146 results about "Normal laboratory" patented technology

The invention discloses directional knockout on sheep MSTN gene by applying the CRISPR/Cas9 technology, and a method for verifying the impact effect of directional knockout on skeletal muscle satellite cell differentiation. The concrete contents are as follows: firstly, target gene cloning; secondly, gRBA design and synthesis; thirdly, CRISPR/Cas9 gene knockout vector construction; fourthly, CRISPR/Cas9 gene knockout vector exogenous activity detection; fifthly, CRISPR/Cas9 gene knockout vector endogenous activity detection; sixthly, CRISPR/Cas9 gene knockout vector knockout effect detection. The method has the following advantages: firstly, the experimental period is shorter; secondly, the method is simple and practicable, has high repeatability, and can be performed in common laboratories; thirdly, active vector constructed through the method is small in toxic and side effects, and can be applied to the preparation and production of transgenic animals; fourthly, the method is small in non-specific shear, and obviously improves the gene targeting knockout efficiency; fifthly, the method is high in applicability.

The invention relates to an antibody chip kit for diagnosis of various tumors. The kit includes an antibody chip, a tumor marker standard substance mixture, a biotin-labeled tumor marker detection antibody mixture, and a fluorescein Cy3-labeled streptavidin, wherein the antibody chip includes a substrate, 16 kinds of tumor marker specific antibodies fixed on the surface of the substrate, and two kinds of positive controls, and the tumor marker standard substance mixture is a freeze-dried mixture obtained by mixing 16 kinds of standard tumor marker standard substances together according to a certain amount. The kit can detect 16 clinical commonly used tumor markers, overcomes the defects of complicated operation, single detection index, low sensitivity and the like in the prior art, has the advantages of being cheap, convenient, sensitive, accurate, high in throughput, and less in amount of samples, and can be popularized and large-scaled in common laboratories.

The invention discloses a protein chip kit for detecting inflammatory factors, which comprises a basement membrane (1) and a reactant and a detection agent (2). A plurality of kinds of specific antibodies are fixed on the basement membrane; the specific antibodies and the inflammatory factors can undergo an antigen-antibody reaction and each specific antibody is respectively fixed on the basementmembrane for forming a plurality of independent recognition loci; and the reactant and the detection agent are used for detecting whether substances capable of undergoing the antigen-antibody reaction with the specific antibodies exist in a sample to be detected or not by an array-ELISA method. The invention also discloses a method for preparing the protein chip kit for detecting the inflammatoryfactors, which comprises a step of fixing the specific antibodies on the basement membrane. The kit of the invention adopts protein chip technology, can detect 40 inflammatory factors, overcomes a plurality of shortcomings and has the advantages of low cost, convenient use, high sensitivity and accuracy, high flux, small using amount of samples, capability of being popularized in an ordinary laboratory, massive production and the like.

The invention relates to a micro-fluidic apparatus integrated with a polydimethyl siloxane (PDMS) film, which is structurally characterized in that a flat glass sheet is pasted with a PDMS sheet, the pasting face of the PDMS sheet and the glass is provided with a groove to form a micro-fluidic pipeline, the surface of the PDMS sheet on the upper part of the central position of the micro-fluidic pipeline is a concave spherical surface, the thickness of the PDMS sheet between the lowest point of the concave spherical surface and the micro-fluidic pipeline is less than or equal to 10 microns, the PDMS sheet at two ends of the micro-fluidic pipeline is respectively provided with pores, and the pores are communicated with the micro-fluidic pipeline so as to form a fluid storage tank. The micro-fluidic apparatus can be applied to experiments such as film evaporation and crystallization or biological molecule concentration and the like in a micro-fluidic system. Special equipment is not needed to manufacture the micro-fluidic apparatus, the manufacturing step is simple, the cost is low, and the micro-fluidic apparatus is easily popularized and used in common laboratories. The invention also discloses a method for manufacturing the micro-fluidic apparatus.

The invention provides a flexible ultraviolet light imprinting composite template and a preparation method thereof. The composite template adopts a three-layer structure and comprises a flexible supporting layer, a middle layer and a rigid structure layer, wherein the flexible supporting later is a low-elasticity-modulus polymer substrate; the middle layer is an ultraviolet light curing cross-linking agent of a mixture of a polyfunctionality organic sulfydryl monomer and a polyfunctionality organic olefin monomer; the rigid structure layer is an ultraviolet light curing cross-linking agent of a mixture of a polyfunctionality sulfydryl monomer containing silicon and a polyfunctionality olefin monomer containing silicon; and the surface of the rigid structure layer adopts a micro-nano imprinting structure. The lexible ultraviolet light imprinting composite template is cured and prepared in an ordinary laboratory environment, and vacuum or nitrogen protection is not required; when the ultraviolet light curing imprinting is performed, mechanical assistance is not required; and the composite template has the advantages as follows: the flexible template imprinting is self-adapted to the shape of the substrate, and the rigid template can duplicate a high-resolution nano-structure.

