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Method for germinating ganoderma spores
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A technology of basidiospores and spores, applied in botany equipment and methods, horticulture, applications, etc.
Inactive Publication Date: 2013-04-10
FUJIAN AGRI & FORESTRY UNIV
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[0005] The purpose of the present invention is to provide a method for the germination of the basidiospores of Zizhi, solve the problem that the basidiospores of Zizhi are not easy to germinate, and lay the foundation for the hybrid breeding of Zizhi
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Embodiment 1
[0034] Embodiment 1: A kind of method of Zizhi basidiospore germination
[0035] The cultivar Zizhi S2 of Fujian Zizhi was selected as the parent, activated on the PDA slope, and then transferred to the wood dust bag after the slope was covered. See literature for specific planting methods: Zhong Liyi, Qiu Fuping, Chen Tiqiang. Demonstration experiment of cultivating Zizhi on the sapwood of broad-leaved trees[J]. Edible Fungi, 2005, 1: 26-27.
[0036] 2. Selection of Basidiospore Collection Period
[0037] The release period of Zizhi spores is about 30 days, and the basidiospores used for germination should be selected to be released in the first 10 days. That is to say, when the cap of Zizhi lucidum is differentiated and formed, the selected flower type is complete, the growth is strong, the fungus tube develops normally, the fruiting body grows to 3-5cm, and the basidiospores are collected when the edge of the cap is 0.3-0.6cm into a white growth circle.
[0038] 3. Coll...
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Abstract
The invention relates to a method for germinating ganoderma spores. The method comprises the following steps that ganoderma fruiting bodies having white sides on pileus and just starting to shoot spores are selected for collecting the spores; the spores are collected through a fruiting body movable cap bag; and the spores are applied on a germination culture medium (a prescription is as follows: 200ml of wort, 100ml of ganoderma lucidum extract, 20g of agar, 700ml of water, and sterilization is carried out for 30 minutes at 121DEG C), and a ganoderma mononuclear strain is obtained. The difficult problems that mature ganoderma spores are not easily germinated under laboratory conditions and the crossbreeding of ganoderma is difficult are solved. The method for germinating ganoderma spores is simple and feasible, no special medicine, instruments and equipment need to be used, and the required mononuclear strain for crossbreeding or the genetic analysis of ganoderma can be obtained in an ordinary laboratory.
Description
technical field [0001] The invention relates to a method for germination of basidiospores of Zizhi lucidum. Background technique [0002] Zizhi, scientific name Ganoderma sinense (Zhao, Xu et ) Zhang, belongs to the Phylum Fungi, Basidiomycotina, Phyllomycetes, Phyllomycetes, Ganodermaceae, Ganoderma genus. Zizhi, also known as "Chinese Ganoderma", is mainly distributed in the wild in Guangdong, Guangxi, Fujian and Taiwan. The fruiting body is corky, mostly semicircular to kidney-shaped, a few are nearly round, the length and width of large individuals can reach 20cm, the average individual is 4.7×4cm, and the small individual is 2×1.4cm. The surface is purple-black with a paint-like luster. Bacteria rusty brown. The mouth of the bacteria tube is the same color as the meat of the bacteria, and the mouth of the tube is round, 5 pieces per mm. The stipe is lateral, up to 15cm long, about 2cm in diameter, black and shiny. The top of the basidiospore is umbilical, or sl...
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