Micro-fluidic apparatus integrated with PDMS film, manufacturing method and application thereof

A microfluidic and microfluidic chip technology, which is applied to laboratory containers, chemical instruments and methods, and photoplate-making processes on patterned surfaces. It can solve the problems of complex preparation steps, high cost, and harsh experimental conditions. , to achieve the effect of simple production steps, good air permeability and low cost

Inactive Publication Date: 2009-09-16
NANJING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods require complex mechanical equipment such as spin coaters and solid templates, and the thickness of PDMS films produced by these methods is more than 50 microns. The disadvantages are high cost, complicated preparation steps, harsh experimental conditions, and the inability to prepare thinner films. This limits the wide applicability of these methods

Method used

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  • Micro-fluidic apparatus integrated with PDMS film, manufacturing method and application thereof
  • Micro-fluidic apparatus integrated with PDMS film, manufacturing method and application thereof
  • Micro-fluidic apparatus integrated with PDMS film, manufacturing method and application thereof

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Embodiment Construction

[0023] Such as figure 1 As shown in (a), cut a piece of 2.5 cm x 4 cm PET film (2: PET hydrophobic substrate), print a 1.5 cm long x 150 micron wide toner pipe as a male mold (1: print pipe male mold), and The surrounding area is piled up with PDMS strips less than 0.4 cm wide and 0.4 cm high to form a pool. (b) Pour the PDMS prepolymer prepared by the PDMS body (Dow Corning Corporation, Midland-Michigan, USA) and its cross-linking agent (Dow Corning Corporation, Midland-Michigan, USA) at a mass ratio of 10:1 into the pool , so that the PDMS liquid level is about 0.4 cm (3: PDMS prepolymer). (c) 20 microliters of water droplets (4: water droplets) were dropped into the PDMS prepolymer, and after the water droplets settled to the bottom, they were put into an oven and cured at 60 degrees Celsius for 30 minutes. (d) After the PDMS is completely solidified, take it out from the oven, remove the remaining droplets, uncover the PDMS cured layer, that is, separate from the PET sub...

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Abstract

The invention relates to a micro-fluidic apparatus integrated with a polydimethyl siloxane (PDMS) film, which is structurally characterized in that a flat glass sheet is pasted with a PDMS sheet, the pasting face of the PDMS sheet and the glass is provided with a groove to form a micro-fluidic pipeline, the surface of the PDMS sheet on the upper part of the central position of the micro-fluidic pipeline is a concave spherical surface, the thickness of the PDMS sheet between the lowest point of the concave spherical surface and the micro-fluidic pipeline is less than or equal to 10 microns, the PDMS sheet at two ends of the micro-fluidic pipeline is respectively provided with pores, and the pores are communicated with the micro-fluidic pipeline so as to form a fluid storage tank. The micro-fluidic apparatus can be applied to experiments such as film evaporation and crystallization or biological molecule concentration and the like in a micro-fluidic system. Special equipment is not needed to manufacture the micro-fluidic apparatus, the manufacturing step is simple, the cost is low, and the micro-fluidic apparatus is easily popularized and used in common laboratories. The invention also discloses a method for manufacturing the micro-fluidic apparatus.

Description

technical field [0001] The invention relates to a microfluidic device, in particular to a method for making an integrated PDMS thin film microfluidic device and using it for crystallization in a microfluidic system. Background technique [0002] Microfluidic devices act as functional carriers in the microfluidic system, and the enrichment, separation or other functions in the microfluidic system are all completed by the microfluidic devices alone or in cooperation with each other. [0003] Solution crystallization in a microfluidic system, especially the crystallization of biomolecules such as protein solutions, can avoid the influence of the convective effect of crystallization in traditional macroscopic systems, and the tiny crystals grown in a microfluidic system can be analyzed online, avoiding the need to separate crystals. The impact and pollution on the crystal, and can save the amount of some expensive medicines. Therefore, it is of great significance to carry out t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J19/00B01D9/00B01L3/00B81B1/00B81C3/00
Inventor 夏兴华刘德晔余晓冬
Owner NANJING UNIV
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