314 results about "Assay sensitivity" patented technology

The invention provides a full-scale CRP colloidal gold immunoturbidimetric assay kit. A quantitative assay purpose is reached through enhancing the turbidity by the colloidal gold. The method used by the kit provided by the invention solves a problem that the generation of a precipitate after a latex reinforced immunoturbidimetric reaction goes against biochemical instrument cleaning. The kit provided in the invention comprises a reagent R1 and a reagent R2, wherein the reagent R2 is a proper buffer solution; and the reagent R2 is a buffer solution of the colloidal gold combined with an antihuman CRP antibody. The kit has the characteristics of high sensitivity, strong specificity and good stability, can be used for assaying the content of the CRP in serum or blood plasma, and is suitable for clinical fully-automatic biochemical analyzers. The CRP assay sensitivity of the kit can reach 0.01mg / L, and the upper CRP assay limitation of the kit is 500mg / L.

The present invention relates to hydrophilic, high quantum yield acridinium compounds. It has been discovered that the placement of electron-donating groups in the acridinium ring system increases the amount of light that is emitted by the corresponding acridinium compound when its chemiluminescence is triggered by alkaline peroxide. More specifically, it has been found that the placement of one or two hydrophilic, alkoxy groups at the C-2 and / or C-7 position of the acridinium ring system of acridinium compounds increases their quantum yield and enhances the aqueous solubility of these compounds. The present hydrophilic, high quantum yield, acridinium compounds are useful chemiluminescent labels for improving the sensitivity of immunoassays.

The invention discloses an anti-mullerian hormone chemiluminescence immunoassay kit and a preparation method and application thereof. The anti-mullerian hormone chemiluminescence immunoassay kit comprises a solid-phase carrier enveloped by an anti-mullerian hormone monoclonal antibody, and an anti-mullerian hormone monoclonal antibody marked by a chemiluminescence marker. The anti-mullerian hormone chemiluminescence immunoassay kit can complete the assay of anti-mullerian hormone by taking a full-automatic chemiluminescence immunoassay analyzer as an assay tool. Through experiments, the assay sensitivity of the anti-mullerian hormone chemiluminescence immunoassay kit can reach 0.01ng / mL, and is improved by at last ten times compared with the traditional anti-mullerian hormone assay method, and the assay precision of the anti-mullerian hormone chemiluminescence immunoassay kit is higher.

The invention discloses a method for measuring glycerinum and 1,2-propylene glycol in cigarettes, electronic cigarettes and low-temperature cigarettes. The method comprises cigarette suction, smoke capturing, sample extraction and instrumental analysis and specifically comprises the steps: (1) sucking the cigarettes, the electronic cigarettes and the low-temperature cigarettes through a range hood; (2) capturing components to be measured in mainstream smoke through a Cambridge filtering disk or an absorption bottle; (3) adding a solvent into the Cambridge filtering disk with the components to be measured for extraction; (4) filtering an extracting solution or an absorption solution in the absorption bottle for analysis and determination through an instrument. The method is high in reproducibility and high in analysis sensitivity and can be used for accurately and quantitatively analyzing the glycerinum and the 1,2-propylene glycol in the cigarettes, the electronic cigarettes and the low-temperature cigarettes. The method is easy to operate, and a measurement result is accurate; therefore, the industrial standard for evaluation and monitoring of the quality of a cigarette product is further perfected.

Multiple development models of software applications utilizing different service environments may be annotated with additional information and transformed within a transformation chain into a resulting unified performance analysis model that may be used to evaluate the development models, for example, for simulations and / or analytical sensitivity analysis by utilizing different performance analysis environments. By relating elements of the development models through the transformation chain to elements of resulting unified models, the evaluation may be performed with respect to the resulting / transformed model, but provided to a user in terms of the original development models. In this way, a user of the development models may work with the more-familiar development models, utilizing multiple different performance analysis tools, without having to alter the development models directly in order to obtain the evaluation.

The invention discloses a quantitative creatine kinase-myoglobin (CK-MB) determination kit. The kit is characterized in that the kit contains CK-MB magnetic separation reagent, enzymatic reactant, reaction enhancer, diluent, CK-MB calibrator, CK-MB quality control material, cleaner concentrate and substrate solution. The invention also discloses a preparation method for the kit. The kit integrates the chemiluminescence technology with immunomagnetic beads to provide a homogeneous phase-approximating reaction system. Compared with the prior art, the kit has higher assay sensitivity and specificity, and achieves better performance parameters, and mover, the product cost is greatly reduced.

