37 results about "Assay sensitivity" patented technology

The invention belongs to the technical field of immunodiagnosis, and relates to a detection card for quantitatively detecting human total IgE antibodies, which comprises a card shell and a test strip, and the test strip is arranged in the card shell; the test strip comprises a sample pad, a combination pad, a reaction film and an absorption pad which are arranged on a bottom plate, wherein an animal anti-human IgE monoclonal antibody marked by lanthanide fluorescent microspheres is arranged on the combination pad; a detection line coated with an animal anti-human IgE polyclonal antibody and a quality control line coated with an animal IgG antibody are arranged on the reaction film. The invention further provides a preparation method, a kit and application of the detection card for quantitatively detecting the human total IgE antibody. The method can effectively eliminate the interference of non-specific fluorescence, greatly improves the analysis sensitivity, and is simple and convenient to operate and low in cost.

The invention relates to the technical field of analysis and detection, in particular to a sensibilization method for analyzing heavy mass number elements by X-ray fluorescence spectrometry, which comprises the following steps: placing a sample to be detected in a grinding tank, adding ultrapure water and a grinding medium, and crushing by a circulating ball milling process until a superfine powder sample in a target particle size range is obtained; a large amount of liquid in a sample is rapidly removed by adopting a suction filtration method, the sample is dried in a sterile dust-free drying oven, the dried sample is ground again, and the method is suitable for analysis and detection of Ga, Br, Bi, Pb, Th, U, Ca, Y, Zr and Mo elements. According to the invention, a small amount of sample is processed by using a rapid and simple ultrafine crushing technology, the sample is rapidly crushed to a target particle size range, the problem of low heavy mass number element analysis sensitivity under the small amount of sample is overcome, the signal detection intensity is improved, and the heavy mass number element analysis sensitivity of the X-ray fluorescence spectrometry is effectively improved.

Disclosed herein are methods and systems for detection of microorganisms in a sample and the utilization of such methods for selecting and monitoring therapies. The specificity of indicator bacteriophage, such as Staphylococcus-specific bacteriophage, allows detection of a specific microorganism, such as Staphylococcus and the indicator signal may be amplified to optimize assay sensitivity.

The invention relates to the technical field of clinical medical examination, in particular to an immunochromatography test strip for enhancing near-infrared two-region fluorescence through supramolecular host-guest interaction and a preparation method of the immunochromatography test strip. The invention provides a preparation method of an immunochromatographic test strip. The preparation method comprises the following steps: preparing a rare earth nanoparticle (RENPs) labeling solution; preparing a microsphere combination pad; preparing a sample pad; coating an antibody with a nitrocellulose membrane; and assembling and manufacturing the card. The aggregation phenomenon and partial fluorescence quenching effect caused in the surface modification process of the rare earth microspheres are reduced by utilizing an efficient surface modification strategy of a supramolecular host compound, the fluorescence luminescence property of the rare earth microspheres is effectively improved, and meanwhile, the antibody labeling efficiency of the rare earth microspheres is further improved by utilizing a supramolecular self-assembly strategy; the prepared test strip is obviously improved in analysis sensitivity, has the advantages of simplicity in operation, low cost and short detection time, and is suitable for rapid and sensitive detection of low-concentration markers in complex biological samples.