The invention relates to a method for preparing half-fin anchovy antibacterial peptides, which comprises the following steps of: homogenating fish into fish paste, adding pepsin into the fish paste according to a ratio of 1,000-1,300U/g under the condition that the pH value is 1.5-2.5, raising the temperature to 35-45DEG C, performing enzymolysis for 2 to 3 hours, heating enzymolysis liquid at the temperature of between 95 and 100DEG C for 5 to 15 minutes for killing enzyme, cooling to the temperature of between 3 and 5DEG C, centrifuging at a rate of 6,000 to 8,000r/min for 5 to 15 minutes, filtering, taking intermediate filtrate, and performing frozen storage or freeze-dried storage at -20DEG C at least. The antibacterial peptides is prepared from a pure natural low-value marine product, namely half-fin anchovy; the half-fin anchovy antibacterial peptides are rich in amino acid necessary for human bodies, and have stable physical and chemical properties; the antibacterial peptides prepared from food-derived protein through enzymolysis have high safety, good absorptivity and good antibacterial effect, and can serve as a nutritional and functional additive in foods or is developed into drug resistant bacteria antibiotics. The preparation method is simple, economic and easy to operate, can be carried out in a common laboratory, and is suitable for industrial expanded production.

The invention discloses a visual minitype fluid cavitation testing device. The visual minitype fluid cavitation testing device comprises a visual testing unit and a feed liquid unit for providing a test medium for the visual testing unit, wherein the visual testing unit comprises a vacuum chamber, a cavitation part, a camera and a flowmeter, wherein the cavitation part is positioned in the vacuum chamber, and the camera is positioned in the vacuum chamber and is used for collecting a real-time image in the cavitation part; the flowmeter is positioned in the vacuum chamber and is used for collecting the flow of a test medium; the feed liquid unit comprises a test medium storage tank, a pressure source, an air guiding piece communicated between the test medium storage tank and the pressure source as well as an infusion pipe communicated between the test medium storage tank and the cavitation part. The visual minitype fluid cavitation testing device provided by the invention has the advantages that the structure is simple, a heat insulation effect is good, the property is stable, the test data are exact, the cost is low, and the visual minitype fluid cavitation testing device is used for an experimenter in a common laboratory.

The invention relates to a lithium ion gel electrolyte based on a polymethyl methacrylate-polystyrene diblock copolymer, and a preparation method thereof. The technical scheme is characterized in that: the diblock copolymer comprises: 0.35-0.45 g of a polymer matrix, 0.55-0.65 g of a mixture, wherein the mixture comprises a plasticizer and a lithium salt, the concentration of the lithium salt in the plasticizer is 1 mol/L. The polymer matrix is the polymethyl methacrylate-polystyrene diblock copolymer synthesized by an atom transfer radical polymerization method. According to the present invention, the electrolyte system has good ionic conductivity; the required conditions by the preparation process are simple, and can be achieved by the ordinary laboratory, such that the method of the present invention is the method for preparing the gel electrolyte, wherein the method has characteristics of directness, simpleness, broad-applicability, and simple operation process.

The invention relates to a method for measuring the normal temperature resistivity and the resistance temperature coefficient of a conductor at variable temperature. The method is characterized by installing a two-point electronic temperature measurement probe on a standard bridge test stand, introducing a microhmmeter measuring signal and an electronic temperature measurement probe signal into a computer acquisition card, simultaneously and continuously recording resistance values and temperature changes by using a computer, changing the room temperature by using an indoor air-conditioning device, computing the resistivity by using the recorded resistance values at different temperature and the cross section area measured by a density method, obtaining the variable linear slope K of a resistor R relative to temperature T according to a fitting curve, and computing the slope K by using a resistivity temperature variation coefficient [alpha]. The method uses a universal measuring bridge with the electronic temperature probe and the air conditioner temperature change so as to solve a puzzle in common laboratory conductor resistivity and temperature coefficient measurement, and is simple in measurement procedures and high in efficiency compared with a standard measuring method.