The present invention provides a plasma spectrochemical analysis method that can be carried out easily and achieves high analytical sensitivity, and includes: a step of concentrating an analyte in a sample in the vicinity of at least one of a pair of electrodes by applying a voltage to the pair of electrodes in the presence of the sample; and a step of generating plasma by applying a voltage to the pair of electrodes and detecting light emitted from the analyte excited by the plasma.

The invention discloses a common rapid detection method for various pesticide residues in soybeans. The common rapid detection method is characterized by particularly comprising the following steps: homogenizing and extracting a soybean sample by acetonitrile; carrying out salting-out and centrifuging to obtain a supernatant; purifying by using a solid phase extraction column to obtain a sampling solution; preparing a mixed standard solution and preparing a base material adding standard curve; simultaneously detecting 306 types of pesticide residues in the soybeans by a gas chromatography-tandem mass spectrometer; finally, calculating the concentration. The common rapid detection method has the advantages that the effect of the base material and background interferences are reduced and the anti-interference capability of the method is improved greatly; the analysis sensitivity is improved; the detection linear range is 0-0.2microgram / milliliter; the detection is limited to be 0.01mg / kg; the recycling rate is 60%-120%; the relative standard deviation RSD is less than or equal to 20%; the recycling rate is ideal and the precision degree is good; the common rapid detection method is simple and rapid, is low in background interferences, good in selectivity and high in sensitivity.

The invention discloses an electrochemical sensor used for instantly monitoring and detecting the water biotoxicity. The electrochemical sensor comprises a work electrode, a counter electrode, a reference electrode and a electrolytic cell; the work electrode is a polymer fixed microbial electrode, and the polymer fixed microbial electrode comprises an electrode and a polymer and bacteria mixed layer coated outside the electrode; and the material of the above polymer is gelatin or chitosan, the above bacteria comprise Escherichia coli and / or yeast, and the electrode is a glassy carbon electrode, a gold electrode, a platinum electrode or a boron-doped diamond film electrode. The invention also discloses an apparatus of the electrochemical sensor used for instantly monitoring and detecting the water biotoxicity. The electrochemical sensor can instantly monitor the water biotoxicity change and detect the water biotoxicity value, can realize instant, online and continuous detection, and has the characteristics of high analysis sensitivity, low cost, simple operation, portability and the like.

The invention belongs to the technical field of measurement of concentration, temperature, pressure or flow speed of gas and provides a method and a device for improving the detection precision of a tunable laser absorption spectrum. Under a condition that the expense of system hardware is not increased, all pieces of information of spectral lines can be completely acquired and recorded, so that the measurement precision of a TDLAS (tunable diode laser absorption spectroscopy) system is improved; furthermore, the method and the device are suitable for on-line (in-situ) or off-line detection or monitoring application in the concentration, the pressure, the temperature and the flow speed of the gas. According to the technical scheme disclosed by the invention, a method for improving the laser gas analysis sensitivity based on nonlinear tuning comprises the steps of detecting the absorption spectrum of the gas to laser, and measuring parameters including the concentration, the pressure, the temperature and the flow speed of the gas, wherein certain transformation is applied in a laser excitation stage, and corresponding inverse transformation is applied in the stage of detecting the absorption spectral lines of the gas to the laser. The method and the device are mainly applied to gas detection.

The invention relates to the field of insulating oil gas chromatography analysis equipment and in particular to a portable gas chromatograph analysis device comprising a casing; a sample pretreatmentmodule for separating a gas in an insulating oil sample container by an automatic headspace degassing device and quantitatively transferring the same to a chromatographic column box module through a sampling device and to a detector module; the chromatographic column box module including a chromatographic constant-temperature column box and a TCD detector, and used for subjecting the received gassample to chromatographic separation through an internal chromatographic column and transferring the same to the detector module; the detector module including an FID detector and a Ni catalytic converter which convert the component concentration after the chromatographic separation into an electrical signal and feed the electrical signal back to the controller in order that the controller amplifies the electrical signal, performs an analog-to-digital conversion process, and transmits a digital signal to a chromatographic workstation for data processing to obtain final chromatographic data. The portable gas chromatograph analysis device is high in sensitivity, small in volume, convenient to carry, efficient in time, and good in practicality and operability.

