The invention belongs to the technical field of micro
fluid control, particularly disclosing a micro
fluid control chip and a preparation method and application thereof. One side of the
chip is provided with a plurality of sample introduction holes, each sample introduction hole is communicated with a sample introduction runner; after meeting, each sample introduction runner is communicated with a main runner; the main runner is provided with a narrow runner; a micro-sphere of which the size is 0.5-1.5 mm is filled in front of the inlet of the narrow runner; the outlet of the main runner is communicated to a waste
liquid tank arranged at the other side of the
chip. The preparation method mainly comprises: firstly preparing a PDMS substrate; then, preparing a PDMS cover plate with an upper groove; sticking the substrate onto the cover plate to obtain a semi-finished product of the chip; carrying out modified treatment on each runner in the semi-finished product of the chip; finally, putting the micro-sphere in, controlling the charge quantity, and finishing production. The micro
fluid control chip of the invention has high solution mixing efficiency, stable
solution flow rate, small volume, convenient carrying and relatively simple manufacturing; when being used for detecting the density of triphosadenine, the micro fluid control chip has the advantages of high sensitivity, favourable detection effect and the like and can not be affected by environment
humidity.