The invention discloses a heparin affinity column as well as the preparation method and the application thereof. The heparin affinity column is prepared through the following method: agarose gel 6FF is taken as a solid phase carrier which is firstly activated and then is aminated and coupled with amidocyanogen after an epoxy group is coupled, the aminated agarose gel 6FF and the heparin form intermediate aldimine in methanol, and the intermediate product of aldimine is further subjected to reductive amination for forming stable chemical bonds, thereby acquiring the heparin-agarose gel 6FF. The affinity column preparing preparation with high efficiency is simple and short. Through adopting the combination of the rear aldehyde of the heparin and the amidocyanogen of aminated sepharose, the activity of the heparin is not influenced, thereby ensuring the affinity column to have higher affinity; the heparin coupling is stable, and the heparin can be repeatedly used; the reagent adopted by the method has low cost and no environmental pollution, thereby laying a foundation for sweepingly isolating and purifying materials with specific binding capacity to the heparin in common laboratories, and promoting the research on domestic relative fields.

The invention relates to the field of making biochips, in particular to a preparation method of an agarose gel microfluidic device. Particularly, the agarose gel microfluidic device is prepared by three steps of assigning microfilaments, pouring and solidifying, and wiredrawing. The agarose gel microfluidic device manufactured by the method has good sealing property, and the recovery rate of liquid is up to 95%. The agarose gel microfluidic device manufactured by the method can generate stable concentration gradients, and is used for observing interaction between cells and biochemical micro-environments, especially the interaction under a flow condition. The method has simple operation, and the microfluidic device can be manufactured in an ordinary lab without expensive precision instruments.

The invention discloses a kit for detecting phosphorylated epidermal growth factor receptor and the kit adopts the protein chip technology. The kit comprises a base film (1) fixed with several kinds of specific antibodies and reactant and detection agent (2), wherein specific antibodies and phosphorylated epidermal growth factor receptor can perform antibody-antigen reaction, each kind of specific antibodies are fixed on a plurality of independent recognition sites which are fixed on the base film; and the reactant and detection agent (2) are used to detect whether the substance which can perform antibody-antigen reaction with specific antibodies exists in a sample to be detected through the array-ELISA method. The invention also discloses a preparation method of the kit. By adopting the kit of the invention, 17 phosphorylated epidermal growth factor receptors in a plurality of samples can be detected, lots of defects of the prior art can be overcome and the kit has the advantages that the kit is cheap, convenient, sensitive and accurate, has high flux and less amount of sample and can be popularized in the ordinary laboratory and scaled, etc.

The invention discloses carbon nanodots with optical excitation active oxygen generating performance and a preparation method thereof. The preparation method of carbon nanodots with optical excitationactive oxygen generating performance belongs to the technical field of preparation of carbon nano materaisl (carbon dots). The preparation method comprises the following steps: preparing a carbondotaqueous solution at 60 DEG C by means of a mixed solution of dopamine and 3-aminopropyl triethoxy silane; and carrying out dialysis and purification to obtain silicon-doped carbon nanodots. The carbonnanodots with optical excitation active oxygen generating performance uniform and high in fluorescence intensity can be synthesized in a mesotherm condition, and have an important application prospect in bio-imaging and sensing. The synthetic method is simple and low in cost and can be finished in a common lab.

The invention discloses a preparation method of a standard sample of a high molecular material containing harmful substances, comprising the following steps of: firstly weighing a matrix high molecular material sample, adding an organic solvent, and completely dissolving the matrix high molecular material sample by using ultrasound; then adding a standard solution of the harmful substances to a high molecular material solution, stirring or using the ultrasound or whirling so that the solutions are uniformly mixed; pouring the solution to a polytetrafluoroethylene disk, taking a prepared sample out till the organic solvent is totally volatilized, shearing into pieces by using a stainless steel shear, and smashing by using a freezing grinder to obtain the standard sample of the high molecular material containing the harmful substances. The invention adopts a liquid phase method for preparation; the obtained standard sample has good uniformity; in addition, the operation process is simple and fast without additional equipment and can be carried out in a common laboratory.

The fibrinogen plate method for detecting thrombase, batroxobin and thrombase inhibitor includes preparing fibrinogen plate with agarose and fibrinogen and quantitative detection via the diffusion reaction of standard sample and detected sample on the plate. The detection method of the present invention has simple operation, high sensitivity, being intuitive and stable, no interference of the turbidity and color of the detected sample and low cost. The detection method may be realized simply in lab with thermotank.