A mass spectrometer capable of realizing a high-sensitivity ion analysis and a high ion selectivity performance. The mass spectrometer includes the ion source where ions are produced, the ion trap where ions are accumulated, isolated, dissociated, and ejected, the detector to detect ions to be detected, and the controller to control operations of the ion trap. It has the features that the total ion accumulation in or just before each period is calculated based on the result obtained from the mass spectrometry in the preceding period, and that in at least one out of all periods, the condition of voltage applied to the ion trap is corrected depending on the total ion accumulation. Compared to the related art, the mass spectrometer of the present invention provides much improved performance in analysis sensitivity and ion selectivity.

The invention discloses a method for detecting alkaloids and nitrosamines in tobaccos simultaneously, and belongs to the technical field of tobacco chemical composition detection. The method comprises the working procedures of: sample extraction, purification, analysis and quantification. Firstly an internal standard solution and ultrapure water are added into tobacco leaf powder, extract liquor passes through a filter film after ultrasonic extraction, then methanol is added, protein is removed through centrifugation after shaking, and supernatant is subjected to liquid chromatography-mass spectrometry. After standard solutions of compounds to be detected with different concentration gradients are prepared by adopting a mixing preparation method, and the liquid chromatography-mass spectrometry is carried out, standard curves of instrument responses of the compounds to the actual concentrations of the solutions are drawn, curve equations are fitted, and the detection concentrations are calculated according to measured values of the instrument responses of the compounds to be detected and converted to the actual concentrations of the compounds to be detected in tobacco samples. The detection method provided by the invention can be used for carrying out the qualitative and quantitative analysis on alkaloids and nitrosamines in the tobacco samples simultaneously, and is easy and convenient to operate, good in reproducibility, high in analytic sensitivity and accurate in quantification.

The invention discloses a method and device for detecting an ultra trace sample based on surface-enhanced Raman spectroscopy (SERS). The device disclosed herein comprises an SERS optrode and a sample adaptive positioner having a groove structure with super-hyophobic characteristics. The method disclosed herein is characterized in that: a sample solution adheres to a super-hyophobic substrate without infiltration to form spherical liquid drops, but the sample solution adheres to the SERS optrode with infiltration; with the evaporation of the solvent, the sample liquid drops turns smaller and leave the super-hyophobic surface, solutes are concentrated and deposited at the head of the SERS optrode, thus the unit area concentration of the detected object is raised, and high-sensitivity analysis is realized. According to the invention, the amount (10-20 muL) of the required sample solution is few, the analysis sensitivity is high (e.g. the actual detection limit of pesticide Hostathion is only 20pg.), the method disclosed herein can be widely applied in the analysis and detection of trace materials in the fields of homeland security, environmental monitoring, food safety, medical health, etc.

Compositions, methods, devices, and systems are described for performing single-step, homogenous, localized surface plasmon resonance (LSPR)-based plasmonic assays having exceptional assay sensitivity and extremely low limits of detection (LODs). Ag / Au core / shell nanoparticles are described, which may be used with LSPR sensors to develop single-step, homogeneous, LSPR-based assays.

The invention discloses a double channel resonance enhancement laser induction penetrate spectrum trace element analyzer, which includes the laser, the monochromator, the photo detector, the oscilloscope and the computer, the states laser through the beam-splitting piece BS connects the sample channel and the photo source channel light signal; there is lens L1, the resonance photo source, coupling lens assembly L3 and L4 in turn installs on the photo source channel; there is reflector RM1, lens L2 in turn on the sample channel; the sample channel and photo source channel connect with the sample light signal, the sample through the light gather system connects the monochromator light signal, the monochromator connects the photo detector, the photo detector connects the oscillograph, the oscillograph connects the computer. The invention also discloses an analysis method of the double channel resonance enhancement laser induction penetrate spectrum trace element analyzer. The analyzer has simple structure and low cost, greatly enhanced the analysis sensitivity and reduced the examination limit.

The invention relates to a determination method for the content of carotenoid in wheat. The determination method comprises the following steps: (1) preparing a standard sample, namely mixing xanthophylls, zeaxanthin, alpha-carotene and beta-carotene standard substances to prepare a series of standard samples; (2) preparing a to-be-detected solution, namely, dissolving extracted carotenoid into a mixed solution of methyl alcohol and ethyl acetate with the ratio of 68 to 32 to obtain a mixed solution, centrifuging the mixed solution to obtain a centrifuging solution, and enabling the centrifuging solution to pass through an oily filter membrane so as to obtain the to-be-tested solution; (3) testing by adopting an ultra-high performance liquid chromatography, namely testing under a defined chromatographic condition; and (4) processing testing data, namely, drawing a standard curve, and substituting tested peak area data of the to-be-tested solution into the standard curve so as to obtain the content of each component of the carotenoid in the wheat. According to the determination method, the time spent on extracting the carotenoid in the wheat is short, and the efficiency is high; the adopted ultra-high performance liquid chromatography has the characteristics of rapidness, high efficiency, high analysis flexibility and the like, and the xanthophylls and the zeaxanthin can be separated completely; the determination method can be widely applied to the determination of the content of the carotenoid in the wheat.