A partition plate is arranged in a bottle cavity of the mobile phase continuous liquid adding device and divides the bottle cavity into a main cavity and a liquid supplementing cavity, the partition plate downwards extends to the position about one centimeter away from the bottom of the bottle, and the main cavity and the liquid supplementing cavity are communicated at the bottom of the bottle. A liquid supplementing opening leading to the liquid supplementing cavity is formed in the bottle cap, and a liquid supplementing channel is arranged between the bottle cap and the liquid supplementing cavity. And the supplemented mobile phase flows through the liquid supplementing channel, enters the liquid supplementing cavity and is further supplemented into the main cavity. The supplemented mobile phase and the mobile phase in the liquid supplementing cavity are mixed to generate bubbles, and the bubbles only float upwards in the liquid supplementing cavity, are blocked by the partition plate and cannot flow into the main cavity, so that the mobile phase in the main cavity is not influenced by the bubbles, cannot bring noise to the liquid chromatograph and cannot cause sharp noise peaks of an obtained chromatogram, and the quality of the liquid chromatograph is improved. And the analysis sensitivity of the liquid chromatograph is not reduced. The floating plug is arranged in the liquid supplementing cavity, and the floating plug floats upwards to plug the liquid supplementing channel along with supplementing of the mobile phase, so that the mobile phase in the liquid supplementing cavity is prevented from flowing back and overflowing, and the risk of leakage is avoided.

Disclosed herein are methods and systems for rapid detection of microorganisms in a sample without enrichment culture of the microorganisms. Also disclosed is a modified bacteriophage comprising a non-natural indicator gene in the late gene region. The indicator product is not a fusion protein. The specificity of infectious agents allows targeting of specific microorganisms, and the indicator signal can be enlarged to optimize assay sensitivity.

To provide methods for producing mutant bacteriophages for detection of LISTERIA.SOLUTION: Methods for the production of mutant bacteriophages with altered host range are disclosed herein. Additionally, methods and systems for rapid detection of microorganisms, such as Listeria spp., in a sample are disclosed herein. A genetically modified bacteriophage comprising an indicator gene in the late gene region is also disclosed. The specificity of the bacteriophage, such as a Listeria-specific bacteriophage, allows detection of a specific microorganism, such as Listeria spp., and an indicator signal may be amplified to optimize assay sensitivity.SELECTED DRAWING: None

Disclosed herein are methods and systems for rapidly detecting microorganisms, such as bacteria, in a sample. Also disclosed are genetically modified bacteriophages that contain an indicator gene encoding a subunit of an indicator protein. The specificity of the bacteriophage allows for detection of specific target bacteria and amplifies the indicator signal to optimize assay sensitivity.

The present disclosure provides a method of detecting an anti-lysosomal enzyme antibody in a human subject (e.g., in the presence of a circulating lysosomal enzyme) and a method of increasing the efficacy of an anti-lysosomal enzyme antibody assay (e.g., increasing assay sensitivity and circulating lysosomal enzyme tolerance), comprising pre-treating the biological sample of the subject with a base having a pH of about 11 or greater, and measuring the presence of the anti-lysosomal enzyme antibody in the biological sample of the subject.

The invention relates to the technical field of biological detection, in particular to development of a DTT-mediated CRISPR / Cas12a system and HPV (human papillomavirus) detection application. The DTT-mediated CRISPR / Cas12a system is constructed, the PAM-sequence-free nucleic acid is targeted, the nucleic acid detection range of the CRISPR / Cas12a system is remarkably expanded, and meanwhile the analysis sensitivity and specificity of the CRISPR / Cas12a system are improved.

Disclosed herein are methods and systems for detecting microorganisms in a sample and the use of such methods for selecting and monitoring therapies. The specificity of an indicator bacteriophage, such as a Staphylococcus specific bacteriophage, allows the detection of a particular microorganism, such as Staphylococcus, and the indicator signal can be amplified to optimize assay sensitivity.

The present application relates to the technical field of medical detection, in particular to a D-dimer detection kit and a detection method thereof.The present application provides a D-dimer detection kit, which is composed of reagent R1 and reagent R2.The reagent R1 is mainly composed of a buffer, a preservative, an inorganic salt, a surfactant and a protein stabilizer;the reagent R2 is mainly composed of a buffer, a protein protective agent, sucrose and a preservative.The experiment shows that the kit is easy to operate and has a short analysis time, and the kit also has high analysis sensitivity, optimal precision, good test accuracy and a wide linear range, and can be combined with an automatic analyzer, which is conducive to its wide use in clinical practice.

