311 results about "Assay sensitivity" patented technology

The invention provides a full-scale CRP colloidal gold immunoturbidimetric assay kit. A quantitative assay purpose is reached through enhancing the turbidity by the colloidal gold. The method used by the kit provided by the invention solves a problem that the generation of a precipitate after a latex reinforced immunoturbidimetric reaction goes against biochemical instrument cleaning. The kit provided in the invention comprises a reagent R1 and a reagent R2, wherein the reagent R2 is a proper buffer solution; and the reagent R2 is a buffer solution of the colloidal gold combined with an antihuman CRP antibody. The kit has the characteristics of high sensitivity, strong specificity and good stability, can be used for assaying the content of the CRP in serum or blood plasma, and is suitable for clinical fully-automatic biochemical analyzers. The CRP assay sensitivity of the kit can reach 0.01mg/L, and the upper CRP assay limitation of the kit is 500mg/L.

The invention discloses an anti-mullerian hormone chemiluminescence immunoassay kit and a preparation method and application thereof. The anti-mullerian hormone chemiluminescence immunoassay kit comprises a solid-phase carrier enveloped by an anti-mullerian hormone monoclonal antibody, and an anti-mullerian hormone monoclonal antibody marked by a chemiluminescence marker. The anti-mullerian hormone chemiluminescence immunoassay kit can complete the assay of anti-mullerian hormone by taking a full-automatic chemiluminescence immunoassay analyzer as an assay tool. Through experiments, the assay sensitivity of the anti-mullerian hormone chemiluminescence immunoassay kit can reach 0.01ng/mL, and is improved by at last ten times compared with the traditional anti-mullerian hormone assay method, and the assay precision of the anti-mullerian hormone chemiluminescence immunoassay kit is higher.

The invention discloses a method for measuring glycerinum and 1,2-propylene glycol in cigarettes, electronic cigarettes and low-temperature cigarettes. The method comprises cigarette suction, smoke capturing, sample extraction and instrumental analysis and specifically comprises the steps: (1) sucking the cigarettes, the electronic cigarettes and the low-temperature cigarettes through a range hood; (2) capturing components to be measured in mainstream smoke through a Cambridge filtering disk or an absorption bottle; (3) adding a solvent into the Cambridge filtering disk with the components to be measured for extraction; (4) filtering an extracting solution or an absorption solution in the absorption bottle for analysis and determination through an instrument. The method is high in reproducibility and high in analysis sensitivity and can be used for accurately and quantitatively analyzing the glycerinum and the 1,2-propylene glycol in the cigarettes, the electronic cigarettes and the low-temperature cigarettes. The method is easy to operate, and a measurement result is accurate; therefore, the industrial standard for evaluation and monitoring of the quality of a cigarette product is further perfected.

The invention discloses a common rapid detection method for various pesticide residues in soybeans. The common rapid detection method is characterized by particularly comprising the following steps: homogenizing and extracting a soybean sample by acetonitrile; carrying out salting-out and centrifuging to obtain a supernatant; purifying by using a solid phase extraction column to obtain a sampling solution; preparing a mixed standard solution and preparing a base material adding standard curve; simultaneously detecting 306 types of pesticide residues in the soybeans by a gas chromatography-tandem mass spectrometer; finally, calculating the concentration. The common rapid detection method has the advantages that the effect of the base material and background interferences are reduced and the anti-interference capability of the method is improved greatly; the analysis sensitivity is improved; the detection linear range is 0-0.2microgram/milliliter; the detection is limited to be 0.01mg/kg; the recycling rate is 60%-120%; the relative standard deviation RSD is less than or equal to 20%; the recycling rate is ideal and the precision degree is good; the common rapid detection method is simple and rapid, is low in background interferences, good in selectivity and high in sensitivity.

The invention belongs to the technical field of measurement of concentration, temperature, pressure or flow speed of gas and provides a method and a device for improving the detection precision of a tunable laser absorption spectrum. Under a condition that the expense of system hardware is not increased, all pieces of information of spectral lines can be completely acquired and recorded, so that the measurement precision of a TDLAS (tunable diode laser absorption spectroscopy) system is improved; furthermore, the method and the device are suitable for on-line (in-situ) or off-line detection or monitoring application in the concentration, the pressure, the temperature and the flow speed of the gas. According to the technical scheme disclosed by the invention, a method for improving the laser gas analysis sensitivity based on nonlinear tuning comprises the steps of detecting the absorption spectrum of the gas to laser, and measuring parameters including the concentration, the pressure, the temperature and the flow speed of the gas, wherein certain transformation is applied in a laser excitation stage, and corresponding inverse transformation is applied in the stage of detecting the absorption spectral lines of the gas to the laser. The method and the device are mainly applied to gas detection.