The invention discloses a biological sample spectral analysis method based on a solute migration electrospray ionization technique. The biological sample spectral analysis method comprises the following steps: 1, under the conditions of normal temperature and normal pressure, firstly, injecting a solvent into a nanolitre spraying capillary tube; 2, loading a sample at the tail end of a solvent or in the middle of the solvent, applying direct-current voltage to the nanolitre spraying capillary tube so as to dissolve a target compound in the sample into the loaded solvent and migrate to the top end of the nanolitre spraying capillary tube, and furthermore performing electric spraying and detecting corresponding signals by using a mass spectrometry detector, thereby obtaining analysis result. The biological sample spectral analysis method has the characteristics that the operation is simple and convenient, no sample pretreatment is needed, the amount of the sample is small, the analysis sensitivity is high, real-time rapid analysis on the target compound is achieved, and the like.

The invention discloses a detection method and a device for volatile and semi-volatile components. The detection method for the volatile and semi-volatile components comprises the steps of collecting, preparing and analyzing samples and especially comprises the following steps of: performing catching by a filter disc, adding an organic solvent, and performing ultrasonic oscillation to obtain the sample; and performing liquid chromatographic separation on the sample, cutting flow-out components into a catching coil pipe, conveying the sample in the catching coil pipe into a gas chromatography (GC) device, adsorbing the sample by a solid phase extraction column, starting a gas chromatography-mass spectrometer (GC-MS), and analyzing components to be measured in a capillary separation column. The device is a liquid and gas phase bi-dimensional chromatography device, and solvent treatment between the liquid chromatography (LC) device and the gas chromatography device is solid phase extraction. According to the method and the device, the sample can enter a first-level LC with a large size, and fractions of the first-level LC can be cut into a second-level GC with a large size, so that as for the low-content samples, components separated by LC can be accumulated for many times, and then are transferred into the GC for analysis after being accumulated to an ideal analysis amount. Therefore, the samples can be efficiently purified, and the analysis sensitivity is greatly improved.

The present invention relates to novel methodologies for performing multiplexed assays with high precision and sensitivity. In particular, the present invention relates to improving assay sensitivity and precision by combining the normalization of multiplexed assay data using an internal standard with scattered application of samples and standards replicates throughout sample wells on a slide or set of slides as well as scattered replicates of arrayed probes in a single well. These compositions and methods can be used to perform multiplexed assays for analytes in patient and other test samples. In particular, these methods have applications for Quantitative Multi-analyte Immunoassays (QMI) to measure proteins in human serum and plasma.

Disclosed is a mass spectrometry apparatus and method capable of providing enhanced analysis sensitivity in a mass spectrometric analysis for a small amount of ions. A quadrupole rod-type ion guide is employed to temporarily accumulate ions to be introduced into an ion trap, and ions are introduced into the ion guide in an amount less than a saturated ion amount in the ion guide, and accumulated in an exit end of the ion guide. As compared with an octopole rod-type ion guide, the quadrupole rod-type ion guide has a higher ion-converging capability, and therefore can confine and hold a small amount of ions around an ion optical axis, although it is inferior in ion-accumulating capability. This makes it possible to efficiently introduce the ions into the ion trap through two openings of an electric field-correcting electrode and an entrance endcap electrode, so as to perform a high-sensitive analysis.

The invention provides an ELISA assay for the determination of serum mast cell β-tryptase levels using rabbit anti-tryptase as the capture antibody and alkaline phosphatase conjugated G3 as the detecting antibody. Luminescent substrate CPSD was used to enhance the assay sensitivity. Also provided are methods for aiding in the diagnosis of irritable bowel syndrome by detecting the serum level of β-tryptase, histamine and / or prostaglandin E2.