A method for combining restrictive proteolysis and immunoassays to specifically detect and quantify amyloid-genic XFLC in a biological fluid. This method is accomplished by the use of mAbs that enable the detection and quantification of neoepitopes on dLCCD biomarkers produced after restricted proteolysis of ZFLC, which is very advantageous for kinetically unstable or amyloid-genic LFLC. The methods do not depend on FLC primary sequences in variable and constant domains, and can be used to detect and quantify up to 99% of human XFLC. The clinical utility of the assay includes early detection in individuals suspected of having plasma cell disorders (MGUS, SMM, MM, IgM-AL and AL), differentiation from other amyloidosis such as ATTR, use in companion diagnosis, proving of target conjugation, assessment of treatment response, detection of MRD, detection of recurrence. Assays, such as MSD, lateral flow, mass spectrometry, are also implemented that quantify the resulting dLCCD biomarkers and / or can further increase assay sensitivity.

The disclosure provides the methods of detecting an anti-lysosomal enzyme antibody in a human subject (e.g., in the presence of a circulating lysosomal enzyme) and the methods of improving an efficacy of an anti-lysosomal enzyme antibody assay (e.g., improving the assay sensitivity and the circulating lysosomal enzyme tolerance), comprising pre-treating the biological sample of the subject with a base at pH of about 11 or greater and measuring the presence of the anti-lysosomal enzyme antibody in the biological sample of the subject.

Disclosed herein are methods and systems for rapid detection of microorganisms, such as bacteria, in a sample. Also disclosed is a genetically modified bacteriophage comprising an indicator gene encoding one subunit of an indicator protein. The specificity of the bacteriophage allows detection of specific target bacteria, and the indicator signal can be amplified to optimize assay sensitivity.

Described herein are methods for the detection of molecular residual disease (MRD) and cancer by analysis of cell‑free nucleic acids. The invention provides a capture panel comprising target‑specific probes that are directed to one or more genomic regions encompassing a spectrum of variant types, namely structural variants (SV), large insertions / deletions, phased single‑nucleotide variants, small insertions / deletions, multi‑nucleotide variants and single‑nucleotide variants. By contacting a nucleic‑acid sample derived from a subject with the probe panel, the method enriches variant‑bearing molecules and subjects the captured set to sequencing. Disease presence or the likelihood thereof is determined on the basis of each target region, optionally integrating epigenetic information (e.g., differential methylation) obtained from a tumour biopsy. The probe design is adapted to technical noise, allocating higher probe concentrations to low‑noise, high‑complexity variants, thereby reducing reliance on molecular barcodes, lowering required read depth and improving analytical sensitivity for both fixed‑panel and patient‑specific MRD assays.

Disclosed herein are methods and systems for rapid detection of microorganisms such as bacteria in a sample. A genetically modified bacteriophage is also disclosed which comprises an indicator gene encoding one subunit of an indicator protein. The specificity of the bacteriophage allows detection of a particular bacteria of interest and an indicator signal may be amplified to optimize assay sensitivity.

The application belongs to the technical field of electrochemiluminescence immunosensor, discloses a kind of electrochemiluminescence immunosensor for detecting tumor marker and application thereof, and the preparation system of electrochemiluminescence immunosensor for detecting tumor marker includes: graphite carbon nitride preparation module, working electrode pretreatment module, central control module, antigen standard solution preparation module, modified electrode preparation module, immunosensor preparation module, tumor marker detection module, sensor detection curve drawing module, data storage module, update display module.The electrochemiluminescence immunosensor for detecting tumor marker provided by the application has high analysis sensitivity, strong specificity, is simple to use and low in cost.The application improves the sensitivity of electrochemiluminescence immunosensor by dropping coating bovine serum albumin solution and tumor marker antigen standard solution on the surface of graphite carbon nitride working electrode, and greatly simplifies the electrode preparation process without enzyme and label.