The invention discloses a method for detecting alkaloids and nitrosamines in tobaccos simultaneously, and belongs to the technical field of tobacco chemical composition detection. The method comprises the working procedures of: sample extraction, purification, analysis and quantification. Firstly an internal standard solution and ultrapure water are added into tobacco leaf powder, extract liquor passes through a filter film after ultrasonic extraction, then methanol is added, protein is removed through centrifugation after shaking, and supernatant is subjected to liquid chromatography-mass spectrometry. After standard solutions of compounds to be detected with different concentration gradients are prepared by adopting a mixing preparation method, and the liquid chromatography-mass spectrometry is carried out, standard curves of instrument responses of the compounds to the actual concentrations of the solutions are drawn, curve equations are fitted, and the detection concentrations are calculated according to measured values of the instrument responses of the compounds to be detected and converted to the actual concentrations of the compounds to be detected in tobacco samples. The detection method provided by the invention can be used for carrying out the qualitative and quantitative analysis on alkaloids and nitrosamines in the tobacco samples simultaneously, and is easy and convenient to operate, good in reproducibility, high in analytic sensitivity and accurate in quantification.

Compositions, methods, devices, and systems are described for performing single-step, homogenous, localized surface plasmon resonance (LSPR)-based plasmonic assays having exceptional assay sensitivity and extremely low limits of detection (LODs). Ag/Au core/shell nanoparticles are described, which may be used with LSPR sensors to develop single-step, homogeneous, LSPR-based assays.

The invention discloses a double channel resonance enhancement laser induction penetrate spectrum trace element analyzer, which includes the laser, the monochromator, the photo detector, the oscilloscope and the computer, the states laser through the beam-splitting piece BS connects the sample channel and the photo source channel light signal; there is lens L1, the resonance photo source, coupling lens assembly L3 and L4 in turn installs on the photo source channel; there is reflector RM1, lens L2 in turn on the sample channel; the sample channel and photo source channel connect with the sample light signal, the sample through the light gather system connects the monochromator light signal, the monochromator connects the photo detector, the photo detector connects the oscillograph, the oscillograph connects the computer. The invention also discloses an analysis method of the double channel resonance enhancement laser induction penetrate spectrum trace element analyzer. The analyzer has simple structure and low cost, greatly enhanced the analysis sensitivity and reduced the examination limit.

The invention discloses a biological sample spectral analysis method based on a solute migration electrospray ionization technique. The biological sample spectral analysis method comprises the following steps: 1, under the conditions of normal temperature and normal pressure, firstly, injecting a solvent into a nanolitre spraying capillary tube; 2, loading a sample at the tail end of a solvent or in the middle of the solvent, applying direct-current voltage to the nanolitre spraying capillary tube so as to dissolve a target compound in the sample into the loaded solvent and migrate to the top end of the nanolitre spraying capillary tube, and furthermore performing electric spraying and detecting corresponding signals by using a mass spectrometry detector, thereby obtaining analysis result. The biological sample spectral analysis method has the characteristics that the operation is simple and convenient, no sample pretreatment is needed, the amount of the sample is small, the analysis sensitivity is high, real-time rapid analysis on the target compound is achieved, and the like.

The invention discloses a detection method and a device for volatile and semi-volatile components. The detection method for the volatile and semi-volatile components comprises the steps of collecting, preparing and analyzing samples and especially comprises the following steps of: performing catching by a filter disc, adding an organic solvent, and performing ultrasonic oscillation to obtain the sample; and performing liquid chromatographic separation on the sample, cutting flow-out components into a catching coil pipe, conveying the sample in the catching coil pipe into a gas chromatography (GC) device, adsorbing the sample by a solid phase extraction column, starting a gas chromatography-mass spectrometer (GC-MS), and analyzing components to be measured in a capillary separation column. The device is a liquid and gas phase bi-dimensional chromatography device, and solvent treatment between the liquid chromatography (LC) device and the gas chromatography device is solid phase extraction. According to the method and the device, the sample can enter a first-level LC with a large size, and fractions of the first-level LC can be cut into a second-level GC with a large size, so that as for the low-content samples, components separated by LC can be accumulated for many times, and then are transferred into the GC for analysis after being accumulated to an ideal analysis amount. Therefore, the samples can be efficiently purified, and the analysis sensitivity is greatly improved.

The present invention relates to novel methodologies for performing multiplexed assays with high precision and sensitivity. In particular, the present invention relates to improving assay sensitivity and precision by combining the normalization of multiplexed assay data using an internal standard with scattered application of samples and standards replicates throughout sample wells on a slide or set of slides as well as scattered replicates of arrayed probes in a single well. These compositions and methods can be used to perform multiplexed assays for analytes in patient and other test samples. In particular, these methods have applications for Quantitative Multi-analyte Immunoassays (QMI) to measure proteins in human serum and plasma.

Disclosed is a mass spectrometry apparatus and method capable of providing enhanced analysis sensitivity in a mass spectrometric analysis for a small amount of ions. A quadrupole rod-type ion guide is employed to temporarily accumulate ions to be introduced into an ion trap, and ions are introduced into the ion guide in an amount less than a saturated ion amount in the ion guide, and accumulated in an exit end of the ion guide. As compared with an octopole rod-type ion guide, the quadrupole rod-type ion guide has a higher ion-converging capability, and therefore can confine and hold a small amount of ions around an ion optical axis, although it is inferior in ion-accumulating capability. This makes it possible to efficiently introduce the ions into the ion trap through two openings of an electric field-correcting electrode and an entrance endcap electrode, so as to perform a high-sensitive analysis.

