Production method for iPS cell-derived genetically diverse T cell colony

Abstract

Provided is a method for producing a genetically diverse regenerated T cell colony via iPS cells, and the regenerated T cell colony. A method for producing a genetically diverse regenerated T cell colony via iPS cells, the method including: (1) a step in which a T cell colony that has tropism toward a target tissue or antigen is concentrated from a sampled genetically diverse T cell colony; (2) astep in which the concentrated T cell colony is reprogrammed into iPS cells and cultured, while maintaining genetic diversity; and (3) a step in which a genetically diverse regenerated T cell colony is produced from the cultured iPS cells.

Description

technical field [0001] The present invention relates to methods for producing genetically diverse regenerative T cell populations and related regenerative T cell populations. Background technique [0002] T cells play a major role in the immune system against foreign pathogens such as bacteria or viruses or abnormal cells such as cancer cells. If the function of T cells decreases for various reasons, it is considered that the subject becomes susceptible to infection, resulting Suffering from cancer etc. For such diseases, if immune cells can be supplemented and regenerated, it will be an extremely effective means for improving the symptoms of the disease, improving the therapeutic effect, and the like. [0003] Complementary therapy using T cells induced from pluripotent stem cells such as iPS cells has been proposed. Using iPS cells as a raw material, by using T cells specific for the target antigen, it is possible to prepare regenerated T cells having the same antigen sp...

AI Technical Summary

Problems solved by technology

On the other hand, in the current T cell supplement therapy, although genetically diverse T cell populations can be used, the expansion of the T cell population in vitro fatigues the T cells, resulting in a decrease in the immune response to antigens.

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