The DNA molecular machine makes a major breakthrough in the fields of biosensing, bioimaging, early cancer diagnosis and the like. However, the current DNA molecular machine strategy is continuously challenged by insufficient analysis sensitivity, and a precise signal amplification program is often essential. Single-particle inductively coupled plasma mass spectrometry (SP-ICPMS) has excellent sensitivity, and background noise and detection signals can be effectively distinguished. In the research, a novel method is provided, and a 3D DNA molecular machine is constructed by using single nanoparticle counting and bispecific nuclease (DSN) assisted signal amplification and is used for detecting the invasive prostate cancer (PCa) biomarker miRNA-200c. The strategy is improved in sensitivity, the limit of detection (LOD) is 0.93 pM (28 amol), and the strategy is successfully applied to human serum sample analysis.

The invention provides a detection method for ziprasidone synthesis intermediate 3-(1-piperazinyl)-1,2-benzisothiazole hydrochloride, belonging to the technical field of drug quality analysis and detection. The invention selects acetonitrile and triethylamine-containing aqueous solution in a certain proportion as mobile phases, performs programmed gradient elution in coordination, and improves separation degree and analytical sensitivity; a diode array detector is adopted, and the 2008-2022Shimadzu Corporation LabSolutions software / similar software carried by the diode array detector is used to confirm the relative quantification of main impurities in the main product 3-(1-piperazinyl)-1,2-benzisothiazole hydrochloride; and the method provided by the invention has short analysis time, simple analysis operation and good repeatability and adaptability.

The disclosure provides the methods of detecting an anti-lysosomal enzyme antibody in a human subject (e.g., in the presence of a circulating lysosomal enzyme) and the methods of improving an efficacy of an anti-lysosomal enzyme antibody assay (e.g., improving the assay sensitivity and the circulating lysosomal enzyme tolerance), comprising pre-treating the biological sample of the subject with a base at pH of about 11 or greater and measuring the presence of the anti-lysosomal enzyme antibody in the biological sample of the subject.

The invention relates to the technical field of equipment performance evaluation, in particular to a blood detection equipment performance evaluation method and system based on big data, and the method comprises the steps: collecting real sample detection data of detection equipment in a preset time period; determining an upper critical region and a lower critical region, and establishing a critical sample database; calculating a critical resolution index based on the critical sample database, wherein the critical resolution index is obtained through weighted calculation of a critical precision index, a boundary separation index and a distribution continuity index; grouping the critical sample database according to matrix types, calculating critical resolution indexes, and comparing, analyzing, identifying and analyzing sensitivity differences; setting a sliding time window, obtaining critical resolution indexes of different time periods, obtaining an analysis sensitivity time sequence, carrying out trend analysis, outputting a performance degradation curve, and triggering performance degradation early warning; and fusing the critical resolution index, analyzing the sensitivity difference and the performance degradation curve, and outputting an equipment performance evaluation report.

An assay for detecting target extracellular vesicles in a sample effectively utilizes a copper shell grown on a gold nanostructure labeling the target extracellular vesicles to enhance a scattered signal, thereby improving assay sensitivity.

The invention discloses a method for synchronously measuring multiple endocrine disruptors in solid waste. The method comprises the following steps: collecting a solid waste sample, and refrigerating and storing the solid waste sample by adopting a colored sealed container; performing first vortex treatment on the solid waste sample by adopting a vortex reagent to obtain a to-be-extracted sample; performing ultrasonic extraction treatment on the to-be-extracted sample according to a first extraction condition to obtain a primary extraction product; determining a final object to be detected according to the turbidity condition of the primarily extracted product; setting chromatographic conditions and mass spectrum conditions, and performing HPLC-MS / MS measurement on the final to-be-measured substance based on the chromatographic conditions and the mass spectrum conditions to obtain content data of the 16 ED Cs in the solid waste sample; according to the method, ultrasonic extraction is combined with a high performance liquid chromatography-tandem mass spectrometry technology, related conditions are optimized, simple, convenient and rapid pretreatment steps are realized, and the analysis sensitivity, the recovery rate and the precision are improved.

