Microtube device and using method thereof

Abstract

The invention relates to a microtube device and a using method thereof. The microtube device is characterized by comprising an upper layer of top cap layer and a lower layer of substrate layer, wherein the top cap layer is tightly connected with the surface of the substrate layer; the top cap layer and / or the substrate layer are / is provided with a branch microtube; the branch microtube consists of at least one activity screening tube, a buffer cavity and at least two branch tubes which are symmetrically distributed to the center of the buffer cavity; the buffer cavity is connected with the activity screening tube and the branch tube; meanwhile, the top cap layers corresponding to all the end positions of the branch microtube are provided with a hole respectively to form a sample inlet pool and a sample outlet pool; the sample inlet pools are communicated with the corresponding activity screening tube respectively; and the sample outlet pools are communicated with the corresponding branch tubes respectively. Cells can be cultured in situ in the sample outlet pool and can continuously generate a chemotactic matter by selectively adding cells capable of releasing the chemotactic matter into one or more sample outlet pools, so that uniform and stable chemical gradient fields are generated in the buffer cavity and the branch tubes. The microtube device has the advantages of simple manufacturing steps, low cost, quick and effective screening process, and easy popularization and use in ordinary laboratories.

Description

technical field [0001] The invention relates to a microfluidic chip, in particular to a microchannel device used for cell viability sorting and chemotaxis property detection and its application method. Background technique [0002] Microfluidics (Microfluidics) technology refers to processing a small amount (10 -9 ~10 -18 L) Reaction system of liquid or sample. The application of microfluidic technology in the field of biochemical analysis originated from the research of capillary electrophoresis. Microfluidic technology itself has good characteristics: the ability to manipulate a small amount of samples and reactants, high separation and detection sensitivity, low cost and low energy consumption, fast reaction speed, and high integration. These ensure that experiments can be performed sequentially and efficiently in fewer steps. At present, microfluidic technology has been widely used in multi-level research and analysis from DNA, protein, cell to tissue level. [0003...

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Problems solved by technology

However, the disadvantage of this device is that it is highly dependent on the stability of the fluid, and it is difficult to avoid the influence of the fluid shear force on the sperm during the operation.

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