to a reagent for detecting glycocholic acid and a preparation method thereof. Background technique [0002] Serum Cholyglycine (CG) is one of the secondary bile acids that combine bile acid with glycine. It is synthesized by hepatocytes, and CG is synthesized by hepatocytes. It is discharged into the gallbladder through the capillary duct and bile duct, and enters the gallbladder along with the bile. Finger intestines, help food digestion. 95% of bile acid is reabsorbed in the terminal ileum, returns to the liver via the portal vein, and is taken up by hepatocytes for reuse. It mainly exists in the form of protein binding in serum. Under normal circumstances, the content of bile acid in the peripheral blood is very small, so the serum CG concentration of normal adults is stable at a low level regardless of fasting or after meals. When liver cells are damaged, the ability of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/531G01N33/543G01N21/31
CPCG01N21/31G01N33/531G01N33/54393G01N2800/085
Inventor 李元丽刘霖张艳军
Owner ZYBIO INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com