The application relates to an electrospray ion source device and a mass spectrometer, the electrospray ion source device comprising: a hollow spray needle assembly, an air amplification mechanism, a headspace mechanism, the headspace mechanism being in communication with the amplification cavity, and the headspace mechanism being used for introducing compressed gas into the amplification cavity to increase the gas flow rate in the amplification cavity. In the working process of the above-mentioned electrospray ion source device, the sample forms charged droplets under the action of the hollow spray needle assembly and is sprayed into the amplification cavity through the inlet; at the same time, the headspace mechanism introduces compressed gas into the amplification cavity to increase the gas flow rate in the amplification cavity, the fast-flowing gas contacts the charged droplets, the volatilization of the solvent in the charged droplets is accelerated, the reaction molecules contact and react with the charged droplets or the gaseous sample to generate ions, and the ions or the charged droplets are driven to move towards the mass spectrometer sampling port structure, thereby improving the droplet desolvation effect and the ionization efficiency of the ion source, so as to be beneficial to improving the analysis sensitivity of the mass spectrometer.

An assay for detection of a target extracellular vesicle in a sample advantageously utilizes copper shells grown on gold nanostructures labeling the target extracellular vesicle to enhance the scattering signal and thereby improve assay sensitivity.

Disclosed herein are methods for producing a mutant bacteriophage having an altered range of hosts. In addition, the present application discloses methods and systems for the rapid detection of microorganisms in a sample, such as a plurality of species of the genus Listeria. Also disclosed is a genetically modified bacteriophage comprising an indicator gene in an advanced gene region. The specificity of a bacteriophage, such as a Listeria specific bacteriophage, allows the detection of a particular microorganism, such as a variety of species of Listeria, and the indicator signal can be amplified to optimize assay sensitivity.

The utility model relates to a serum amyloid protein A determination kit. The kit comprises a shell and a test strip, the test strip comprises a support plate, a sample pad, a combination pad, a reaction film and absorbent paper, the shell comprises an upper cover and a lower cover, and the upper cover is provided with a sample adding hole and an observation window; colloidal carbon marked by a first serum amyloid protein A monoclonal antibody is sprayed on the combination pad; the reaction film is provided with a detection line and a quality control line, the detection line is sprayed with a second serum amyloid protein A monoclonal antibody, and the quality control line is sprayed with a goat-anti-mouse second antibody; the sample pad, the combination pad, the reaction film and the absorbent paper are fixed on the surface of the support plate; the conjugate pad is overlapped with one end, close to the detection line, of the reaction film, the sample pad is overlapped with the other end of the conjugate pad, the absorbent paper is overlapped with one end, close to the quality control line, of the reaction film, and all overlapping distances are within a preset distance range; the test strip is fixed in the shell; the sample adding hole is formed above the sample pad; the observation window is arranged above the reaction film and is large enough to expose the detection line and the quality control line on the reaction film, so that the measurement sensitivity is improved.

1. The name of this design product: Protective column (4.6# rotary type). 2. Purpose of this design product: to improve the sensitivity and separation of chromatographic analysis and reduce flow path diffusion. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the design points: Stereoscopic drawing 1.

Embodiments of the present disclosure include apparatus, systems and methods related to sample analysis. Particular embodiments include a microfluidic electrochemical immunosensor that employ a unique finger-actuated mixer for rapid, ultrasensitive measurements of protein biomarkers. This mixer generates swirling microflows in the liquid sample, which accelerates biomolecular transport, enhances antibody-antigen reactions and promotes immunocomplex formation. In specific embodiments, mixing can be implemented during the incubation steps, which accelerates biomolecular transport and promotes immunocomplex formation, leading to enhanced analytical sensitivity and a shortened detection time.