The invention discloses an aluminium alloy section and a manufacture method thereof. The aluminium alloy section comprises the following elementary components of 87.5 percent of Al, 0.4 percent of Si, 1.1 percent of Fe, 0.75 percent of Mn, 0.8 percent of Mg, 2.1 percent of Zn, 4.4 percent of Cu, 0.1 percent of Ni and 0.1 percent of Ti. The manufacture method of the aluminium alloy section comprises the following process steps of: (1) melting an aluminium material, and adding metal components including the Si, the Fe, the Cu, the Mn, the Mg, the Zn, the Ni and the Ti; (2) controlling high temperature for casting to form an aluminium-based solid solution; (3) carrying out purification treatment and modification to enable grains to be refined and reduced to 1/20 before treatment and enable the total content to be reduced by 30 percent to obtain antirust hard aluminium alloy; and (4) splicing and molding by adopting a mould to obtain the aluminium alloy section. The aluminium alloy section provided by the invention has favorable corrosion resistance and excellent mechanical strength and is superior to a general section for a common laboratory.

The invention relates to a method for corn straw modification, in particular to a method for chemical modification to corn straws by using a chemical preparation. The method comprises the following steps: dry corn straws are washed by deionized water, cut into broken pieces and dried and crushed, the crushed corn straws are stirred with 800-1000 ml of sodium hydroxide solution for 4-6 hours at the temperature of 70-90 DEG C and then are filtered and washed, the filter residue is added with 50-100 ml of sodium hydroxide solution and 20-50 ml of ethanol solution after filtration and then reacts for 30-60 minutes at the temperature of 20-40 DEG C, the mixed liquor after reaction is added with 50-100 ml of ethanol solution and 30-50 ml of chloroacetic acid solution and reacts for 1-2 hours at the temperature of 70-90 DEG C and then is added with 20-50 ml of sodium hydroxide solution and 30-50 ml of ethanol solution, and the mixed liquor is cooled after being maintained for 60-100 minutes and then is titrated to be neutral with hydrochloric acid, filtered and washed with ethanol. The preparation method can be realized in the ordinary laboratory, a large-scale instrument is not required, the reaction conditions are accessible, the raw materials have low price and are easily obtained and the experimental wastewater can be reused.

The present invention relates to the low temperature packing method of glass substrate chip, and features that in the condition of existing hydrated layer in the packed surface of the micro analysis chip with glass substrate and in the ambiance of low temperature (40-200 deg.c) or room temperature (0-40 deg.c), the micro analysis chip with glass substrate is packed through low temperature heating or simple setting. The present invention has the advantages of simple and feasible technological process, low temperature or room temperature packing, and packing performance reaching the requirement of conventional electrophoretic separation experiment.

The invention discloses a preparation method of a micro-fluidic chip template. The preparation method includes the steps: first, preparing a micro-fluidic chip; second, taking the micro-fluidic chip prepared in the first step as a model, and casting with AB polyester resin to prepare the micro-fluidic chip template. The invention further discloses the micro-fluidic chip template and an applicationthereof. The application includes the steps: attaching the micro-fluidic chip template onto an organic glass plate to form an inner cavity; fixing the micro-fluidic chip template and the organic glass plate, pouring PDMS (polydimethylsiloxane) prepolymer into the inner cavity; solidifying the PDMS prepolymer; performing stripping to obtain a micro-fluidic chip completely consistent with an initially prepared micro-fluidic chip. The preparation method is simple and low in cost and can be finished in a common laboratory, materials are easily obtained, the punch position and the pipeline lengthof the original micro-fluidic chip are completely copied, and the micro-fluidic chips are integrally formed and good in repeatability.

The invention discloses a method for analyzing and determining an arsenic form in a liquid food seasoning, and is used for solving the problem that no sensitive, quick, accurate and low-cost method for analyzing and determining the arsenic form in the liquid food seasoning exists at present. The method takes a reactor, a cold trap, a thermostatic container, a hydrogen generator, an atomic fluorescence spectrometer and a chromatographic signal processor as measuring instruments, and comprises the following steps of a, generation of a gaseous arsenic compound; b, on-line cold trapping and separation of the gaseous arsenic compound; and c, detection of the gaseous arsenic compound. According to the method, a complicated pretreatment process of a sample is cancelled, so that the pretreatment cost is saved; the sample is added directly, and reacts immediately, and an overall determination process does not exceed 8 minutes, so that the determination period is shortened greatly; the accuracy and the sensitivity of analysis and determination of the arsenic form are high; and the method is simple to operate, easy to realize and good in reproducibility, has an important practical significance for reasonable evaluation of the food safety, and is suitable for popularization and application in a general laboratory.