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Sequences of primer for identifying fusarium solani and/or fusarium oxysporum, kit and method thereof

A technology for Fusarium solanacearum and Fusarium oxysporum, which is applied in the field of molecular biology and can solve the problems of identification failure and different quantities.

Active Publication Date: 2015-01-07
NINGBO ACAD OF AGRI SCI
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Problems solved by technology

However, the number of homologous genes of CYP51 gene in different fungi is different, most pathogenic fungi have 1-2 homologous genes of CYP51, only Fusarium oxysporum and Fusarium graminearum have 3 homologous genes of CYP51, so Targeting a CYP51 sequence with multiple homologous genes may result in identification failure

Method used

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  • Sequences of primer for identifying fusarium solani and/or fusarium oxysporum, kit and method thereof
  • Sequences of primer for identifying fusarium solani and/or fusarium oxysporum, kit and method thereof
  • Sequences of primer for identifying fusarium solani and/or fusarium oxysporum, kit and method thereof

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Embodiment Construction

[0026] The present invention is illustrated in detail below through specific embodiments, but the present invention is not limited to the following examples.

[0027] Strain Selection

[0028] 2 Fusarium solani FS-4 and FS-5, 2 F. oxysporum FO-1 and FO-2, F. graminearum, Fusarium half-naked (F. semimitectum), Botrytis cinerea, Didymella bryoniae, Rhizoctonia solani and Sclerotinia sclerotiorum.

[0029] The above-mentioned strains are all preserved in the Vegetable Research Institute of Ningbo Academy of Agricultural Sciences, and can also be obtained from diseased strains such as melons by means of isolation and purification of pathogenic fungi. And the laboratory can generally be separated.

[0030] DNA extraction

[0031] Among the present invention, Fusarium solani, Fusarium oxysporum and other pathogenic fungi all adopt the easy and fast mycelium DNA extraction method, and concrete operation steps are as follows:

[0032] Use a sterilized toothpick to scrape about 100...

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Abstract

Sequences of primers for identifying fusarium solani and / or fusarium oxysporum, a kit and a molecular detection method thereof are disclosed. By verification, the primer sequences, the kit and the method have high specificity. By utilization of the group of the primers and the method, the fusarium solani and the fusarium oxysporum can be identified rapidly and accurately. Establishment of a system has important meaning for rapid early-phase detection of melon root rot and wilt, soil germ distribution and disease control. In addition, establishment of a molecular detection system based in TEF1-alpha as a target gene provides theoretical foundations and identification methods for complex systematic classification and identification of fusarium pathogenic bacteria.

Description

technical field [0001] The invention relates to the field of molecular biology, and develops a primer sequence, a kit and an identification method capable of rapidly identifying Fusarium solani or / and Fusarium oxysporum. Background technique [0002] Fusarium wilt of melons is a worldwide soil-borne vascular disease, which poses a serious threat to the yield and quality of melons. The loss rate of continuous cropping plots reaches more than 30%, and serious plots even fail. Melon wilt is caused by Fusarium oxysporum, which can be divided into seven specialized types according to different hosts on melons. The pathogen can survive on soil and plant residues for a long time, and can occur throughout the growth period of the plant. Once the disease occurs, it is difficult to eradicate, and the application of chemical agents is also difficult to achieve the effect of disease control. With the rapid development of protected sweet melon production, melon root rot has gradually be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11C12R1/77
CPCC12Q1/6895C12Q2600/166
Inventor 严蕾艳王毓洪应泉盛王迎儿宋慧张华峰古斌权
Owner NINGBO ACAD OF AGRI SCI
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