Belonging to the technical field of analytical chemistry, the invention discloses a method and device for detection of cigarette smoke benzo[alpha]pyrene. Including sample preparation and analysis, the detection method of benzo[alpha]pyrene specifically comprises: conducting trapping by a filter disc, adding an organic solvent to conduct ultrasonic shaking so as to obtain an analysis sample; separating the sample by a liquid chromatogram, cutting the needed outflow component into a gas chromatogram, and performing adsorption by a solid phase extraction column; and then starting GC-MS, and bringing the to-be-tested component into a capillary separation column to perform analysis. The device is a gas chromatograph-mass spectrometer with an on-line solid phase extraction purification function, wherein the solvent treatment between the liquid chromatogram and the gas chromatogram is solid phase extraction. With the device provided by the invention, efficient purification of the sample and larger volume sample injection of the gas chromatogram can be realized. For ultra low content samples, components subjected to liquid phase separation can be accumulated repeatedly to an ideal analysis amount and then transferred to the gas chromatogram for analysis. While achieving efficient purification of the sample, the analysis sensitivity is greatly improved.

Systems, methods, and devices related to detecting a presence of an analyte and/or determining a concentration of analytes are provided. An analyte may be provided on an LSPR-active surface. The LSPR-active surface may comprise sensitivity enhancing labels. The analyte may induce a local change near the LSPR-active surface. The LSPR-active surface may be imaged with an imaging device for images before, during, or after a reaction takes place. Local regions of interest within the images may be analyzed to detect the local changes.

The application provides a high-sensitivity magnetic particle chemiluminescence immunoassay kit for cardiac troponin I (hs-cTnI). The kit comprises a reagent R1, a reagent R2, a magnetic particle working solution and a cTnI antigen calibrator. The reagent R1 contains a biotin-labeled anti-cTnI polyclonal antibody; the reagent R2 contains two acridinium-ester-labeled anti-cTnI monoclonal antibodiesagainst different cTnI epitopes; the magnetic particle working solution includes streptavidin magnetic beads. In addition, the kit also can include a H2O2 solution and an alkaline solution. Besides,the application also provides a preparation method and purpose of the kit. The kit is capable of identifying multiple epitopes of the cTnI antigen, so that the analytical sensitivity and precision ofthe cardiac troponin I chemiluminescence immunoassay kit are improved.

The invention provides an ELISA assay for the determination of serum mast cell β-tryptase levels using rabbit anti-tryptase as the capture antibody and alkaline phosphatase conjugated G3 as the detecting antibody. Luminescent substrate CPSD was used to enhance the assay sensitivity. Also provided are methods for aiding in the diagnosis of irritable bowel syndrome by detecting the serum level of β-tryptase, histamine and/or prostaglandin E₂.

The invention discloses a surface-enhanced Raman spectrum (SERS) substrate, and a preparation method thereof. The substrate comprises a flexible base layer and a noble metal layer. At least one face of the flexible base layer has a surface roughness like that of metallographic abrasive paper, and the noble metal layer is adhered to the flexible base layer, wherein the noble metal layer is formed by one or more materials selected from gold, solver, and copper. According to the invention, a noble metal surface with micrometer and nanometer double-roughness is directly formed on the base layer. The SERS substrate has ultra-high analytic sensitivity which is suitable for detections of ultra-trace samples, and has advantages of good reproducibility, simple production, short period, low cost, and suitability for batch productions. Therefore, the substrate and method provided by the invention can be widely applied in fields of homeland security, environmental monitoring, food safety, medical and health care, and the like.

The invention discloses a detection method and device for high-sensitivity and high-reproducibility surface enhanced Raman spectroscopy. The method that detection is performed repeatedly to obtain the average value is adopted, the problem of relatively high discreteness in the existing detection method is avoided, the method and the device have high reproducibility and ultrahigh analytical sensitivity, and are suitable for detection of ultratrace samples, detection results can be obtained accurately, the operation is simple, and the detection period is short. According to the method, a fine-tuning device is adopted, so that the movement of a substrate is achieved so as to perform multi-point detection, the device is simple in structure, lower in cost, and easy and convenient to operate; the method and the device provide conditions for further promoting the SERS technology to be widely used in the fields of homeland security, environmental monitoring, food safety, health care and the like.

The invention relates to a detection kit for ureaplasma urealyticum (UU) nucleic acid by utilizing RNA constant-temperature amplification. The detection kit comprises a urine sample preserving solution, a nucleic acid extraction solution, a UU reaction solution, a UU detection solution, an SAT (Spermidine/Spermine N1-Acetyltransferase) enzyme solution, a UU positive control and a UU negative control. The detection kit provided by the invention has the analytical sensitivity of 50 CFU/reaction for detecting UU, and has the characteristics of high specificity and high sensitivity; and an amplification product RNA is easy to degrade in a natural environment and causes low pollution.