Belonging to the technical field of analytical chemistry, the invention discloses a method and device for detection of cigarette smoke benzo[alpha]pyrene. Including sample preparation and analysis, the detection method of benzo[alpha]pyrene specifically comprises: conducting trapping by a filter disc, adding an organic solvent to conduct ultrasonic shaking so as to obtain an analysis sample; separating the sample by a liquid chromatogram, cutting the needed outflow component into a gas chromatogram, and performing adsorption by a solid phase extraction column; and then starting GC-MS, and bringing the to-be-tested component into a capillary separation column to perform analysis. The device is a gas chromatograph-mass spectrometer with an on-line solid phase extraction purification function, wherein the solvent treatment between the liquid chromatogram and the gas chromatogram is solid phase extraction. With the device provided by the invention, efficient purification of the sample and larger volume sample injection of the gas chromatogram can be realized. For ultra low content samples, components subjected to liquid phase separation can be accumulated repeatedly to an ideal analysis amount and then transferred to the gas chromatogram for analysis. While achieving efficient purification of the sample, the analysis sensitivity is greatly improved.

Systems, methods, and devices related to detecting a presence of an analyte and / or determining a concentration of analytes are provided. An analyte may be provided on an LSPR-active surface. The LSPR-active surface may comprise sensitivity enhancing labels. The analyte may induce a local change near the LSPR-active surface. The LSPR-active surface may be imaged with an imaging device for images before, during, or after a reaction takes place. Local regions of interest within the images may be analyzed to detect the local changes.

The application provides a high-sensitivity magnetic particle chemiluminescence immunoassay kit for cardiac troponin I (hs-cTnI). The kit comprises a reagent R1, a reagent R2, a magnetic particle working solution and a cTnI antigen calibrator. The reagent R1 contains a biotin-labeled anti-cTnI polyclonal antibody; the reagent R2 contains two acridinium-ester-labeled anti-cTnI monoclonal antibodiesagainst different cTnI epitopes; the magnetic particle working solution includes streptavidin magnetic beads. In addition, the kit also can include a H2O2 solution and an alkaline solution. Besides,the application also provides a preparation method and purpose of the kit. The kit is capable of identifying multiple epitopes of the cTnI antigen, so that the analytical sensitivity and precision ofthe cardiac troponin I chemiluminescence immunoassay kit are improved.

The invention provides an ELISA assay for the determination of serum mast cell β-tryptase levels using rabbit anti-tryptase as the capture antibody and alkaline phosphatase conjugated G3 as the detecting antibody. Luminescent substrate CPSD was used to enhance the assay sensitivity. Also provided are methods for aiding in the diagnosis of irritable bowel syndrome by detecting the serum level of β-tryptase, histamine and / or prostaglandin E2.

The invention discloses a surface-enhanced Raman spectrum (SERS) substrate, and a preparation method thereof. The substrate comprises a flexible base layer and a noble metal layer. At least one face of the flexible base layer has a surface roughness like that of metallographic abrasive paper, and the noble metal layer is adhered to the flexible base layer, wherein the noble metal layer is formed by one or more materials selected from gold, solver, and copper. According to the invention, a noble metal surface with micrometer and nanometer double-roughness is directly formed on the base layer. The SERS substrate has ultra-high analytic sensitivity which is suitable for detections of ultra-trace samples, and has advantages of good reproducibility, simple production, short period, low cost, and suitability for batch productions. Therefore, the substrate and method provided by the invention can be widely applied in fields of homeland security, environmental monitoring, food safety, medical and health care, and the like.

The invention discloses a detection method and device for high-sensitivity and high-reproducibility surface enhanced Raman spectroscopy. The method that detection is performed repeatedly to obtain the average value is adopted, the problem of relatively high discreteness in the existing detection method is avoided, the method and the device have high reproducibility and ultrahigh analytical sensitivity, and are suitable for detection of ultratrace samples, detection results can be obtained accurately, the operation is simple, and the detection period is short. According to the method, a fine-tuning device is adopted, so that the movement of a substrate is achieved so as to perform multi-point detection, the device is simple in structure, lower in cost, and easy and convenient to operate; the method and the device provide conditions for further promoting the SERS technology to be widely used in the fields of homeland security, environmental monitoring, food safety, health care and the like.

The invention relates to a detection kit for ureaplasma urealyticum (UU) nucleic acid by utilizing RNA constant-temperature amplification. The detection kit comprises a urine sample preserving solution, a nucleic acid extraction solution, a UU reaction solution, a UU detection solution, an SAT (Spermidine / Spermine N1-Acetyltransferase) enzyme solution, a UU positive control and a UU negative control. The detection kit provided by the invention has the analytical sensitivity of 50 CFU / reaction for detecting UU, and has the characteristics of high specificity and high sensitivity; and an amplification product RNA is easy to degrade in a natural environment and causes low pollution.